Effect of heavy-metal-resistant bacteria on enhanced metal uptake and translocation of the Cu-tolerant plant, Elsholtzia splendens

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized.

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Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Güllük Bay, Turkey : Quantifying the resistance of identified bacteria species with potential for environmental remediation applications

Johnson Matthey Technology Review ◽

10.1595/205651320x15953337767424 ◽

2020 ◽

Vol 64 (4) ◽

pp. 507-525 ◽

Cited By ~ 1

Author(s):

Gülşen Altuğ ◽

Mine Çardak ◽

Pelin Saliha Çiftçi Türetken ◽

Samet Kalkan ◽

Sevan Gürün

Keyword(s):

Heavy Metal ◽

Aegean Sea ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Diffusion Method ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Heavy Metal Resistant ◽

Seawater Samples

Heavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK® 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.

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Cd immobilization and reduced tissue Cd accumulation of rice (Oryza sativa wuyun-23) in the presence of heavy metal-resistant bacteria

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2016.12.024 ◽

2017 ◽

Vol 138 ◽

pp. 56-63 ◽

Cited By ~ 45

Author(s):

Ya Li ◽

Hai-Dong Pang ◽

Lin-Yan He ◽

Qi Wang ◽

Xia-Fang Sheng

Keyword(s):

Heavy Metal ◽

Oryza Sativa ◽

Resistant Bacteria ◽

Cd Accumulation ◽

Heavy Metal Resistant

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Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil

Plant and Soil ◽

10.1007/s11104-004-0517-8 ◽

2005 ◽

Vol 269 (1-2) ◽

pp. 225-232 ◽

Cited By ~ 79

Author(s):

Fayuan Wang ◽

Xiangui Lin ◽

Rui Yin

Keyword(s):

Heavy Metal ◽

Contaminated Soil ◽

Metal Uptake ◽

Arbuscular Mycorrhizas ◽

Heavy Metal Uptake ◽

Elsholtzia Splendens

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Heavy metal (As, Cd, Cr, Hg, Pb) analysis and identification of heavy metal resistant bacteria in sediments from Manado Bay

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/926/1/012096 ◽

2021 ◽

Vol 926 (1) ◽

pp. 012096

Author(s):

B J Kepel ◽

W Bodhi ◽

Fatimawali ◽

T E Tallei

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Average Concentration ◽

Resistant Bacteria ◽

Icp Oes ◽

Sediment Samples ◽

Heavy Metal Concentrations ◽

The World ◽

Heavy Metal Resistant ◽

16Srrna Gene

Abstract Environmental pollution from heavy metals is becoming a growing concern due to the adverse effects it is causing throughout the world. This study aims to analyze heavy metal concentrations and identify heavy metal resistant bacteria in the bay of Manado. Sediment samples were collected from five bays in Manado. The concentrations of heavy metals As, Cd, Cr, Hg and Pb were analyzed using ICP-OES, and Hg using CV-AFS. Bacteria from the sediment were grown in nutrient broth media containing heavy metals As, Cd, Cr, Hg and Pb respectively. Microbiology and 16SrRNA gene analysis were used to identify the bacteria that grown on media containing varying concentrations of heavy metals. The results showed that the sediments from the five bays in Manado contained heavy metals with an average concentration of As <1mg/kg, Cd 1.8mg/kg), Cr 6.2mg/kg, Hg <0.07mg/kg). and Pb 11.2mg/kg. The results of microbiological and molecular analysis showed that 5 species of heavy metal resistant bacteria were Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus arlettae, Acinobacter sp., and Brevibacterium sp. The five bacteria found to be resistant to heavy metals can be used to detoxify As, Cd, Cr, Hg, and Pb.

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Characterization of Binding Sites and Optimization of Cell Free Bacteria Condition for Metal Bio-Sorbents

10.20944/preprints202103.0618.v1 ◽

2021 ◽

Author(s):

Dr. Mostafa G. Fadl ◽

Zenat Kamel Mohamed

Keyword(s):

Heavy Metal ◽

Metal Ions ◽

Binding Sites ◽

Metal Uptake ◽

High Efficiency ◽

Bacterial Biomass ◽

Resistant Bacteria ◽

Bacterial Cells ◽

Isolation And Identification ◽

Charge Interaction

Bacteria a Microscopic organisms are the most inexhaustible and flexible of microorganisms and constitute a huge division of the whole living earthly biomass, certain microorganisms were found to amass metallic components at a high limit Was Known as Bacterial Bio-sorption Due to their little size, capacity to become under controlled conditions, and their Accommodation to an extensive variety of ecological situations; Potent metal bio-sorbents among microorganisms, at low pH esteems, cell divider ligands are protonated and contend essentially with metals for official. With expanding pH, more ligands, such as amino and carboxyl groups, could be exposed, leading to attraction between these negative charges and the metals, and consequently increment bio-sorption onto the cell surface. Starting with Isolation and identification of heavy metal-resistant bacteria from rock Ore. Studying Factors Affecting Uranium Bio-sorption, Optimization of bacterial growth conditions and optimum for metal uptake by free and immobilized bacterial cells and Desorption ratio of uranium ions adsorbed by Coli. /alginate, All this evidence suggest that functions groups Represented in our study are responsible for metal uptake in our bacterial biomass beside change in peaks position which assigned for it's groups confirm bio-sorption of metal ions from waste due to ions charge interaction comparing with immobilized we found increase in no of binding sites indicate that immobilized bacterial have high efficiency for metal up take which also change in peaks position which assigned for its groups confirm bio-sorption of metal ions from waste due to ions charge interaction, Where the high bio-sorption yield obtained by bacteria, the Uranium & heavy metal bioremediation process expects microorganisms to be joined to a strong surface.

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