A scheme for the interpretation of primary and secondary disturbances of plasma and urinary amino acid profiles. A possible way to an expert system

1995 ◽  
Vol 235 (1) ◽  
pp. 1-10 ◽  
Author(s):  
P. Parvy ◽  
J. Bardet ◽  
D. Rabier ◽  
P. Kamoun
Keyword(s):  
Amino Acid ◽  
Expert System ◽  
Amino Acid Profiles ◽  
Urinary Amino

Neutral aminoaciduria in cystathionine β-synthase-deficient mice, an animal model of homocystinuria

2014 ◽  
Vol 306 (12) ◽  
pp. F1462-F1476 ◽  
Author(s):  
Noriyuki Akahoshi ◽  
Shotaro Kamata ◽  
Masashi Kubota ◽  
Takako Hishiki ◽  
Yoshiko Nagahata ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Serum Levels ◽  
Substrate Affinity ◽  
Neutral Amino Acids ◽  
Amino Acid Profiles ◽  
Marked Accumulation ◽  
Urinary Amino ◽  
Severe Growth ◽  
Deficient Mice

The kidney is one of the major loci for the expression of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CTH). While CBS-deficient ( Cbs−/−) mice display homocysteinemia/methioninemia and severe growth retardation, and rarely survive beyond the first 4 wk, CTH-deficient ( Cth−/−) mice show homocysteinemia/cystathioninemia but develop with no apparent abnormality. This study examined renal amino acid reabsorption in those mice. Although both 2-wk-old Cbs−/− and Cth−/− mice had normal renal architecture, their serum/urinary amino acid profiles largely differed from wild-type mice. The most striking feature was marked accumulation of Met and cystathionine in serum/urine/kidney samples of Cbs−/− and Cth−/− mice, respectively. Levels of some neutral amino acids (Val, Leu, Ile, and Tyr) that were not elevated in Cbs−/− serum were highly elevated in Cbs−/− urine, and urinary excretion of other neutral amino acids (except Met) was much higher than expected from their serum levels, demonstrating neutral aminoaciduria in Cbs−/− (not Cth−/−) mice. Because the bulk of neutral amino acids is absorbed via a B0AT1 transporter and Met has the highest substrate affinity for B0AT1 than other neutral amino acids, hypermethioninemia may cause hyperexcretion of neutral amino acids.

scholarly journals Diagnostic biomarkers for USD via serum and urinary amino acid profiles using a metabolomics-based strategy

2021 ◽  
Author(s):  
Yao Gao ◽  
Sisi Gong ◽  
Xinxin Lin ◽  
Tianwen Zhang ◽  
Beiying Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Early Diagnosis ◽  
Urinary Stone ◽  
Stone Disease ◽  
High Recurrence Rate ◽  
Common Disease ◽  
Diagnostic Biomarkers ◽  
Clinical Biomarkers ◽  
Amino Acid Profiles ◽  
Urinary Amino

Abstract Background: Urinary stone disease (USD) is a common disease with a high prevalence, a high recurrence rate and a lack of effective diagnostic biomarkers.Methods: To explore the potential biomarkers for USD early diagnosis, the serum and urinary amino acid (AA) profiles of 74 USD patients were compared with 35 healthy controls (HC) via gas chromatography coupled with mass spectrometry (GC-MS).Results: Based on the serum and urinary AA profiles analyzed by metabolomic strategy, a group of 5 differentially expressed AAs including Ser, Glu, Asp, Ile and Gly and 7 metabolic pathway disorders in patients were revealed.Conclusions: These serum and urinary AA profiles could provided clinical biomarkers for USD early diagnosis, and also indicated that some specific AA metabolic pathways might play important roles in the pathogenesis of USD.

scholarly journals A Specific Urinary Amino Acid Profile Characterizes People with Kidney Stones

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Aniello Primiano ◽  
Silvia Persichilli ◽  
Pietro Manuel Ferraro ◽  
Riccardo Calvani ◽  
Alessandra Biancolillo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Kidney Stones ◽  
Stone Formation ◽  
Amino Acid Profile ◽  
Stone Formers ◽  
Stone Former ◽  
Amino Acid Profiles ◽  
Definition Of ◽  
Urinary Amino

Background. Urolithiasis is the process of stone formation in the urinary tract. Its etiology is only partly known, and efficient therapeutic approaches are currently lacking. Metabolomics is increasingly used in biomarkers discovery for its ability to identify mediators of relevant (patho)physiological processes. Amino acids may be involved in kidney stone formation. The aim of the present study was to investigate the presence of an amino acid signature in stone former urine through a targeted metabolomic approach. Methods. A panel of 35 amino acids and derivatives was assessed in urines from 15 stone former patients and 12 healthy subjects by UPLC-MS. Partial Least Squares Discriminant Analysis (PLS-DA) was used to define amino acid profiles of cases and controls. Results and Discussion. Our approach led to the definition of a specific amino acid fingerprint in people with kidney stones. A urinary amino acid profile of stone formers was characterized by lower levels of α-aminobutyric acid, asparagine, ethanolamine, isoleucine, methionine, phenylalanine, serine, tryptophan, and valine. Metabolomic analysis may lend insights into the pathophysiology of urolithiasis and allow tracking this prevalent condition over time.

