scholarly journals Macrophage-like THP-1 cells show effective uptake of silica nanoparticles carrying inactivated diphtheria toxoid for vaccination

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Xinyue Huang ◽  
Danielle Paixão Cavalcante ◽  
Helen E Townley
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Porous Silica ◽  
Diphtheria Toxoid ◽  
Similar Distribution ◽  
Antigen Delivery ◽  
Before And After ◽  
Post Synthesis

AbstractNanoparticles may be used in vaccinology as an antigen delivery and/or an immunostimulant to enhance immunity. Porous silica has been identified as an effective adjuvant for more than a decade, and we have therefore investigated the take up rate by an immortalized macrophage-like cell line of a number of mesoporous silica nanoparticles (MSNPs) with differing diameter and pore size. The MSNPs were synthesized using a sol-gel reaction and post-synthesis removal of the template. The MSNPs showed a clear distribution in take up rate peaking at 217 nm, whereas a comparison with solid spherical nanoparticles showed a similar distribution peaking at 377 nm. The MSNPs were investigated before and after loading with antigen. Diphtheria toxoid was used as a proof-of-concept antigen and showed a peak macrophage internalization of 53.42% for loaded LP3 particles which had a diameter of 217.75 ± 5.44 nm and large 16.5 nm pores. Optimal MSNP sizes appeared to be in the 200–400 nm range, and larger pores showed better antigen loading. The mesoporous silica particles were shown to be generally biocompatible, and cell viability was not altered by the loading of particles with or without antigen.

scholarly journals Evaluating biological activity of folic acid-modified and 10-hydroxycamptothecin-loaded mesoporous silica nanoparticles

2021 ◽  
Author(s):  
Mei-Xia Zhao ◽  
Di-Feng Chen ◽  
Xue-Jie Zhao ◽  
Lin-Song Li ◽  
Yong-Fang Liu
Keyword(s):  
Folic Acid ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Folate Receptor ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Carriers ◽  
Transmission Electron ◽  
Before And After ◽  
Drug Efficiency

Targeted nanocarrier can selectively deliver anti-tumor drugs to cancer sites improving drug efficiency. Accordingly, a targeted nanocarrier (MSN-FA) was synthesized based on folic acid (FA) modified mesoporous silica nanoparticles (MSNs). These loaded with 10-hydroxycamptothecin (HCPT) to obtain the nano-drug MSN-FA@HCPT. These nanocarriers were characterized by transmission electron microscopy (TEM), zeta potential, ultraviolet-visible spectroscopy (UV-Vis), fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). Notably, the nanocarriers were nearly spherical before and after loading HCPT and exhibited good dispersibility. Also, folate receptor (FR) over-expressing HeLa cells and FR deficient HepG2 cells were used to evaluate in vitro cellular uptake and cytotoxicity of MSN-FA@HCPT and MSN@HCPT. Interestingly, FA-modified nanocarriers enhanced the cytotoxicity of HCPT by improving drug targeting to tumor cells. Also, apoptotic and mitochondrial membrane potential (MMP) reducing effects of MSN-FA@HCPT were more prominent than the MSNs without FA modification. MSN-FA@HCPT can be excellent drug carriers with profound biomedical applications.

scholarly journals Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 332 ◽  
Author(s):  
Saher Rahmani ◽  
Jelena Budimir ◽  
Mylene Sejalon ◽  
Morgane Daurat ◽  
Dina Aggad ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Cathepsin D ◽  
Breast Cancer Cells ◽  
Potent Inhibitor ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Pepstatin A

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.

A new method for loading mesoporous silica nanoparticles with drugs: Sol–gel synthesis using drug micelles as a template

2016 ◽  
Vol 78 (5) ◽  
pp. 586-595 ◽  
Author(s):  
O. V. Dement’eva ◽  
I. N. Senchikhin ◽  
M. E. Kartseva ◽  
V. A. Ogarev ◽  
A. V. Zaitseva ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
New Method ◽  
Sol Gel Synthesis

scholarly journals The Engineering of Porous Silica and Hollow Silica Nanoparticles to Enhance Drug-loading Capacity

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 805 ◽  
Author(s):  
Ngoc-Tram Nguyen-Thi ◽  
Linh Phuong Pham Tran ◽  
Ngoc Thuy Trang Le ◽  
Minh-Tri Cao ◽  
The-Nam Tran ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Loading ◽  
Sol Gel ◽  
Porous Silica ◽  
High Capacity ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Hollow Mesoporous Silica ◽  
Hollow Silica Nanoparticles

