scholarly journals Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias

2021 ◽  
Vol 66 (2) ◽  
pp. 411-417
Author(s):  
Ewa Moric-Janiszewska ◽  
Sławomir Smolik ◽  
Aleksandra Morka ◽  
Lesław Szydłowski ◽  
Małgorzata Kapral
Keyword(s):  
Pediatric Patients ◽  
Supraventricular Arrhythmias ◽  
Circulating Micrornas ◽  
Expression Levels

scholarly journals Expression Levels of Candidate Circulating microRNAs in Early-Onset Neonatal Sepsis Compared With Healthy Newborns

2018 ◽  
Vol 11 ◽  
pp. 117863101879707 ◽  
Author(s):  
Benet B Dhas ◽  
Vijaya R Dirisala ◽  
B Vishnu Bhat
Keyword(s):  
Neonatal Sepsis ◽  
Inflammatory Responses ◽  
Diagnostic Techniques ◽  
Diagnostic Methods ◽  
Large Sample Size ◽  
Case Control Studies ◽  
Circulating Micrornas ◽  
Expression Levels ◽  
Sepsis Diagnosis ◽  
Low Sensitivity

The high mortality rate of neonatal sepsis is directly connected with time-consuming diagnostic methods that have low sensitivity and specificity. The need of the hour is to develop novel diagnostic techniques that are rapid and more specific. In this study, we estimated the expression levels of circulating microRNAs (miRNAs) that are involved in regulating immune response genes and underlying inflammatory responses, which may be used for sepsis diagnosis. The total circulating miRNA was isolated and the candidate miRNAs (miR-132, miR-146a, miR-155, and miR-223) were quantified by real-time polymerase chain reaction technique. Statistical analysis revealed that miR-132 ( P < .01) and miR-223 ( P < .05) were downregulated in septic newborns compared with healthy babies. The decrease in expression of miR-132 and miR-223 may be associated with increased expression of immune-related genes involved in TLR (Toll-like receptor) signaling pathway. Further case-control studies with large sample size are required to identify the potential of miRNAs in neonatal sepsis diagnosis.

Circulating microRNAs overexpressed in macrosomia: an experimental and bioinformatic approach

2020 ◽  
Vol 11 (5) ◽  
pp. 464-472
Author(s):  
Alejandra Ortiz-Dosal ◽  
Elvira del Carmen Arellanes-Licea ◽  
Patricia Rodil-García ◽  
Luis A. Salazar-Olivo
Keyword(s):  
Birth Weight ◽  
Target Genes ◽  
Metabolic Diseases ◽  
Dried Blood Spots ◽  
Predictive Tool ◽  
Circulating Micrornas ◽  
Metabolic Alterations ◽  
Expression Levels ◽  
Blood Spots ◽  
Obesity And Diabetes

AbstractLow birth weight (LBW) and macrosomia have been associated with later-in-life metabolic alterations. The aim of this study was to elucidate whether the expression levels of circulating microRNAs (c-miRNAs) associated with adult metabolic diseases are also dysregulated in newborns with LBW or macrosomia. The expression levels of five microRNAs (miRNAs) associated with metabolic diseases were quantified in dried blood spots of newborns with adequate birth weight, LBW and macrosomia by stem-loop real-time polymerase chain reaction. miR-29a-5p, miR-126-3p, miR-221-3p, and miR-486-5p were significantly overexpressed in newborns with macrosomia and showed no significant change in the LBW group compared to normal weight controls. miR-320a showed no statistical difference among groups. We predicted the putative target genes and pathways of the overexpressed miRNAs with bioinformatic tools. Bioinformatic analyses of overexpressed miRNAs predicted target genes involved in carbohydrate metabolism, participate in FoxO and PI3K/Akt signaling pathways, and are associated with diabetes, obesity, and cardiovascular diseases. The overexpression of circulating miR-29a-5p, miR-126-3p, miR-221-3p, and miR-486-5p may explain the increased risk of obesity and diabetes associated with macrosomia. The use of dried blood spots from newborn screening cards to quantify miRNAs expression levels could be an early and minimally invasive predictive tool for these metabolic alterations.

scholarly journals MicroRNA Era: The Importance for Diagnosis and Prognosis of Adrenocortical Tumors

