Chemoprophylaxis under sporozoites-lumefantrine (CPS-LMF) immunization induce protective immune responses against Plasmodium yoelii sporozoites infection in mice

3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Arif Jamal Siddiqui ◽  
Jyoti Bhardwaj ◽  
Walid Sabri Hamadou ◽  
Manish Goyal ◽  
Syed Amir Ashraf ◽  
...  
Keyword(s):  
Immune Responses ◽  
Plasmodium Yoelii

scholarly journals The E3 ubiquitin ligase MARCH1 regulates antimalaria immunity through interferon signaling and T cell activation

2020 ◽  
pp. 202004332 ◽  
Author(s):  
Jian Wu ◽  
Lu Xia ◽  
Xiangyu Yao ◽  
Xiao Yu ◽  
Keyla C. Tumas ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
T Cell Activation ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Plasmodium Yoelii ◽  
Cell Populations ◽  
Eqtl Analysis ◽  
Type I ◽  
Ifn Γ

Malaria infection induces complex and diverse immune responses. To elucidate the mechanisms underlying host–parasite interaction, we performed a genetic screen during early (24 h) Plasmodium yoelii infection in mice and identified a large number of interacting host and parasite genes/loci after transspecies expression quantitative trait locus (Ts-eQTL) analysis. We next investigated a host E3 ubiquitin ligase gene (March1) that was clustered with interferon (IFN)-stimulated genes (ISGs) based on the similarity of the genome-wide pattern of logarithm of the odds (LOD) scores (GPLS). March1 inhibits MAVS/STING/TRIF-induced type I IFN (IFN-I) signaling in vitro and in vivo. However, in malaria-infected hosts, deficiency of March1 reduces IFN-I production by activating inhibitors such as SOCS1, USP18, and TRIM24 and by altering immune cell populations. March1 deficiency increases CD86+DC (dendritic cell) populations and levels of IFN-γ and interleukin 10 (IL-10) at day 4 post infection, leading to improved host survival. T cell depletion reduces IFN-γ level and reverse the protective effects of March1 deficiency, which can also be achieved by antibody neutralization of IFN-γ. This study reveals functions of MARCH1 (membrane-associated ring-CH–type finger 1) in innate immune responses and provides potential avenues for activating antimalaria immunity and enhancing vaccine efficacy.

scholarly journals Mycobacterium-Induced Potentiation of Type 1 Immune Responses and Protection against Malaria Are Host Specific

2005 ◽  
Vol 73 (12) ◽  
pp. 8369-8380 ◽  
Author(s):  
Kathleen R. Page ◽  
Anne E. Jedlicka ◽  
Benjamin Fakheri ◽  
Gregory S. Noland ◽  
Anup K. Kesavan ◽  
...  
Keyword(s):  
Immune Responses ◽  
Plasmodium Yoelii ◽  
Short Term ◽  
Necrosis Factor Alpha ◽  
The World ◽  
Peak Parasitemia ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Necrosis Factor

ABSTRACT Malaria and tuberculosis are endemic in many regions of the world, and coinfection with the two pathogens is common. In this study, we examined the effects of long- and short-term infection with Mycobacterium tuberculosis on the course of a lethal form of murine malaria in resistant (C57BL/6) and susceptible (BALB/c) mice. C57BL/6 mice coinfected with M. tuberculosis CDC1551 and Plasmodium yoelii 17XL had a lower peak parasitemia and increased survival compared to mice infected with P. yoelii 17XL alone. Splenic microarray analysis demonstrated potentiation of type 1 immune responses in coinfected C57BL/6 mice, which was especially prominent 5 days after infection with P. yoelii 17XL. Splenocytes from coinfected C57BL/6 mice produced higher levels of gamma interferon (IFN-γ) and tumor necrosis factor alpha than splenocytes from mice infected with either pathogen alone. Interestingly, mycobacterium-induced protection against lethal P. yoelii is mouse strain specific. BALB/c mice were significantly more susceptible than C57BL/6 mice to infection with P. yoelii 17XL and were not protected against lethal malaria by coinfection with M. tuberculosis. In addition, M. tuberculosis did not augment IFN-γ responses in BALB/c mice subsequently infected with P. yoelii 17XL. These data indicate that M. tuberculosis-induced potentiation of type 1 immune responses is associated with protection against lethal murine malaria.

scholarly journals Induction of Multifunctional Broadly Reactive T Cell Responses by a Plasmodium vivax Circumsporozoite Protein Recombinant Chimera

2015 ◽  
Vol 83 (9) ◽  
pp. 3749-3761 ◽  
Author(s):  
Monica Cabrera-Mora ◽  
Jairo Andres Fonseca ◽  
Balwan Singh ◽  
Joseli Oliveira-Ferreira ◽  
Josué da Costa Lima-Junior ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Immune Responses ◽  
Plasmodium Vivax ◽  
Vaccine Candidate ◽  
Chimeric Protein ◽  
Plasmodium Yoelii ◽  
Circumsporozoite Protein ◽  
Soluble Form ◽  
Content Type

