Molecular detection, identification and phylogenetic inference of Leishmania spp. in some desert lizards from Northwest China by using internal transcribed spacer 1 (ITS1) sequences

Acta Tropica ◽  
2016 ◽  
Vol 162 ◽  
pp. 83-94 ◽  
Author(s):  
Jun-Rong Zhang ◽  
Xian-Guang Guo ◽  
Jin-Long Liu ◽  
Tian-He Zhou ◽  
Xiong Gong ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Molecular Detection ◽  
Phylogenetic Inference ◽  
Northwest China ◽  
Leishmania Spp

scholarly journals Multi-locus characterization and phylogenetic inference of Leishmania spp. in snakes from Northwest China

2019 ◽  
Author(s):  
Han Chen ◽  
Jiao Li ◽  
Junrong Zhang ◽  
Jinlei He ◽  
Jianhui Zhang ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Natural Infection ◽  
Protozoan Parasite ◽  
Phylogenetic Inference ◽  
Northwest China ◽  
Future Research ◽  
Network Analyses ◽  
Epidemic Area ◽  
Potential Reservoir ◽  
Leishmania Spp

AbstractBackgroundLeishmaniasis caused by protozoan parasite Leishmania is a neglected disease which is endemic in the northwest of China. Reptiles were considered to be the potential reservoir hosts for mammalian Leishmaniasis, and Leishmania had been detected in lizards from the epidemic area in the northwest of China. To date, few studies are focused on the natural infection of snakes with Leishmania.MethodsIn this study, 15 snakes captured from 10 endemic foci in the northwest of China were detected Leishmania spp. on the base of mitochondrial cytochrome b, heat shock protein 70 gene and ribosomal internal transcribed spacer 1 regions, and identified with phylogenetic and network analyses.ResultIn total, Leishmania gene was found in 7 snakes. The phylogenetic inference trees and network analysis suggests that the species identification was confirmed as Leishmania donovani, Leishmania turanica and Leishmania sp.ConclusionOur work is the first time to investigate the natural Leishmania spp. infection of snakes in the northwest of China. Mammalian Leishmania was discovered in snakes and the reptilian Leishmania was closely related to the clinical strains both prompt the importance of snakes in the disease cycle. To indicate the epidemiological involvement of snakes, a wide sample size in epidemic area and the pathogenic features of reptilian Leishmania promastigotes are recommended in the future research.

scholarly journals Multi-locus characterization and phylogenetic inference of Leishmania spp. in snakes from Northwest China

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0210681 ◽  
Author(s):  
Han Chen ◽  
Jiao Li ◽  
Junrong Zhang ◽  
Xianguang Guo ◽  
Jinlong Liu ◽  
...  
Keyword(s):  
Phylogenetic Inference ◽  
Northwest China ◽  
Leishmania Spp

scholarly journals Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1

2019 ◽  
Vol 28 (2) ◽  
pp. 303-305
Author(s):  
Geovanna Vioti ◽  
João Augusto Franco Leonel ◽  
Kleber Menegon Lemes ◽  
Vanessa Figueredo Pereira ◽  
Helena Lage Ferreira ◽  
...  
Keyword(s):  
Public Health ◽  
Visceral Leishmaniasis ◽  
Internal Transcribed Spacer ◽  
Leishmania Infantum ◽  
Endemic Area ◽  
Molecular Detection ◽  
The State ◽  
Base Pairs ◽  
Blood Samples ◽  
Leishmania Spp

Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.

Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences

2010 ◽  
Vol 107 (5) ◽  
pp. 1049-1065 ◽  
Author(s):  
Bin-Bin Yang ◽  
Xian-Guang Guo ◽  
Xiao-Su Hu ◽  
Jian-Guo Zhang ◽  
Lin Liao ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Inference ◽  
Species Discrimination

scholarly journals Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

2013 ◽  
Vol 22 (4) ◽  
pp. 605-607 ◽  
Author(s):  
Willian Marinho Dourado Coelho ◽  
Katia Denise Saraiva Bresciani
Keyword(s):  
Visceral Leishmaniasis ◽  
Deoxyribonucleic Acid ◽  
Molecular Detection ◽  
Lutzomyia Longipalpis ◽  
Sand Flies ◽  
Thoracic Region ◽  
Main Species ◽  
Leishmania Spp ◽  
Genus Leishmania ◽  
Horse Fly

Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmaniaspp. in dipterous insects of the species T. importunus.

scholarly journals Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia

2018 ◽  
Vol 30 (6) ◽  
pp. 942-945 ◽  
Author(s):  
Abdullah D. Alanazi ◽  
Robert Puschendorf ◽  
Bashir Salim ◽  
Mohamed S. Alyousif ◽  
Ibrahim O. Alanazi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Internal Transcribed Spacer ◽  
Molecular Detection ◽  
Cross Sectional Study ◽  
Trypanosoma Evansi ◽  
Type B ◽  
Sectional Study ◽  
Internal Transcribed Spacer Region ◽  
Cross Sectional ◽  
Species Specific

We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species–specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.

Evaluation of a point-of-care molecular detection device for Leishmania spp. and intercurrent fungal and mycobacterial organisms in Peruvian patients with cutaneous ulcers

Infection ◽  
2021 ◽  
Author(s):  
Ruwandi Kariyawasam ◽  
Braulio M. Valencia ◽  
Rachel Lau ◽  
Eric Shao ◽  
Courtney A. Thompson ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Point Of Care ◽  
Leishmania Spp

scholarly journals Pathogenic Leishmania spp. detected in lizards from Northwest China using molecular methods

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Jun-Rong Zhang ◽  
Xian-Guang Guo ◽  
Han Chen ◽  
Jin-Long Liu ◽  
Xiong Gong ◽  
...  
Keyword(s):  
Heat Shock Protein 70 ◽  
Detection Rate ◽  
New World ◽  
Northwest China ◽  
Molecular Methods ◽  
Mixed Infections ◽  
Chain Reaction ◽  
Blood Samples ◽  
Leishmania Spp ◽  
Polymerase Chain

Abstract Background Leishmaniosis, a disease caused by pathogenic Leishmania parasites, remains an unresolved health problem in the New World and the Old World. It is well known that lizards can be infected by a subgenus of Leishmania parasites, i.e. Sauroleishmania, which is non-pathogenic to humans. However, evidence suggests that lizards may also harbor pathogenic Leishmania species including the undetermined Leishmania sp., discovered in our previous work. Leishmania DNA in lizard blood can be detected by using molecular methods, such as the polymerase chain reaction (PCR). Results Three hundred and sixteen lizards, representing 13 species of four genera, were captured for blood samples collection in Northwest China. Two reliable molecular markers (cytochrome b and heat shock protein 70 genes) were used for detection in the lizard blood samples, to confirm a widespread presence of pathogenic Leishmania parasites and the distribution pattern of Leishmania spp. in lizards from Northwest China. The PCR data indicated positive detection rate for Leishmania in all the tested lizards with an overall prevalence of 57.91% (183/316). Apart from lizard parasites like Leishmania tarentolae and Leishmania sp., several pathogenic Leishmania including L. turanica, L. tropica and L. donovani complex were identified by using phylogenetic analysis. Co-existence of different haplotypes was observed in most Leishmania DNA-positive lizards with an overall rate of 77.6% (142/183). Even mixed infections with different Leishmania species appeared to occur in the lizards with an overall rate of 37.7% (69/183). Conclusions Lizards can harbor pathogenic Leishmania spp. Co-existence of different haplotypes or even species of Leishmania indicates mixed infections in natural lizard host. Lizards may contribute to the spread of Leishmania parasites. The pathogenic Leishmania species detected in lizards from Northwest China may be of great eco-epidemiological importance.

scholarly journals Molecular detection of Leishmania spp. in dogs and a cat from Doha, Qatar

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Clara Lima ◽  
Vito Colella ◽  
Maria Stefania Latrofa ◽  
Luís Cardoso ◽  
Domenico Otranto ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Leishmania Spp
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