In-vitro bioaccessibility of spray dried refined kenaf (Hibiscus cannabinus) seed oil applied in coffee drink

In the present study, spray-dried whey protein concentrate–iron (WPC–Fe) complex was prepared using a laboratory-scale spray drier under the optimized conditions of inlet temperature 180 °C, flow rate 2.66 mL/min and total solids 15 % with the objective to make iron compatible with food products. In order to remove the free iron from the bound iron, standardised method involving centrifugation and ultrafiltration was employed. Further, the retentate was subjected to spray drying to produce WPC–Fe complex. Milk fortified with WPC–Fe complex (γ(iron)=15 mg/L) showed non-significant difference in heat stability, rennet coagulation time, colour estimation, curd tension, viscosity and sensory attributes as compared to control milk. In vitro bioaccessibility of iron and induction period of the fat from milk fortified with WPC–Fe complex were found to be slightly higher (p<0.05) than that of milk fortified with iron alone. Therefore, milk can be fortified with up to 15 mg/L iron in the form of WPC–Fe complex without significantly affecting its physicochemical properties.

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Effect of chemical refining on the quality of kenaf (hibiscus cannabinus) seed oil

Industrial Crops and Products ◽

10.1016/j.indcrop.2016.05.002 ◽

2016 ◽

Vol 89 ◽

pp. 59-65 ◽

Cited By ~ 38

Author(s):

Sook-Chin Chew ◽

Chin-Ping Tan ◽

Kamariah Long ◽

Kar-Lin Nyam

Keyword(s):

Seed Oil ◽

Hibiscus Cannabinus ◽

Chemical Refining ◽

Hibiscus Cannabinus Seed Oil

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Encapsulation Effect on the In Vitro Bioaccessibility of Sacha Inchi Oil (Plukenetia volubilis L.) by Soft Capsules Composed of Gelatin and Cactus Mucilage Biopolymers

Polymers ◽

10.3390/polym12091995 ◽

2020 ◽

Vol 12 (9) ◽

pp. 1995

Author(s):

María Carolina Otálora ◽

Robinson Camelo ◽

Andrea Wilches-Torres ◽

Agobardo Cárdenas-Chaparro ◽

Jovanny A. Gómez Castaño

Keyword(s):

Gas Chromatography ◽

Seed Oil ◽

Water Solubility ◽

Oxidation Products ◽

Gas Chromatography Mass Spectrometry ◽

In Vitro Bioaccessibility ◽

Plukenetia Volubilis ◽

Sacha Inchi ◽

Cactus Mucilage

Sacha inchi (Plukenetia volubilis L.) seed oil is a rich source of polyunsaturated fatty acids (PUFAs) that are beneficial for human health, whose nutritional efficacy is limited because of its low water solubility and labile bioaccessibility (compositional integrity). In this work, the encapsulation effect, using blended softgels of gelatin (G) and cactus mucilage (CM) biopolymers, on the PUFAs’ bioaccessibility of P. volubilis seed oil was evaluated during in vitro simulated digestive processes (mouth, gastric, and intestinal). Gas chromatography–mass spectrometry (GC–MS) and gas chromatography with a flame ionization detector (GC–FID) were used for determining the chemical composition of P. volubilis seed oil both before and after in vitro digestion. The most abundant compounds in the undigested samples were α-linolenic, linoleic, and oleic acids with 59.23, 33.46, and 0.57 (g/100 g), respectively. The bioaccessibility of α-linolenic, linoleic, and oleic acid was found to be 1.70%, 1.46%, and 35.8%, respectively, along with the presence of some oxidation products. G/CM soft capsules are capable of limiting the in vitro bioaccessibility of PUFAs because of the low mucilage ratio in their matrix, which influences the enzymatic hydrolysis of gelatin, thus increasing the release of the polyunsaturated content during the simulated digestion.

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Comparison of Supercritical Fluid and Hexane Extraction Methods in Extracting Kenaf (Hibiscus cannabinus) Seed Oil Lipids

Journal of the American Oil Chemists Society ◽

10.1007/s11746-010-1754-z ◽

2011 ◽

Vol 88 (7) ◽

pp. 931-935 ◽

Cited By ~ 25

Author(s):

Abdalbasit Adam Mariod ◽

Bertrand Matthäus ◽

Maznah Ismail

Keyword(s):

Supercritical Fluid ◽

Seed Oil ◽

Extraction Methods ◽

Hibiscus Cannabinus ◽

Hexane Extraction ◽

Hibiscus Cannabinus Seed Oil

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In vitro antioxidant potential of spray-dried Psidium guajava L. leaves extracts

