scholarly journals Characterization of multiple metal resistant Bacillus licheniformis and its potential use in arsenic contaminated industrial wastewater

2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Shahid Sher ◽  
Sikander Sultan ◽  
Abdul Rehman
Keyword(s):  
Bacillus Licheniformis ◽  
Toxic Metal ◽  
Growth Conditions ◽  
Oxidation Potential ◽  
Optimum Growth ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence ◽  
Optimum Growth Temperature ◽  
Potential Use

AbstractIn the present study, the arsenic bioremediation ability of Bacillus licheniformis (dubbed as A6) was determined. The strain was isolated from metal polluted wastewater and was identified on the basis of 16S rRNA sequence homology with accession number of KX 785,171. The bacterium showed resistance against multiple toxic heavy metals, and MIC against arsenic was 3000 µg/ml. Resistance of the bacterium against other toxic metal ions was 3000 µg/ml (Cr), 50 µg/ml (Hg), 1000 µg/ml (Mn), 4000 µg/ml (Se), 500 µg/ml (Pb), 100 µg/ml (Co), 70 µg/ml (Cd) and 100 µg/ml (Zn). The optimum growth temperature was 37 °C while pH was 7. The strain also showed resistance against commonly used antibiotics except ceftriaxone 30 µg and amoxicillin with clavulanic acid (2:1) 3 µg. B. licheniformis could oxidize arsenite into arsenate 86 and 98% after 48 and 96 h from the medium at optimum growth conditions. Due to its high oxidation potential, B. licheniformis can be used in the biological treatment of wastewater containing arsenic.

scholarly journals Study of an arsenic metabolizing bacteria from arsenic contaminated soil of Chandpur district, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 57-65
Author(s):  
Roksana Khanam ◽  
Ripa Moni ◽  
Md Zahidul Islam ◽  
Md Morsaline Billah ◽  
Umme Salma Zohora ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Bacterial Species ◽  
Toxic Metal ◽  
Extracellular Enzyme ◽  
Bacterial Isolates ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence ◽  
Yeast Extract Mannitol ◽  
16S Rrna Sequence Analysis

Arsenic is a toxic metal found as inorganic oxyanion arsenate As(V) and arsenite As (III) species. The disposal of toxic heavy metals such as arsenic poses high risk to environment. The present study was undertaken to isolate arsenic-metabolizing bacteria from arsenic contaminated soil of Chandpur district, which is one of the most arsenic contaminated area in Bangladesh and later these bacteria were screened for their ability to metabolize arsenate. Out of ninety eight isolates, ten were found to be capable of metabolizing arsenic in Yeast Extract Mannitol (YEM) medium containing 2 mM arsenate at 37ºC. One of the bacterial isolates designated as I-25 was found to produce an extracellular enzyme which can reduce As(V) into As(III) and able to grow in presence of up to 500 mM arsenate. Subsequent molecular identification of this enzyme producing bacterial isolate using 16s rRNA sequence analysis was correlated with previously identified isolate as Bacillus aryabhatti. Further characterization of the enzyme showed that optimum pH of the extracellular enzyme by the bacterial species was 7 and optimum temperature for the enzyme activity was 60ºC. The bacterial isolates can be exploited for the study of possible bioremediation of arsenic contamination. Jahangirnagar University J. Biol. Sci. 8(1): 57-65, 2019 (June)

Screening and Characterization of a Bacteria Strain L0 for Degradation of Cypermethrin

2012 ◽  
Vol 209-211 ◽  
pp. 1158-1161
Author(s):  
Bin Hui Jiang ◽  
Li Jiang ◽  
Zhi Ming Li ◽  
Kai Kang ◽  
Ruo Nan Li
Keyword(s):  
Gas Chromatography ◽  
Contaminated Soils ◽  
Growth Conditions ◽  
Polluted Soil ◽  
Aerobic Degradation ◽  
Optimum Growth ◽  
Initial Screening ◽  
High Effect ◽  
Minimal Media

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution.

