The impact of scald development on phenylpropanoid metabolism based on phenol content, enzyme activity, and gene expression analysis

Pseudomonas aeruginosa is one of the most common pathogenic bacteria that cause nosocomial infection. Unfortunately, the irrational use of antibiotics has created a surge in P. aeruginosa resistance nowadays. To overcome this situation, new antibacterial compounds are urgently needed. One of the potential sources to obtain such antibacterial compounds is roselle calyx. This research was carried out using two experimental approaches, survival assay and gene expression analysis, to examine the in vivo antibacterial effect of water fraction of roselle calyx (WFR) against Pseudomonas aeruginosa in Drosophila model of infection. Survival assay was used to demonstrate the impact of treatment on the lifespan of the infected host. The measurement of immune-related Dpt mRNA levels by reverse-transcriptase quantitative PCR (RT-qPCR) was used to assess whether immunostimulation is involved in the antibacterial protection of WFR against P. aeruginosa. The result demonstrated that WFR at concentrations of 0.8% and 2% were able to enhance P. aeruginosa-infected flies' survival. Furthermore, gene expression analysis showed the insignificant difference between WFR-treated flies and healthy control flies at all tested concentrations, implying the non-involvement of Imd-Dpt-mediated pathway immunity in the antipseudomonal protection of WFR. Taken together, our data suggested the in vivo antibacterial activity of WFR against P. aeruginosa in the fruit fly model of infection.

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Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

BMC Plant Biology ◽

10.1186/s12870-021-03275-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Mehdi Rahimi ◽

Mojtaba Kordrostami ◽

Fereshteh Mohamadhasani ◽

Sanam Safaei Chaeikar

Keyword(s):

Gene Expression ◽

Antioxidant Activity ◽

Antioxidant Enzymes ◽

Drought Stress ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Stress Conditions ◽

Total Phenol Content ◽

Phenol Content ◽

Oxygen Scavenging

Abstract Background Abiotic and biotic stresses induce oxidative processes in plant cells that this process starts with the production of ROSs which cause damage to the proteins. Therefore, plants have increased their antioxidant activity to defend against this oxidative stress to be able to handle stress better. In this research, 14 different tea accessions in a randomized complete block design with two replications were evaluated in two normal and drought stress conditions, and their antioxidant activity was measured by DPPH-free radicals’ assay and gene expression analysis. Results The results of gene expression analysis showed that the 100 and 399 accessions and Bazri cultivar had high values for most of the antioxidant enzymes, ascorbate peroxidase, superoxide dismutase, catalase, and peroxidase under drought stress conditions while the 278 and 276 accessions had the lowest amount of antioxidant enzymes in the same situation. Results showed that the IC50 of the BHT combination was 90.12 μg/ ml. Also, The IC50 of accessions ranged from 218 to 261 μg/ml and 201–264 μg/ml at normal and drought stress conditions, respectively. The 100 and 399 accessions showed the lowest IC50 under normal and drought stress conditions, while 278 and 276 accessions had the highest value for IC50. The antioxidant activity of tea accession extracts under normal conditions was ranged from 25 to 69% for accessions 278 and 100, respectively. While, the antioxidant activities of extracts under drought stress condition was 12 to 83% for accessions 276 and 100, respectively. So, according to the results, 100 and 399 accessions exhibited the least IC50 and more antioxidant activity under drought stress conditions and were identified as stress-tolerant accessions. However, 278 and 276 accessions did not show much antioxidant activity and were recognized as sensitive accessions under drought stress conditions. Conclusions These results demonstrate that total phenol content, antioxidant activity, and the oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions.

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The impact of quantitative optimization of hybridization conditions on gene expression analysis

BMC Bioinformatics ◽

10.1186/1471-2105-12-73 ◽

2011 ◽

Vol 12 (1) ◽

Cited By ~ 11

Author(s):

Peter Sykacek ◽

David P Kreil ◽

Lisa A Meadows ◽

Richard P Auburn ◽

Bettina Fischer ◽

...

Keyword(s):

Gene Expression ◽

Expression Analysis ◽

Gene Expression Analysis ◽

The Impact

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Gene Expression Analysis of Troglitazone Reveals Its Impact on Multiple Pathways in Cell Culture: A Case for In Vitro Platforms Combined with Gene Expression Analysis for Early (Idiosyncratic) Toxicity Screening

International Journal of Toxicology ◽

10.1080/10915810600605690 ◽

2006 ◽

Vol 25 (2) ◽

pp. 85-94 ◽

Cited By ~ 20

Author(s):

Gordon Vansant ◽

Patrick Pezzoli ◽

Robert Saiz ◽

Aaron Birch ◽

Chris Duffy ◽

...