Planta Medica ◽

10.1055/s-0033-1352418 ◽

2013 ◽

Vol 79 (13) ◽

Author(s):

MR Fernandes ◽

CR Souza ◽

ML Martinez ◽

WP Oliveira

Keyword(s):

Psidium Guajava ◽

Antioxidant Potential ◽

Spray Dried

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1538 Influence of molybdenum concentration, pH, and transit time on the in vitro bioaccessibility of sulfur

Journal of Animal Science ◽

10.2527/jam2016-1538 ◽

2016 ◽

Vol 94 (suppl_5) ◽

pp. 747-747

Author(s):

J. Hawley ◽

E. B. Kegley

Keyword(s):

Transit Time ◽

In Vitro Bioaccessibility ◽

Molybdenum Concentration

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Preliminary Phytochemical Analysis and Anti-Bacterial Activity of Cucurbita Maxima Seed Oil – An In Vitro Study

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp1.405 ◽

2020 ◽

Vol 12 (sp1) ◽

Keyword(s):

Seed Oil ◽

In Vitro Study ◽

Bacterial Activity ◽

Vitro Study ◽

Phytochemical Analysis ◽

Cucurbita Maxima

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In Vitro Estimation of Photo-Protective Potential of Pomegranate Seed Oil and Development of a Nanoformulation

Current Nutrition & Food Science ◽

10.2174/1573401314666180223134235 ◽

2019 ◽

Vol 15 (1) ◽

pp. 87-102 ◽

Cited By ~ 2

Author(s):

Surbhi Dhawan ◽

Sanju Nanda

Keyword(s):

Particle Size ◽

Zeta Potential ◽

Seed Oil ◽

Topical Delivery ◽

Inflammatory Activity ◽

Pomegranate Seed Oil ◽

Protective Potential ◽

Release Studies ◽

Pomegranate Seed

Background: Since ancient times, people have been using natural resources for photoprotection purposes. One such highly recognised natural agent is pomegranate seed oil, considered as wonder oil owing to the presence of several beneficial phytoconstituents. Objective: The study aimed to establish the photoprotective potential of pomegranate seed oil through various in vitro and biochemical studies along with the formation of nanoemulsion, an efficient topical delivery system for the oil. Method: Photo-protective potential of the oil was estimated by determining in vitro antioxidant and anti-inflammatory activity, total phenolic content, anti elastase, antihyaluronidase and anticollagenase activities of the oil. Ultrasonication method was used to formulate nanoemulsions. The optimisation was done following the central composite design. The characterisation was done by particle size analysis, zeta potential, polydispersity index, pH, viscosity, stability testing and transmission electron microscopy. The optimised nanoemulsion was loaded into a gel base for topical application and further release studies were carried out. Results: The IC50 values of anti-elastase, anti-collagenase and anti-hyaluronidase were found to be 309 mg/ml, 4 mg/ml and 95 mg/ml respectively. The results of anti-oxidant and anti-inflammatory activity were also significant, which thereby established the photo-protective potential of the oil. The optimum batch 2 had particle size 83.90 nm, 0.237 PDI and -5.37 mV zeta potential. The morphology was confirmed by TEM. Batch 2 was incorporated into a gel base and release studies showed 74.12 % release within 7 hours. Conclusion: Pomegranate seed oil possesses a potential photo-protective ability. Nanoemulsions proved to be a promising carrier for the topical delivery of the oil.

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In Vitro Bioaccessibility of Bioactive Compounds from Citrus Pomaces and Orange Pomace Biscuits

Molecules ◽

10.3390/molecules26123480 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3480

Author(s):

Adriana Maite Fernández-Fernández ◽

Eduardo Dellacassa ◽

Tiziana Nardin ◽

Roberto Larcher ◽

Adriana Gámbaro ◽

...

Keyword(s):

Bioactive Compounds ◽

Total Phenol Content ◽

No Production ◽

Food Ingredient ◽

Potential Health ◽

Raw264.7 Macrophages ◽

In Vitro Bioaccessibility ◽

Health Promoting ◽

Orange Pomace

The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6–11.0 mg GAE/g digest, 65.5–97.1 µmol Trolox Equivalents (TE)/g digest, and 135.5–214.8 µmol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction (p < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5–25 µg/mL of the digests], anti-inflammatory [significant reduction (p < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC50 3.97–11.42 mg/mL and 58.04–105.68 mg/mL for α-glucosidase and α-amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims “no-added sugars” and “source of fiber”, as well as those with good sensory quality (6.9–6.7, scale 1–9) and potential health promoting properties, were obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.

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