Isolation and Characterization of a Sulfanilic Acid Degrading Bacterial Strain

2011 ◽  
Vol 148-149 ◽  
pp. 46-49 ◽  
Author(s):  
Yan Qing Yang ◽  
Chu Wu ◽  
Lu Yun Wang ◽  
Ling Yang
Keyword(s):  
Bacterial Strain ◽  
Sulfanilic Acid ◽  
Optimum Growth ◽  
Biochemical Characteristics ◽  
Optimum Growth Temperature ◽  
Physiological And Biochemical Characteristics ◽  
Isolation And Characterization ◽  
Physiological And Biochemical ◽  
Sulfonated Azo Dyes

Sulfanilic acid is a representative intermediate of some sulfonated azo dyes. A bacterial strain isolated from the river in Wenzhou, could utilize sulfanilic acid as the solo carbon source and energy source. Based on its morphological, physiological and biochemical characteristics as well as 16SrRNA sequences the bacterial strain was identified as Ochrobactrum anthrop. Effective biodegradation of sulfanilic acid occurred at pH ranging from 6 to 8. The optimum growth temperature and pH for the bacterial strain to utilize sulfanilic acid are 30°C and 7.0 respectively. Its most favorable sulfanilic acid concentration is 300mg/L.

Growth Responses in Sorghum and Wheat Induced by Glyphosate

Weed Science ◽  
1977 ◽  
Vol 25 (3) ◽  
pp. 238-240 ◽  
Author(s):  
J.R. Baur ◽  
R.W. Bovey ◽  
J.A. Veech
Keyword(s):  
Foliar Application ◽  
Normal Growth ◽  
Growth Zone ◽  
Growth Conditions ◽  
Optimum Growth ◽  
Growth Responses ◽  
Fresh Weight ◽  
Optimum Growth Temperature ◽  
Chamber Studies ◽  
Growth Chamber Studies

Foliar application of 2.8 μg/plant of glyphosate [N-(phosphonomethyl)glycine] to greenhouse grown sorghum [Sorghum bicolor (L.) Moench ‘Tophand’] seedlings resulted in increased fresh weight. As glyphosate levels were increased to 11.2 μg/plant, diameter of the basal growth zone increased while fresh weight decreased. In growth chamber studies with sorghum and wheat [Triticum aestivum (L.) ‘Era’] seedlings, glyphosate caused the greatest reduction in fresh weight at the optimum growth temperatures for both species. Glyphosate inhibited normal production of basal buds in wheat at the optimum growth temperature and stimulated bud production at temperatures above the optimum. Under normal growth conditions, basal buds in sorghum do not develop; however, application of glyphosate stimulated basal bud development under normal and above-normal temperature conditions. Histochemical analysis of malate dehydrogenase activity in apical meristem tissue of treated sorghum seedlings indicated that growth of the apex was normal and viable.

scholarly journals Fibrinolysin production by Alcaligenes faecalis strain 26 isolated from environment

2019 ◽  
Author(s):  
Zahra Nikkhoy ◽  
Hossein Motamedi
Keyword(s):  
Environmental Samples ◽  
Blood Clot ◽  
Good Alternative ◽  
Growth Conditions ◽  
Alcaligenes Faecalis ◽  
Fibrin Clot ◽  
Clot Lysis ◽  
Optimum Growth ◽  
Optimum Growth Temperature ◽  
The Stability

Background and Objectives: Fibrinolytic drugs are commonly used for fibrin clot lysis but due to their inappropriate side effects, as well as their high costs, using fibrinolytic enzymes has been paid attention. Bacterial sources of this enzyme are a good alternative for this purpose. The aim was fibrinolysin production through screening of fibrinolysin producing bacteria from environmental samples. Materials and Methods: Bacterial isolation was performed from different environmental samples and was screened based on sheep blood clot digestion and culture on plasma plate. The most potent isolate was optimized for different growth param- eters including temperature, pH and fibrinolysin production at optimum growth conditions. The stability of produced enzyme at various temperatures and pH and treatment with MgSO , NiSO , SDS and EDTA was then investigated. Finally this isolate 4 4 was identified based on the 16S rRNA sequencing. Results: As a result, from 79 different isolates, the most potent fibrinolysin producer was identified as Alcaligenes faecalis strain 26. This isolate produced 12 mm halo zone on plasma plate. Its optimum growth temperature and pH was 43°C and 7, respectively. The produced enzyme had the best stability at pH 7 and was also active up to 60°C. The fibrinolytic activity of this isolate was reduced following treatment with MgSO , NiSO and also with protease inhibitors, such as SDS and EDTA. 4 4 Conclusion: Based on the obtained results it can be suggested that Alcaligenes faecalis strain 26 has appropriate efficiency for fibrinolysin production that can be used in food industry and medicine.

scholarly journals Assessment of cadmium tolerance and biosorptive potential of Bacillus Cereus GCFSD01 isolated from cadmium contaminated soil

