Inhibitory effect of chemical combinations on seed germination and pre-harvest sprouting in hybrid rice

Knowledge remains limited about whether the optimum culture condition of getting the most virulent M.oryzae toxins can be found and applied for resistant seed selection. The inhibition of seed germination and the growth of embryo and radicle in rice treated by the Magnaporthe grisea toxin that got from different culture conditions were studied. The result suggested that the M.oryzae toxin had great inhibitory effect on seed germination and growth of embryo and radicle in rice. The M.oryzae toxin cultivated on 23 °C, pH 8.0 for 20d has strongest inhibition on seed germination of the hybrid rice variety of GangYou725, the inhibition rate approach 70%; Being cultivated at the same pH and 23°C～28°C for approximate 10d, it is easy to get the M.oryzae toxin for high growth inhibition of embryo and radicle. It can reduce the workload and increase accuracy to use M.oryzae toxin to identify blast resistances of rice and select resistant materials.

Download Full-text

Inhibitory effect of eugenol on seed germination and pre-harvest sprouting of hybrid rice (Oryza sativa L.)

Scientific Reports ◽

10.1038/s41598-017-04104-x ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 7

Author(s):

Qijuan Hu ◽

Cheng Lin ◽

Yajing Guan ◽

Mohamed Salah Sheteiwy ◽

Weimin Hu ◽

...

Keyword(s):

Oryza Sativa ◽

Seed Germination ◽

Hybrid Rice ◽

Oryza Sativa L ◽

Inhibitory Effect

Download Full-text

The Effect of High Concentrations of Ethylene on Seed Germination of Douglas Fir (Pseudotsugamenziesii (Mirb.) Franco)

Canadian Journal of Forest Research ◽

10.1139/x75-058 ◽

1975 ◽

Vol 5 (3) ◽

pp. 419-423 ◽

Cited By ~ 4

Author(s):

Carey Borno ◽

Iain E. P. Taylor

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Inhibitory Effect ◽

Germination Percentage ◽

Douglas Fir ◽

Control Treatment ◽

Continuous Exposure ◽

Short Term ◽

Short Term Exposure ◽

High Concentrations

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.

Download Full-text

Allelopathic Potency and an Active Substance from Hairy Vetch

Legume Research - An International Journal ◽

10.18805/lr-548 ◽

2020 ◽

Author(s):

Weihong Sun ◽

Guofeng Yang ◽

Lili Cong ◽

Juan Sun ◽

Lichao Ma

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Root Length ◽

Leaf Extract ◽

Growth Inhibitor ◽

Inhibitory Effect ◽

Allelopathic Effect ◽

Coumaric Acid ◽

Hairy Vetch ◽

Allelopathic Effects

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.

Download Full-text

The counteracting effect of potassium cyanide in sodium azide-inhibited germination of Paulownia tomentosa Steud. seeds

Archives of Biological Sciences ◽

10.2298/abs0501029z ◽

2005 ◽

Vol 57 (1) ◽

pp. 29-34 ◽

Cited By ~ 1

Author(s):

Suzana Zivkovic ◽

Dragoljub Grubisic ◽

Zlatko Giba ◽

Radomir Konjevic

Keyword(s):

Seed Germination ◽

Light Pulse ◽

Sodium Azide ◽

Incubation Medium ◽

Red Light ◽

Potassium Cyanide ◽

Inhibitory Effect ◽

Escape Time ◽

Paulownia Tomentosa ◽

Respiratory Inhibitors

The effect of some respiratory inhibitors on light-induced Paulownia tomentosa Steud. seed germination was studied. Millimolar solution of sodium azide was sufficient to completely prevent germination induced by a 5-min red light pulse. The inhibitory effect of azide was absent if seeds were rinsed before phytochrome activation by light. Sodium azide was effective only if present in the period of Pfr activity. The escape time from azide inhibition compared to the escape from far-red light action, was delayed for about 24 hours. When azide was applied after phytochrome activation, its effect depended on how long it was present in the incubation medium. The removal of azide allowed full restoration of germination by another red light pulse and the far-red escape time did not differ from the escape of untreated, i.e. water-imbibed seeds. Potassium cyanide alone did not produce any effect in light-stimulated germination of these seeds. However, it counteracted the inhibitory effect of azide in light-stimulated germination, if applied simultaneously at a concentration three times higher.

