Increased dental caries activity in pre-school children with low baseline levels of serum IgG antibodies against the bacterial species Streptococcus mutans

1987 ◽  
Vol 32 (1) ◽  
pp. 55-60 ◽  
Author(s):  
A.S. Aaltonen ◽  
J. Tenovuo ◽  
O.-P. Lehtonen
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
School Children ◽  
Bacterial Species ◽  
Igg Antibodies ◽  
Caries Activity ◽  
Serum Igg ◽  
Baseline Levels

scholarly journals Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children

2005 ◽  
Vol 54 (7) ◽  
pp. 661-665 ◽  
Author(s):  
Mitsugi Okada ◽  
Yoshiko Soda ◽  
Fumiko Hayashi ◽  
Takako Doi ◽  
Junji Suzuki ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
School Children ◽  
Streptococcus Sobrinus

scholarly journals NagR Differentially Regulates the Expression of theglmSandnagABGenes Required for Amino Sugar Metabolism by Streptococcus mutans

2015 ◽  
Vol 197 (22) ◽  
pp. 3533-3544 ◽  
Author(s):  
Lin Zeng ◽  
Robert A. Burne
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
Random Mutagenesis ◽  
Sugar Metabolism ◽  
Amino Sugar ◽  
Binding Domain ◽  
Promoter Regions ◽  
Content Type ◽  
Effector Binding

ABSTRACTThe ability of bacteria to metabolize glucosamine (GlcN) andN-acetyl-d-glucosamine (GlcNAc) is considered important for persistent colonization of the oral cavity. In the dental caries pathogenStreptococcus mutans, the NagR protein regulates the expression ofglmS, which encodes a GlcN-6-P synthetase, andnagA(GlcNAc-6-P deacetylase) andnagB(GlcN-6-P deaminase), which are required for the catabolism of GlcNAc and GlcN. Two NagR-binding sites (dre) were identified in each of the promoter regions fornagBandglmS. Using promoter-reporter gene fusions, the role of eachdresite was examined in the regulation ofglmSandnagBpromoter activities in cells grown with glucose, GlcNAc, or GlcN. A synergistic relationship between the twodresites in theglmSpromoter that required proper spacing was observed, but that was not the case fornagB. Binding of purified NagR to DNA fragments from both promoter regions, as well as todresites alone, was strongly influenced by particular sugar phosphates. Using a random mutagenesis approach that targeted the effector-binding domain of NagR, mutants that displayed aberrant regulation of both theglmSandnagABgenes were identified. Collectively, these findings provide evidence that NagR is essential for regulation of genes for both the synthesis and catabolism of GlcN and GlcNAc inS. mutans, and that NagR engages differently with the target promoter regions in response to specific metabolites interacting with the effector-binding domain of NagR.IMPORTANCEGlucosamine andN-acetylglucosamine are among the most abundant naturally occurring sugars on the planet, and they are catabolized by many bacterial species as sources of carbon and nitrogen. Representing a group called lactic acid bacteria (LAB), the human dental caries pathogenStreptococcus mutansis shown to differ from known paradigm organisms in that it possesses a GntR/HutC-type regulator, NagR, that is required for the regulation of both catabolism of GlcN and biosynthesis. Results reported here reveal a simple and elegant mechanism whereby NagR differentially regulates two opposing biological processes by surveying metabolic intermediates. This study provides insights that may contribute to the development of novel therapeutic tools to combat dental caries and other infectious diseases.

scholarly journals QPCR Screening of Pediatric Saliva Samples to Evaluate Effects of Dental Sealants on Cariogenic Bacteria Streptococcus Mutans and Scardovia Wiggsiae

2021 ◽  
Vol 2 (1) ◽  
pp. 14-20
Author(s):  
Olivia Tsang ◽  
Kevin Major ◽  
Sonia Santoyo ◽  
Karl Kingsley ◽  
Linh Nguyen
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
Conclusive Evidence ◽  
Oral Microbiota ◽  
Average Amount ◽  
Fluoride Treatment ◽  
Dental Sealants ◽  
Exact Effect ◽  
The Impact

Dental caries is an alarmingly prevalent multifactorial disease worldwide but is most common as a chronic disease among children and adolescents. Within the oral cavity there are hundreds bacterial species, including specific cariogenic pathogens such as Streptococcus mutans and Scardoviawiggsiae. Although dental caries are highly prevalent, it may also be preventable using methods such as dental sealants or fluoride treatment, which have proven to be highly effective in reducing caries risk – although there is limited evidence on the exact effect of these treatments on S. wiggsiaeinparticular. Due to the lack of conclusive evidence, this study is aimed to evaluate the microbial burden of S. mutans and S. wiggsiae among pediatric saliva samples to analyze the effects dental sealants have on these oral microbiota. Saliva samples derived from pediatric patients pre- and post-sealant were obtained from an existing repository. DNA was extracted and screened using qPCR and primers specific for the cariogenic organisms, S. mutans (SM) and S. wiggsiae (SW). These data demonstrated an overall reduction in SW among Post-sealant samples (11.14 U/uL) compared with Pre-sealant samples (11.68 U/uL), as well as a reduction in the overall number of samples harboring S. wiggsiae (n=8). The Pre-sealant average amount of SM (14.54 U/uL) was slightly increased among the Post-sealant samples (15.67 U/uL) with half of the samples analyzed (n=13) exhibiting slight increases in SM levels, and nearly one-third (n=8) exhibiting slight decreases in SM levels. The data from this study may suggest that dental sealants have an impact on certain cariogenic pathogens in the oral microbiota. Whether or not the impact is positive or not in light of the increase in S. mutans remains an observation that should be further analyzed. More research is required to address the limitations found in this study in order to localize the exact effect on the teeth with dental sealants.