Plasmodium vivaxis the most widespread species ofPlasmodium, causing up to 50% of the malaria cases occurring outside sub-Saharan Africa. An effective vaccine is essential for successful control and potential eradication. A well-characterized vaccine candidate is the circumsporozoite protein (CSP). Preclinical and clinical trials have shown that both antibodies and cellular immune responses have been correlated with protection induced by immunization with CSP. On the basis of our reported approach of developing chimericPlasmodium yoeliiproteins to enhance protective efficacy, we designed PvRMC-CSP, a recombinant chimeric protein based on theP. vivaxCSP (PvCSP). In this engineered protein, regions of the PvCSP predicted to contain human T cell epitopes were genetically fused to an immunodominant B cell epitope derived from the N-terminal region I and to repeat sequences representing the two types of PvCSP repeats. The chimeric protein was expressed in soluble form with high yield. As the immune response to PvCSP has been reported to be genetically restricted in the murine model, we tested the immunogenicity of PvRMC-CSP in groups of six inbred strains of mice. PvRMC-CSP was able to induce robust antibody responses in all the mouse strains tested. Synthetic peptides representing the allelic forms of theP. vivaxCSP were also recognized to a similar extent regardless of the mouse strain. Furthermore, the immunization regimen induced high frequencies of multifunctional CD4+and CD8+PvRMC-CSP-specific T cells. The depth and breadth of the immune responses elicited suggest that immunization with PvRMC-CSP can circumvent the genetic restriction of the immune response toP. vivaxCSP. Interestingly, PvRMC-CSP was also recognized by naturally acquired antibodies from individuals living in areas where malaria is endemic. These features make PvRMC-CSP a promising vaccine candidate for further development.

scholarly journals Exosome-Based Vaccines: Pros and Cons in the World of Animal Health

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1499
Author(s):  
Sergio Montaner-Tarbes ◽  
Lorenzo Fraile ◽  
María Montoya ◽  
Hernando Del Portillo
Keyword(s):  
Food Security ◽  
Immune Responses ◽  
Animal Health ◽  
Cost Effective ◽  
Lymphocytic Choriomeningitis ◽  
Plasmodium Yoelii ◽  
Emerging Diseases ◽  
Respiratory Syndrome Virus ◽  
Effective Measure ◽  
Almost All

Due to the emergence of antibiotic resistance and new and more complex diseases that affect livestock animal health and food security, the control of epidemics has become a top priority worldwide. Vaccination represents the most important and cost-effective measure to control infectious diseases in animal health, but it represents only 23% of the total global animal health market, highlighting the need to develop new vaccines. A recent strategy in animal health vaccination is the use of extracellular vesicles (EVs), lipid bilayer nanovesicles produced by almost all living cells, including both prokaryotes and eukaryotes. EVs have been evaluated as a prominent source of viral antigens to elicit specific immune responses and to develop new vaccination platforms as viruses and EVs share biogenesis pathways. Preliminary trials with lymphocytic choriomeningitis virus infection (LCMV), porcine reproductive and respiratory syndrome virus (PRRSV), and Marek’s disease virus (MDV) have demonstrated that EVs have a role in the activation of cellular and antibody immune responses. Moreover, in parasitic diseases such as Eimeria (chickens) and Plasmodium yoelii (mice) protection has been achieved. Research into EVs is therefore opening an opportunity for new strategies to overcome old problems affecting food security, animal health, and emerging diseases. Here, we review different conventional approaches for vaccine design and compare them with examples of EV-based vaccines that have already been tested in relation to animal health.

scholarly journals Dendritic Cells Can Initiate Protective Immune Responses against Malaria

2001 ◽  
Vol 69 (8) ◽  
pp. 5173-5176 ◽  
Author(s):  
Oscar Bruña-Romero ◽  
Ana Rodriguez
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Immune Responses ◽  
Antigen Presentation ◽  
Plasmodium Yoelii

ABSTRACT An understanding of the antigen presentation mechanisms that mediate induction of protective immune responses against malaria is essential for the development of successful immunization approaches. Here we show that dendritic cells presenting Plasmodium yoelii sporozoite antigens are able to activate specific CD4+ and CD8+ T cells and initiate protective immune responses against malaria in mice.

scholarly journals Immune responses in liver and spleen against Plasmodium yoelii pre-erythrocytic stages in Swiss mice model

2020 ◽  
Vol 24 ◽  
pp. 29-41 ◽  
Author(s):  
Arif Jamal Siddiqui ◽  
Jyoti Bhardwaj ◽  
Manish Goyal ◽  
Kirtika Prakash ◽  
Mohd Adnan ◽  
...  
Keyword(s):  
Immune Responses ◽  
Plasmodium Yoelii ◽  
Mice Model ◽  
Swiss Mice
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