Microbiological Assessment of Chronic Otitis Media: Aerobic Culture Isolates and Their Antimicrobial Susceptibility Patterns

Author(s):  
Mehtab Alam ◽  
Asfia Sultan ◽  
Kamlesh Chandra
1996 ◽  
Vol 38 (5) ◽  
pp. 329-336 ◽  
Author(s):  
Cibele Barreto Mano de Carvalho ◽  
José Luciano Bezerra Moreira ◽  
Maria Candida S. Ferreira

Epidemiological aspects and the antimicrobial susceptibility profile of the Bacteroides fragilis group isolated from clinical and human intestinal specimens were examined in this study. B. fragilis group strains were isolated from 46 (37%) of 124 clinical specimens and the source of the samples was: Blood culture (3), intraabdominal infection (27), brain abscess (2), soft tissue infection (17), respiratory sinus (3), pleural aspirate (9), breast abscess (3), surgical infected wound (22), pelvic inflammatory disease (22), chronic otitis media (9) and miscellaneous (7). Intraabdominal and soft tissue infections were responsible for more than half of the clinical isolates. Susceptibility to penicillin, cefoxitin, tetracycline, metronidazole, chloramphenicol and clindamycin was examined. All isolates were susceptible to metronidazole and chloramphenicol. For clindamycin and cefoxitin the resistance rates observed were 21.7% and 10.9% respectively. Susceptibility profiles varied among the different species tested. A total of 37 species of B. fragilis group isolated from intestinal microbiota of individuals who had no antimicrobial therapy for at least 1 month before the sampling was also examined. All strains were also susceptible to chloramphenicol and motronidazole and the resistance rates to clindamycin and cefoxitin were 19.4% and 5.4% respectively. A few institutions, in Brazil, have monitored the antimicrobial susceptibility of B. fragilis group strains isolated from anaerobic infections. The resistance rates to cefoxitin and clindamycin and the variation in susceptibility patterns among the species isolated in this study emphasize the need for monitoring of susceptibility patterns of B. fragilis group organisms isolated, especially at our University Hospitals.


2019 ◽  
Vol 185 (19) ◽  
pp. 598-598 ◽  
Author(s):  
Lorenzo Pisello ◽  
Elisa Rampacci ◽  
Valentina Stefanetti ◽  
Francesca Beccati ◽  
Doreene Rose Hyatt ◽  
...  

This study aimed to describe bacteria isolated from the reproductive tract of mares and to identify changes in antimicrobial susceptibility patterns to those antibiotics commonly used for the treatment of equine endometritis. A total of 4122 equine uterine swabs were collected from mares suffering from reproductive tract disorders in the period 2010–2017. Aerobic culture and antimicrobial susceptibility testing using agar disc diffusion were performed on each sample. Aerobic bacteria were isolated from 3171 of 4122 (76.9 per cent) samples. The most frequently isolated microorganisms were Escherichia coli (885/3171, 27.9 per cent) and Streptococcus equi subspecies zooepidemicus (791/3171, 24.9 per cent), confirming previous findings from the literature. Antimicrobial susceptibility patterns of E coli, S equi subspecies zooepidemicus and Klebsiella pneumoniae changed over time. A statistically significant decrease in antimicrobial efficacy of cefquinome against E coli was observed over the years, as well as of ampicillin, cefquinome and penicillin against S equi subspecies zooepidemicus. The high frequency of resistant bacteria isolated in the present work proceeds in the same way as indicated by surveillance data on the huge antibiotic use in Italy. As a result, testing and monitoring programmes of antimicrobial efficacy are crucial to consciously using antibiotics and preserving their effectiveness both for veterinary and human medicine.


Author(s):  
Qasem Buhaibeh ◽  
Tawfik Dhaiban ◽  
Eiman Alyafei ◽  
Amjad Salman Al-Jundi Abdulsalam

Background: Otitis Media (OM) common diseases affecting children below 5 years of age with a harmful effect on their hearing and health status. Data about the bacterial profile and current antimicrobial resistance status of Otitis Media in the region is scarce. Objective of this study determine the causative organisms of Otitis Media and antimicrobial  susceptibility patterns among children in a Primary Health Care Corporation (PPHC) centers, Qatar.Methods: A cross-sectional study was conducted in PPHC from Jan 2016 to Dec 2017. A total of 181 patients with Otitis Media presented to PHCC centers were enrolled in the study. Socio-demographic and clinical data were documented and analysed.Results: A total of 181 children, an almost equal number of male (49.7%) and female (50.3%) participants were involved in the study. 51.4% of participants were ≤5 years of age. Bacteria isolated were: Pseudomonas Aeruginosa (27.6%), Hemophilus Influenza (13.3%), Staphylococcus Aureus (11.6%), Methicillin-Resistant Staphylococcus Aureus (MSRA) (11.0%), Streptococcus Pyogenes (10.5%), Streptococcus Pneumonia (6.6%), Moraxella Catarrhalis (2.2%), Klebsiella Pneumonia (0.6%). The sensitivity of Pseudomonas Aeruginosa was (100%) to cotrimoxazole, vancomycin, and piperacillin, (96%) to cefepime and gentamicin, and was (88.2%) to ciprofloxacin. MRSA sensitivity was (100%) to vancomycin, rifampicin, and teicoplanin, (89%) to clindamycin.Conclusion: Pseudomonas Aeruginosa was the most frequent isolated bacteria. An overall antimicrobial resistance pattern seen in bacteria isolates ranges from 0% to 66.7%. The antimicrobial-resistant rate was observed for Ampicillin, Augmentin, and cefuroxime whereas ciprofloxacin, cefepime, chloramphenicol, cotrimoxazole, gentamicin, vancomycin, and amikacin were found effective for the isolated resistant bacteria.


Author(s):  
Mohammad Farooq Bhutta ◽  
Ashfaq Hussain ◽  
Salman Baig ◽  
Asad Ullah ◽  
Sarwath Fatimee ◽  
...  

Aim: To find out the frequency of different bacterial species and their antibiogram among the patients of chronic suppurative otitis media. Study Design: Descriptivecross-sectional. Place and Duration of Study: The study was conducted at the ENT department of Bahawal Victoria hospital between July to December 2020. Methodology: About 50 patients presented with unilateral or bilateral ear discharge and diagnosed as a case of chronic suppurative otitis media were included in the study. The disk diffusion method was used on Mueller-Hinton Agar to detect antimicrobial susceptibility. Multipledrugs were tested for checking antimicrobial susceptibility. The data were analyzed by using Statistical Package for the Social Sciences (SPSS) version 20. Results: The mean age of the study participants was 13.89 ± 12.37 years. The majority of participants were froma younger age group i.e. less than10 years of age with female predominance. It had been found that the majority of cases (51%) were having Staphylococcus aureus infestation followed by Pseudomonas aeruginosa, Escherichia coli and very few with Proteus mirabilis. On the other hand, multiple drugs were tested to find out the antimicrobial susceptibility among the cases of chronic otitis media and the results reported that all the bacterial species were susceptible to Ceftriaxone while the other antibiotics were havinga variable response for different strains of bacteria. Conclusion: Results concluded that the Staphylococcus aureus was the most common organism followed by Pseudomonas aeruginosa, Escherichia coli and very few with Proteus mirabilis. It is important to identify the pathogenic organism and its sensitivity pattern before prescribing any antibiotic.


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