Antibiofilm activity of Cutibacterium acnes cell-free conditioned media against Staphylococcus spp.

Objective: The aim of this study was to identify the slime production and evaluate the effects of Rosmarinus officinalis (rosemary) and Syzygium cumini (jambolan) glycolic extracts, and 0.12% chlorhexidine (CHX) in biofilms formed by strains of coagulase-positive Staphylococcus - CPS and coagulase negative Staphylococcus - CNS isolated from the oral cavity. Material and Methods: Slime production was evaluated by two methods: the color of colony presented in Congo red agar, and through the amount of slime adhered to polystyrene. Biofilms were grown in acrylic resin discs immersed in broth, inoculated with microbial suspension (106 cells/ml) and incubated at 37°C/48 h. After formation, the biofilms were exposed for 5 minutes to glycol extracts, CHX or saline solution. The viability of biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, and analyzed statistically by Tukey test (p <0.05). Results: The strains S. aureus, S. schleiferi and S. epidermidis obtained the highest values of slime adhered to polystyrene. R. officinalis promoted reductions ranging from 12.1% to 78.7% in biofilms formed by isolates of CPS, and 9.2% to 73.7% in the biofilms of CNS. S. cumini reduced 12% to 55.7% in biofilms of CPS, and 7.9% to 71.5% in biofilms of CNS. With exception of S. saprophyticus, glycol extracts produced significant reductions in biofilms. For five isolates studied, R. officinalis produced greater reductions than CHX. Conclusion: R. officinalis and S. cumini showed effective antibiofilm activity against isolates that showed slime production. KeywordsBiofilm; Rosmarinus officinalis; Slime; Staphylococcus; Syzygium cumini.

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Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Antibiotics ◽

10.3390/antibiotics9060279 ◽

2020 ◽

Vol 9 (6) ◽

pp. 279 ◽

Cited By ~ 1

Author(s):

Virginie Lemoine ◽

Clément Bernard ◽

Charlotte Leman-Loubière ◽

Barbara Clément-Larosière ◽

Marion Girardot ◽

...

Keyword(s):

Candida Albicans ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Single Species ◽

Arthrospira Platensis ◽

Antibiofilm Activity ◽

Interspecies Interactions ◽

Cutibacterium Acnes ◽

Additional Protection ◽

Objective View

Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria Arthrospira platensis, rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarriers, were studied on single- and dual-species biofilms of Candida albicans and Cutibacterium acnes. Their ability to inhibit the biofilm formation and to eradicate 24 h old biofilms was investigated. Concentrations of each species were evaluated using flow cytometry. Extracts prevented the growth of C. acnes single-species biofilms (inhibition > 75% at 0.2 mg/mL) but failed to inhibit preformed biofilms. Nanovectorised extracts reduced the growth of single-species C. albicans biofilms (inhibition > 43% at 0.2 mg/mL) while free extracts were weakly or not active. Nanovectorised extracts also inhibited preformed C. albicans biofilms by 55% to 77%, whereas the corresponding free extracts were not active. In conclusion, even if the studied nanocarrier systems displayed promising activity, especially against C. albicans, their efficacy against dual-species biofilms was limited. This study highlighted that working in such polymicrobial conditions can give a more objective view of the relevance of antibiofilm strategies by taking into account interspecies interactions that can offer additional protection to microbes.

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Effect of different agents with potential antibiofilm activity on antimicrobial susceptibility of biofilms formed by Staphylococcus spp. isolated from implant-related infections

The Journal of Antibiotics ◽

10.1038/ja.2016.9 ◽

2016 ◽

Vol 69 (9) ◽

pp. 686-688 ◽

Cited By ~ 5

Author(s):

Diana Molina-Manso ◽

Gema Del-Prado ◽

Enrique Gómez-Barrena ◽

Jose Cordero-Ampuero ◽

Ricardo Fernandez-Roblas ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Antibiofilm Activity ◽

Staphylococcus Spp

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Biological Efficacy Evaluation of a Non-Cross-Linked Hyaluronic Acid Dermal Filler for Biomedical Application in Inflammatory Scalp Conditions

Life ◽

10.3390/life12010002 ◽

2021 ◽

Vol 12 (1) ◽

pp. 2

Author(s):

Sabrina Sommatis ◽

Maria Chiara Capillo ◽

Elsa Liga ◽

Cristina Maccario ◽

Raffaele Rauso ◽

...

