Persistence of Tannerella forsythia and Fusobacterium nucleatum in Dental Plaque: a Strategic Alliance

2020 ◽  
Vol 7 (1) ◽  
pp. 22-28
Author(s):  
Ashu Sharma
Keyword(s):  
Dental Plaque ◽  
Strategic Alliance ◽  
Fusobacterium Nucleatum ◽  
Tannerella Forsythia

scholarly journals β-Glucanase Activity of the Oral Bacterium Tannerella forsythia Contributes to the Growth of a Partner Species, Fusobacterium nucleatum, in Cobiofilms

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
Kiyonobu Honma ◽  
Angela Ruscitto ◽  
Ashu Sharma
Keyword(s):  
Periodontal Disease ◽  
Sigma Factor ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Disease Pathogenesis ◽  
Glucanase Activity ◽  
Tannerella Forsythia ◽  
Content Type ◽  
Plaque Development ◽  
Oral Bacterium

ABSTRACTTannerella forsythiaandFusobacterium nucleatumare dental plaque bacteria implicated in the development of periodontitis. These two species have been shown to form synergistic biofilms and have been found to be closely associated in dental plaque biofilms. A number of genetic loci for TonB-dependent membrane receptors (TDR) for glycan acquisition, with many existing in association with genes coding for enzymes involved in the breakdown of complex glycans, have been identified inT. forsythia. In this study, we focused on a locus, BFO_0186-BFO_0188, that codes for a predicted TDR-SusD transporter along with a putative β-glucan hydrolyzing enzyme (BFO_0186). This operon is located immediately downstream of a 2-gene operon that codes for a putative stress-responsive extracytoplasmic function (ECF) sigma factor and an anti-sigma factor. Here, we show that BFO_0186 expresses a β-glucanase that cleaves glucans with β-1,6 and β-1,3 linkages. Furthermore, the BFO_0186-BFO_0188 locus is upregulated, with an induction of β-glucanase activity, in cobiofilms ofT. forsythiaandF. nucleatum. The β-glucanase activity in mixed biofilms in turn leads to an enhanced hydrolysis of β-glucans and release of glucose monomers and oligomers as nutrients forF. nucleatum. In summary, our study highlights the role ofT. forsythiaβ-glucanase expressed by the asaccharolytic oral bacteriumT. forsythiain the development ofT. forsythia-F. nucleatummixed species biofilms, and suggest that dietary β-glucans might contribute in plaque development and periodontal disease pathogenesis.IMPORTANCEThe development of dental plaque biofilm is a complex process in which metabolic, chemical and physical interactions between bacteria take a central role. Previous studies have shown that the dental pathogensT. forsythiaandF. nucleatumform synergistic biofilms and are closely associated in human dental plaque. In this study, we show that β-glucanase from the periodontal pathogenT. forsythiaplays a role in the formation ofT. forsythia-F. nucleatumcobiofilms by hydrolyzing β-glucans to glucose as a nutrient. We also unveiled that the expression ofT. forsythiaβ-glucanase is induced in response toF. nucleatumsensing. This study highlights the involvement of β-glucanase activity in the development ofT. forsythia-F. nucleatumbiofilms and suggests that intake of dietary β-glucans might be a contributing risk factor in plaque development and periodontal disease pathogenesis.

scholarly journals Bacteriocin-like activity of oral Fusobacterium nucleatum isolated from human and non-human primates

1999 ◽  
Vol 30 (4) ◽  
pp. 324-346 ◽  
Author(s):  
Elerson Gaetti-Jardim Júnior ◽  
Mario Julio Avila-Campos
Keyword(s):  
Oral Cavity ◽  
Microbial Ecology ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Antagonistic Activity ◽  
Healthy Individuals ◽  
Bacterial Microbiota ◽  
Reference Strains

