Natural introgression from cultivated soybean (Glycine max) into wild soybean (Glycine soja) with the implications for origin of populations of semi-wild type and for biosafety of wild species in China

2010 ◽  
Vol 57 (5) ◽  
pp. 747-761 ◽  
Author(s):  
Ke-Jing Wang ◽  
Xiang-Hua Li ◽  
Ji-Jun Zhang ◽  
Hong Chen ◽  
Zhi-Liang Zhang ◽  
...  
Keyword(s):  
Glycine Max ◽  
Wild Species ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Wild Type ◽  
Cultivated Soybean

Conservation of leghemoglobin heterogeneity and structures in cultivated and wild soybean

1985 ◽  
Vol 63 (11) ◽  
pp. 1951-1956 ◽  
Author(s):  
W. H. Fuchsman ◽  
R. G. Palmer
Keyword(s):  
Glycine Max ◽  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Rhizobium Japonicum ◽  
Plant Introductions ◽  
Glycine Max L ◽  
Cultivated Soybean ◽  
Selection Of

The leghemoglobins from a genetically diverse selection of 69 cultivated soybean (Glycine max (L.) Merr.) cultivars and plant introductions and 18 wild soybean (Glycine soja Sieb. & Zucc.) plant introductions all consist of the same set of major leghemoglobins (a, c1, c2, c3), as determined by analytical isoelectric focusing. The conservation of both leghemoglobin heterogeneity and also all four major leghemoglobin structures provides strong circumstantial evidence that leghemoglobin heterogeneity is functional. Glycine max and G. soja produced the same leghemoglobins in the presence of Bradyrhizobium japonicum (Kirchner) Jordan and in the presence of fast-growing Rhizobium japonicum.

scholarly journals Comparison of Phenolic and Flavonoid Compound Profiles and Antioxidant and α-Glucosidase Inhibition Properties of Cultivated Soybean (Glycine max) and Wild Soybean (Glycine soja)

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 813
Author(s):  
Qianru Chen ◽  
Xianxian Wang ◽  
Xiaolong Yuan ◽  
John Shi ◽  
Chengsheng Zhang ◽  
...  
Keyword(s):  
Glycine Max ◽  
Glycine Soja ◽  
Biological Activities ◽  
Wild Soybean ◽  
Flavonoid Compound ◽  
Total Phenolic ◽  
Protective Effects ◽  
Metabolic Pathway Analysis ◽  
Cultivated Soybean ◽  
Phenolic And Flavonoid

Wild soybean (Glycine soja Sieb.et Zucc; WS) has been used as a traditional food in China for many years and contains significantly higher levels of isoflavones than cultivated soybean (Glycine max; CS), but the secondary metabolites, including flavonoids and the phenolic composition differences between them, remain unclear. The results showed that WS possessed significantly higher total phenolic and flavonoid content and exhibited better antioxidant and α-glucosidase inhibition activities as well as excellent protective effects against H2O2-induced oxidative injury in a human endothelial cell line. Through metabolomic analysis, 642 metabolites were identified, and 238 showed differential expression, with 151 upregulated and 87 downregulated. A total of 79 flavonoid compounds were identified, 42 of which were upregulated in WS. 2′-Hydroxygenistein, garbanzol, protocatechuic aldehyde, ligustilide, and resveratrol were the most discriminated compounds in WS. The metabolic pathway analysis of differential metabolites related to the biosynthesis of flavonoids and phenolic acids were the biosynthesis of phenylpropanoids, flavonoids, isoflavonoids, flavones, and flavonols. This study substantially elucidated differences in the content of flavonoids and biological activities between WS and CS, which is useful information for the effective utilization of these two black soybean species in food processing.

scholarly journals GsMAS1 Encoding a MADS-box Transcription Factor Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

2020 ◽  
Vol 21 (6) ◽  
pp. 2004 ◽  
Author(s):  
Xiao Zhang ◽  
Lu Li ◽  
Ce Yang ◽  
Yanbo Cheng ◽  
Zhenzhen Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Constitutive Expression ◽  
Specific Pattern ◽  
Mads Box ◽  
Wild Type ◽  
Aluminum Stress ◽  
Al Stress ◽  
Time Specific

