Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis

The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has not been completely understood. In this study, the authors performed a time-course transcriptome analysis of the fat body during silkworm metamorphosis using RNA-sequencing. A total of 5217 differentially expressed genes (DEGs) were identified in the fat body at different developmental time points. DEGs involved in lipid synthesis and degradation were highly expressed at the third day of the last larval instar and during the prepupal-pupal transition, respectively. DEGs involved in the ecdysone signaling and bone morphogenetic protein (BMP) signaling pathways that modulate organ development exhibited a high expression level during the fat body remodeling process from prepupa to pupa. Intriguingly, the RNA interference-mediated knockdown of either decapentaplegic (Dpp) or protein 60A (Gbb), two DEGs involved in the BMP signaling pathway, inhibited fat body dissociation but promoted lipid mobilization, suggesting that the BMP signaling pathway not only is required for fat body remodeling, but also moderately inhibits lipid mobilization to ensure an appropriate lipid supply during the pupal-adult transition. In conclusion, the comparative transcriptome analysis provides novel insight into morphologic and metabolic changes in the fat body during silkworm metamorphosis.

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Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA)

PLoS ONE ◽

10.1371/journal.pone.0140609 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0140609 ◽

Cited By ~ 21

Author(s):

Zhengquan Gao ◽

Yan Li ◽

Guanxun Wu ◽

Guoqiang Li ◽

Haifeng Sun ◽

...

Keyword(s):

Salicylic Acid ◽

Jasmonic Acid ◽

Transcriptome Analysis ◽

Haematococcus Pluvialis

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Comparative transcriptome analysis of astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis

10.21203/rs.3.rs-25722/v1 ◽

2020 ◽

Author(s):

Lei Fang ◽

Jingkui Zhang ◽

Zhongnan Fei ◽

Minxi Wan

Keyword(s):

Transcriptome Analysis ◽

Haematococcus Pluvialis ◽

Synthesis Mechanism ◽

Motile Cells ◽

Genes Expression ◽

Malonyl Coa ◽

Astaxanthin Production ◽

Culture Process ◽

Photosynthesis Genes ◽

Accumulation Ability

Abstract Background: Nature astaxanthin is mainly derived from Haematococcus pluvialis. H. pluvialis has four kinds of cell morphology. Based on sequential heterotrophy-dilution-photoinduction (SHDP) technology, photoinduction using non-motile cells as seeds could result in a higher astaxanthin production than that of using brown akinetes as photoinduction seeds. To have a comprehensive understanding of this phenomenon, transcriptome analysis was conducted in this study.Results: Though most of photosynthesis genes expression were down-regulated during the SHDP culture process. Comparing with the group using brown akinetes as photoinduction seeds, the genes expression involved in astaxanthin biosynthesis, lipid biosynthesis and photosynthesis were up-regulated in the non-motile cells group. Especially, chyb gene improving the conversion of β-carotene into astaxanthin was up-regulated by 2.6-fold. The acaca gene enhancing the carboxylation of acetyl-CoA to malonyl-CoA was up-regulated by 1.4-fold.Conclusions: Astaxanthin synthesis mechanism of non-motile cells with higher astaxanthin accumulation ability than brown akinetes was attributed to the up-regulation of astaxanthin metabolism, lipid metabolism and photosynthesis-related genes expression. The results are expected to guide the optimization of astaxanthin production in H. pluvialis by improving lipid content or photosynthesis.

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Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by High Light with Acetate and Fe2+

International Journal of Molecular Sciences ◽

10.3390/ijms19010175 ◽

2018 ◽

Vol 19 (1) ◽

pp. 175 ◽

Cited By ~ 13

Author(s):

◽

...

Keyword(s):

Transcriptome Analysis ◽

Haematococcus Pluvialis ◽

High Light

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Genome-Wide Transcriptome Analysis Reveals Intermittent Fasting-Induced Metabolic Rewiring in the Liver

Dose-Response ◽

10.1177/1559325819876780 ◽

2019 ◽

Vol 17 (3) ◽

pp. 155932581987678 ◽

Cited By ~ 1

Author(s):

Gavin Yong-Quan Ng ◽

Sung-Wook Kang ◽

Joonki Kim ◽

Asfa Alli-Shaik ◽

Sang-Ha Baik ◽

...

Keyword(s):

Transcriptome Analysis ◽

Energy Homeostasis ◽

Molecular Mechanisms ◽

Energy Restriction ◽

Metabolic Changes ◽

Intermittent Fasting ◽

Energy Status ◽

Metabolic Switching ◽

Genome Wide ◽

Transcriptomic Changes

Scope: Intermittent fasting (IF) has been extensively reported to promote improved energy homeostasis and metabolic switching. While IF may be a plausible strategy to ameliorate the epidemiological burden of disease in many societies, our understanding of the underlying molecular mechanisms behind such effects is still lacking. The present study has sought to investigate the relationship between IF and changes in gene expression. We focused on the liver, which is highly sensitive to metabolic changes due to energy status. Mice were randomly assigned to ad libitum feeding or IF for 16 hours per day or for 24 hours on alternate days for 3 months, after which genome-wide transcriptome analysis of the liver was performed using RNA sequencing. Our findings revealed that IF caused robust transcriptomic changes in the liver that led to a complex array of metabolic changes. We also observed that the IF regimen produced distinct profiles of transcriptomic changes, highlighting the significance of temporally different periods of energy restriction. Our results suggest that IF can regulate metabolism via transcriptomic mechanisms and provide insight into how genetic interactions within the liver might lead to the numerous metabolic benefits of IF.

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Accumulation of Astaxanthin Was Improved by the Nonmotile Cells of Haematococcus pluvialis

BioMed Research International ◽

10.1155/2019/8101762 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Feng Li ◽

Minggang Cai ◽

Mingwei Lin ◽

Xianghu Huang ◽

Jun Wang ◽

...

Keyword(s):

Mortality Rate ◽

Cell Cultures ◽

Haematococcus Pluvialis ◽

Morphological Changes ◽

Continuous Light ◽

Motile Cell ◽

Cell Diameter ◽

Batch Mode ◽

Vegetative Cells ◽

Motile Cells

The current commercial production of natural astaxanthin is mainly carried out using Haematococcus pluvialis vegetative cells in the “two-stage” batch mode. The motile vegetative cells are more sensitive to stress than nonmotile vegetative cells, thereby affecting the overall astaxanthin productivity in H. pluvialis cultures. In this study, we compared the differences between motile cells and nonmotile cells in astaxanthin productivity, morphological changes, the mortality rate, and the diameter of the formed cysts. The experimental design was achieved by two different types H. pluvialis cell under continuous light of 80 μmol photons m−2 s−1 for a 9-day induction period. The highest astaxanthin concentration of 48.42 ± 3.13 mg L−1 was obtained in the nonmotile cell cultures with the highest the productivity of 5.04 ± 0.15 mg L−1 day−1, which was significantly higher than that in the motile cell cultures. The microscopic examination of cell morphological showed a large number of photooxidative damaged cells occurring in the motile cell cultures, resulting in higher cell mortality rate (22.2 ± 3.97%) than nonmotile cell cultures (9.6 ± 0.63%). In addition, the analysis results of cell diameter statistics indicated that nonmotile cells were more conducive to the formation of large astaxanthin-rich cysts than motile cells. In conclusion, the works presented here suggest that the accumulation of astaxanthin was significantly improved by nonmotile cells of H. pluvialis, which provided a possibility of optimizing the existing H. pluvialis cultivation strategy for the industrial production.

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