Wastewater treatment using Scenedesmus almeriensis: effect of operational conditions on the composition of the microalgae-bacteria consortia

Author(s):  
Ana Sánchez-Zurano ◽  
Tomás Lafarga ◽  
María del Mar Morales-Amaral ◽  
Cintia Gómez-Serrano ◽  
José María Fernández-Sevilla ◽  
...  
2018 ◽  
Vol 78 (6) ◽  
pp. 1304-1311 ◽  
Author(s):  
I. Mishima ◽  
M. Hama ◽  
Y. Tabata ◽  
J. Nakajima

Abstract Small-scale wastewater treatment plants (SWTPs), called Johkasou, are widely used as decentralized and individual wastewater treatment systems in sparsely populated areas in Japan. Even in SWTPs, nutrients should be removed to control eutrophication. An iron electrolysis method is effective to remove phosphorus chemically in SWTPs. However, it is necessary to determine the precise conditions under which phosphorus can be effectively and stably removed in full scale SWTPs for a long period. Therefore, long-term phosphorus removal from SWTPs was investigated and optimum operational conditions for phosphorus removal by iron electrolysis were analyzed in this study. Efficient phosphorus removal can be achieved for a long time by adjusting the amount of iron against the actual population equivalent. The change of the recirculation ratio had no negative effect on overall phosphorus removal. Phosphorus release to the bulk phase was prevented by the accumulated iron, which was supplied by iron electrolysis, resulting in stable phosphorus removal. The effect of environmental load reduction due to phosphorus removal by iron electrolysis was greater than the cost of power consumption for iron electrolysis.


2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Veronica R. Brand ◽  
Laurel D. Crosby ◽  
Craig S. Criddle

ABSTRACTMultiple clades within a microbial taxon often coexist within natural and engineered environments. Because closely related clades have similar metabolic potential, it is unclear how diversity is sustained and what factors drive niche differentiation. In this study, we retrieved three near-complete Competibacter lineage genomes from activated sludge metagenomes at a full-scale pure oxygen activated sludge wastewater treatment plant. The three genomes represent unique taxa within theCompetibacteraceae. A comparison of the genomes revealed differences in capacity for exopolysaccharide (EPS) biosynthesis, glucose fermentation to lactate, and motility. Using quantitative PCR (qPCR), we monitored these clades over a 2-year period. The clade possessing genes for motility and lacking genes for EPS biosynthesis (CPB_P15) was dominant during periods of suspended solids in the effluent. Further analysis of operational parameters indicate that the dominance of the CPB_P15 clade is associated with low-return activated sludge recycle rates and low wasting rates, conditions that maintain relatively high levels of biomass within the system.IMPORTANCEMembers of the Competibacter lineage are relevant in biotechnology as glycogen-accumulating organisms (GAOs). Here, we document the presence of threeCompetibacteraceaeclades in a full-scale activated sludge wastewater treatment plant and their linkage to specific operational conditions. We find evidence for niche differentiation among the three clades with temporal variability in clade dominance that correlates with operational changes at the treatment plant. Specifically, we observe episodic dominance of a likely motile clade during periods of elevated effluent turbidity, as well as episodic dominance of closely related nonmotile clades that likely enhance floc formation during periods of low effluent turbidity.


2008 ◽  
Vol 591-593 ◽  
pp. 358-361
Author(s):  
J.F. Nunes ◽  
J.R. Lira ◽  
João Jorge Ribeiro Damasceno

The settling vessels are equipment destined to solid-liquid separation; usually have continuous operation, with a circular section, presenting one conic and one cylindrical part. They are very used in chemical industries: wastewater treatment, minerals industries; to concentrate or to remove the solids. The solid particle splitting with small granular becomes difficult through the operation of conventional sedimentation. An expedient very used in the industry is the flocculant substance addition, whose objective is to promote the precipitation of particles, with decantation speed is upper than the single one. The present work aim the study of the burst operational conditions that influence the formation and the stability of these aggregates, the flake, the effect of pH and the concentration of flocculant using kaolin suspension and genfloc, that contains aluminum sulfate, as a flocculant; and the capacity of conventional settling vessel, which area of the transversal remains constant, considering this operational conditions.


Author(s):  
Victor Odhiambo Shikuku ◽  
Wilfrida N. Nyairo

The search for efficient and sustainable wastewater treatment technologies is a subject of continuing research. This is due to the emergence of new classes of water contaminants that are recalcitrant to the conventional wastewater treatment technologies and the stringent allowable limits for contaminant levels set by environmental management authorities. The chapter discusses the developments in synthesis methods and application of polymer-metal oxides as emerging facile materials for wastewater treatment. The varying uses of polymer-metal oxides for different processes in water treatment under varying operational conditions and their performance for different pollutants are critically analyzed. Their strengths and inherent limitations are also highlighted. The chapter demonstrates that polymer-metal oxides are facile low-cost and efficient materials and can be integrated in wastewater and drinking water treatment systems.


2019 ◽  
Vol 80 (12) ◽  
pp. 2352-2361
Author(s):  
L. M. L. Silva ◽  
A. F. Santiago ◽  
G. A. Silva ◽  
A. L. P. Castro ◽  
L. S. Bastos ◽  
...  

