Effect of copper nanoparticles on physico-chemical properties of chitosan and gelatin-based scaffold developed for skin tissue engineering application

Abstract Sodium alginate (SA) approved its high potential in tissue engineering and regenerative medicine. One of the main weaknesses of this polysaccharide is its low spinnability which nanofiber based scaffolds are the interest of scientists in biomedical engineering. The main aim of this study was to improve the spinnability of SA in combination with polyvinyl alcohol (PVA). It was also tried to optimize the main parameters in electrospinning of the optimized SA;PVA ratio including voltage, flow rate, and working space. To aim this, Response surface methodology under central composite design was employed to design the experiments scientifically. The final nanofiber scaffolds were studied using scanning electron microscopy, Fourier transform infrared spectroscopy, degradability, swelling, tensile strength, porosity, nanofiber diameter, contact angle, and cytotoxicity. Based on the results, the best ratio for SA:PVA was 1:6.5. The solution with this concentration was spinnable in various values for the process parameters. The fabricated scaffolds under these conditions revealed good physical, chemical, mechanical, and biological features. L929 cell lines revealed high viability during 48 h of culture. The results revealed the uniform and homogeneous nanofibers with the regular size distribution (166 nm) were obtained at 30 kV, 0.55 µl/h, and 12.5 cm. To sum up, the optimized ratio under the reported conditions can be a good biologically compatible candidate for skin tissue engineering.

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Skin Tissue Engineering: Application of Adipose-Derived Stem Cells

BioMed Research International ◽

10.1155/2017/9747010 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 28

Author(s):

Agnes S. Klar ◽

Jakub Zimoch ◽

Thomas Biedermann

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Engineering Application ◽

Stromal Vascular Fraction ◽

Skin Tissue ◽

Tissue Engineering Application ◽

Adipose Derived Stem Cells ◽

Skin Tissue Engineering ◽

Stem Cell Source

Perception of the adipose tissue has changed dramatically over the last few decades. Identification of adipose-derived stem cells (ASCs) ultimately transformed paradigm of this tissue from a passive energy depot into a promising stem cell source with properties of self-renewal and multipotential differentiation. As compared to bone marrow-derived stem cells (BMSCs), ASCs are more easily accessible and their isolation yields higher amount of stem cells. Therefore, the ASCs are of high interest for stem cell-based therapies and skin tissue engineering. Currently, freshly isolated stromal vascular fraction (SVF), which may be used directly without any expansion, was also assessed to be highly effective in treating skin radiation injuries, burns, or nonhealing wounds such as diabetic ulcers. In this paper, we review the characteristics of SVF and ASCs and the efficacy of their treatment for skin injuries and disorders.

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Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications

RSC Advances ◽

10.1039/c5ra19529b ◽

2016 ◽

Vol 6 (10) ◽

pp. 7914-7922 ◽

Cited By ~ 41

Author(s):

Giriprasath Ramanathan ◽

Sivakumar Singaravelu ◽

M. D. Raja ◽

Naveen Nagiah ◽

P. Padmapriya ◽

...

Keyword(s):

Tissue Engineering ◽

Engineering Application ◽

Nanofibrous Scaffold ◽

Skin Tissue ◽

Tissue Engineering Application ◽

Hydroxybutyric Acid ◽

Skin Tissue Engineering ◽

Promising Tool ◽

Good Biocompatibility

The collagen coated nanofibrous scaffold mimics the function of the extra cellular matrix with good biocompatibility, cell adhesion, cell proliferation and aids to provide as a promising tool in skin tissue engineering application.

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Hybrid Methacrylated Gelatin and Hyaluronic Acid Hydrogel Scaffolds. Preparation and Systematic Characterization for Prospective Tissue Engineering Applications

International Journal of Molecular Sciences ◽

10.3390/ijms22136758 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6758

Author(s):

B. Velasco-Rodriguez ◽

T. Diaz-Vidal ◽

L. C. Rosales-Rivera ◽

C. A. García-González ◽

C. Alvarez-Lorenzo ◽

...