As a promising candidate for expanding the capacity of drug loading in silica nanoplatforms, hollow mesoporous silica nanoparticles (HMSNs) are gaining increasing attention. In this study, porous nanosilica (PNS) and HMSNs were prepared by the sol-gel method and template assisted method, then further used for Rhodamine (RhB) loading. To characterize the as-synthesized nanocarriers, a number of techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), nitrogen absorption-desorption isotherms, dynamic light scattering (DLS), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR) were employed. The size of HMSN nanoparticles in aqueous solution averaged 134.0 ± 0.3 nm, which could be adjusted by minor changes during synthesis, whereas that of PNS nanoparticles was 63.4 ± 0.6 nm. In addition, the encapsulation of RhB into HMSN nanoparticles to form RhB-loaded nanocarriers (RhB/HMSN) was successful, achieving high loading efficiency (51.67% ± 0.11%). This was significantly higher than that of RhB-loaded PNS (RhB/PNS) (12.24% ± 0.24%). Similarly, RhB/HMSN also possessed a higher RhB loading content (10.44% ± 0.02%) compared to RhB/PNS (2.90% ± 0.05%). From those results, it is suggested that prepared HMSN nanocarriers may act as high-capacity carriers in drug delivery applications.

Fabrication of mesoporous silica nanoparticles by sol gel method followed various hydrothermal temperature

2018 ◽  
Author(s):  
Hariyati Purwaningsih ◽  
Vania Mitha Pratiwi ◽  
Siti Annisa Bani Purwana ◽  
Haniffudin Nurdiansyah ◽  
Yenny Rahmawati ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Hydrothermal Temperature ◽  
Gel Method ◽  
Sol Gel Method

Enzymatic activity of Fe-grafted mesoporous silica nanoparticles: an insight into H2O2and glucose detection

2018 ◽  
Vol 42 (19) ◽  
pp. 16060-16068 ◽  
Author(s):  
M. Aghayan ◽  
A. Mahmoudi ◽  
M. Reza Sazegar ◽  
N. Ghavidel Hajiagha ◽  
K. Nazari
Keyword(s):  
Mesoporous Silica ◽  
Enzymatic Activity ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Glucose Detection ◽  
Post Synthesis ◽  
Insight Into

Iron-grafted MSNs were synthesized by post-synthesis and applied as a biosensor for detection of glucose and H2O2.

Preparation of Mesoporous Silica Nanoparticles Modified by Urushiol and Their Adsorption on Malachite Green

2021 ◽  
Vol 21 (9) ◽  
pp. 4792-4796
Author(s):  
Xue-Qing Xiao ◽  
Zhi-Yu Chen ◽  
Jia-Wen Chen ◽  
Qin-Hui Chen
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Malachite Green ◽  
Negative Impact ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Dye Adsorption ◽  
Chemical Properties ◽  
Adsorption Rate ◽  
Adsorption Method

The presence of malachite green dye in wastewater has a great negative impact on the environment. At present, industrial wastewater is treated using adsorption, electrolysis and membrane separation, among which the adsorption method is the most widely used wastewater treatment. In this study mesoporous silica nanoparticles (MSNs) were prepared using the sol-gel method and modified with the natural polymer urushiol (U) to obtain MSN@Us, which have a core-shell structure. This is the first use of urushiol in dye adsorption. The structures and chemical properties of the MSNs and MSN@Us were characterized. The adsorption of malachite green by the MSNs and MSN@Us showed that the adsorption rate of MSN@Us was higher than that of MSNs, with an adsorption rate greater than 90%. This study provides a new research direction for the use of urushiol in the treatment of contaminated wastewater.

Recent Advances in Mesoporous Silica Nanoparticles for Targeted Drug Delivery applications

2021 ◽  
Vol 18 ◽  
Author(s):  
Ahmed Abu-Dief ◽  
Mosa Alsehli ◽  
Abdullah Al-Enizi ◽  
Ayman Nafady
Keyword(s):  
Drug Delivery ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
High Surface ◽  
Functional Materials ◽  
Chemotherapeutic Agents ◽  
Antigen Delivery ◽  
Biological Compatibility ◽  
Wide Range

: Nanotechnology provides the means to design and fabricate delivery vehicles capable of overcoming physiologically imposed obstacles and undesirable side effects of systemic drug delivery. This protocol allows maximal targeting effectiveness and therefore enhances therapeutic efficiency. In recent years, mesoporous silica nanoparticles (MSNPs) have sparked interest in the nanomedicine research community, particularly for their promising applications in cancer treatment. The intrinsic physio-chemical stability, facile functionalization, high surface area, low toxicity, and great loading capacity for a wide range of chemotherapeutic agents make MSNPs very appealing candidates for controllable drug delivery systems. Importantly, the peculiar nanostructures of MSNPs enabled them to serve as an effective drug, gene, protein, and antigen delivery vehicle for a variety of therapeutic regimens. For these reasons, in this review article, we underscore the recent progress in the design and synthesis of MSNPs and the parameters influencing their characteristic features and activities. In addition, the process of absorption, dissemination, and secretion by injection or oral management of MSNPs are also discussed, as they are key directions for the potential utilization of MSNPs. Factors influencing the in vivo fate of MSNPs will also be highlighted, with the main focus on particle size, morphology, porosity, surface functionality, and oxidation. Given that combining other functional materials with MSNPs may increase their biological compatibility, monitor drug discharge, or improve absorption by tumor cells coated MSNPs; these aspects are also covered and discussed herein.

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