Exploring potential of hydro-alcoholic extract of stem of marjoram as natural preservative against food spoilage bacteria Bacillus cereus and Bacillus megaterium in homemade mango jam

Isolation, Characterization, and Application of Bacteriophages Against Several Food Spoilages Bacteria

10.21203/rs.3.rs-42622/v1 ◽

2020 ◽

Author(s):

Felicia Roseline Sujonoputri ◽

Diana Elizabeth Waturangi ◽

Yogiara Yogiara

Keyword(s):

Bacillus Subtilis ◽

Bacillus Cereus ◽

Soil Sample ◽

Shewanella Putrefaciens ◽

Food Preservation ◽

Food Spoilage ◽

Spoilage Bacteria ◽

Retail Food

Abstract Objective: This study was conducted to isolate bacteriophages from soil sample, retail food, and wastewater from fish and then the bacteriophages will be characterized for their activity against several food spoilage bacteria, such as Bacillus cereus, Bacillus subtilis, and Shewanella putrefaciens and will be further investigated for application as food preservation.Results: Total of four bacteriophages were isolated with B. cereus, B. subtilis, and S. putrefaciens as host bacteria. Bacteriophage titers observed around 109 PFU mL-1. Bacteriophages that isolated with B. cereus and B.subtilis as host bacteria tend to have high EOP with the same species bacteria. All the Bacillus phages (S1-BC, S2-BC, and S1-BS) can reduce the Bacillus species bacteria concentration for more than 90%. Refers to their activity, the isolated bacteriophages in this study have a great potential as biocontrol against several food spoilage bacteria.

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ISOLATION, CHARACTERIZATION, AND APPLICATION OF BACTERIOPHAGES AGAINST SEVERAL FOOD SPOILAGE BACTERIA: Bacillus subtilis, Bacillus cereus, AND Shewanella Putrefaciens

Bacterial Empire ◽

10.36547/be.263 ◽

2021 ◽

Vol 4 (2) ◽

pp. e263

Author(s):

Felicia Roseline ◽

Diana Elizabeth Waturangi ◽

Yogiara Y

Keyword(s):

Bacillus Subtilis ◽

Bacillus Cereus ◽

Shewanella Putrefaciens ◽

Black Soil ◽

Food Preservation ◽

Bacillus Species ◽

Wastewater Sample ◽

Food Spoilage ◽

Sample Collection ◽

Spoilage Bacteria

Introduction: Bacteriophages can be the natural alternative method to prevent food spoilage caused by bacteria. This research was conducted to isolate bacteriophages from a soil sample, retail food, and wastewater from fish and then the bacteriophages will be characterized for their activity against several food spoilage bacteria, such as Bacillus cereus, Bacillus subtilis, and Shewanella putrefaciens and will be further investigated for application as food preservation. Experimental: B. cereus (Atma Jaya culture collection), B. subtilis ATCC 6633, and S. putrefaciens ATCC 8071 were used for bacteriophage isolation. Food samples (rice, pasta, tofu, tempeh), soil samples (black soil and laterite), and wastewater sample of freshwater fish and seawater fish were used in this research. The overall process consists of eight steps: inoculum preparation, sample collection, bacteriophage isolation, purification and enrichment, titer determination, host range determination, the efficiency of plating (EOP), and bacteriophage application. Results: A total of four bacteriophages were isolated with B. cereus, B. subtilis, and S. putrefaciens as host bacteria. Bacteriophage titers observed around 109 PFU mL-1. Bacteriophages that isolated with B. cereus and B. subtilis as host bacteria tend to have high EOP with the same species bacteria. All the Bacillus phages (S1-BC, S2-BC, and S1-BS) can reduce the Bacillus species bacteria concentration for more than 90%. Conclusion: Refers to their activity, the isolated bacteriophages in this study might have a great prospect to be used as food biocontrol and also can be further tested to make a phage cocktail.

