In vitro morphogenesis from cotyledon and epicotyl explants and flow cytometry distinction between landraces of Bambara groundnut [Vigna subterranea (L.) Verdc], an under-utilised grain legume

2007 ◽  
Vol 88 (1) ◽  
pp. 61-75 ◽  
Author(s):  
M. Koné ◽  
E. M. Patat-Ochatt ◽  
C. Conreux ◽  
R. S. Sangwan ◽  
S. J. Ochatt
Keyword(s):  
Flow Cytometry ◽  
Bambara Groundnut ◽  
Grain Legume ◽  
Vigna Subterranea ◽  
In Vitro Morphogenesis

scholarly journals In VitroSeeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea(L.) Verdc. (Fabaceae))

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mongomaké Koné ◽  
Tchoa Koné ◽  
Nakpalo Silué ◽  
André Brahima Soumahoro ◽  
Tanoh Hilaire Kouakou
Keyword(s):  
Basal Medium ◽  
Medium Composition ◽  
Bambara Groundnut ◽  
Sub Saharan Africa ◽  
High Rate ◽  
Grain Legume ◽  
Vigna Subterranea ◽  
Regeneration System ◽  
Growth Performances

Bambara groundnut (Vigna subterranea(L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficientin vitroregeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three types of seeds were inoculated on different basal media devoid of growth regulators. Various strengths of the medium of choice and the type and concentration of carbon source were also investigated. Responses to germination varied with the type of seed. Embryonic axis (EA) followed by seeds without coat (SWtC) germinated rapidly and expressed a high rate of germination. The growth performances of plantlets varied with the basal medium composition and the seeds type. The optimal growth performances of plants were displayed on half strength MS basal medium with SWtC and EA as source of seeds. Addition of 3% sucrose in the culture medium was more suitable for a maximum growth of plantlets derived from EA.

scholarly journals Effect of Soaking and Boiling on Anti-nutritional Factors, Oligosaccharide Contents and Protein Digestibility of Newly Developed Bambara Groundnut Cultivars

2017 ◽  
Vol 5 (9) ◽  
pp. 1006 ◽  
Author(s):  
Olaposi Adeleke ◽  
Oladipupo Qudus Adiamo ◽  
Olumide Samson Fawale ◽  
Gbeminiyi Olamiti
Keyword(s):  
Protein Digestibility ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Nutritional Factors ◽  
Nutritive Quality ◽  
Vitro Protein

Newly developed Bambara groundnut (Vigna subterranea L.) seeds (Accessions No: TVSU 5 – Bambara Groundnut White (BGW) and TVSU 146 – Bambara Groundnut Brown (BGB)) were collected from International Institute of Tropical Agriculture (IITA), Nigeria, planted and harvested. The effects of processing methods (soaking and boiling) on anti-nutritional factors and oligosaccharides content and protein digestibility of BGW and BGB compared with Bambara groundnut commercial (BGC) seeds were investigated. Soaking and boiling significantly reduced the anti-nutritional factors of the samples and the effect increased as processing time was elongated. Sample BGC had lower anti-nutritional factors than BGW and BGB after soaking for 48 h. Tannin contents of the samples were reduced drastically by 99 % throughout the soaking periods. Greatest loss in raffinose level was observed in BGB (59%) and BGW (50%) after boiling for 60 min compared with BGC (43%). The loss in stachyose content of the samples varies with processing and BGC (59%) had greatest loss after boiling for 60 min while soaking for 48 h reduced that of BGB and BGW by 57 and 35%, respectively. Boiling for 60 min increased the in vitro protein digestibility of BGB (89.34 %) compared with BGW (87.48%) and BGC (82.89%). Overall, the results demonstrated that soaking and boiling of newly developed Bambara groundnut seeds could improve the nutritive quality of the seeds.

scholarly journals Bambara Groundnut (Vigna subterranea [L.] Verdc.) Production, Utilisation and Genetic Improvement in Sub-Saharan Africa

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1345
Author(s):  
Nomathemba Gloria Majola ◽  
Abe Shegro Gerrano ◽  
Hussein Shimelis
Keyword(s):  
Nutritional Quality ◽  
Genetic Improvement ◽  
Market Access ◽  
Crop Management ◽  
Bambara Groundnut ◽  
Sub Saharan Africa ◽  
High Yield ◽  
Grain Legume ◽  
Vigna Subterranea ◽  
Sub Saharan

Bambara groundnut (Vigna subterranea [L.] Verdc.) is a nutritionally rich grain legume crop indigenous to Africa. It is tolerant to drought stress and has become adapted to grow under low input and marginal agricultural production systems in Africa and Asia. Bambara groundnut is an orphan crop, and represents a neglected and under researched plant genetic resource. Modern crop management, production technologies, and value chains are yet to be developed in Africa to achieve the potential economic gains from Bambara groundnut production and marketing. In sub-Saharan Africa (SSA) the production and productivity of Bambara groundnut is low and stagnant because of diverse abiotic and biotic stresses and socio-economic constraints. Improved crop management and post handling technologies, modern varieties with high yield and nutritional quality, value addition, and market access are among the key considerations in current and future Bambara groundnut research and development programs. This paper presents progress on Bambara groundnut production, utilization, and genetic improvement in SSA. It presents the key production constraints, genetic resources and analysis, breeding methods and genetic gains on yield, and nutritional quality and outlook. The information presented will guide the sustainable production and effective breeding of the crop in order to pursue food and nutrition security, and improve livelihoods through Bambara groundnut enterprises.

