Research and Application on the Effect of Magnaporthe grisea Toxin on Rice
Knowledge remains limited about whether the optimum culture condition of getting the most virulent M.oryzae toxins can be found and applied for resistant seed selection. The inhibition of seed germination and the growth of embryo and radicle in rice treated by the Magnaporthe grisea toxin that got from different culture conditions were studied. The result suggested that the M.oryzae toxin had great inhibitory effect on seed germination and growth of embryo and radicle in rice. The M.oryzae toxin cultivated on 23 °C, pH 8.0 for 20d has strongest inhibition on seed germination of the hybrid rice variety of GangYou725, the inhibition rate approach 70%; Being cultivated at the same pH and 23°C~28°C for approximate 10d, it is easy to get the M.oryzae toxin for high growth inhibition of embryo and radicle. It can reduce the workload and increase accuracy to use M.oryzae toxin to identify blast resistances of rice and select resistant materials.