3(2) Designing and optimization of aceclofenac transdermal films using response surface methodology: investigating the effect of hydrophilic and hydrophobic matrix on ex vivo and in vivo permeation characteristics

2016 ◽  
Vol 47 (6) ◽  
pp. 541-558 ◽  
Author(s):  
Pragya Yadav ◽  
Vaibhav Rastogi ◽  
Mayur Porwal ◽  
Prashant Upadhyay ◽  
Anurag Verma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ex Vivo ◽  
Hydrophobic Matrix

scholarly journals Optimization of Synthesis of Seleno-Sargassum fusiforme(Harv.) Setch. Polysaccharide by Response Surface Methodology, Its Characterization, and Antioxidant Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Bin Ji ◽  
Fang Dong ◽  
Miao Yu ◽  
Long Qin ◽  
Dan Liu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Reaction Time ◽  
Response Surface ◽  
Reaction Temperature ◽  
Sargassum Fusiforme ◽  
Synthesis Conditions ◽  
Optimum Reaction ◽  
Series Of Experiments

The response surface methodology was employed to optimize the synthesis conditions of seleno-Sargassum fusiforme(Harv.) Setch. polysaccharide. Three independent variables (reaction time, reaction temperature, and ratio of Na2SeO3to SFPSI) were tested. Furthermore, the characterization and antioxidant activity of Se-SFPSIin vivowere investigated. The result showed that the actual experimental Se content of Se-SFPSI was 3.352 mg/g at the optimum reaction conditions of reaction time 8 h, reaction temperature 71°C, and ratio of Na2SeO3to SFPSIB 1.0 g/g. A series of experiments showed that the characterization of Se-SFPSIB was significantly different from that of SFPSIB. Additionally, antioxidant activity assay indicated that the Se-SFPSIB could increase catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity of mice bearing tumor S180in blood, heart, and liver while decreasing malondialdehyde (MDA) levels. It can be concluded that selenylation is a feasible approach to obtain seleno-polysaccharide which was utilized as highly biological medicine or functional food.

scholarly journals Modulation of Drug Release from Natural Polymer Matrices by Response Surface Methodology: in vitro and in vivo Evaluation

2020 ◽  
Vol Volume 14 ◽  
pp. 5325-5336
Author(s):  
Afrasim Moin ◽  
Hosahalli V Gangadharappa ◽  
Mohd Adnan ◽  
Syed M Rizvi ◽  
Syed A Ashraf ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Drug Release ◽  
Response Surface ◽  
Polymer Matrices ◽  
Natural Polymer ◽  
In Vivo Evaluation

scholarly journals Optimization of Mangifera indica L. Kernel Extract-Loaded Nanoemulsions via Response Surface Methodology, Characterization, Stability, and Skin Permeation for Anti-Acne Cosmeceutical Application

Pharmaceutics ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 454 ◽  
Author(s):  
Worrapan Poomanee ◽  
Watcharee Khunkitti ◽  
Wantida Chaiyana ◽  
Pimporn Leelapornpisid
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ex Vivo ◽  
Mangifera Indica ◽  
Skin Permeation ◽  
Skin Barrier ◽  
The Stability ◽  
Spherical Droplets ◽  
Central Composite ◽  
Excellent Stability

This study aimed to optimize nanoemulsions loading with Mangifera indica L. kernel extract using response surface methodology for enhancing the stability and skin permeation of the extract. Central composite design was employed for optimization and evaluation of three influencing factors including hydrophile-lipophile balance (HLB), % co-surfactant (PEG-7 glyceryl cocoate), and surfactant-to-oil ratio (SOR) on physical properties of the nanoemulsions. The desired nanoemulsions were then incorporated with the extract and characterized. Physicochemical properties of the extract-loaded nanoemulsions and their antibacterial effects against Propionibacterium acnes were also evaluated after storage at various conditions and compared to those of the initial. Ex vivo skin permeation was also investigated. The factors significantly (p < 0.05) influenced on droplet size, polydispersity index (PDI), and zeta potential, especially HLB of the surfactant and its combined effects with co-surfactant and SOR. The extract-loaded nanoemulsions revealed a very small spherical droplets (size of 26.14 ± 0.22 nm) with narrow size distribution (PDI of 0.16 ± 0.02). The formulation also presented an excellent stability profile and successfully enhanced antibacterial stability of the extract comparing with the extract solution. Ex vivo skin permeation study illustrated that the extract in nanoemulsions could be delivered through a primary skin barrier to reach viable epidermis dermis layers. In conclusion, the affinity of surfactant and hydrophilicity of the system play a crucial role in nanoemulsions’ characteristics. Such results might provide promising anti-acne nanoemulsions with the notable capacities of extract stabilization and permeation enhancing which will be further clinically evaluated.

