Biocatalytic nanomaterials as an alternative to peroxidase enzymes

ABSTRACT: Studies have been performed to identify the proteins present in canine seminal plasma (SP) and relate them to sperm quality as well as to discover molecular markers of reproductive tract diseases. There is evidence that heparin-binding proteins, zinc-binding proteins, and lactoferrin as well as the matrix metalloproteinase, superoxide dismutase, catalase, and glutathione peroxidase enzymes are associated with canine sperm quality. Other studies indicate that prolactin and enzymes like arginine esterase, acid phosphatase, and alkaline phosphatase could be successfully used as biomarkers of reproductive disorders. Thus, the present literature review aims to address aspects related to proteins of the canine SP, their influence on fertility, and their importance as biomarkers of reproductive disorders.

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Studies of chemical and enzymatic characteristics of Yacon (Smallanthus sonchifolius) and its flours

Food Science and Technology ◽

10.1590/s0101-20612013005000020 ◽

2013 ◽

Vol 33 (1) ◽

pp. 75-83 ◽

Cited By ~ 11

Author(s):

Juciane de Abreu Ribeiro Pereira ◽

Maria de Fátima Píccolo Barcelos ◽

Michel Cardoso De Angelis Pereira ◽

Eric Batista Ferreira

Keyword(s):

Oxalic Acid ◽

Dietary Fiber ◽

Titratable Acidity ◽

Soluble Solids ◽

Average Yield ◽

Total Dietary Fiber ◽

Smallanthus Sonchifolius ◽

Total Titratable Acidity ◽

Wet Weight ◽

Peroxidase Enzymes

Due to the importance of studies on yacon related to health, its in natura pulp, in natura peel, pulp flour, and peel flour were chemically analyzed in terms of its centesimal composition, specific minerals, total dietary fiber and fractions, pH, total soluble solids, total titratable acidity, tannins, oxalic acid, and nitrate. The polyphenoloxidase and peroxidase enzymatic activities were evaluated for in natura pulp and peel only. Yacon pulp and peel flour presented average yield of 7.94% and 10.86%, respectively. The in natura pulp presented a higher moisture and carboydrate content and lower lipid, protein, total dietary fiber, and ash than those of the peel flour. The same pattern was observed for pulp flour when compared to peel flour. The highest tannin, nitrate, and oxalic acid levels were found in the peel flour, 15,304.5 mg.kg-1, 1,578.3 mg.kg-1, and 7,925.0 mg.kg-1 (wet weight), respectively. The polyphenoloxidase and peroxidase enzymes presented higher enzymatic activity in the yacon peel. Based on the results obtained, it can be said that the yacon and its derivatives are important dietary carbohydrate and mineral sources and contain antinutritional substance contents lower than those harmful to health.

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Peroxidase enzymes inhibit osteoclast differentiation and bone resorption

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2016.11.007 ◽

2017 ◽

Vol 440 ◽

pp. 8-15 ◽

Cited By ~ 6

Author(s):

Vasilios Panagopoulos ◽

Vasilios Liapis ◽

Irene Zinonos ◽

Shelley Hay ◽

Damien A. Leach ◽

...

Keyword(s):

Bone Resorption ◽

Osteoclast Differentiation ◽

Peroxidase Enzymes

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Biotransformation of Dibenzylbutanolides by Peroxidase Enzymes. Routes to the Podophyllotoxin Family.

Heterocycles ◽

10.3987/com-95-s65 ◽

1996 ◽

Vol 42 (2) ◽

pp. 479 ◽

Cited By ~ 15

Author(s):

James Peter Kutney ◽

Xinyao Du ◽

Rajina Naidu ◽

Nikolay M. Stoynov ◽

Masumi Takemoto

Keyword(s):

Peroxidase Enzymes

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Biochemical Profile of the Soybean Seed Embryonic Axis and Its Changes during Accelerated Aging

Biology ◽

10.3390/biology9080186 ◽

2020 ◽

Vol 9 (8) ◽

pp. 186

Author(s):

Luciano Antônio Ebone ◽

Andréia Caverzan ◽

Diógenes Cecchin Silveira ◽

Luciano de Oliveira Siqueira ◽

Nadia Canali Lângaro ◽

...

