Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro

2018 ◽  
Vol 59 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Bożena Pawłowska ◽  
Marek Żupnik ◽  
Bożena Szewczyk-Taranek ◽  
Monika Cioć
Keyword(s):  
Photosynthetic Pigments ◽  
Light Sources ◽  
Gerbera Jamesonii ◽  
Led Light

Growth of Solanum tuberosum plantlets in vitro under LED light sources

2019 ◽  
Author(s):  
Olga Nakonechnaya ◽  
Irina Gafitskaya ◽  
Ola Grishchenko ◽  
Yuri Kulchin ◽  
Victor Bulgakov ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Light Sources ◽  
Led Light

scholarly journals Different LED Light Intensities and 6-Benzyladenine Concentrations in Relation to Shoot Development, Leaf Architecture, and Photosynthetic Pigments of Gerbera jamesonii Bolus In Vitro

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 358 ◽  
Author(s):  
Monika Cioć ◽  
Andrzej Kalisz ◽  
Marek Żupnik ◽  
Bożena Pawłowska
Keyword(s):  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Photon Flux ◽  
Gerbera Jamesonii ◽  
Leaf Architecture ◽  
Weight Content ◽  
Blade Area

A mixture of red and blue light-emitting diodes (LEDs; at a ratio of 7:3, respectively) were used to analyze the effects of different photosynthetic photon flux densities (PPFDs) (40, 80, and 120 µmol m−2 s−1 hereafter known as LED 40, 80, and 120, respectively) on the micropropagation of Gerbera jamesonii Bolus shoots. The experiment also examined the effect of 6-benzyladenine (BA) in 1, 2.5, and 5 µM concentrations in the media. Biometrical observations and analyses of leaf morphometry and photosynthetic pigment content were conducted. Shoot multiplication increased with an increasing BA concentration. A PPFD of 80 µmol m−2 s−1 and 5 µM BA is suggested as efficient for shoot propagation and economically viable. LED 120 increased the leaf blade area and its width, and circularity and elongation ratios. The intensity of light did not affect the fresh weight, which increased at higher BA concentrations (2.5 and 5 μM). The dry weight content decreased with increasing cytokinin concentration; the greatest content was observed on media with 1 µM BA under PPFD 120 µmol m−2 s−1. LED 80 increased the photosynthetic pigments content in the leaves in comparison to the standard intensity of LED 40. Increased BA concentration raises the content of chlorophyll a.

Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources

2019 ◽  
Vol 96 (4) ◽  
pp. 396-402 ◽  
Author(s):  
Lili Jiang ◽  
Ziquan Wang ◽  
Guanghui Jin ◽  
Dianqiu Lu ◽  
Xuezhan Li
Keyword(s):  
Light Emitting Diode ◽  
Light Sources ◽  
Light Emitting ◽  
Led Light ◽  
Potato Plantlets

scholarly journals Energy-Saving LED Light Affects the Efficiency of the Photosynthetic Apparatus and Carbohydrate Content in Gerbera jamesonii Bolus ex Hook. f. Axillary Shoots Multiplied In Vitro

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1035
Author(s):  
Monika Cioć ◽  
Krzysztof Tokarz ◽  
Michał Dziurka ◽  
Bożena Pawłowska
Keyword(s):  
Energy Saving ◽  
Sugar Content ◽  
Photosynthetic Apparatus ◽  
Photochemical Quenching ◽  
Gerbera Jamesonii ◽  
Axillary Shoots ◽  
Glucose Content ◽  
Ps Ii ◽  
Led Light

An energy-saving light emitting diode (LED) system allows for adjustment of light quality, which affects plant development and metabolic processes in in vitro cultures. The study investigated the content of endogenous carbohydrates and the condition of the photosynthetic apparatus of Gerbera jamesonii Bolus ex Hook. f. Our aim was to analyze the effects of different LED light qualities—100% red light (R LED), 100% blue (B LED), a mixture of red and blue (7:3) (RB LED), and a fluorescent lamp as a control (Fl)—during the multiplication of axillary shoots. After 40 days, the culture measurements were performed using a non-invasive pulse amplitude modulation (PAM) fluorimeter. Sugar content was assessed with high performance liquid chromatography (HPLC). Two forms of free monosaccharides (glucose and fructose), two sugar alcohol derivatives (inositol and glycerol), and seven forms of free oligosaccharides were identified. Of those, glucose content was the highest. LEDs did not disturb the sugar metabolism in multiplied shoots. Their monosaccharides were three times more abundant than oligosaccharides; the same results were found in plants grown under control light. R light depleted the performance of the photosynthetic apparatus and caused its permanent damage. The RB LED spectrum ensured the most efficient non-photochemical quenching of the photosystem II (PS II) excitation state and high shoot quality.

