Multiplex event-specific qualitative polymerase chain reaction for detecting three transgenic rice lines and application of a standard plasmid as a quantitative reference molecule

This article describes the contemporary methods of diagnosing sexually transmitted infections, their advantages and disadvantages, indications for use. The authors describe application of quantitative polymerase chain reaction in diagnosing inflammatory diseases and dysbiotic conditions in men and women. This method, which is currently the “golden standard” in urogenital pathology diagnostics, has undeniable advantages over microbiological methods and qualitative polymerase chain reaction: the preanalytical stage requirements (preservation of quantitative ratios between microorganisms or nucleic acids of microorganisms) are not as strict, the risk of contamination from outside environment and subsequent corruption of the results is significantly smaller, the conditions for all microorganisms, including those impossible and hard to cultivate, are the same sensitivity and specificity-wise, it is possible to sample materials and evaluate microbiota (ratios of microorganisms and their groups) and also possible to collect samples non-invasively, the speed of testing is high.

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Duplex Polymerase Chain Reaction (PCR) for the Simultaneous Detection ofCryia(B)and the Maize Ubiquitin Promoter in the Transgenic Rice Line Kmd1

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2008.10817538 ◽

2008 ◽

Vol 22 (2) ◽

pp. 705-708 ◽

Cited By ~ 5

Author(s):

R. Babekova ◽

T. Funk ◽

S. Pecoraro ◽

K.-H. Engel ◽

D. Baikova ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Transgenic Rice ◽

Simultaneous Detection ◽

Ubiquitin Promoter ◽

Chain Reaction ◽

Rice Line ◽

Transgenic Rice Line ◽

Maize Ubiquitin Promoter ◽

Polymerase Chain ◽

Duplex Polymerase Chain Reaction

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Event-specific quantitative polymerase chain reaction methods for detection of double-herbicide-resistant genetically modified corn MON 87419 based on the 3′-junction of the insertion site

Bioscience Biotechnology and Biochemistry ◽

10.1093/bbb/zbab040 ◽

2021 ◽

Author(s):

Likun Long ◽

Wei Yan ◽

Congcong Li ◽

Liming Dong ◽

Na Liu ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Genetically Modified ◽

Quantitative Polymerase Chain Reaction ◽

Insertion Site ◽

Chain Reaction ◽

Herbicide Resistant ◽

Qualitative Polymerase Chain Reaction ◽

Relative Standard ◽

Junction Site ◽

Polymerase Chain

ABSTRACT MON 87419 was one of the new transgenic corn events developed in US with the trait of herbicide resistance to both dicamba and glyphosate. To monitor unintended release of genetically modified organism in the future, as well as to meet GM-labeling requirements, it is requisite to develop a reliable method for the detection and quantification of MON 87419, an event-specific primer pair was designed to amplify the 3′-junction site between the endogenous genome sequence and the transferred DNA of GM event MON 87419, amplicons of desired size were produced by qualitative polymerase chain reaction (PCR) assay. For the validation of this quantitative method, the mixed samples containing 10%, 1%, and 0.1% MON 87419 ingredient were quantified. The precisions were expressed as relative standard deviations, deviated by 7.87%, 12.94%, and 19.98%, respectively. These results clearly demonstrate that the PCR methods we developed herein can be used for event-specific quantitative testing of the double-herbicide-resistant corn MON 87419.

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