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
João Evangelista Bezerra ◽  
Ana Claudia Latronico
Keyword(s):  
Gene Expression ◽  
Posttranscriptional Regulation ◽  
Essential Role ◽  
Pediatric Patients ◽  
Regulation Of Gene Expression ◽  
Adrenal Tumors ◽  
Expression Levels ◽  
Adrenocortical Tumors ◽  
Diagnosis And Prognosis ◽  
Current Review

MicroRNAs play an essential role in posttranscriptional regulation of gene expression. They are evolutionary conserved, small, noncoding, 19–22-nucleotide RNAs, whose abnormalities, such as up- or downregulated expression, have been associated with several neoplasms, including adrenocortical tumors. Expression levels of distinct microRNAs can distinguish benign from malignant adrenal tumors. This current review provides recent data on the miRNAs profile in benign and malignant adrenocortical tumors diagnosed in adult and pediatric patients.

scholarly journals Genotypic Characteristics of Hepatoblastoma as Detected by Next Generation Sequencing and Their Correlation With Clinical Efficacy

2021 ◽  
Vol 11 ◽  
Author(s):  
Huimin Hu ◽  
Weiling Zhang ◽  
Tian Zhi ◽  
Jing Li ◽  
Yuan Wen ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Survival Rate ◽  
Mismatch Repair ◽  
Pediatric Patients ◽  
Next Generation ◽  
Expression Levels ◽  
Dna Mismatch ◽  
Mismatch Repair Status ◽  
Generation Sequencing ◽  
Potential Biomarkers

BackgroundHepatoblastoma (HB) is the most common malignant embryonic liver tumor type in children under 3 years of age. In the present study, the next generation sequencing (NGS) method was used to detect the genotype characteristics of HB and summarize the correlation between the common mutation genotypes noted in this disease and the clinical treatment and prognosis. The results may aid clinical prognosis and the successful application of targeted drugs.MethodsInitially, DNA was extracted from tumor tissue specimens and peripheral blood derived from 19 pediatric patients with HB. Subsequently, DNA panel and NGS methods were used to detect tumor diagnosis and the expression levels of treatment-associated genes, followed by the summary of genotype characteristics. In addition, in order to further assess the application of immunotherapy in HB, immunohistochemical detection of programmed cell death 1 ligand 1 (PDL1) was performed in combination with tumor mutation burden (TMB) and DNA mismatch repair status analysis. Furthermore, the clinical treatment effect and prognosis of the pediatric patients were statistically analyzed according to the characteristics of the genotype. Overall prognosis and prognostic analyses in different groups were performed by Kaplan-Meier and log-rank tests, respectively. Finally, expression validation and diagnostic analysis of commonly reported genes were performed in the GSE75271 dataset, which was obtained from the Gene Expression Omnibus (GEO) database.ResultsIn the present study, certain mutated genes, including nuclear factor erythroid 2-related factor 2 (NFE2L2), catenin β1 (CTNNB1), MYCN, tumor protein p53, axis inhibition protein 1 (AXIN1) and adenomatous polyposis coli (APC) were associated with the pathogenesis of HB. During TMB and DNA mismatch repair status analyses, pediatric patients had a low TMB. All of them did not present with microsatellite instability. The immunohistochemical results indicated lower expression levels of PDL1 in HB. The complete remission (CR) rate of pediatric patients in the gene abnormality group was lower than that of the non-reported disease-associated gene abnormality group. The 2-year overall survival rate and disease-free survival rate of 19 pediatric patients with HB were 72.1% and 42.4%, respectively. Receiver operating characteristic (ROC) analysis demonstrated that CTNNB1, NFE2L2, AXIN1, APC, MYCN and insulin growth factor 2 (IGF2) may be potential biomarkers that could be used for the diagnosis of HB.ConclusionThe genotype changes in HB were more common and the CR rate of the pediatric patients with an altered genotype was lower than that of pediatric patients without an altered genotype. In addition, pediatric patients with HB exhibited lower TMB compared with adult patients. Moreover, the data indicated that CTNNB1, NFE2L2, AXIN1, APC, MYCN and IGF2 may be potential biomarkers that can be used for the diagnosis of HB.