Keyword(s):

Gene Expression ◽

Type 2 Diabetes ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Peroxisome Proliferator ◽

Toxicity Screening ◽

The Impact

Peroxisome proliferator-activated receptor gamma (PPAR γ) agonists of the thiazolidinedione family are used for the treatment of type 2 diabetes mellitus due to their ability to reduce glucose and lipid levels in patients with this disease. Three thiazolidinediones that were approved for treatment are Rezulin (troglitazone), Avandia (rosiglitazone), and Actos (pioglitazone). Troglitazone was withdrawn from the market due to idiosyncratic drug toxicity. Rosiglitazone and pioglitazone are still on the market for the treatment of type 2 diabetes. The authors present data from a gene expression screen that compares the impact these three compounds have in rats, in rat hepatocytes, and in the clone 9 rat liver cell line. The authors monitored the changes in expression in multiple genes, including those related to xenobiotic metabolism, proliferation, DNA damage, oxidative stress, apoptosis, and inflammation. Compared to the other two compounds, troglitazone had a significant impact on many of the pathways monitored in vitro although no major perturbation was detected in vivo. The changes detected predict not only general toxicity but potential mechanisms of toxicity. Based on gene expression analysis, the authors propose there is not just one but multiple ways troglitazone could be toxic, depending on a patient’s environment and genetic makeup, including immune response-related toxicity.

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The impact of gene expression analysis on evolving views of avian brain organization

The Journal of Comparative Neurology ◽

10.1002/cne.23403 ◽

2013 ◽

Vol 521 (16) ◽

pp. 3604-3613 ◽

Cited By ~ 26

Author(s):

Juan F. Montiel ◽

Zoltán Molnár

Keyword(s):

Gene Expression ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Avian Brain ◽

Brain Organization ◽

The Impact

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The impact of acute-stressor exposure on splenic innate immunity: A gene expression analysis

Brain Behavior and Immunity ◽

10.1016/j.bbi.2011.08.006 ◽

2012 ◽

Vol 26 (1) ◽

pp. 142-149 ◽

Cited By ~ 12

Author(s):

Thomas Maslanik ◽

Isaac Bernstein-Hanley ◽

Bryan Helwig ◽

Monika Fleshner

Keyword(s):

Gene Expression ◽

Innate Immunity ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Acute Stressor ◽

The Impact

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85. The impact of stressor exposure on innate immunity: A gene expression analysis

Brain Behavior and Immunity ◽

10.1016/j.bbi.2009.06.090 ◽

2009 ◽

Vol 23 ◽

pp. S48 ◽

Cited By ~ 1

Author(s):

T. Maslanik ◽

I. Bernstein-Hanley ◽

B. Helwig ◽

M. Fleshner

Keyword(s):

Gene Expression ◽

Innate Immunity ◽

Expression Analysis ◽

Gene Expression Analysis ◽

The Impact

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Activation of Caspase-3 by Terpenoids and Flavonoids in Different Types of Cancer Cells

Current Topics in Medicinal Chemistry ◽

10.2174/1568026620666200710101859 ◽

2020 ◽

Vol 20 (21) ◽

pp. 1876-1887

Author(s):

Nusrat Masood ◽

Vijaya Dubey ◽

Suaib Luqman

Keyword(s):

Gene Expression ◽

Natural Products ◽

Enzyme Activity ◽

Expression Analysis ◽

Anticancer Agents ◽

Gene Expression Analysis ◽

Caspase 3 ◽

Mrna Levels ◽

Promising Target ◽

Mcf 7

Background: Caspase-3 is accountable for the execution of apoptosis. Recently, it has gained attention as a promising target for the discovery of natural products as anticancer agents. Methods: We examined the efficacy of two different sets of natural products (terpenoids and flavonoids) towards caspase-3 activity adopting in silico, cell-free and cell-based activity and real-time gene expression analysis. Results: It was observed that terpenes activate caspase-3 activity in both the cell-free and cell-based systems, which was supported by the gene expression analysis, binding energy and activation constant. Flavonoids’ action, however, was limited to the cell-based system and transcriptional regulation suggesting their indirect association, which enhanced the enzyme activity and up-regulated the expression of mRNA levels in the cells. Among the tested natural products, (+) carvone was observed to be the best activator of caspase-3 in K562 (34.4 μM), WRL-68 (22.3 μM), HeLa (18.7 μM), MCF-7 (39.4 μM) and MDA-MB-231 cell lines (45.1 μM). Conclusion: Overall, terpenoids have a persistent activation of caspase-3 in all the investigated systems, while flavonoids circuitously affect the enzyme activity.

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