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Engineering Application ◽

Chemical Properties ◽

Tissue Engineering Application ◽

Hydrogel Scaffolds ◽

Hybrid Hydrogels ◽

Physico Chemical ◽

Scaffold Structure ◽

The Relationship

Hyaluronic acid (HA) and gelatin (Gel) are major components of the extracellular matrix of different tissues, and thus are largely appealing for the construction of hybrid hydrogels to combine the favorable characteristics of each biopolymer, such as the gel adhesiveness of Gel and the better mechanical strength of HA, respectively. However, despite previous studies conducted so far, the relationship between composition and scaffold structure and physico-chemical properties has not been completely and systematically established. In this work, pure and hybrid hydrogels of methacroyl-modified HA (HAMA) and Gel (GelMA) were prepared by UV photopolymerization and an extensive characterization was done to elucidate such correlations. Methacrylation degrees of ca. 40% and 11% for GelMA and HAMA, respectively, were obtained, which allows to improve the hydrogels’ mechanical properties. Hybrid GelMA/HAMA hydrogels were stiffer, with elastic modulus up to ca. 30 kPa, and porous (up to 91%) compared with pure GelMA ones at similar GelMA concentrations thanks to the interaction between HAMA and GelMA chains in the polymeric matrix. The progressive presence of HAMA gave rise to scaffolds with more disorganized, stiffer, and less porous structures owing to the net increase of mass in the hydrogel compositions. HAMA also made hybrid hydrogels more swellable and resistant to collagenase biodegradation. Hence, the suitable choice of polymeric composition allows to regulate the hydrogels´ physical properties to look for the most optimal characteristics required for the intended tissue engineering application.

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Adhesion and proliferation properties of type I collagen‐derived peptide for possible use in skin tissue engineering application

Cell Biology International ◽

10.1002/cbin.11737 ◽

2021 ◽

Author(s):

K Sivaraman ◽

K Muthukumar ◽

C Shanthi

Keyword(s):

Tissue Engineering ◽

Type I Collagen ◽

Engineering Application ◽

Skin Tissue ◽

Tissue Engineering Application ◽

Type I ◽

Skin Tissue Engineering

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Hybrid PCL/chitosan-PEO nanofibrous scaffolds incorporated with A. euchroma extract for skin tissue engineering application

Carbohydrate Polymers ◽

10.1016/j.carbpol.2021.118926 ◽

2021 ◽

pp. 118926

Author(s):

Fatemeh Asghari ◽

Davood Rabiei Faradonbeh ◽

Ziba Veisi Malekshahi ◽

Houra Nekounam ◽

Behnaz Ghaemi ◽

...

Keyword(s):

Tissue Engineering ◽

Engineering Application ◽

Skin Tissue ◽

Tissue Engineering Application ◽

Skin Tissue Engineering ◽

Nanofibrous Scaffolds

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Poly(3-hydroxybutyrate-co-valerate)/poly(3-thiophene ethyl acetate) blends as a electroactive biomaterial substrate for tissue engineering application

RSC Advances ◽

10.1039/c5ra26747a ◽

2016 ◽

Vol 6 (30) ◽

pp. 25330-25338 ◽

Cited By ~ 10

Author(s):

M. S. Recco ◽

A. C. Floriano ◽

D. B. Tada ◽

A. P. Lemes ◽

R. Lang ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Ethyl Acetate ◽

Mammalian Cell ◽

Engineering Application ◽

Chemical Properties ◽

Tissue Engineering Application ◽

Good Adhesion ◽

Physical Chemical ◽

Low Cytotoxicity

Polyblend films based on poly(3-hydroxybutirate-co-valerate) and poly(3-thiophene ethyl acetate) – PHBV/PTAcEt showed low cytotoxicity, good adhesion and mammalian cell proliferation. The physical–chemical properties were explored.

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Development of a novel reinforced film based on Gellan gum/Cellulose nanofiber/Soy protein for skin tissue engineering application

New Journal of Chemistry ◽

10.1039/d1nj02623b ◽

2021 ◽

Author(s):

Zahra Mohebian ◽

Leila Yavari Maroufi ◽

Marjan Ghorbani

Keyword(s):

Tissue Engineering ◽

Soy Protein ◽

Nanocomposite Film ◽

Composite Films ◽

Engineering Application ◽

Gellan Gum ◽

Cellulose Nanofiber ◽

Tissue Engineering Application ◽

Skin Tissue Engineering ◽

Potential Applications

Recently, the importance of biocompatible nanocomposite film with suitable properties has been further attracted for potential applications in the biomedical area. In this study, composite films of Gellan gum/Soy Protein/Cellulose...

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Crosslinking Collagen Constructs: Achieving Cellular Selectivity Through Modifications of Physical and Chemical Properties

Applied Sciences ◽

10.3390/app10196911 ◽

2020 ◽

Vol 10 (19) ◽

pp. 6911

Author(s):

Malavika Nair ◽

Serena M. Best ◽

Ruth E. Cameron

Keyword(s):

Tissue Engineering ◽

Cell Viability ◽

Biomedical Applications ◽

Engineering Application ◽

Chemical Properties ◽

Tissue Engineering Application ◽

Physical And Chemical Properties ◽

Crosslinking Process ◽

Physical And Chemical ◽

And Chemical Properties

Collagen-based constructs have emerged in recent years as ideal candidates for tissue engineering implants. For many biomedical applications, collagen is crosslinked in order to improve the strength, stiffness and stability of the construct. However, the crosslinking process may also result in unintended changes to cell viability, adhesion or proliferation on the treated structures. This review provides a brief overview of some of both the most commonly used and novel crosslinkers used with collagen, and suggests a framework by which crosslinking methods can be compared and selected for a given tissue engineering application.

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