scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDOFIT AKAR TUMBUHAN BAKAU (Bruguiera gymnorrhiza) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Tilesky C. Phoanda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
Plant Root ◽  
Positive Control ◽  
Type I ◽  
Bruguiera Gymnorrhiza ◽  
Mangrove Plant ◽  
And Staphylococcus Aureus

Abstract: Endophytic fungi is a species of fungi which grows on plant tissue that are harmless for the host plant it self. Endophytic fungi can produce a substance potencial to be antibacteria. This research aimed to test the presence of antibacterial effect on an isolated root of mangrove plant Bruguiera gymnorrhiza to Escherichia coli and Staphylococcus aureus. The methods that are being used as test to antibacterial was sandwich method, done by putting mycelia of endophytic fungi in agar combination media that has been smear with Escherichia coli and Staphylococcus aureus. Obtained from research that has been made, it conducted two species of endophytic fungi that isolated from mangrove plant root Bruguiera gymnorrhiza. Both endophytic fungi has effect as antibacteria against Escherichia coli and Staphylococcus aureus, but the second type of endophytic fungi has more effective and stronger antibacterial effect compare to endophytic fungi type I and positive control. Keyword : antibacterial, endophytic fungi, mangrove Bruguiera gymnorrhiza.  Abstrak: Jamur endofit adalah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji ada tidaknya efek antibakteri jamur endofit yang diisolasi dari akar tumbuhan bakau Bruguiera gymnorrhiza terhadap bakteri Staphylococcus aureus dan Escherichia coli. Metode yang digunakan untuk uji antibakteri yaitu metode sandwich, dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan diperoleh dua jenis jamur endofit yang diisolasi dari akar tumbuhan bakau Bruguiera gymnorrhiza. Kedua jamur endofit memiliki efek sebagai antibakteri terhadap kedua bakteri uji akan tetapi jamur endofit tipe II memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit tipe I dan kontrol positif. Kata kunci: antibakteri, jamur endofit, bakau Bruguiera gymnorrhiza

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDIFIT PADA DAUN MANGROVE Sonneratia alba TERHADAP BAKTERI UJI Staphylococcus aureus DAN Escherichia coli

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwilestari . ◽  
Henoch Awaloei ◽  
Jimmy Posangi ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
Type I ◽  
Mangrove Plant ◽  
Activity Test ◽  
Sonneratia Alba ◽  
Tissue System

Abstract: Endophytic fungi is a species of fungi which life on plant tissue system. Endophytic fungi can be isolated from the roots, stems and leave of the plant. Endophytic fungi can produce a substance potencial to be antibacteria. This research aimed to test the presence of antibacterial effect on an isolated leaf of mangrove plant Sonneratia alba towards Staphylococcus aureus and Escherichia coli. The method that is used for the antibacterial activity test, done by putting mycelia of endophytic fungi in combination media that has been smeared with Staphylococcus aureus and Escherichia coli. Obtained from research that has been made, it conducted two species of endophytic fungi that isolated from mangrove plant leaf Sonneratia alba. Both endophytic fungi has effect as antibacteria against Staphylococcus aureus and Escherichia coli, but the second type of endophytic fungi has more effective and stronger antibacterial effect compare to endophytic fungi type I and positive control.Keywords: antibacterial, endophytic fungi, mangrove Sonneratia albaAbstrak: Jamur endofit adalah jamur yang terdapat dalam sistem jaringan tumbuhan. Jamur endofit dapat diisolasi dari akar, batang dan daun tumbuhan. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antibakteri jamur endofit daun Sonneratia alba terhadap bakteri Staphylococcus aureus dan Escherichia coli. Metode yang digunakan untuk uji antibakteri dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan diperoleh dua jenis jamur endofit yang diisolasi dari daun tumbuhan mangrove Sonneratia alba. Kedua jamur endofit memiliki efek sebagai antibakteri terhadap kedua bakteri uji akan tetapi jamur endofit tipe II memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit tipe I dan kontrol positif.Kata kunci: antibakteri, jamur endofit, daun Sonneratia alba