The MADS-box transcription factors (TFs) are essential in regulating plant growth and development, and conferring abiotic and metal stress resistance. This study aims to investigate GsMAS1 function in conferring tolerance to aluminum stress in Arabidopsis. The GsMAS1 from the wild soybean BW69 line encodes a MADS-box transcription factor in Glycine soja by bioinformatics analysis. The putative GsMAS1 protein was localized in the nucleus. The GsMAS1 gene was rich in soybean roots presenting a constitutive expression pattern and induced by aluminum stress with a concentration-time specific pattern. The analysis of phenotypic observation demonstrated that overexpression of GsMAS1 enhanced the tolerance of Arabidopsis plants to aluminum (Al) stress with larger values of relative root length and higher proline accumulation compared to those of wild type at the AlCl3 treatments. The genes and/or pathways regulated by GsMAS1 were further investigated under Al stress by qRT-PCR. The results indicated that six genes resistant to Al stress were upregulated, whereas AtALMT1 and STOP2 were significantly activated by Al stress and GsMAS1 overexpression. After treatment of 50 μM AlCl3, the RNA abundance of AtALMT1 and STOP2 went up to 17-fold and 37-fold than those in wild type, respectively. Whereas the RNA transcripts of AtALMT1 and STOP2 were much higher than those in wild type with over 82% and 67% of relative expression in GsMAS1 transgenic plants, respectively. In short, the results suggest that GsMAS1 may increase resistance to Al toxicity through certain pathways related to Al stress in Arabidopsis.

scholarly journals Genetic Control and Geo-Climate Adaptation of Pod Dehiscence Provide Novel Insights into Soybean Domestication

2019 ◽  
Vol 10 (2) ◽  
pp. 545-554 ◽  
Author(s):  
Jiaoping Zhang ◽  
Asheesh K. Singh
Keyword(s):  
Association Analysis ◽  
Climate Adaptation ◽  
Southern China ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Candidate Gene Association ◽  
Genome Wide ◽  
Cultivated Soybean ◽  
Soybean Domestication ◽  
Pod Dehiscence

Loss of pod dehiscence was a key step in soybean [Glycine max (L.) Merr.] domestication. Genome-wide association analysis for soybean shattering identified loci harboring Pdh1, NST1A and SHAT1-5. Pairwise epistatic interactions were observed, and the dehiscent Pdh1 overcomes resistance conferred by NST1A or SHAT1-5 locus. Further candidate gene association analysis identified a nonsense mutation in NST1A associated with pod dehiscence. Geographic analysis showed that in Northeast China (NEC), indehiscence at both Pdh1 and NST1A were required in cultivated soybean, while indehiscent Pdh1 alone is capable of preventing shattering in Huang-Huai-Hai (HHH) valleys. Indehiscent Pdh1 allele was only identified in wild soybean (Glycine soja L.) accession from HHH valleys suggesting that it may have originated in this region. No specific indehiscence was required in Southern China. Geo-climatic investigation revealed strong correlation between relative humidity and frequency of indehiscent Pdh1 across China. This study demonstrates that epistatic interaction between Pdh1 and NST1A fulfills a pivotal role in determining the level of resistance against pod dehiscence, and humidity shapes the distribution of indehiscent alleles. Our results give further evidence to the hypothesis that HHH valleys was at least one of the origin centers of cultivated soybean.

Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 715-723 ◽  
Author(s):  
P. J. Maughan ◽  
M. A. Saghai Maroof ◽  
G. R. Buss
Keyword(s):  
Genetic Markers ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Allelic Diversity ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Ssr Loci ◽  
Low Levels ◽  
Cultivated Soybean ◽  
Simple Sequence

The objectives of this study were to (i) assess the extent of genetic variation in soybean microsatellites (simple sequence repeats or SSRs), (ii) assay for amplified sequence length polymorphisms (ASLPs), and (iii) evaluate the usefulness of SSRs and ASLPs as genetic markers. Five microsatellites detected a total of 79 variants (alleles) in a sample of 94 accessions of wild (Glycine soja) and cultivated soybean (G. max). F2 segregation analysis of four of the five microsatellites identified these variants (alleles) with four loci located in independent linkage groups. The number of alleles per microsatellite locus ranged from 5 to 21; to our knowledge these are the largest numbers of alleles for single Mendelian loci reported in soybean. Allelic diversity for the SSR loci was greater in wild than in cultivated soybean. Overall, 43 more SSR alleles were detected in wild than in cultivated soybean. These results indicate that SSRs are the marker of choice, especially for species with low levels of variation as detected by other types of markers. Two alleles were detected at each of the three ASLP loci examined. A total of six ASLP alleles were observed in cultivated soybean and five were observed in wild soybean; all alleles detected in wild soybean were present in cultivated soybean. Allelic diversity values for the ASLP loci were near previous estimates for restriction fragment length polymorphisms and therefore ASLPs may be useful as genetic markers in site-directed mapping.Key words: microsatellite, simple sequence repeat, soybean, amplified sequence length polymorphism, genetic mapping.