Keyword(s):

Ascorbate Peroxidase ◽

Cell Structure ◽

Soybean Seed ◽

Accelerated Aging ◽

Embryonic Axis ◽

Nutrient Analysis ◽

Maillard Reactions ◽

Seed Deterioration ◽

Randomized Experimental Design ◽

Peroxidase Enzymes

Seed deterioration is an important topic in plant science, as the majority of cultivated species use seeds as their means of propagation; however, due to its complexity, the process of seed deterioration has not yet been completely elucidated. Three soybean cultivars (BMX Raio, BMX Zeus, and DM 53i54) exposed to four distinct periods of accelerated aging (0, 3, 6 and 9 days) in a fully randomized experimental design. Initially, vigor and germination tests were performed. The activity of superoxide dismutase, catalase, ascorbate peroxidase enzymes, hydrogen peroxide, malonaldehyde, DNA oxidation, macromolecules and mineral content, and Maillard reactions were quantified in the embryonic axis. Results showed that DNA did not suffer degradation or oxidation. In terms of consumption of reserves, only sugars were consumed, while levels of protein, starch, and triglycerides were maintained. The Maillard reaction did show potential as an indicator of buffer capacity of protein to ROS. Additionally, levels of catalase and ascorbate peroxidase decreased during the aging process. Moreover, nutrient analysis showed that a high magnesium level in the cultivar bestowed greater resilience to deterioration, which can indicate a potential function of magnesium in the cell structure via reflex in seed aging through seed respiration.

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Radioprotective Role of Peroxiredoxin 6

Antioxidants ◽

10.3390/antiox8010015 ◽

2019 ◽

Vol 8 (1) ◽

pp. 15 ◽

Cited By ~ 15

Author(s):

Mars G. Sharapov ◽

Vladimir I. Novoselov ◽

Sergey V. Gudkov

Keyword(s):

Ionizing Radiation ◽

Mammalian Cells ◽

Redox Homeostasis ◽

Phospholipid Metabolism ◽

Pathological Effect ◽

Peroxiredoxin 6 ◽

Leading Role ◽

Wide Range ◽

Radiosensitive Organs ◽

Peroxidase Enzymes

Peroxiredoxin 6 (Prdx6) is a member of an evolutionary ancient family of peroxidase enzymes with diverse functions in the cell. Prdx6 is an important enzymatic antioxidant. It reduces a wide range of peroxide substrates in the cell, thus playing a leading role in the maintenance of the redox homeostasis in mammalian cells. Beside peroxidase activity, Prdx6 has been shown to possess an activity of phospholipase A2, an enzyme playing an important role in membrane phospholipid metabolism. Moreover, Prdx6 takes part in intercellular and intracellular signal transduction due to its peroxidase and phospholipase activity, thus facilitating the initiation of regenerative processes in the cell, suppression of apoptosis, and activation of cell proliferation. Being an effective and important antioxidant enzyme, Prdx6 plays an essential role in neutralizing oxidative stress caused by various factors, including action of ionizing radiation. Endogenous Prdx6 has been shown to possess a significant radioprotective potential in cellular and animal models. Moreover, intravenous infusion of recombinant Prdx6 to animals before irradiation at lethal or sublethal doses has shown its high radioprotective effect. Exogenous Prdx6 effectively alleviates the severeness of radiation lesions, providing normalization of the functional state of radiosensitive organs and tissues, and leads to a significant elevation of the survival rate of animals. Prdx6 can be considered as a potent and promising radioprotective agent for reducing the pathological effect of ionizing radiation on mammalian organisms. The radioprotective properties and mechanisms of radioprotective action of Prdx6 are discussed in the current review.

