Effects of Accelerated Aging on Soybean Seed Germination Indexes at Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

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Incidence of storage fungi and hydropriming on soybean seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000100005 ◽

2013 ◽

Vol 35 (1) ◽

pp. 35-41 ◽

Cited By ~ 3

Author(s):

Denis Santiago da Costa ◽

Nathalie Bonassa ◽

Ana Dionisia da Luz Coelho Novembre

Keyword(s):

Seed Germination ◽

Moisture Content ◽

Seedling Emergence ◽

Mechanical Damage ◽

Soybean Seed ◽

Accelerated Aging ◽

Soybean Seeds ◽

Storage Fungi ◽

Randomized Experimental Design ◽

Low Incidence

Priming is a technique applicable to seeds of various plant species; however, for soybean seed there is little available information correlating such technique to the storage fungi. The objective of this study was to assess hydropriming on soybeans seeds and correlate this technique to occurrence of such fungi. For this, soon after acquisition the soybean seeds, cv. M-SOY 7908 RR, were characterized by: moisture content, mechanical damage, viability (seed germination and seedling emergence) and seed health. A completely randomized experimental design was used with treatments arranged into a factorial scheme 2 × 2 [2 levels of incidence of storage fungi (low and high) × 2 hydropriming (with and without) ]. After application of treatments, the seeds were analyzed by: moisture content, viability (seed germination and seedling emergence) and vigor (first count and accelerated aging). The hydropriming is beneficial to improve the quality of soybean seeds with low incidence of storage fungi, with increments on speed germination (first count) and seed germination after accelerated aging test. The high incidence of microorganisms can reduce the hydropriming benefits.

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The Use of Electrostatic Field to Improve Soybean Seed Germination in Organic Production

Agronomy ◽

10.3390/agronomy11081473 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1473

Author(s):

Zlatica Mamlic ◽

Ivana Maksimovic ◽

Petar Canak ◽

Goran Mamlic ◽

Vojin Djukic ◽

...

Keyword(s):

Seed Germination ◽

Electrostatic Field ◽

Seed Quality ◽

18Th Century ◽

Soybean Seed ◽

Organic Production ◽

Initial Growth ◽

Physical Treatment ◽

Soybean Seedlings ◽

Dark Green

Soybean production in the system of organic agriculture is not very demanding, and this has been well documented both through experimental results and commercial production. However, one of the biggest problems in organic production is the lack of adequate pre-sowing treatments. Therefore, the aim of this study was to examine the effect of the electrostatic field. This is a physical treatment that was first used for seed treatment in the 18th century but has mostly been neglected since then. Seeds of five soybean genotypes with differently colored seed coats (yellow, green, dark green, brown, and black) were included in this study. The seeds were exposed to different values of direct current (DC) with the following voltages: 0 V (control), 3 V, 6 V, and 9 V, to which the seeds were exposed for 0 min (control), 1 min, and 3 min. After exposing the seeds to the electric field, the physiological properties of seeds and seedlings at the first stage of growth were evaluated. The results show that the effect of the electrostatic field on seed quality depends on the genotype, voltage, and exposure time. The application of DC can be a suitable method for improving seed germination and the initial growth of soybean seedlings. In addition, the results indicate that it is necessary to adjust the DC treatment (voltage and duration of exposure of seeds) to particular genotypes since inadequate treatments may reduce the quality of seeds.

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The expansin EXP1 gene in the elongation zone is induced during soybean embryonic axis germination and differentially expressed in response to ABA and PEG treatments

Seed Science Research ◽

10.1017/s0960258520000379 ◽

2020 ◽

pp. 1-9

Author(s):

Nidia H. Montechiarini ◽

Luciana Delgado ◽

Eligio N. Morandi ◽

Néstor J. Carrillo ◽

Carlos O. Gosparini

Keyword(s):