scholarly journals CRISPR/Cas9-Mediated Targeted Mutagenesis of Wild Soybean (Glycine soja) Hairy Roots Altered the Transcription Profile of the Mutant

2020 ◽  
Vol 12 (9) ◽  
pp. 14
Author(s):  
Fengjuan Niu ◽  
Qiyan Jiang ◽  
Rui Cheng ◽  
Xianjun Sun ◽  
Zheng Hu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Genome Editing ◽  
Hairy Roots ◽  
Glycine Soja ◽  
Differentially Expressed Gene ◽  
Wild Soybean ◽  
Targeted Mutagenesis ◽  
Functional Identification ◽  
Transcription Profiles ◽  
Biallelic Mutations

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated protein 9 (CRISPR/Cas9) system has been regularly applied for genome editing and gene function identification in wild soybean (Glycine max) cultivars. However, till date no studies have demonstrated successful mutagenesis in wild soybean (Glycine soja) which is the ancestor of Glycine max and rich in stress tolerance genes. In the current study, we report the successful creation of mutations in the loci encoding plasma membrane Na+/H+ antiporter (SOS1) and nonselective cation channels (NSCC) in wild soybean hairy roots using the CRISPR/Cas9 system. Two genes, GsSOS1 and GsNSCC, were mutagenized with frequencies of 28.5% and 39.9%, respectively. Biallelic mutations in GsSOS1 were detected in transgenic hairy roots. GsSOS1 mutants exhibited altered Na+/K+ ratios in the roots under both control and salt-treated conditions. However, no significant effects of GsNSCC mutation on Na+/K+ ratios were observed. RNA-Seq analysis revealed that both GsSOS1 and GsNSCC mutation altered the transcription profiles in mutant roots. Many differentially expressed gene sets that are associated with various cellular functions were identified. Our results demonstrated that CRISPR/Cas9 systems as powerful tools for wild soybean genome editing and would significantly advance the gene mining and functional identification in wild soybean.

scholarly journals Evaluation of Glycine max and Glycine soja for Resistance to Calonectria ilicicola

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 887 ◽  
Author(s):  
Chang-Jie Jiang ◽  
Shoji Sugano ◽  
Sunao Ochi ◽  
Akito Kaga ◽  
Masao Ishimoto
Keyword(s):  
Glycine Max ◽  
Disease Severity ◽  
Severity Score ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Fungal Growth ◽  
Disease Severity Score ◽  
Calonectria Ilicicola ◽  
Soybean Cultivars ◽  
The World

Breeding for resistance to soybean red crown rot (Calonectria ilicicola) has long been hampered by the lack of genetic sources of adequate levels of resistance to use as parents. Mini core collections of soybean (Glycine max) originating from Japan (79 accessions), from around the world (80 accessions), and a collection of wild soybeans (Glycine soja) consisting 54 accessions were evaluated for resistance to C. ilicicola (isolate UH2-1). In the first two sets, average disease severity scores of 4.2 ± 0.28 and 4.6 ± 0.31 on a rating scale from zero for no symptom to 5.0 for seedling death were recorded from the set from Japan and the world. No high levels of resistance were observed in these two sets. On the other hand, disease severity score of 3.8 ± 0.35 for the wild soybean accessions was somewhat lower and exhibited higher levels of resistance compared to the soybean cultivars. Three accessions in the wild soybean collection (Gs-7, Gs-9, and Gs-27) had disease severity score ≤2.5 and showed >70% reduction in fungal growth in the roots compared to soybean control cv. “Enrei”. Further analysis using 10 C. ilicicola isolates revealed that accession Gs-9 overall had a wide range of resistance to all isolates tested, with 37% to 93% reduction in fungal growth relative to the cv. Enrei. These highly resistant wild soybean lines may serve as valuable genetic resources for developing C. ilicicola-resistant soybean cultivars.

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