Download Full-text

Physiological changes and sHSPs genes relative transcription in relation to the acquisition of seed germination during maturation of hybrid rice seed

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.7283 ◽

2015 ◽

Vol 96 (5) ◽

pp. 1764-1771 ◽

Cited By ~ 8

Author(s):

Li-Wei Zhu ◽

Dong-Dong Cao ◽

Qi-Juan Hu ◽

Ya-Jing Guan ◽

Wei-Min Hu ◽

...

Keyword(s):

Seed Germination ◽

Hybrid Rice ◽

Rice Seed ◽

Physiological Changes

Download Full-text

Inhibitory effect of rosemary essential oil, loaded in liposomes, on seed germination of Acacia saligna, an invasive species in Mediterranean ecosystems

Botany ◽

10.1139/cjb-2018-0212 ◽

2019 ◽

Vol 97 (5) ◽

pp. 283-291 ◽

Cited By ~ 1

Author(s):

Alfredo Maccioni ◽

Andrea Santo ◽

Danilo Falconieri ◽

Alessandra Piras ◽

Maria Manconi ◽

...

Keyword(s):

Seed Germination ◽

Essential Oil ◽

Invasive Alien Species ◽

Mediterranean Ecosystems ◽

Inhibitory Effect ◽

Acacia Saligna ◽

Southwestern Australia ◽

Rosmarinus Officinalis L ◽

Mediterranean Plant ◽

Germination Requirements

Acacia saligna (Labill.) Wendl. is native of southwestern Australia, but has been planted extensively in many areas of the world, including the Mediterranean region, becoming highly invasive especially in coastal habitats. The aim of this study was to test whether the indigenous Rosmarinus officinalis L. essential oil (EO), loaded in liposomes, can inhibit the seed germination of the invasive alien Acacia saligna. Variability in seed germination requirements and responses among populations were evaluated. Germination tests under light, at constant temperatures and three concentrations of rosemary EO were carried out. Among the examined factors, only the EO amount and temperature had a highly significant effect on seed germination. The lowest EO quantity did not show differences compared with the control, while the highest amount inhibited significantly seed germination of all populations at all the tested temperatures. No seed recovered the ability to germinate after the EO treatment with either of the two washing methods. Our results allowed us to identify the minimum amount of rosemary EO capable of inhibiting the seed germination of the invasive Acacia saligna. These results could be useful for the control of this invasive alien species, thus allowing the conservation of indigenous Mediterranean plant species and habitats.

Download Full-text

CHEMICAL CONSTITUENTS IN WILD OAT (Avenu fatua) HULLS AND THEIR EFFECTS ON SEED GERMINATION

Canadian Journal of Plant Science ◽

10.4141/cjps82-023 ◽

1982 ◽

Vol 62 (1) ◽

pp. 155-161 ◽

Cited By ~ 4

Author(s):

FRANK S. CHEN ◽

JOHN M. MacTAGGART ◽

RICHARD M. ELOFSON

Keyword(s):

Seed Germination ◽

Chemical Constituents ◽

Short Chain Fatty Acids ◽

Inhibitory Effect ◽

Water Soluble ◽

Nonanoic Acid ◽

Lettuce Seed ◽

Wild Oat ◽

Phenolic Inhibitor ◽

Lettuce Seed Germination

Aqueous extracts of dormant wild oat (Avena fatua L.) hulls had a weak inhibitory effect on lettuce seed germination. Both paper and thin layer chromatographic analyses of the ether-soluble acidic fraction showed the presence of phenolics and short-chain fatty acids. No abscisic acid was detected. Vanillin, protochatechualdehyde, p-coumaric acid, ferulic acid and caffeic acid were isolated from hull extracts. Vanillin was shown to be the main water-soluble phenolic inhibitor of lettuce seed germination. At a concentration higher than 6.6 × 10−3M, vanillin was found to be more inhibitory than nonanoic acid to both lettuce and wild oat germination. The concentration of phenolics in the hulls does not account for suppression of wild oat seed germination.