scholarly journals Association between Socioeconomic Status, Salivary Streptococcus mutans, Lactobacilli and Dental Caries among 12-Year-Old School Children in Belgaum City

2011 ◽  
Vol 2 (4) ◽  
pp. 316-320
Author(s):  
Mamata Iranna Hebbal ◽  
Sharada C Metgud
Keyword(s):  
Socioeconomic Status ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
School Children ◽  
Colony Count ◽  
Microbial Count ◽  
Treatment Needs ◽  
Chi Square ◽  
Number Of Teeth ◽  
Chi Square Test

ABSTRACT Introduction Aim of the study was to assess dental caries using Modified Dentition Status and Treatment Needs and find its association with salivary Streptococcus mutans and lactobacilli counts among 12-year-old school children of Belgaum city. Materials and methods A self-designed, 13 item, open-ended questionnaire given to parents. Children were examined for dental caries using Modified Dentition Status and Treatment Needs. Stimulated saliva was collected and incubated to estimate lactobacilli and Streptococcus mutans colonies. Chi-square test was used to find the association between variables. Results When DMFT was recorded it was found that 23% of children were free from dental caries, whereas, only 5% of them were free of dental caries when examined using modified criteria. Most of the children had higher microbial count with 72% and 62% of them having > 105 cfu/μl of Streptococcus mutans and lactobacilli colony count respectively. Modified DMFT was significantly associated with frequency of brushing, lactobacilli and Streptococcus mutans counts (p < 0.05). Conclusion The proportion of subjects with dental caries was relatively high in all groups but the number of teeth affected by caries was low. Inclusion of noncavitated incipient lesions could increase sensitivity of index.

scholarly journals THE EFFECTIVENESS OF PROPOLIS FLUORIDE APPLICATION IN INHIBITING DENTAL CARIES ACTIVITY IN SCHOOL CHILDREN AGE 6-9 YEARS OLD

2018 ◽  
Vol 9 ◽  
pp. 1 ◽  
Author(s):  
Risqa Rina Darwita ◽  
Andalina Rhukul Finisha ◽  
Hardiati Nur Wahyuni ◽  
Salsabila Ghina ◽  
Rajiv Muhammad ◽  
...  
Keyword(s):  
Dental Caries ◽  
Primary School ◽  
Topical Application ◽  
School Children ◽  
Tooth Brushing ◽  
Tooth Surface ◽  
Caries Lesion ◽  
Caries Activity ◽  
Tooth Surfaces ◽  
Black Discoloration

Objective: To obtain the effectiveness of propolis fluoride application in inhibiting dental caries activity. Methods: The study was included 170 children aged 6–9 years old with active dentinal carries surface was 532 tooth surfaces, all of 532 tooth surface will applicate use propolis fluoride in school children at primary school SD Kukusan. Evaluation of the amount of dentinal caries lesion that has been arrested was conducted after 1 month, 2 months, and 4 months after topical application. Results: After propolis fluoride applicated shows, that the activated process caries of dentinal caries lesion after 1 month was found in 104 tooth surfaces, 2 months were 115 tooth surfaces, and after 4 months evaluated were found significant increase to be 226 tooth surfaces on dentinal caries activated from 2nd to 4th month after application propolis fluoride (p<0.01). The percentages of arrested caries in, respectively, for 1st month after the application is 99.80%, 2nd month application is 78.32%, and in the 4th month is 57.52% while the score plaque of school children was decline significantly until 36.5% (p<0.05) after 4 months intervention by tooth brushing every week at school. Conclusion: The application of propolis fluoride is effective in inhibiting dentinal caries lesion activity, has the advantage of making no black discoloration to the teeth.

scholarly journals Applications of green tea in dentistry

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 392-395
Author(s):  
Reethega L ◽  
Balaji Ganesh S ◽  
Geetha R V
Keyword(s):  
Side Effects ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Green Tea ◽  
Porphyromonas Gingivalis ◽  
Periodontal Diseases ◽  
Bacterial Species ◽  
Black Tea ◽  
Tea Leaves ◽  
The World

Tea is one of the most consumed beverages in the world. Majority of people consume black tea more than green tea. Green tea is prepared from the dry leaves of Camellia sinensis bush. The extent of oxidation of the leaves determines the type of tea prepared. Green tea is made from the un-oxidised tea leaves and is a type of less processed tea. Therefore, it contains the most number of antioxidants and beneficial phenols. These polyphenols prevent the acid production of bacterial species like Streptococcus mutans and Porphyromonas gingivalis, which are solely involved in the development of dental caries and periodontal diseases. Around 30 articles from Pubmed and other Scopus indexed journals were collected and the data was extracted from them. The articles were selected based on their relevance to the topic and characteristics. The review methods, applications of green tea in various systems, applications of green tea in dentistry and their risk and side effects were discussed. The above topics were discussed accurately and the data was selected accordingly. The topic was discussed to see whether green tea can be used in dentistry.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

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