Cell Wall ◽

Seed Germination ◽

Water Availability ◽

Expression Profiles ◽

Soybean Seed ◽

Embryonic Axis ◽

Growth Potential ◽

Elongation Zone ◽

Degenerate Primers ◽

Embryonic Axes

Abstract During soybean seed germination, the expansive growth potential of the embryonic axes is driven by water uptake while cell wall loosening occurs in cells from the elongation zone (EZ). Expansins are regarded as primary promoters of cell wall remodelling in all plant expansion processes, with the expression profiles of the soybean expansins supporting their cell or tissue specificity. Therefore, we used embryonic axes isolated from whole seed and focused on the EZ to study seed germination. Using a suite of degenerate primers, we amplified an abundantly expressed expansin gene at the EZ during soybean embryonic axis germination, which was identified as EXP1 by in silico analyses. Expression studies showed that EXP1 was induced under germination conditions in distilled water and down-regulated by abscisic acid (ABA), which inhibits soybean germination by physiologically restraining expansion. Moreover, we also identified a time window of ABA responsiveness within the first 6 h of incubation in water, after which ABA lost control of both EXP1 expression and embryonic axis germination, thus confirming the early role of EXP1 in the EZ during this process. By contrast, EXP1 levels in the EZ increased even when germination was impaired by osmotically limiting the water availability required to develop the embryonic axes’ growth potential. We propose that these higher EXP1 levels are involved in the fast germination of soybean embryonic axes as soon as water availability is re-established. Taken together, our results show strong EXP1 expression in the EZ and postulate EXP1 as a target candidate for soybean seed germination control.

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Seed germination of species of the Juan Fernández archipiélago under laboratory conditions

Gayana Botánica ◽

10.4067/s0717-66432007000100007 ◽

2007 ◽

Vol 64 (1) ◽

Cited By ~ 6

Author(s):

Jaime G Cuevas ◽

Javier A Figueroa

Keyword(s):

Seed Germination ◽

Laboratory Conditions

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Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

Journal of Seed Science ◽

10.1590/2317-1545v43249697 ◽

2021 ◽

Vol 43 ◽

Author(s):

Thiago Barbosa Batista ◽

Samara Moreira Perissato ◽

Carlos Henrique Queiroz Rego ◽

Gustavo Roberto Fonseca de Oliveira ◽

Fernando Augusto Henning ◽

...

Keyword(s):

Electrical Conductivity ◽

Linear Regression ◽

Soybean Seed ◽

Accelerated Aging ◽

Storage Period ◽

High Potential ◽

Seed Longevity ◽

Soybean Seeds ◽

Radicle Protrusion ◽

Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

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Research Article Consequences of accelerated aging for DNA integrity and seed germination of cowpea

Genetics and Molecular Research ◽

10.4238/gmr18304 ◽

2019 ◽

Vol 18 (3) ◽

Author(s):

S.E.M. Santos ◽

M.A. Gimenes ◽

J.O.L. de Oliveir Júnior ◽

M .F.Costa ◽

M .F.C.Gomes ◽

...

Keyword(s):

Seed Germination ◽

Accelerated Aging ◽

Dna Integrity ◽

Research Article

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Near-infrared spectroscopy used to predict soybean seed germination and vigour

Seed Science Research ◽

10.1017/s0960258518000119 ◽

2018 ◽

Vol 28 (3) ◽

pp. 245-252 ◽

Cited By ~ 7

Author(s):

Maythem Al-Amery ◽

Robert L. Geneve ◽

Mauricio F. Sanches ◽

Paul R. Armstrong ◽

Elizabeth B. Maghirang ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Seed Germination ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Prediction Models ◽

Soybean Seed ◽

Seed Vigour ◽

Near Infrared Spectra ◽

High Germination ◽

Qualitative Models

AbstractRapid, non-destructive methods for measuring seed germination and vigour are valuable. Standard germination and seed vigour were determined using 81 soybean seed lots. From these data, seed lots were separated into high and low germinating seed lots as well as high, medium and low vigour seed lots. Near-infrared spectra (950–1650 nm) were collected for training and validation samples for each seed category and used to create partial least squares (PLS) prediction models. For both germination and vigour, qualitative models provided better discrimination of high and low performing seed lots compared with quantitative models. The qualitative germination prediction models correctly identified low and high germination seed lots with an accuracy between 85.7 and 89.7%. For seed vigour, qualitative predictions for the 3-category (low, medium and high vigour) models could not adequately separate high and medium vigour seeds. However, the 2-category (low, medium plus high vigour) prediction models could correctly identify low vigour seed lots between 80 and 100% and the medium plus high vigour seed lots between 96.3 and 96.6%. To our knowledge, the current study is the first to provide near-infrared spectroscopy (NIRS)-based predictive models using agronomically meaningful cut-offs for standard germination and vigour on a commercial scale using over 80 seed lots.

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