2020 ◽  
Author(s):  
S. Muzammil ◽  
M. H. Siddique ◽  
F. Mureed ◽  
R. Andleeb ◽  
F. Jabeen ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Strain ◽  
Bacterial Isolate ◽  
Metal Tolerance ◽  
Growth Conditions ◽  
Food Chains ◽  
Optimum Growth ◽  
Mic Value ◽  
Cadmium Contaminated Soil

Abstract Continuous occurrence of heavy metals is a major cause of environmental pollution due to its toxic effects. At minimum concentrations, these metals are highly reactive and can gather in the food chains and food web, causing major dangers to public health concerns. Soil samples were collected from Paharang drain, Faisalabad. Cadmium tolerant bacteria were isolated and evaluated for their MIC against Cd. The isolated bacterial strain GCFSD01 showed MIC value upto 30 mM/L. The bacterial strain with the highest resistance against Cd was selected for further study. Molecular characterization of bacterial isolate GCFSD01 was performed by 16S rRNA which confirmed it as Bacillus cereus. Optimum growth conditions of bacterial strain were also evaluated. Strain GCFSD01 showed optimum growth at pH 7 and 37 °C temperature. Our result revealed that B. cereus strain GCFSD01 reduced 61.3% Cd after 48 hrs. Multiple metal tolerance and Cd reduction by B. cereus indicate its potential for further use for decontamination of polluted soil.

scholarly journals Isolation and screening of chromium resistant bacteria from industrial waste for bioremediation purposes

2023 ◽  
Vol 83 ◽  
Author(s):  
Kalsoom ◽  
Afshan Batool ◽  
Ghufranud Din ◽  
Salah Ud Din ◽  
Johar Jamil ◽  
...  
Keyword(s):  
Industrial Waste ◽  
Heavy Metal Contamination ◽  
Industrial Effluent ◽  
Toxic Metal ◽  
Maximum Growth ◽  
Major Constituent ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence

Abstract Chromium (VI) a highly toxic metal, a major constituent of industrial waste. It is continuously release in soil and water, causes environmental and health related issues, which is increasing public concern in developing countries like Pakistan. The basic aim of this study was isolation and screening of chromium resistant bacteria from industrial waste collected from Korangi and Lyari, Karachi (24˚52ʹ46.0ʺN 66˚59ʹ25.7ʺE and 24˚48ʹ37.5ʺN 67˚06ʹ52.6ʺE). Among total of 53 isolated strains, seven bacterial strains were selected through selective enrichment and identified on the basis of morphological and biochemical characteristics. These strains were designated as S11, S13, S17, S18, S30, S35 and S48, resistance was determined against varying concentrations of chromium (100-1500 mg/l). Two bacterial strains S35 and S48 showed maximum resistance to chromium (1600 mg/l). Bacterial strains S35 and S48 were identified through 16S rRNA sequence and showed 99% similarity to Bacillus paranthracis and Bacillus paramycoides. Furthermore, growth condition including temperature and pH were optimized for both bacterial strains, showed maximum growth at temperature 30ºC and at optimum pH 7.5 and 6.5 respectively. It is concluded that indigenous bacterial strains isolated from metal contaminated industrial effluent use their innate ability to transform toxic heavy metals to less or nontoxic form and can offer an effective tool for monitoring heavy metal contamination in the environment.

scholarly journals Naturally Occurring Ecdysteroids in Triticum aestivum L. and Evaluation of Fenarimol as a Potential Inhibitor of Their Biosynthesis in Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2855
Author(s):  
Anna Janeczko ◽  
Jana Oklestkova ◽  
Danuše Tarkowská ◽  
Barbara Drygaś
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Animal Kingdom ◽  
Experimental Conditions ◽  
P450 Monooxygenase ◽  
Naturally Occurring ◽  
Regulatory Effects ◽  
Potential Use

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensitive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the biosynthesis and physiological function of these substances in plants.

Bark characterization of a commercial Eucalyptus urophylla hybrid clone in view of its potential use as a biorefinery raw material

2021 ◽  
Author(s):  
Caroline J. Sartori ◽  
Graciene S. Mota ◽  
Fábio Akira Mori ◽  
Isabel Miranda ◽  
Teresa Quilhó ◽  
...  
Keyword(s):  
Raw Material ◽  
Hybrid Clone ◽  
Eucalyptus Urophylla ◽  
Potential Use
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