INFLUENCE OF SOWING DATE AND ENVIRONMENTAL FACTORS ON THE DEVELOPMENT AND YIELD OF BAMBARA GROUNDNUT (VIGNA SUBTERRANEA) LANDRACES IN A SUB-TROPICAL REGION

2008 ◽  
Vol 44 (2) ◽  
pp. 167-183 ◽  
Author(s):  
A. SESAY ◽  
C. N. MAGAGULA ◽  
A. B. MANSUETUS
Keyword(s):  
Environmental Factors ◽  
Rural Areas ◽  
Field Experiments ◽  
Sowing Date ◽  
Bambara Groundnut ◽  
Effect Of Temperature ◽  
Grain Legume ◽  
Tropical Region ◽  
Vigna Subterranea ◽  
Legume Crop

SUMMARYBambara groundnut (Vigna subterranea) is a grain legume crop, which is increasingly popular as food in rural areas across the African continent. However, reliable information pertaining to management of the crop is limited. Field experiments were conducted in Swaziland in the 1998/99 and 1999/2000 cropping seasons to determine the influence of sowing date and environmental factors on the growth, development and yield of bambara groundnut. In the 1998/99 season, seeds of one local landrace were sown on six dates between mid-September 1998 and mid-February 1999. In 1999/2000, seeds of two local landraces were sown on six dates between mid-October 1999 and late January 2000. The highest pod yield and total dry matter production were achieved in November sowings, with maximum pod yields of 1.3 and 0.64 t ha−1 for the 1998/99 and 1999/2000 seasons, respectively. Earlier sowing and successive delays in sowing from November caused substantial yield declines of as much as 72–75%. Sowing date influenced yields of bambara groundnut through the effect of temperature and daylength on plant development. For the landraces used in the study, while the rate of progress from sowing to flowering was influenced by temperature, the rate of progress from flowering to podding was influenced largely by daylength. The practical implications of the results for increased bambara groundnut production are discussed.

In vitro shoot tip multiplication of bambara groundnut [Vigna subterranea (L.) Verdc.]

2016 ◽  
Vol 127 (3) ◽  
pp. 603-611 ◽  
Author(s):  
Nakpalo Silué ◽  
Tchoa Koné ◽  
André Brahima Soumahoro ◽  
Mongomaké Koné
Keyword(s):  
Bambara Groundnut ◽  
Shoot Tip ◽  
Vigna Subterranea ◽  
Shoot Tip Multiplication

scholarly journals Phytochemical composition and antioxidant properties of methanolic extracts of whole and dehulled Bambara groundnut (Vigna subterranea) seeds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bukola C. Adedayo ◽  
Tonna A. Anyasi ◽  
Malcolm J. C. Taylor ◽  
Fanie Rautenbauch ◽  
Marilize Le Roes-Hill ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Seed Extract ◽  
Bambara Groundnut ◽  
Anthocyanin Content ◽  
Vigna Subterranea ◽  
Antioxidant Power ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

AbstractThe distribution of phytochemicals and their contribution to antioxidant potentials in whole and dehulled Bambara groundnut (BGN) seeds was evaluated. Whole BGN seeds were sorted using the testa and hilium colour and further grouped into whole and dehulled BGN seeds. Extractions of both whole and dehulled BGN seeds was done using methanol and the extracts assayed for total phenolics (TPC), flavanol, flavonol, anthocyanin content, oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP). Methanolic extract of whole BGN seed exhibited higher flavanol and flavonol content as well as significantly higher in-vitro antioxidant activities than dehulled BGN seeds. The TPC of whole BGN seed extract ranged from 3.6 to 11.0 GAE/g, while that of dehulled BGN ranged from 2.7 to 3.2 GAE/g. Identification of phenolics in whole and dehulled BGN seed extract using UPLC-qTOF-MS, revealed the presence of monoterpenoids (iridoids), phenolic acids, flavonoids and lignans. Bivariate correlations showed anthocyanin demonstrated weak positive correlation between flavanol, flavonol and ORAC for whole BGN seed extract; and negative correlation between flavanol, TPC, FRAP and ORAC for dehulled BGN. Aside the effect of dehulling, whole BGN seeds exhibited the presence of phytochemicals with beneficial properties for food and industrial application.

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

1999 ◽  
Vol 19 (03) ◽  
pp. 134-138
Author(s):  
Gitta Kühnel ◽  
A. C. Matzdorff
Keyword(s):  
Flow Cytometry ◽  
Platelet Count ◽  
Blood Sample ◽  
Platelet Activation ◽  
Whole Blood ◽  
Receptor Occupancy ◽  
Aggregate Formation ◽  
Inhibitor Activity ◽  
Receptor Inhibitor

SummaryWe studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Proteins ◽  
Electrophoretic Analysis ◽  
Protein Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Phylogenetic Relatedness ◽  
Molecular Weights ◽  
National Centre ◽  
Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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