Sex-Related Patterns in the Profiles of Human Urinary Amino Acids

1975 ◽  
Vol 21 (13) ◽  
pp. 1970-1975 ◽  
Author(s):  
Henri Dirren ◽  
Arthur B Robinson ◽  
Linus Pauling
Keyword(s):  
Amino Acids ◽  
High Pressure ◽  
Amino Acid ◽  
Young Women ◽  
Amino Acid Analyzer ◽  
Amino Acid Profiles ◽  
Urinary Amino Acids ◽  
Urinary Amino ◽  
Nonparametric Statistical ◽  
Related Pattern

Abstract The urinary amino acid profiles of 79 young women and 72 young men were studied with an automated high-pressure amino acid analyzer. A nonparametric statistical analysis was applied for detecting profile differences related to sex and other variables. A strong sex-related pattern was confirmed and its power for sex determination was evaluated. For the women, profile differences related to the use of oral contraceptives were also discovered and evaluated. No correlation with the menstrual cycle was detected.

A comparative study of polyphenolic and amino acid profiles of commercial fruit beers

2021 ◽  
Vol 100 ◽  
pp. 103921
Author(s):  
Diana K. Baigts-Allende ◽  
Alexa Pérez-Alva ◽  
Melissa A. Ramírez-Rodrigues ◽  
Adriana Palacios ◽  
Milena M. Ramírez-Rodrigues
Keyword(s):  
Amino Acid ◽  
Comparative Study ◽  
Amino Acid Profiles

scholarly journals Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2033
Author(s):  
Chuleeporn Bungthong ◽  
Sirithon Siriamornpun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Silk Protein ◽  
Extraction Time ◽  
Antioxidant Power ◽  
Silk Cocoon ◽  
Amino Acid Profiles

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

scholarly journals Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1567
Author(s):  
Joanna Moro ◽  
Nadezda Khodorova ◽  
Daniel Tomé ◽  
Claire Gaudichon ◽  
Catherine Tardivel ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dietary Intakes ◽  
Analysis Model ◽  
Factorial Method ◽  
New Methods ◽  
Food And Agriculture ◽  
Food And Agriculture Organization ◽  
Weanling Rats ◽  
Urinary Amino ◽  
One Way Anova

Objective: Dietary intakes must cover protein and essential amino acid (EAA) requirements. For this purpose, different methods have been developed such as the nitrogen balance method, factorial method, or AA tracer studies. However, these methods are either invasive or imprecise, and the Food and Agriculture Organization of the United Nations (FAO, 2013) recommends new methods and, in particular, metabolomics. The aim of this study is to determine total protein/EAA requirement in the plasma and urine of growing rats. Methods: 36 weanling rats were fed with diets containing 3, 5, 8, 12, 15, and 20% protein for 3 weeks. During experimentation, urine was collected using metabolic cages, and blood from the portal vein and vena was taken at the end of the experiment. Metabolomics analyses were performed using LC-MS, and the data were analyzed with a multivariate analysis model, partial least Squares (PLS) regression, and independent component-discriminant analysis (ICDA). Each discriminant metabolite identified by PLS or ICDA was tested by one-way ANOVA to evaluate the effect of diet. Results: PLS and ICDA allowed us to identify discriminating metabolites between different diet groups. Protein deficiency led to an increase in the AA catabolism enzyme systems inducing the production of breakdown metabolites in the plasma and urine. Conclusion: These results indicate that metabolites are specific for the state of EAA deficiency and sufficiency. Some types of biomarkers such as AA degradation metabolites appear to be specific candidates for protein/EAA requirement.

scholarly journals Alteration of serum amino acid profiles by dietary adenine supplementation inhibits fatty liver development in rats

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hiroki Nishi ◽  
Daisuke Yamanaka ◽  
Masato Masuda ◽  
Yuki Goda ◽  
Koichi Ito ◽  
...  
Keyword(s):  
Amino Acid ◽  
Fatty Liver ◽  
Causative Factor ◽  
Amino Acid Profile ◽  
Liver Development ◽  
Concentration Data ◽  
Amino Acid Profiles ◽  
Arginine Deficiency ◽  
Branched Chain ◽  
Tag Accumulation

AbstractStudies on animal models have demonstrated that feeding a low-arginine diet inhibits triacylglycerol (TAG) secretion from the liver, resulting in marked fatty liver development in rats. Here, we first showed that culturing hepatocytes in the medium mimicking the serum amino acid profile of low-arginine diet-fed rats induced TAG accumulation in the cells, indicating that the specific amino acid profile caused TAG accumulation in hepatocytes. Dietary adenine supplementation completely recovered hepatic TAG secretion and abolished hepatic TAG accumulation in rats. A comprehensive non-linear analysis revealed that inhibition of hepatic TAG accumulation by dietary adenine supplementation could be predicted using only serum amino acid concentration data. Comparison of serum amino acid concentrations indicated that histidine, methionine, and branched-chain amino acid (BCAA) concentrations were altered by adenine supplementation. Furthermore, when the serum amino acid profiles of low-arginine diet-fed rats were altered by modifying methionine or BCAA concentrations in their diets, their hepatic TAG accumulation was abolished. Altogether, these results suggest that an increase in methionine and BCAA levels in the serum in response to dietary arginine deficiency is a key causative factor for hepatic TAG accumulation, and dietary adenine supplementation could disrupt this phenomenon by altering serum amino acid profiles.

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