Fusobacterium nucleatum is indigenous of the human oral cavity and has been involved in different infectious processes. The production of bacteriocin-like substances may be important in regulation of bacterial microbiota in oral cavity. The ability to produce bacteriocin-like substances by 80 oral F. nucleatum isolates obtained from periodontal patients, healthy individuals and Cebus apella monkeys, was examinated. 17.5% of all tested isolates showed auto-antagonism and 78.8% iso- or hetero-antagonism. No isolate from monkey was capable to produce auto-inhibition. In this study, the antagonistic substances production was variable in all tested isolates. Most of the F. nucleatum showed antagonistic activity against tested reference strains. These data suggest a possible participation of these substances on the oral microbial ecology in humans and animals. However, the role of bacteriocins in regulating dental plaque microbiota in vivo is discussed.

scholarly journals Mutualistic Biofilm Communities Develop with Porphyromonas gingivalis and Initial, Early, and Late Colonizers of Enamel

2009 ◽  
Vol 191 (22) ◽  
pp. 6804-6811 ◽  
Author(s):  
Saravanan Periasamy ◽  
Paul E. Kolenbrander
Keyword(s):  
Porphyromonas Gingivalis ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Single Species ◽  
Biofilm Growth ◽  
Flow Cells ◽  
Tooth Cleaning ◽  
Plaque Development ◽  
Species Specific

ABSTRACT Porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. We report here that P. gingivalis ATCC 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. Integration of P. gingivalis into multispecies communities was investigated by using two in vitro biofilm models. In flow cells, bacterial growth was quantified using fluorescently conjugated antibodies against each species, and static biofilm growth on saliva-submerged polystyrene pegs was analyzed by quantitative real-time PCR using species-specific primers. P. gingivalis could not grow as a single species or together with initial colonizer Streptococcus oralis but showed mutualistic growth when paired with two other initial colonizers, Streptococcus gordonii and Actinomyces oris, as well as with Veillonella sp. (early colonizer), Fusobacterium nucleatum (middle colonizer), and Aggregatibacter actinomycetemcomitans (late colonizer). In three-species flow cells, P. gingivalis grew with Veillonella sp. and A. actinomycetemcomitans but not with S. oralis and A. actinomycetemcomitans. Also, it grew with Veillonella sp. and F. nucleatum but not with S. oralis and F. nucleatum, indicating that P. gingivalis and S. oralis are not compatible. However, P. gingivalis grew in combination with S. gordonii and S. oralis, demonstrating its ability to overcome the incompatibility when cultured with a second initially colonizing species. Collectively, these data help explain the observed presence of P. gingivalis at all stages of dental plaque development.

Desulfuration of Cysteine and Methionine by Fusobacterium nucleatum

1986 ◽  
Vol 65 (6) ◽  
pp. 913-917 ◽  
Author(s):  
R. Pianotti ◽  
S. Lachette ◽  
S. Dills
Keyword(s):  
Pyruvic Acid ◽  
Substrate Concentration ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Intact Cells ◽  
Gram Negative ◽  
Hydrolysis Products ◽  
Volatile Products ◽  
Excess Substrate ◽  
Sulfur Containing

Fusobacterium nucleatum is a Gram-negative anaerobic rod-shaped bacterium frequently isolated from human dental plaque. It is capable of the desulfuration of cysteine and methionine, resulting in the formation of sulfide and thiol volatiles, respectively. Intact cells, as well as cell-free extracts produced by French pressure cell lysis of F. nucleatum, hydrolyzed radiolabeled cysteine to produce sulfide, pyruvic acid, and ammonia. The hydrolysis products of radiolabeled methionine were a volatile thiol, ketobutyrate, and ammonia. Both activities were associated with the cytoplasmic component, not the membrane. The desulfuration mechanisms are heat-labile, inhibited by the presence of excess substrate, and rates are dependent upon substrate concentration. These dissimilar pathways by F. nucleatum can account in part for the presence of sulfur-containing volatile products that occur in the mouth.