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ANATOMICAL DISTRIBUTION OF PEROXIDASE ISOZYMES IN BARLEY KERNELS

Canadian Journal of Plant Science ◽

10.4141/cjps75-105 ◽

1975 ◽

Vol 55 (3) ◽

pp. 661-666 ◽

Cited By ~ 6

Author(s):

D. E. LABERGE

Keyword(s):

Anatomical Location ◽

Barley Cultivar ◽

Hordeum Vulgare L ◽

Anatomical Distribution ◽

Peroxidase Isozyme ◽

Isozyme Patterns ◽

Peroxidase Isozymes ◽

Hydrogen Donors ◽

Green Malt ◽

Peroxidase Enzymes

Two barley (Hordeum vulgare L.) cultivars with different peroxidase isozyme patterns were studied using polyacrylamide-slab electrophoresis at pH 4.75 to separate the enzymes, and 3-amino-9-ethyl carbazole or o-dianisidine as hydrogen donors to detect peroxidase isozymes. Peroxidase isozyme patterns of extracts of very immature kernels up to 19 days post-anthesis were quite different from isozyme patterns from extracts of more mature kernels. During malting, the peroxidase isozymes of mature barely persisted in green malt, but an additional isozyme was detected in malt after 3 days of germination. Immature kernels with peroxidase isozyme patterns identical to those found in mature kernels for each barley cultivar were dissected into different tissue fractions including husks, pericarp, "green layer," aleurone, endosperm, embryo and scutellum. Electrophoresis of extracts of these tissues revealed the anatomical location of most of the peroxidase enzymes in the whole kernels of the two cultivars.

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Dynamic role of iron supply in amelioration of cadmium stress by modulating antioxidative pathways and peroxidase enzymes in mungbean

AoB Plants ◽

10.1093/aobpla/plz005 ◽

2019 ◽

Vol 11 (2) ◽

Cited By ~ 3

Author(s):

Krishnav Biyani ◽

Durgesh Kumar Tripathi ◽

Jeong Hyun Lee ◽

Sowbiya Muneer

Keyword(s):

Cadmium Stress ◽

Iron Supply ◽

Peroxidase Enzymes

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Conservation of Alstroemeria cut flowers stored under refrigeration

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v43i1.50016 ◽

2020 ◽

Vol 43 ◽

pp. e50016

Author(s):

Vanessa Cury Galati ◽

Ana Carolina Corrêa Muniz ◽

João Emmauel Ribeiro Guimarães ◽

Claudia Machado Fabrino Mattiuz ◽

Ben Hur Mattiuz

Keyword(s):

Storage Period ◽

Postharvest Quality ◽

Cut Flowers ◽

Floral Longevity ◽

Randomized Design ◽

Significant Difference ◽

Relative Water ◽

Peroxidase Enzymes ◽

Storage Temperatures

Alstroemeria flowers have shown great importance in the world trade of cut flowers due mainly to its beauty and wide variety of colors. However, the durability of its inflorescences is usually hampered by the rapid yellowing of the leaves, which impairs their decorative quality. Cut flowers require the use of technologies to improve postharvest quality and floral longevity. This research aimed to study the postharvest conservation of inflorescences of Alstroemeria cv. Ajax at different storage temperatures. Floral stems were placed in containers with distilled water and stored at four temperatures (4, 8, 12, and 22ºC) for 12 days. The following analyses were performed: fresh mass variation, respiratory activity, relative water content, soluble and reducing carbohydrate contents, polyphenol-oxidase and peroxidase enzymes, pigments (anthocyanin and carotenoids), and longevity. The experiment was conducted in a completely randomized design, the results were submitted to analysis of variance (ANOVA), and the effect of treatments submitted to F-test. Significant differences were compared using the least significant difference (LSD) at 95% confidence interval (p ≤ 0. 05). The temperatures of 8 and 12ºC were effective in maintaining the postharvest quality of inflorescences during storage period, as they remained turgid due to transpiration reduction caused by low temperatures, and longevity reaching 46 and 22 days, respectively.

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