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Evaluation of Antibacterial Activity of Lactobacillus Spp. on Selected Food Spoilage Bacteria

Recent Patents on Food Nutrition & Agriculturee ◽

10.2174/2212798407666150309125110 ◽

2015 ◽

Vol 7 (1) ◽

pp. 9-13

Author(s):

Anurag Sharma ◽

Piyush Gupta ◽

Susinjan Bhattacharya

Keyword(s):

Antibacterial Activity ◽

Food Spoilage ◽

Spoilage Bacteria ◽

Lactobacillus Spp

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Identification of novel horA-harbouring bacteria capable of spoiling beer

Canadian Journal of Microbiology ◽

10.1139/w08-007 ◽

2008 ◽

Vol 54 (4) ◽

pp. 321-325 ◽

Cited By ~ 22

Author(s):

Monique Haakensen ◽

Barry Ziola

Keyword(s):

Drug Resistance ◽

Bacillus Cereus ◽

Bacillus Licheniformis ◽

Staphylococcus Epidermidis ◽

Multi Drug Resistance ◽

Bacterial Isolates ◽

First Finding ◽

Spoilage Bacteria ◽

Beer Spoilage ◽

Respective Species

An ATP-binding cassette (ABC) multi-drug resistance (MDR) gene was found in 4 Gram-positive bacterial isolates of environmental origin and found capable of spoiling beer. The bacteria isolated were Bacillus cereus , Bacillus licheniformis , Paenibacillus humicus , and Staphylococcus epidermidis ; all of which were previously unappreciated as beer-spoilage bacteria. The MDR gene found in these bacteria has less than 37% similarity to known ABC MDR proteins described for Bacillus and Staphylococcus , and this is the first finding of an ABC MDR gene in the genus Paenibacillus . The sequenced region of the gene was translated and compared phylogenetically with the closest GenBank matches of the respective species and the closest GenBank matches overall. The ABC MDR proteins from these isolates were found to cluster among known sequences of HorA, sharing 99.5% identity within the sequenced region. In the beer-spoilage-associated genera Lactobacillus and Pediococcus , the presence of the MDR gene horA correlates with the ability to grow in beer. As the unique horA-harbouring isolates described here are capable of growing in beer, it is likely that the presence of the horA gene likewise confers hop resistance to these organisms.

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Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae)

Jurnal Penelitian Pertanian Terapan ◽

10.25181/jppt.v17i2.294 ◽

2017 ◽

Vol 17 (2) ◽

Author(s):

I Made Indra Agastya ◽

Aminudin Afandhi ◽

Luqman Qurata Aini

Keyword(s):

Bacillus Cereus ◽

Bacillus Megaterium ◽

Spodoptera Litura ◽

Laboratory Studies ◽

Pests And Diseases ◽

Control Effectiveness ◽

Randomized Design ◽

Completely Randomized Design ◽

Lepidoptera Noctuidae

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura

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Chapter 9 Culture media for non-sporulating Gram positive, catalase positive food spoilage bacteria

Handbook of Culture Media for Food Microbiology - Progress in Industrial Microbiology ◽

10.1016/s0079-6352(03)80012-7 ◽

2003 ◽

pp. 141-145 ◽

Cited By ~ 1

Author(s):

GA Gardner

Keyword(s):

Culture Media ◽

Food Spoilage ◽

Gram Positive ◽

Spoilage Bacteria

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Antimicrobial Activity of neo-Clerodane Diterpenoids isolated from Lamiaceae Species against Pathogenic and Food Spoilage Microorganisms

Natural Product Communications ◽

10.1177/1934578x1501001101 ◽

2015 ◽

Vol 10 (11) ◽

pp. 1934578X1501001 ◽

Cited By ~ 1

Author(s):

Petko Bozov ◽

Tania Girova ◽

Natalia Prisadova ◽

Yana Hristova ◽

Velizar Gochev

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Food Spoilage ◽

Aerial Parts ◽

Spoilage Bacteria ◽

Microbial Strains ◽

Clerodane Diterpenoids ◽

Acetone Extracts ◽

And Staphylococcus Aureus ◽

Spoilage Microorganisms

Antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of Scutellaria and Salvia species (Lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria Aeromonas hydrophila, Bacillus cereus, Escherichia coli, Listeria monocytogenes, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella abony and Staphylococcus aureus as well as against two yeast strains belonging to species Candida albicans. Seven of the evaluated compounds scutalpin A, scutalpin E, scutalpin F, salviarin, splenolide A, splenolide B and splendidin demonstrated antimicrobial activity against used test microbial strains, the rest of the compounds were inactive within the studied concentration range. Among all of the tested compounds the highest antimicrobial activity was detected for scutalpin A against Staphylococcus aureus (MIC 25 μg/mL).

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