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT PADA DAUN DAN BATANG TUMBUHAN KUMIS KUCING (Orthosiphon aristatus) TERHADAP BAKTERI Escherichia coli DAN staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Excel Pangouw ◽  
Jimmy Posangi ◽  
Widya Astuty Lolo ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
E Coli ◽  
Average Value ◽  
Escherichia Coli Bacteria ◽  
Cat’S Whiskers ◽  
And Staphylococcus Aureus

ABSTRACTEndophytic fungi are fungi that live in plant tissues and do not harm the plants. Endophytic fungi can produce compounds that have the potential to be antibacterial. This study aims to examine the antibacterial activity of endophytic fungi isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) against Escherichia coli and Staphylococcus aureus bacteria. Cat's whiskers contain essential oils which are used as antibacterial against some pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The method used is experimentally in the laboratory to test the antibacterial activity of endophytic fungi isolates derived from cat's whiskers (Orthosiphon aristatus) on Escherichia coli and Staphylococcus aureus bacteria using the hole / well method. In the research results obtained four samples of endophytic fungi extract isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) . The results of antibacterial research showed that endophytic mushroom extract isolated from cat's whiskers were more effective in inhibiting Escherichia coli bacteria compared to Staphylococcus aureus. In Escherichia coli bacteria the average value of inhibition is 5 mm whereas in Staphylococcus aureus bacteria the average value of inhibition is only 2 mm. So it can be seen that the endophytic fungus from the leaves of cat's whiskers has a better antibacterial effect than the endophytic fungus from the stems of the cat's whiskets. Conclusion, etidophytic fungi isolated from leaves and stems of cat's whiskers (Orthosiphon aristatus) have an antibacterial effect on the growth of E. coli bacteria and are not effective against S. aureus bacteria.Keywords: Cat's whiskers (Orthasiphan aristatus), antibacterial activity, Escherichia coli and Staphylococcus aureus. ABSTRAKJamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri jamur endofit yang diisolasi dari daun dan batang tumbuhan kumis kucing (Orthosiphon aristatus) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Kumis kucing mengandung minyak atsiri yang digunakan sebagai antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Metode yang dipakai yakni secara eksperimen di laboratorium untuk menguji adanya aktivitas antibakteri dari isolat jamur endofit  yang diberasal dari tumbuhan kumis kucing (Orthosiphon aristatus)  pada bakteri  Escherichia coli dan Staphylococcus aureus dengan menggunakan metode lubang/sumuran. Pada hasil penelitan didapatkan empat sampel ekstrak jamur endofit yang diisolasi dari daun dan batang  tumbuhan kumis kucing (Orthosiphon aristatus). Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan kumis kucing lebih efektif menghambat  bakteri Escherichia coli dibandingkan dengan bakteri Staphylococcus aureus. Pada bakteri  Escherichia coli nilai rata-rata daya hambat yaitu 5 mm sedangkan pada bakteri Staphylococcus aureus nilai rata-rata daya hambat yaitu hanya 2 mm. Sehingga dapat diketahui bahwa jamur endofit dari daun kumis kucing memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari batang kumis kucing. Kesimpulan, Jamur endofit yang diisolasi dari daun  dan batang kumis kucing (Orthosiphon aristatus) memiliki efek antibakteri terhadap pertumbuhan bakteri E. coli dan tidak efektiv terhadap bakteri S. aureus.Kata Kunci:  Tumbuhan kumis kucing (Orthosiphon aristatus), aktivitas antibakteri, Escherichia coli dan Staphylococcu saureus.

scholarly journals Uji Bioaktivitas Antibakteri Senyawa murni dari Jamur Endofit Sporothrix sp Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Dewi Yudiana Shinta ◽  
Yusmarini Yusmarini ◽  
Herix Sonata MS ◽  
Hilwan Yuda Teruna ◽  
Saryono Saryono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Positive Control ◽  
Significant Inhibition ◽  
New Drugs ◽  
Diffusion Method ◽  
Significant Difference ◽  
Pure Compounds ◽  
Escherichia Coli Bacteria

Modern medicines that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi found obstacles in the amount of pure compounds produced. Therefore further research is needed by using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by Sporothrix sp endophytic fungi from Dahlia tuber (Dahlia variabilis). Test the activity of pure compounds produced by Sporothrix sp. Endophytic fungi on E. coli and Staphylococcus aureus determined by disc diffusion method. With doses of 10, 30 and 50μg/disk. In Escherichia coli bacteria doses 10 and 50μg/disk gave significant inhibition of pure compounds from isolation compared to the positive control of ciprofloxacin, which was marked by a statistically significant test result (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in doses of both doses of 10.30 and 50μg/disk. Determination of pure compounds was carried out by HPLC and Infra Red Spectrophotometry.