scholarly journals EFECTO DE LA FUENTE DE LUZ SOBRE LA MICROPROPAGACIÓN DE PLANTAS DEORÉGANO MEXICANO(Lippia graveolens)

e-CUCBA ◽  
2021 ◽  
Vol 15 (8) ◽  
pp. 44-52
Author(s):  
Sánchez-Velázquez Emmanuel ◽  
◽  
Rodríguez-Sahagún AracelI ◽  
Acevedo-Hernández Gustavo J ◽  
Rayn C Aarland ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Broad Spectrum ◽  
Energy Demand ◽  
The Other ◽  
Light Sources ◽  
Similar Response ◽  
Led Light ◽  
Fluorescent Lamps ◽  
Chlorophyll A And B

The use of different light sources in the micropropagation of plant species has been studied since the 1970s. At present, studies are being carried out not only to increase production, but to greatly reduce the energy demand of the lamps that are used for this purpose. In the present work, light sources with different wavelengths in fluorescent lamps and LED light were used to test the morphological and phytochemical response of Mexican origanumplants. A similar response was obtained regarding the production of axillary buds and leaves with the use of broad spectrum light and LED light. On the other hand, the main photosynthetic pigments (chlorophyll a and b and carotenoids) are also produced in a similar way when this plant is propagated under broad spectrum light and LED light.

scholarly journals The effect of led light sources with varied spectral composition on the in vitro rooting bility of garden strawberry (Fragaria × ananassa)

2019 ◽  
Vol 180 (1) ◽  
pp. 33-37 ◽  
Author(s):  
I. A. Byadovsky
Keyword(s):  
Red Light ◽  
Spectral Composition ◽  
Light Flux ◽  
Light Sources ◽  
Fragaria Ananassa ◽  
Fluorescent Lamp ◽  
Led Light ◽  
Rooting Ability ◽  
Strawberry Cultivars

Consideration is given to the possibility of using LED light sources with different ratios of red (650 - 660 nm), orange (600 - 610 nm) and blue (440 - 450 nm) light in the light flux in the rooting stage of garden strawberry (Fragaria×ananassa(Duch. ex Weston) Duch. ex Rozier) grownin vitro. The study covered a sizable diversity of strawberry cultivars (selected from thein vitrocollection): ‘Borovitskaya’, ‘Vityaz’, ‘Zenga Zengana’, ‘Khoney’, ‘Tsaritsa’, ‘Kubata’, ‘Dukat’, ‘Marmalada’, ‘Valenta’, ‘Rusich’, ‘Krasavitsa Zagorya’, ‘Neznakomka’ and ‘Roksana’, thus ensuring quite diversified results. It was established that the use of LED sources with an increased content of red light in the light flux increased the rooting percentage (1.15-1.25 times) and the number of roots per one microcutting (1.29-1.77 times) inin vitroculture, as compared with fluorescent lamp lighting. It was observed that with different spectral compositions of light the rooting ability of microcuttings was cultivar-specific.

scholarly journals Comparative Growth, Photosynthetic Pigments, and Osmolytes Analysis of Hemp (Cannabis sativa L.) Seedlings under an Aeroponics System with Different LED Light Sources

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 239
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Md. Soyel Rana ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Cannabis Sativa ◽  
Leaf Length ◽  
Future Research ◽  
Soluble Carbohydrate ◽  
Natural Light ◽  
Light Sources ◽  
Node Number ◽  
Led Light ◽  
Baseline Information

The performance of hemp seedlings was evaluated through morphological traits, photosynthetic pigments, and osmolytes under 11 light treatments (10 LED light compositions + natural light) in an aeroponics system. The seedlings were brought under treatment at 25 days of age, where the light intensity was 300 µmol m−2s−1 and duration was 20 days. A higher leaf number and node number were observed in L10 (R4:B2:W2:FR1:UV1) and L11 (R2:B2:G2:W2:FR1:UV1), and a higher leaf length and leaf width were recorded in the L2 (white), L3 (R8:B2), and L5 (R7:B2:FR1) treatments. Furthermore, a higher shoot length was recorded in L3 (R8:B2), L6 (R6:B2:G1:FR1), and L9 (R6:B2:FR1:UV1) while roots developed more in the L1 (natural light), L5 (R7:B2:FR1), and L9 (R6:B2:FR1:UV1) treatments. On the other hand, the L3 (R8:B2) treatment manifested higher chlorophyll a, chlorophyll b, and photosynthetic quantum yield (Fv/Fm). The hierarchical clustering and heatmap analysis revealed that higher leaf numbers and node numbers resulted in bushy plants with shorter shoots and longer roots. A negative correlation was also observed in photosynthetic traits (pigments and fluorescence) with osmolytes and root length. Importantly, the treatments L4 (R7:B2:G1), L6 (R6:B2:G1:FR1), L8 (R5:B2:G1:FR1:UV1), and L11 (R2:B2:G2:W2:FR1:UV1) manifested higher nodes with a higher osmolyte content, such as proline, ascorbic acid, total soluble carbohydrate, and sucrose, which may be a helpful indicator for higher branches and inflorescences, and ultimately higher cannabinoids accumulation in the plants. The approach and findings of this study could provide future research with the baseline information on optimizing the light composition to produce hemp plants with ideal phenotypes.