Synergy between Tannerella forsythia and Fusobacterium nucleatum in biofilm formation

2005 ◽  
Vol 20 (1) ◽  
pp. 39-42 ◽  
Author(s):  
A. Sharma ◽  
S. Inagaki ◽  
W. Sigurdson ◽  
H. K. Kuramitsu
Keyword(s):  
Biofilm Formation ◽  
Fusobacterium Nucleatum ◽  
Tannerella Forsythia

scholarly journals Fusobacterium nucleatum ATCC 10953 Requires Actinomyces naeslundii ATCC 43146 for Growth on Saliva in a Three-Species Community That Includes Streptococcus oralis 34

2009 ◽  
Vol 75 (10) ◽  
pp. 3250-3257 ◽  
Author(s):  
Saravanan Periasamy ◽  
Natalia I. Chalmers ◽  
Laurence Du-Thumm ◽  
Paul E. Kolenbrander
Keyword(s):  
Dental Plaque ◽  
Community Dynamics ◽  
Developmental Process ◽  
Fusobacterium Nucleatum ◽  
Fold Increase ◽  
Flow Cell ◽  
Pcr Analysis ◽  
Actinomyces Naeslundii ◽  
Streptococcus Oralis

ABSTRACT Formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. Fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, have established communities. The unusual ability of these bacteria to coaggregate with commensals, as well as pathogenic late colonizers, has been proposed to facilitate colonization by the latter organisms. We investigated the integration of Fusobacterium nucleatum into multispecies communities by employing two in vitro models with saliva as the sole nutritional source. In flow cell biofilms, numbers of cells were quantified using fluorescently conjugated antibodies against each species, and static biofilms were analyzed by quantitative real-time PCR (q-PCR) using species-specific primers. Unable to grow as single-species biofilms, F. nucleatum grew in two-species biofilms with Actinomyces naeslundii but not with Streptococcus oralis. However, enhanced growth of fusobacteria was observed in three-species biofilms, indicating that there was multispecies cooperation. Importantly, these community dynamics yielded an 18-fold increase in the F. nucleatum biomass between 4 h and 18 h in the flow cell inoculated with three species. q-PCR analysis of static biofilms revealed that maximum growth of the three species occurred at 24 h to 36 h. Lower numbers of cells were observed at 48 h, suggesting that saliva could not support higher cell densities as the sole nutrient. Integration of F. nucleatum into multispecies commensal communities was evident from the interdigitation of fusobacteria in coaggregates with A. naeslundii and S. oralis and from the improved growth of fusobacteria, which was dependent on the presence of A. naeslundii.

scholarly journals DETECTION OF BACTERIAL SPECIES ASSOCIATED WITH PERIODONTAL DISEASE IN A GROUP OF PATIENTS

2021 ◽  
Vol 67 (2) ◽  
pp. 128-134
Author(s):  
Cristina Vodiță ◽  
◽  
Doina Lucia Ghergic ◽  
Emanuel Alin Vodiță ◽  
Raluca Monica Comăneanu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Treatment Session ◽  
Prevotella Intermedia ◽  
Bacterial Strains ◽  
Tannerella Forsythia ◽  
Campylobacter Rectus ◽  
Number Of Patients