Preparation and Antibacterial Effect of Nanopalygorskite Supported Nano-Silver Powder

2014 ◽  
Vol 1052 ◽  
pp. 327-331 ◽  
Author(s):  
Jian Rong Wang ◽  
Ya Li Liu ◽  
Bin Liu ◽  
Yan Ping Wu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Room Temperature ◽  
Antibacterial Effect ◽  
Silver Powder ◽  
Nano Silver ◽  
Good Antibacterial Activity ◽  
Novel Method ◽  
And Staphylococcus Aureus

A novel method was used to make palygorskite supported nano-silver powder at room temperature. XRD result showed the presence of silver in the final product. TEM investigation revealed that nano-sliver particles of 6-10 nm in diameter were successfully attached on surface of palygorskite fiber. FTIR was also used to clarify the formation mechanism of our method. The antibacterial effect of palygorskite supported nano-silver powder was investigated. Palygorskite supported nano-silver powder had good antibacterial activity on standard Escherichia coli and Staphylococcus aureus.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI ISOLAT CENDAWAN ENDOFIT ASAL TUMBUHAN SENDUDUK (Melastoma malabathricum L.) TERHADAP Escherichia coli DAN Staphylococcus aureus

2018 ◽  
Vol 8 (2) ◽  
pp. 7-12
Author(s):  
Israwati Harahap ◽  
Vivin Paddillah Rahmi ◽  
Nofripa Herlina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Zone Of Inhibition ◽  
Melastoma Malabathricum ◽  
Symbiotic Microorganism ◽  
And Staphylococcus Aureus ◽  
Test Result

Endophytic fungi is a symbiotic microorganism which live inside plant tissues and not harm to their host. Several genera from endophytic fungi known to produce secondary metabolite compounds like antibiotics, anticancer, antifungal, antiviral and antimalarial. This study aimed to investigate antibacterial activity of endophytic fungi from senduduk (Melastoma malabathricum L.) against Escherichia coli and Staphylococcus aureus. Based on the test result, 11 isolates endophytic fungi from senduduk were known to have antibacterial activities. Isolate code 23 have ability to inhibit the growth of E.coli with diameters zone of inhibition is 22 mm and eight isolate endophytic fungi have ability to inhibit the growth of S.aureus (showed by inhibition zone).

scholarly journals Uji efek antibakteri daun mangrove Rhizophora apiculata terhadap bakteri Pseudomonas aeruginosa dan Staphylococcus aureus

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Vivi P. Santoso ◽  
Jimmy Posangi ◽  
Henoch Awaloei ◽  
Robert Bara
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Endophytic Fungi ◽  
Bacterial Growth ◽  
Antibacterial Effect ◽  
Experimental Methods ◽  
Rhizophora Apiculata ◽  
Tissue Systems ◽  
Bacterial Growth Inhibition ◽  
And Staphylococcus Aureus