scholarly journals The influence of different wavelengths of LED light on the production of glucosinolates and phenolic compounds and the antioxidant potential in in vitro cultures of Nasturtium officinale (watercress)

2021 ◽  
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Barbara Prokopiuk ◽  
Kinga Dziurka ◽  
Bożena Pawłowska ◽  
Halina Ekiert ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Blue Light ◽  
Photosynthetic Pigments ◽  
Soluble Sugars ◽  
Antioxidant Potential ◽  
Fluorescent Light ◽  
Biomass Growth ◽  
Led Light ◽  
Nasturtium Officinale

AbstractCultures of Nasturtium officinale were cultivated in vitro under illumination with different wavelengths of light-emitting diode (LED) light (white LED light—WLED, blue light—B, red light—R, 70% red and 30% blue light—RB, 50% green, 35% red and 15% blue light—RBG, 50% yellow, 35% red and 15% blue light—RBY, 50% far red, 35% red and 15% blue light—RBfR, 50% UV, 35% red and 15% blue light—RBUV, darkness—D), and under white fluorescent light (WF)—control conditions. The study investigated the influence of the applied lighting conditions on biomass growth and the production of glucosinolates, phenolic compounds, as well as photosynthetic pigments, and soluble sugars. The study showed a significant beneficial effect of the RBG light on biomass growth (Gi = 11.81 after 20 days) and the production of glucosinolates. The total glucosinolate content under these conditions increased 5.8 and 1.4 times in comparison with the WF light and D condition, respectively, reaching 237.92 mg 100 g−1 DW. The production of phenolic compounds, sugars, and photosynthetic pigments was comparable to the production under the control conditions. The antioxidant potential of extracts from the cultivated biomass was assessed by the CUPRAC, DPPH, and FRAP assays. Extracts obtained from the biomass of cultures grown under the RBG light had an antioxidant potential similar to that of the control cultures. This is the first report providing evidence of the stimulating effect of light quality on the biomass yield and production of glucosinolates by N. officinale microshoot cultures in vitro.

scholarly journals Effect of led light on in vitro growth and developmentof Anoectochilus roxburghii

2018 ◽  
Vol 15 (1) ◽  
pp. 97-104
Author(s):  
Đỗ Thị Gấm ◽  
Chu Hoàng Hà Chu Hoàng Hà Chu Hoàng Hà ◽  
Phạm Bích Ngọc Phạm Bích Ngọc Phạm Bích Ngọc ◽  
Nguyễn Khắc Hưng ◽  
Phan Hồng Khôi ◽  
...  
Keyword(s):  
Growth And Development ◽  
Red Light ◽  
Light Condition ◽  
Luminous Intensity ◽  
Fluorescent Light ◽  
Light Sources ◽  
Plant Weight ◽  
Led Light ◽  
Anoectochilus Roxburghii

Recently, LED lighting technology has been developed fast and strongly. It has been widely used in many new fields. LEDs have been tested as an artificial light source during micropagation and tissue culture of various plants species as an alternative to traditional light sources to save energy and improve the efficiency of the culture process. In this study, effects of LED light at different wavelengths and luminous intensities on growth of Anoectochilus roxburghii's buds in vitro were investigated. Anoectochilus's buds were cultured under the different light conditions, such as LED in monochromatic red (R), LED in monochromatic green (B) , LED in the green and red light combining with warm white light (W) in different ratios (BRW 1, BRW2, BRW3 and BR). After 3 months of culture, the results showed LED B at strong luminous intensity (79 ± 3 μmol.m-2.s-1) inhibited the growth and development of Anoectochilus roxburghii. In contrast, BR LED with low luminous intensity (30 ± 1μmol.m-2.s-1) had made a positive effect on growth and development of Anoectochilus roxburghii. The plant height (5,88 cm), root length (1,33 cm), fresh plant weight (0,169 g/plant), leaf area (0,82 cm2), fresh leaf weight (18,33 mg/leaves) of plants in vitro were found higher than those of plants grown under T5 fluorescent lighting conditions (as control). Besides, chlorophyll a, chlorophyll b and total chlorophyll content of leaves (285,40 µg/g, 196,40 µg/g, 481,80 µg/g respectively) were higher than in the control and other versions of LED combine. The results also showed that the LED light with combined ratio of BR = 1: 4 at luminous intensity of 30 μmol.m-2.s-1 were suitable for the growth of Anoectochilus roxburghii. Therefore, BR LED (1:4) light condition should be replaced fluorescent light sources in vitro culture of Anoectochilus roxburghii in future.

Effects of gamma ray on some molecular characteristics of in vitro regenerated Gerbera jamesonii Bolus

1970 ◽  
Vol 39 (2) ◽  
pp. 207-214
Author(s):  
Nazma Akter ◽  
RH Sarker ◽  
MI Hoque
Keyword(s):  
Gamma Ray ◽  
Molecular Characteristics ◽  
Gerbera Jamesonii

DOI: 10.3329/bjb.v39i2.7490Bangladesh J. Bot. 39(2): 207-214, 2010 (December)

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