Download Full-text

Inhibitory effect of aqueous extracts of Centaurea solstitialis subsp. schouwii on seed germination and growth of Sulla coronaria

Botany ◽

10.1139/cjb-2019-0108 ◽

2020 ◽

Vol 98 (5) ◽

pp. 273-281

Author(s):

Chadlia Hachani ◽

Mohammed S. Lamhamedi ◽

Mejda Abassi ◽

Zoubeir Béjaoui

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Inhibitory Effect ◽

Centaurea Solstitialis ◽

Aqueous Extracts ◽

Linear Relationships ◽

Allelopathic Effects ◽

Anthropogenic Threats ◽

Sulla Coronaria

Biodiversity has been confronted with anthropogenic threats and several natural threats such as biological invasions. The success of these invasions involves phytotoxic products released by invasive plants that can exhibit allelopathic effects on target species. Thus, aqueous extracts from different parts of the Mediterranean yellow star-thistle [Centaurea solstitialis subsp. schouwii (DC.) Gugler], were tested for their allelopathic effects on seed germination and seedling growth of Sulla coronaria (L.). Bioassays were conducted in vitro to test the effects of the aqueous extracts of shoot, basal and root parts of C. solstitialis subsp. schouwii at two different concentrations (50 g·L−1 and 10 g·L−1). The concentrations of total polyphenols, flavonoids, and tannins of the extracts were also evaluated. Our results showed inhibitory effects on the germination and seedling growth of S. coronaria seedlings, particularly with the extract form the basal part, reaching 84%. This study confirms the linear relationships between the allelopathic effects of C. solstitialis subsp. schouwii and the polyphenol and flavonoid contents. However, further experiments are needed under field conditions to confirm the results obtained under laboratory conditions.

Download Full-text

Stress tolerance responses of two cotton cultivars exposed to ultraviolet-A (366 nm) radiation: photosynthetic performance and chemical constituents

Acta Agronomica Hungarica ◽

10.1556/aagr.52.2004.2.1 ◽

2004 ◽

Vol 52 (2) ◽

pp. 113-124 ◽

Cited By ~ 8

Author(s):

M. K. H. Ebrahim

Keyword(s):

Seed Germination ◽

Stress Tolerance ◽

Chemical Constituents ◽

Photosynthetic Apparatus ◽

Dry Mass ◽

Inhibitory Effect ◽

Photosynthetic Performance ◽

Soluble Proteins ◽

Hill Reaction ◽

Ultraviolet A

The stress tolerance responses of two Egyptian cotton cultivars (Giza 45 and 86) exposed to various doses (40, 80, 160 and 320 min) of artificial ultraviolet-A (366 nm) radiation were investigated. The seed germination of Giza 86 was promoted at 40 min, but substantially inhibited at 80 and 160 min and completely suppressed at 320 min. However, the seed germination of Giza 45 was progressively inhibited by UV-A exposure and ceased at 160 min, so doses of 40 and 80 min were selected for further studies. In contrast to seed germination, the seedling growth of Giza 86 was negatively affected at 40 min. UV-A stress induced a great reduction in the leaf carbohydrates as well as in the viability and dry mass production of the shoots of both cultivars, but the response was comparatively higher in Giza 45. It also decreased the chorophyll (Chl) and carotenoid contents, coupled with an increase in the Chl a/b ratio, diminished the Hill reaction activity, and quenched the Chl a fluorescence both in the presence and absence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea, suggesting an inhibitory effect on the water-splitting system (donor side) as well as on the electron transport from the primary to the secondary acceptors of PSII (acceptor side). These changes reflect a disturbance in the structure, composition and function of the photosynthetic apparatus as well as the sensitivity of PSII to UV-A stress. Nucleic acids (DNA and RNA) were markedly damaged by exposure to UV-A for 80 min, while both cultivars developed adaptive mechanisms for damage moderation. These mechanisms involved increasing the levels of flavonoids, total lipids and total soluble proteins as well as having smaller, thicker leaf blades. Since Giza 86 showed a comparatively higher level of adaptation, it tolerates UV-A stress better than Giza 45. Abbreviations: Car, carotenoids; Chl, chlorophyll; DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DCPIP, 2,6-dichlorophenol indophenol; DNA, deoxyribonucleic acid; d.m., dry mass; f.m., fresh mass; PSII, photosystem II; RNA, ribonucleic acid; TSP, total soluble proteins; UV-AR, ultraviolet-A (366 nm) radiation.

Download Full-text