Abstract: Endophytic Fungi are microorganisms that live in the plant tissue systems such as seeds, leaves, flowers, twigs, stems, and roots. Endophytic fungi used in this study was isolated from Mangrove Rhizophora apiculata leaves. R. apiculata is widely grown in coastal areas. This study aimed to determine whether there was an antibacterial effect of endophytic fungi that was isolated from leaves of Rhizophora apiculata against the sample bacteria Pseudomonas aeruginosa and Staphylococcus aureus. This study was conducted in the Pharmacology and Therapeutics Laboratory of Sam Ratulangi University by using experimental methods. The results showed two types of fungi, tusky white endophytic fungi and white filamentous endophytic fungi, isolated from Mangrove Rhizophora apiculata leaves. Both types of fungi showed the presence of bacterial growth inhibition, but the white filamentous endophytic fungi had greater antibacterial effect than the tusky white endophytic fungi.Keywords: endophytic fungi, antibacterial, rhizophora apiculata leavesAbstrak: Jamur endofit merupakan mikroorganisme yang hidup di dalam sistem jaringan tumbuhan seperti biji, daun, bunga, ranting, batang, dan akar. Jamur endofit yang digunakan pada penelitian ini merupakan hasil isolasi dari daun mangrove Rhizophora apiculata. Tanaman mangrove R.apiculata ini banyak tumbuh di kawasan pesisir pantai. Penelitian ini bertujuan untuk mengetahui apakah terdapat efek antibakteri jamur endofit yang diisolasi dari daun Rhizophora apiculata terhadap bakteri uji Pseudomonas aeruginosa dan Staphylococcus aureus. Penelitian ini dilakukan di Laboratorium Farmakologi dan Terapi Universitas Sam Ratulangi Manado dengan menggunakan metode eksperimental. Hasil penelitian ini didapatkan dua jenis jamur yaitu jamur endofit berwarna putih gading dan jamur endofit berwarna putih berserabut yang diisolasi dari daun mangrove Rhizophora apiculata. Kedua jenis jamur menunjukan adanya daya hambat pertumbuhan kedua bakteri uji, namun jamur endofit berwarna putih berserabut memiliki efek antibakteri lebih besar dari pada jamur endofit berwarna putih gading.Kata kunci: jamur endofit, antibakteri, daun rhizophora apiculata

scholarly journals Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Michele Sumampouw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Potato Dextrose Agar ◽  
Rhizophora Stylosa ◽  
E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli.   Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua  jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan  zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT AKAR BAKAU Avicennia marina TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Firdy Liwang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Avicennia Marina ◽  
Positive Control ◽  
Potato Dextrose Agar ◽  
Fungal Mycelium ◽  
Tidal Zone ◽  
E Coli

Abstract: In this his study we used endophytic fungi isolated from the roots of mangrove Avicennia marina growing on tidal zone around Tasik Ria Minahasa, North Sulawesi. The fungi were isolated and then tested the antibacterial effect against Staphylococcus aureus and Escherichia coli. Potato Dextrose agar was used in order to isolate the target fungi. The fungi began to grow on the second day after inoculation. Differentiation and purification processes to isolate the fungus obtained by observing fungi mycelia. While, the antibacterial activity test was following agar diffusion assay by planting 10 mm diameter of fungal mycelium together with particular bacteria onto the combination media. Ciprofloxacin was used as a positive control. The results shows a visible bacterial inhibition zone around the growing fungi with a diameter between 18 mm to 20.4 mm. Based on the results, it can be concluded that endophytic fungi isolated from the roots of mangrove Avicennia marina is able to inhibit the growth of S.aureus and E. coli. Keyword: Avicennia marina, Antibacterial, endophytic fungi   Abstrak: Penelitian ini menggunakan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina yang tumbuh di perairan sekitar Tasik Ria Minahasa, Sulawesi Utara. Jamur endofit yang diisolasi kemudian diuji efek antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolasi jamur endofit menggunakan media PDA (Potato Dextrose Agar). Jamur mulai tumbuh pada hari kedua setelah inokulasi. Proses pemurnian jamur diperoleh dengan mengisolasi jamur dengan membedakan miselia. Pengujian efek antibakteri mengikuti prosedur agar difusi dengan menanam miselium jamur sebesar 10 mm pada media kombinasi yang telah diolesi bakteri uji. Sebagai kontrol positif digunakan Ciprofloxacin. Hasil penelitian terlihat diameter zona hambat pertumbuhan bakteri yang terbentuk sebesar 18 mm hingga 20,4 mm. Berdasarkan hasil penelitian maka dapat disimpulkan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina mampu menghambat pertumbuhan bakteri S. aureus dan bakteri E. coli. Kata kunci: Avicennia marina, antibakteri, jamur endofit

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