scholarly journals Statistical Optimization of Copper Oxide Nanoparticles Using Response Surface Methodology and Box–Behnken Design Towards In Vitro and In Vivo Toxicity Assessment

2020 ◽  
Vol 11 (3) ◽  
pp. 10027-10039
Keyword(s):  
Response Surface Methodology ◽  
Copper Oxide ◽  
Response Surface ◽  
Copper Oxide Nanoparticles ◽  
Toxicity Assessment ◽  
P Value ◽  
Oxide Nanoparticles ◽  
Box Behnken Design

The current study focusses on the optimization of Copper oxide nanoparticles (CuO NPs) biosynthesis with Alternanthera sessilis (L.) extract using response surface methodology (RSM). The effect of time, pH, and extract to metal concentration ratio on the yield of synthesized nanoparticles (NPs) were estimated using Box–Behnken design. The influence of each of the parameters, as mentioned earlier, was determined by synthesizing nanoparticles under different conditions. A total of 29 experimental runs were carried out to estimate the crucial parameters. Extract to the metal ratio was found to be the vital parameter for yield optimization based on the p-values (p-value < 0.05). The physicochemical property of NPs, like size, was estimated to be in the range of 10-20 nm. In zebrafish, 48 hpf and 72 hpf were measured at 90 µM to reduce dysfunction and mortality during organ development. These results can have a valuable impact on eco-toxicological effects.

scholarly journals Optimization on Preparation Condition of Propolis Flavonoids Liposome by Response Surface Methodology and Research of Its Immunoenhancement Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ju Yuan ◽  
Yu Lu ◽  
Saifuding Abula ◽  
Yuanliang Hu ◽  
Jiaguo Liu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Theoretical Basis ◽  
Encapsulation Efficiency ◽  
Preparation Condition ◽  
Ethanol Injection ◽  
Preparation Conditions ◽  
Soybean Phospholipid

The aim of this study is to prepare propolis flavonoids liposome (PFL) and optimize the preparation condition and to investigate further whether liposome could promote the immunoenhancement activity of propolis flavonoids (PF). PFL was prepared with ethanol injection method, and the preparation conditions of PFL were optimized with response surface methodology (RSM). Moreover, the immunoenhancement activity of PFL and PFin vitrowas determined. The result showed that the optimal preparation conditions for PFL by response surface methodology were as follows: ratio of lipid to drug (w/w) 9.6 : 1, ratio of soybean phospholipid to cholesterol (w/w) 8.5 : 1, and speed of injection 0.8 mL·min−1. Under these conditions, the experimental encapsulation efficiency of PFL was 91.67 ± 0.21%, which was close to the predicted value. Therefore, the optimized preparation condition is very reliable. Moreover, the results indicated that PFL could not only significantly promote lymphocytes proliferation singly or synergistically with PHA, but also increase expression level of IL-2 and IFN-γmRNA. These indicated that liposome could significantly improve the immunoenhancement activity of PF. PFL demonstrates the significant immunoenhancement activity, which provides the theoretical basis for the further experimentin vivo.

Response Surface Methodology: An Extensive Potential to Optimize in vivo Photodynamic Therapy Conditions

2009 ◽  
Vol 75 (1) ◽  
pp. 244-252 ◽  
Author(s):  
Loraine Tirand ◽  
Thierry Bastogne ◽  
Denise Bechet ◽  
Michel Linder ◽  
Noémie Thomas ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Photodynamic Therapy ◽  
Response Surface

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

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