Combined antibacterial effect of Origanum compactum and Mentha piperita (Lamiaceae) essential oils against ATCC Escherichia coli and Staphylococcus aureus

Author(s):  
Soukaina El amrani ◽  
Lairini Sanae ◽  
Yassine Ez zoubi ◽  
Gulsun Akdemir Evrendilek ◽  
Fadil Mouhcine ◽  
...  
2014 ◽  
Vol 1052 ◽  
pp. 327-331 ◽  
Author(s):  
Jian Rong Wang ◽  
Ya Li Liu ◽  
Bin Liu ◽  
Yan Ping Wu

A novel method was used to make palygorskite supported nano-silver powder at room temperature. XRD result showed the presence of silver in the final product. TEM investigation revealed that nano-sliver particles of 6-10 nm in diameter were successfully attached on surface of palygorskite fiber. FTIR was also used to clarify the formation mechanism of our method. The antibacterial effect of palygorskite supported nano-silver powder was investigated. Palygorskite supported nano-silver powder had good antibacterial activity on standard Escherichia coli and Staphylococcus aureus.


2018 ◽  
Vol 3 (3) ◽  
pp. 116-120
Author(s):  
Nur Syafiqah Nadiah Mohammad Rafi ◽  
Irmanida Batubara ◽  
Anthony Nyangson Steven

This research aims to determine the antibacterial activity of Curcuma xanthorrhiza leaves and rhizomes essential oil with different distillation time of 2 hours, 4 hours and 6 hours against Escherichia coli and Staphylococcus aureus. The essential oils produced then separated by thin layer chromatography (TLC) with n-hexane: toluene: dichloromethane 1:8:1 as the mobile phase. The result shows that on the first two hours distillation gave the highest yield compared to the next two hours or four hours. Based on TLC chromatograms, the highest content of compounds found at 4-6 hours distillation time. TLC bioautography contact was used for antibacterial activity tested on chromatogram and the clear zone appears on it indicates the spots were active as antibacterial activity against both bacteria with Rf values of 0.35 and 0.49 for rhizomes, rhizome essential oils 4-6 hours distillation time as the most significance active.


2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Tilesky C. Phoanda

Abstract: Endophytic fungi is a species of fungi which grows on plant tissue that are harmless for the host plant it self. Endophytic fungi can produce a substance potencial to be antibacteria. This research aimed to test the presence of antibacterial effect on an isolated root of mangrove plant Bruguiera gymnorrhiza to Escherichia coli and Staphylococcus aureus. The methods that are being used as test to antibacterial was sandwich method, done by putting mycelia of endophytic fungi in agar combination media that has been smear with Escherichia coli and Staphylococcus aureus. Obtained from research that has been made, it conducted two species of endophytic fungi that isolated from mangrove plant root Bruguiera gymnorrhiza. Both endophytic fungi has effect as antibacteria against Escherichia coli and Staphylococcus aureus, but the second type of endophytic fungi has more effective and stronger antibacterial effect compare to endophytic fungi type I and positive control. Keyword : antibacterial, endophytic fungi, mangrove Bruguiera gymnorrhiza.  Abstrak: Jamur endofit adalah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji ada tidaknya efek antibakteri jamur endofit yang diisolasi dari akar tumbuhan bakau Bruguiera gymnorrhiza terhadap bakteri Staphylococcus aureus dan Escherichia coli. Metode yang digunakan untuk uji antibakteri yaitu metode sandwich, dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan diperoleh dua jenis jamur endofit yang diisolasi dari akar tumbuhan bakau Bruguiera gymnorrhiza. Kedua jamur endofit memiliki efek sebagai antibakteri terhadap kedua bakteri uji akan tetapi jamur endofit tipe II memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit tipe I dan kontrol positif. Kata kunci: antibakteri, jamur endofit, bakau Bruguiera gymnorrhiza


2018 ◽  
Vol 9 (4) ◽  
pp. 408-411
Author(s):  
Zineb Hacini ◽  
Fatima Khedja ◽  
Ibrahim Habib ◽  
Zaouia Kendour ◽  
Zineb Debba

The benzoin resin is used extensively in traditional medicine for its many reported therapeutic properties. The essential oils of three different types of benzoin resin were extracted using the traditional method in this study. The yield of essential oils of the white, red and gray types of resin was 1.01, 0.92 and 0.54%, respectively. The obtained extracts were tested against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. The tests showed that essential oil of gray type resin is effective against both Escherichia coli (14 mm) and Staphylococcus aureus (11 mm). The antioxidant activity has been also evaluated to compare the efficiency of different type of resin with DPPH· assay. In the DPPH· system, the antioxidant activity of the red resin extract (0.01 μg/mL) was superior to that of the white (27.32 μg/mL) and gray (42.90 μg/mL) extracts, with IC50 values, respectively.


2020 ◽  
Vol 77 (12) ◽  
pp. 3969-3977
Author(s):  
Raimundo Luiz Silva Pereira ◽  
Thiago Sampaio de Freitas ◽  
Priscilla Ramos Freitas ◽  
Ana Carolina Justino de Araújo ◽  
Fábia Ferreira Campina ◽  
...  

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Yugo Berri Putra Rio ◽  
Aziz Djamal ◽  
Asterina Asterina

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus


2019 ◽  
Vol 11 ◽  
pp. 1-12
Author(s):  
Larissa de Oliveira ◽  
Sabrina de Souza Sales

Syzygium aromaticum L. and Ocimum gratissimum L. essential oils were tested for their effectiveness in reduction the counts of inoculated Staphylococcus aureus and Escherichia coli in sheepmeat. The inhibitory effect was analysed by disk diffusion and broth macrodilution method with four strains and seven concentrations. Disinfectant activity of the oils was assessed using the suspension test with two strains and two concentrations. The inhibition was observed at concentrations 400, 200 and 100 μL/mL. Syzygium aromaticum L. oil was better than Ocimum gratissimum L. against bacteria isolated from sheep carcasses. The minimum bactericidal concentration of the essential oils aginst inocula ranged from 50 to 200 μL/mL. The suspension test showed that after 5 minutes of contact, Syzygium aromaticum L. disinfectant at 400 μL/mL deactivated the inoculants completely. The results demonstrated that essential oils exerted a significant bactericidal and bacteriostatic action against pathogens.


2020 ◽  
Vol 9 (11) ◽  
pp. e4239118504
Author(s):  
Maysa Mathias Alves Pereira ◽  
Ludmila Caproni Morais ◽  
Natália Jubram Zeneratto ◽  
Willian de Souza Matias Reis ◽  
Omar Cabezas Gómez ◽  
...  

The objective this stud was to evaluate the antimicrobial activity of essential oils (organic vs. conventional) of Origanum vulgare L. in the action against Candida albicans, Escherichia coli and Staphylococcus aureus. The inoculation of rhizobacteria (environmental (ME) and Bacillus subtilis) potentiated the antimicrobial action.  The essential oils of Traditional (organic) cultivation presented antibacterial action and antifungal, while the essential oil of conventional cultivation (NPK) showed no any antibacterial or antifungal activity. It was concluded that the formulation of vermicomposting and the use of rhizobacteria are potential technologies and tools for family farmers and traditional community in the cultivation of orégano.


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