Objectives. The study aimed to detect the presence/quantification of the 12 bacterial strains more frequently involved in the occurrence and aggravation of periodontal disease in a group of patients. Material and method. The study included 55 patients of both sexes, from the portfolio of the „Dr. Vodiță“ Dental Clinic, with chronic marginal periodontitis, who had not been under regular dental control and hygiene for at least 2 years. During the first treatment session, fluid was collected from each patient from the periodontal bags using sterile paper cones from the collection putty. Subsequently, fluid-soaked paper cones from periodontal bags were sent to the Genetic Lab in Bucharest for DNA extraction and quantification of 12 bacterial species, more common in the etiology of periodontal disease. For the correctness of the results obtained, it is necessary that patients have not taken antibiotics in the last 3 weeks. The data were analyzed and statistically processed with the Microsoft Excel 2016 program. Results. The least common bacteria found were from the species: Capnocytophaga ochracea, Aggregatibacter actinomycetemcomitans, Eikanella corrodens and Campylobacter rectus, closely followed by Capnocytophaga gingivalis, Prevotella intermedia and Capnocytophaga sputigena. The rest of the bacterial species were detected much more frequently. Discussions. In the analyzed group there was no monoinfection with Aggregatibacter actinomycetemcomitans. Porphyromonas gingivalis had an important presence in the studied group, registering increased levels in 89% of cases. In our group, Treponema denticola was present in increased amounts in 76.36% of cases. Tannerella forsythia was present in 92.72% of cases in elevated concentrations. Eikanella corrodens and Campylobacter rectus were present in 18.18% of the cases studied in high quantities. Prevotella intermedia was present in significant amounts in 34.54% of cases. Fusobacterium nucleatum was present in high concentrations in 98.18% of the cases studied. Prevotella nigrescens was present in increased amounts in 56.36% of cases. Capnocytophaga ochracea was present in 9.09% of cases in high amounts, Capnocytophaga sputigena in 38.18% of cases, and Capnocytophaga gingivalis was detected in 24.45% of cases. Conclusions. From the class of bacteria with high pathogenicity, we most frequently encountered in the studied group Tannerella forsythia. From the class of bacteria with moderate pathogenicity, we encountered the most common Fusobacterium nucleatum. From the class of bacteria with low pathogenicity, we encountered the most common Capnocytophaga sputigena. In order to validate the results obtained, it is necessary to extend the study to a larger number of patients.

scholarly journals The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation

2013 ◽  
Vol 81 (4) ◽  
pp. 1198-1206 ◽  
Author(s):  
Naohiro Shimotahira ◽  
Yuichi Oogai ◽  
Miki Kawada-Matsuo ◽  
Sakuo Yamada ◽  
Kenji Fukutsuji ◽  
...  
Keyword(s):  
Surface Layer ◽  
Calf Serum ◽  
Fusobacterium Nucleatum ◽  
Oral Bacteria ◽  
Multiple Roles ◽  
Serum Resistance ◽  
Survival Ratio ◽  
Wild Type ◽  
Tannerella Forsythia ◽  
Content Type

ABSTRACTTannerella forsythiais an anaerobic, Gram-negative bacterium involved in the so-called “red complex,” which is associated with severe and chronic periodontitis. The surface layer (S-layer) ofT. forsythiais composed of cell surface glycoproteins, such as TfsA and TfsB, and is known to play a role in adhesion/invasion and suppression of proinflammatory cytokine expression. Here we investigated the association of this S-layer with serum resistance and coaggregation with other oral bacteria. The growth of the S-layer-deficient mutant in a bacterial medium containing more than 20% non-heat-inactivated calf serum (CS) or more than 40% non-heat-inactivated human serum was significantly suppressed relative to that of the wild type (WT). Next, we used confocal microscopy to perform quantitative analysis on the effect of serum. The survival ratio of the mutant exposed to 100% non-heat-inactivated CS (76% survival) was significantly lower than that of the WT (97% survival). Furthermore, significant C3b deposition was observed in the mutant but not in the WT. In a coaggregation assay, the mutant showed reduced coaggregation withStreptococcus sanguinis,Streptococcus salivarius, andPorphyromonas gingivalisbut strong coaggregation withFusobacterium nucleatum. These results indicated that the S-layer ofT. forsythiaplays multiple roles in virulence and may be associated with periodontitis.

scholarly journals Effect of sonic treatment on pure cultures and aggregates of bacteria

1976 ◽  
Vol 3 (5) ◽  
pp. 474-479
Author(s):  
S A Robrish ◽  
S B Grove ◽  
R S Bernstein ◽  
P T Marucha ◽  
S S Socransky ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Ultrasonic Energy ◽  
Sonic Treatment ◽  
Pure Cultures

Pure cultures of a variety of bacteria were treated with ultrasonic energy using a sonic probe. Fractions of organisms killed at different sonic energies were calculated, and Streptococcus mutans was 600 times more resistant than Fusobacterium nucleatum, the most sensitive organism tested. The effects of sonic treatment on aggregates of bacteria were examined, and the results were interpreted as a model of the events that probably occur during the sonic dispersion of dental plaque.

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