Abstract The use of light-emitting diode (LED)-illuminated photobioreactors with microalgae has been extensively studied for wastewater treatment. Most studies have used isolated microalgae species; however, this practice does not match the reality of conditions in wastewater treatment plants. Operational conditions that promote greater growth of algal biomass and that remove pollutants most effectively are disputed in the literature. In this context, LED-illuminated photobioreactors with microalgae were evaluated using multivariate analysis in order to optimize removal of pollutants (nitrogen, phosphorus, and carbonaceous organic matter). Three variables were evaluated: operating time, LED wavelength, and luminous flux intensity. A microalgae consortium was used in the photobioreactor. In addition to the LED-illuminated photobioreactors, control photobioreactors illuminated by sunlight were also operated. Using the results obtained in the optimization, a scaled-up reactor approximately 8.5 times larger in volume was operated to evaluate if the behavior would be maintained. The best operational conditions for the removal of pollutants were observed in LED-illuminated photobioreactors operated under a light intensity of 700 μmol·m−2s−1 for 15 days. Under these conditions, it was possible to remove 89.97% of carbonaceous organic matter, 86.50% of nitrogen, and 30.64% of phosphorus. The scaled-up photobioreactor operated with similar performance.


2002 ◽  
Vol 46 (1-2) ◽  
pp. 551-558 ◽  
Author(s):  
J. van der Waarde ◽  
J. Krooneman ◽  
B. Geurkink ◽  
A. van der Werf ◽  
D. Eikelboom ◽  
...  

Fluorescent In Situ Hybridisation (FISH) was used to monitor the presence of filamentous microorganisms in industrial wastewater treatment plants (WWTPs). Monitoring with a restricted set of FISH probes in WWTPs from potato industry showed growth and decline of Thiothrix populations that could be linked to operational procedures. In a follow up project new FISH probes were developed for filamentous bacteria in industrial WWTPs and 70 WWTPs were analysed for presence of these filaments. Several newly described species of filamentous bacteria appear to be common and dominant in industrial WWTPs. Monitoring of a WWTP from textile industry showed growth and decline of one of these organisms when operational conditions in the plant were varied. The present paper demonstrates that bulking sludge in industrial wastewater treatment plants can effectively be monitored using a combination of standard chemical analyses and the FISH technique.


Author(s):  
Giorgio Mannina ◽  
Alida Cosenza ◽  
Taise Ferreira Rebouças

Abstract This paper presents the sensitivity and uncertainty analysis of a plant-wide mathematical model for wastewater treatment plants (WWTPs). The mathematical model assesses direct and indirect (due to the energy consumption) greenhouse gases (GHG) emissions from a WWTP employing a whole-plant approach. The model includes: i) the kinetic/mass-balance based model regarding nitrogen; ii) two-step nitrification process; iii) N2O formation both during nitrification and denitrification (as dissolved and off-gas concentration). Important model factors have been selected by using the Extended-Fourier Amplitude Sensitivity Testing (FAST) global sensitivity analysis method. A scenario analysis has been performed in order to evaluate the uncertainty related to all selected important model factors (scenario 1), important model factors related to the influent features (scenario 2) and important model factors related to the operational conditions (scenario 3). The main objective of this paper was to analyse the key factors and sources of uncertainty at a plant-wide scale influencing the most relevant model outputs: direct and indirect (DIR,CO2eq and IND,CO2eq, respectively), effluent quality index (EQI), chemical oxygen demand (COD) and total nitrogen (TN) effluent concentration (CODOUT and TNOUT, respectively). Sensitivity analysis shows that model factors related to the influent wastewater and primary effluent COD fractionation exhibit a significant impact on direct, indirect and EQI model factors. Uncertainty analysis reveals that outflow TNOUT has the highest uncertainty in terms of relative uncertainty band for scenario 1 and scenario 2. Therefore, uncertainty of influential model factors and influent fractionation factors has a relevant role on total nitrogen prediction. Results of the uncertainty analysis show that the uncertainty of model prediction decreases after fixing stoichiometric/kinetic model factors.


1997 ◽  
Vol 36 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Tanja Gschlößl ◽  
Ingrid Michel ◽  
Marion Heiter ◽  
Christian Nerger ◽  
Verena Rehbein

In biological wastewater treatment inorganic and organic substances are fixed and metabolized by mixed populations of microorganisms forming either activated sludge flocs or biofilms. Not only the type of wastewater but also the operational conditions promote the development of an adapted biocenosis of microorganisms with specialized enzymatic functions. Understanding the biological properties of the microorganisms, it is possible to assess the prevailing conditions in their natural environment. Regular microscopic and enzymatic investigations of activated sludge and biofilms thus improve the assessment of the stability of the processes and support troubleshooting in wastewater treatment plants. While the role of bacteria is often discussed, the importance of ciliated protozoes and metazoes for the maintenance of the stability of biofilm systems is rarely mentioned. In this paper we intend to show some new results of direct microscopic observations in different sorts of biofilm systems focussing upon ciliated protozoes and metazoes. Practical results will demonstrate the relation between enzymatic analysis, microscopic investigations and performance of biofilm systems.


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