Keyword(s):

Biomedical Application ◽

Inflammatory Marker ◽

Trigger Factor ◽

Inflammatory Activity ◽

Dermal Filler ◽

Antibiofilm Activity ◽

Efficacy Evaluation ◽

Cutibacterium Acnes ◽

Efficacy Assessment ◽

Anti Inflammatory

(1) Background: The dysbiosis of some cutaneous commensal microorganisms is the trigger factor for the activation of the inflammatory cascade by keratinocytes in many skin disorders. Mesotherapy is an innovative technique for many scalp disorders, with the function of restoring the physiology of the skin. (2) Methods: the antimicrobial, antibiofilm and anti-inflammatory activity of the non-cross-linked HA formulation (Hydro Deluxe, Matex Lab S.p.a., Brindisi, Italy) was investigated against the most common microorganisms of the scalp (Staphyloccoccus epidermis, Staphyloccoccus aureus, Cutibacterium acnes and Malassezia furfur). Anti-inflammatory activity was evaluated on an internal 3D model of Reconstructed Human Epidermis (RHE) inserts infected with the strains as pro-inflammatory stimulus. (3) Results and Conclusions: the data collected showed a good antimicrobial and antibiofilm activity against all selected strains. The HA-based formulation did not show cytotoxicity on RHE, either alone or in presence of the infectious stimulus. The analysis of the expression of Interleukin (IL)-8 levels showed an excellent ability to reduce this pro-inflammatory marker. Overall, the efficacy assessment of the formulation supported its potential effectiveness in mesotherapy for the treatment of scalp disorders.

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Wundinfektionen durch methicillinresistente Staphylococcus spp. (MRS) bei Kleintieren und Pferden: klinische Bedeutung, Therapie und Prophylaxe

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/s-0038-1622712 ◽

2008 ◽

Vol 36 (S 01) ◽

pp. S5-S10

Author(s):

B. Walther ◽

A. Lübke-Becker ◽

L. H. Wieler

Keyword(s):

Klinische Relevanz ◽

Klinische Bedeutung ◽

Staphylococcus Spp

Zusammenfassung:In den vergangenen Jahrzehnten erlangten verschiedene Populationen von Staphylococcus sp. multiple Resistenzen durch die Akkumulation vielfältiger Resistenzdeterminanten. Heutzutage besitzen Infektionen durch methicillinresistente Staphylococcus sp. (MRS) weltweit sowohl in der Humanmedizin als auch in zunehmendem Maße in Veterinärmedizin große klinische Relevanz. Seit den frühen siebziger Jahren sind MRS-Infektionen bei Tieren bekannt. In den letzten Jahren kam es jedoch zu einem deutlichen Anstieg derartiger Berichte. Insbesondere bei Kleintieren und Pferden treten Infektionen durch MRS auf, und zwar vorrangig in Zusammenhang mit nosokomialen Wundinfektionen. Die eindeutige Diagnose einer Infektion mit MRS bereitet zwar heute keine Probleme mehr, doch sind einige wesentliche Punkte zu beachten, weshalb nur erfahrene Diagnostiker mit dieser Thematik beauftragt werden sollten. Die Therapie erfolgt nach Auswertung des Antibiogramms (unabhängig vom Antibiogramm sind alle β-Lactame als resistent einzustufen) und wenn möglich lokal. Auch die Frage nach dem Zoonosecharakter animaler MRS und den damit verbunden Problembereichen (Hygienemanagement, Haftungsfragen, Sorgfaltspflichten, Einsatz humaner Reservewirkstoffe) tritt zunehmend in den Fokus der öffentlichen Wahrnehmung.

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Wundinfektionen durch methicillinresistente Staphylococcus spp. (MRS) bei Kleintieren und Pferden: klinische Bedeutung, Therapie und Prophylaxe

Tierärztliche Praxis Ausgabe G Großtiere / Nutztiere ◽

10.1055/s-0038-1624560 ◽

2008 ◽

Vol 36 (S 01) ◽

pp. S05-S10

Author(s):

A. Lübke-Becker ◽

L. Wieler ◽

B. Walther

Keyword(s):

Klinische Relevanz ◽

Klinische Bedeutung ◽

Staphylococcus Spp

ZusammenfassungIn den vergangenen Jahrzehnten erlangten verschiedene Populationen von Staphylococcus sp. multiple Resistenzen durch die Akkumulation vielfältiger Resistenzdeterminanten. Heutzutage besitzen Infektionen durch methicillinresistente Staphylococcus sp. (MRS) weltweit sowohl in der Humanmedizin als auch in zunehmendem Maße in Veterinärmedizin große klinische Relevanz. Seitden frühen siebziger Jahren sind MRS-Infektionen bei Tieren bekannt.Inden letzten Jahren kam es jedoch zu einem deutlichen Anstieg derartiger Berichte. Insbesondere bei Kleintieren und Pferden treten Infektionen durch MRSauf,und zwar vorrangig in Zusammenhang mit nosokomialen Wundinfektionen. Die eindeutige Diagnose einer Infektion mit MRS bereitet zwar heute keine Probleme mehr,doch sind einige wesentliche Punkte zu beachten, weshalb nur erfahrene Diagnostiker mit dieser Thematik beauftragt werden sollten. Die Therapie erfolgt nach Auswertung des Antibiogramms (unabhängigvom Antibiogramm sindalle β-Lactame als resistent einzustufen) und wenn möglich lokal. Auch die Frage nach dem ZoonosecharakteranimalerMRS undden damit verbunden Problembereichen (Hygienemanagement, Haftungsfragen, Sorgfaltspflichten, Einsatz humaner Reservewirkstoffe) tritt zunehmend in den Fokus der öffentlichen Wahrnehmung.

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