REGULATION OF TRANSCRIPTION FACTOR SYNTHESIS

AbstractMalignant melanoma is highly resistant to conventional treatments and is one of the most aggressive types of skin cancers. Conventional cancer treatments are limited due to drug resistance, tumor selectivity, and solubility. Therefore, new treatments with fewer side effects and excellent effects should be developed. In previous studies, we have analyzed antimicrobial peptides (AMPs), which showed antibacterial and anti-inflammatory effects in insects, and some AMPs also exhibited anticancer efficacy. Anticancer peptides (ACPs) are known to have fewer side effects and high anticancer efficacy. In this study, the insect-derived peptide poecilocorisin-1 (PCC-1) did not induce toxicity in the human epithelial cell line HaCaT, but its potential as an anticancer agent was confirmed through specific effects of antiproliferation, apoptosis, and cell cycle arrest in two melanoma cell lines, SK-MEL-28 and G361. Additionally, we discovered a novel anticancer mechanism of insect-derived peptides in melanoma through the regulation of transcription factor Sp1 protein, which is overexpressed in cancer, apoptosis, and cell cycle-related proteins. Taken together, this study aims to clarify the anticancer efficacy and safety of insect-derived peptides and to present their potential as future therapeutic agents.

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Limits on information transduction through amplitude and frequency regulation of transcription factor activity

eLife ◽

10.7554/elife.06559 ◽

2015 ◽

Vol 4 ◽

Cited By ~ 60

Author(s):

Anders S Hansen ◽

Erin K O'Shea

Keyword(s):

Transcription Factor ◽

Target Genes ◽

Frequency Regulation ◽

Regulation Of Transcription ◽

Limited Information ◽

Dynamic Information ◽

Extrinsic Noise ◽

Intensity Information ◽

Single Target ◽

Activation Dynamics

Signaling pathways often transmit multiple signals through a single shared transcription factor (TF) and encode signal information by differentially regulating TF dynamics. However, signal information will be lost unless it can be reliably decoded by downstream genes. To understand the limits on dynamic information transduction, we apply information theory to quantify how much gene expression information the yeast TF Msn2 can transduce to target genes in the amplitude or frequency of its activation dynamics. We find that although the amount of information transmitted by Msn2 to single target genes is limited, information transduction can be increased by modulating promoter cis-elements or by integrating information from multiple genes. By correcting for extrinsic noise, we estimate an upper bound on information transduction. Overall, we find that information transduction through amplitude and frequency regulation of Msn2 is limited to error-free transduction of signal identity, but not signal intensity information.

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Dopaminergic regulation of transcription factor expression in organotypic cultures of developing striatum

Journal of Neuroscience ◽

10.1523/jneurosci.15-03-02367.1995 ◽

1995 ◽

Vol 15 (3) ◽

pp. 2367-2384 ◽

Cited By ~ 33

Author(s):

FC Liu ◽

H Takahashi ◽

RD McKay ◽

AM Graybiel

Keyword(s):

Transcription Factor ◽

Regulation Of Transcription ◽

Organotypic Cultures ◽

Factor Expression ◽

Dopaminergic Regulation ◽

Transcription Factor Expression

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Expression of miR159 Is Altered in Tomato Plants Undergoing Drought Stress

Plants ◽

10.3390/plants8070201 ◽

2019 ◽

Vol 8 (7) ◽

pp. 201 ◽

Cited By ~ 3

Author(s):

María José López-Galiano ◽

Inmaculada García-Robles ◽

Ana I. González-Hernández ◽

Gemma Camañes ◽

Begonya Vicedo ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Regulatory Networks ◽

Crop Yields ◽

Insect Pest ◽

Regulatory Function ◽

Myb Transcription Factor ◽

Regulation Of Transcription ◽

Tomato Plants

In a scenario of global climate change, water scarcity is a major threat for agriculture, severely limiting crop yields. Therefore, alternatives are urgently needed for improving plant adaptation to drought stress. Among them, gene expression reprogramming by microRNAs (miRNAs) might offer a biotechnologically sound strategy. Drought-responsive miRNAs have been reported in many plant species, and some of them are known to participate in complex regulatory networks via their regulation of transcription factors involved in water stress signaling. We explored the role of miR159 in the response of Solanum lycopersicum Mill. plants to drought stress by analyzing the expression of sly-miR159 and its target SlMYB transcription factor genes in tomato plants of cv. Ailsa Craig grown in deprived water conditions or in response to mechanical damage caused by the Colorado potato beetle, a devastating insect pest of Solanaceae plants. Results showed that sly-miR159 regulatory function in the tomato plants response to distinct stresses might be mediated by differential stress-specific MYB transcription factor targeting. sly-miR159 targeting of SlMYB33 transcription factor transcript correlated with accumulation of the osmoprotective compounds proline and putrescine, which promote drought tolerance. This highlights the potential role of sly-miR159 in tomato plants’ adaptation to water deficit conditions.

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The Sky's the LEMit: New insights into nuclear structure regulation of transcription factor activity

Current Opinion in Cell Biology ◽

10.1016/j.ceb.2020.10.006 ◽

2021 ◽

Vol 68 ◽

pp. 173-180

Author(s):

Amar N. Mirza ◽

Fernanda Gonzalez ◽

Sierra K. Ha ◽

Anthony E. Oro

Keyword(s):

Transcription Factor ◽

Nuclear Structure ◽

Regulation Of Transcription ◽

Transcription Factor Activity ◽

Factor Activity ◽

Structure Regulation

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On the Cooperation between Epigenetics and Transcription Factor Networks in the Specification of Tissue Stem Cells

Epigenomes ◽

10.3390/epigenomes2040020 ◽

2018 ◽

Vol 2 (4) ◽

pp. 20 ◽

Cited By ~ 6

Author(s):

Torsten Thalheim ◽

Lydia Hopp ◽

Hans Binder ◽

Gabriela Aust ◽

Joerg Galle

Keyword(s):

Stem Cells ◽

Transcription Factor ◽

Histone Methylation ◽

Epigenetic Modification ◽

Mechanistic Modeling ◽

Published Data ◽

Regulation Of Transcription ◽

Data Set ◽

Integrative View ◽

Transcription Factor Networks

It is generally accepted that epigenetic modifications, such as DNA and histone methylations, affect transcription and that a gene’s transcription feeds back on its epigenetic profile. Depending on the epigenetic modification, positive and negative feedback loops have been described. Here, we study whether such interrelation are mandatory and how transcription factor networks affect it. We apply self-organizing map machine learning to a published data set on the specification and differentiation of murine intestinal stem cells in order to provide an integrative view of gene transcription and DNA, as well as histone methylation during this process. We show that, although gain/loss of H3K4me3 at a gene promoter is generally considered to be associated with its increased/decreased transcriptional activity, such an interrelation is not mandatory, i.e., changes of the modification level do not necessarily affect transcription. Similar considerations hold for H3K27me3. In addition, even strong changes in the transcription of a gene do not necessarily affect its H3K4me3 and H3K27me3 modification profile. We provide a mechanistic explanation of these phenomena that is based on a model of epigenetic regulation of transcription. Thereby, the analyzed data suggest a broad variance in gene specific regulation of histone methylation and support the assumption of an independent regulation of transcription by histone methylation and transcription factor networks. The results provide insights into basic principles of the specification of tissue stem cells and highlight open questions about a mechanistic modeling of this process.

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