Downregulation of Circulating miR-23a-3p, miR-101-3p And miR-let-7c In Women With Idiopathic Recurrent Pregnancy Loss

2021 ◽  
Author(s):  
Mina Tutunfroush ◽  
Saeid Ghorbian ◽  
Jafar Mohseni ◽  
Shahla Danaii
Keyword(s):  
Healthy Subjects ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Diagnostic Value ◽  
Clinicopathological Parameters ◽  
Circulating Micrornas ◽  
Expression Levels ◽  
Non Invasive ◽  
Significant Difference ◽  
History Of

Abstract Recently, circulating microRNAs have attracted much attention because they can serve as reliable non-invasive diagnostic and prognostic biomarkers for pregnancy-related complications. So, this study aimed to quantify miR-23a-3p, miR-101-3p and miR-let-7c expression levels in plasma of patients with idiopathic recurrent pregnancy loss (iRPL) and healthy subjects and to evaluate their potential diagnostic value in iRPL patients. A total of 120 plasma samples were obtained from sixty women with a history of at least two consecutive iRPL and sixty healthy women without a history of miscarriage to evaluate the expression levels of the circulating miR-23a-3p, miR-101-3p and miR-let-7c by quantitative real-time polymerase chain reaction (qPCR) technique. The correlation between studied miRNAs and clinicopathological parameters was also assessed. Receiver operating characteristic (ROC) curve was plotted to determine the diagnostic accuracy of miR-23a-3p, miR-101-3p and miR-let-7c in iRPL. Our results showed that the miR-23a-3p expression level in plasma of iRPL patients was lower than those in healthy controls but without a statistically significant difference (P = 0.113). The expression levels of miR-101-3p and miR-let-7c were significantly downregulated in iRPL patients compared with healthy subjects (P < 0.05). The expression levels of miR-23a-3p and miR-let-7c was negatively correlated with number of abortions in iRPL patients. We observed statistically significant positive correlation between miR-23a-3p and miR-101-3p (r = 0.478, P = 0.001), miR-23a-3p and miR-let-7c (r = 0.561, P = 0.0001), miR-101-3p and miR-let-7c (r = 0.533, P = 0.0001) in patients with iRPL. The current study provides evidence indicating that downregulation of miR-23a-3p, miR-101-3p and miR-let-7c may be associated with iRPL.

CLINICAL IMPORTANCE OF CIRCULATING AND CELLULAR EXPRESSION LEVELS OF FAS AND FAS LIGAND IN PEDIATRIC PATIENTS WITH LYMPHOPROLIFERATIVE MALIGNANCIES

2005 ◽  
Vol 22 (3) ◽  
pp. 247-256 ◽  
Author(s):  
Volkan Hazar ◽  
Zafer Berber ◽  
Elif Pestereli ◽  
Mesut Coskun ◽  
Akif Yesilipek ◽  
...  
Keyword(s):  
Fas Ligand ◽  
Pediatric Patients ◽  
Clinical Importance ◽  
Expression Levels ◽  
Cellular Expression

Expression levels of circulating microRNAs-126, -155, and -145 in Mexican women exposed to polycyclic aromatic hydrocarbons through biomass fuel use

2019 ◽  
Vol 60 (6) ◽  
pp. 546-558 ◽  
Author(s):  
Tania Ruiz-Vera ◽  
Ángeles C. Ochoa-Martínez ◽  
Lucía G. Pruneda-Álvarez ◽  
Gabriela Domínguez-Cortinas ◽  
Ivan N. Pérez-Maldonado
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Biomass Fuel ◽  
Mexican Women ◽  
Fuel Use ◽  
Circulating Micrornas ◽  
Expression Levels ◽  
Polycyclic Aromatic

scholarly journals Circulating microRNAs associated with prediabetes and geographic location in Latinos

2021 ◽  
Author(s):  
Elena Flowers ◽  
Juan-Daniel Ramírez-Mares ◽  
Marion Velazquez-Villafaña ◽  
Ruben Rangel-Salazar ◽  
Anatol Sucher ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Fasting Blood Glucose ◽  
The United States ◽  
Lifestyle Factors ◽  
Glycemic Status ◽  
Cross Sectional ◽  
Circulating Micrornas ◽  
Expression Levels ◽  
The Us

Abstract Background Globally, type 2 diabetes is highly prevalent in individuals of Latino ancestry. The reasons underlying this high prevalence are not well understood, but both genetic and lifestyle factors are contributors. Circulating microRNAs are readily detectable in blood and are promising biomarkers to characterize biological responses (i.e., changes in gene expression) to lifestyle factors. Prior studies identified relationships between circulating microRNAs and risk for type 2 diabetes, but Latinos have largely been under-represented in these study samples. Aims/hypothesis The aim of this study was to assess for differences in expression levels of three candidate microRNAs (miR-126, miR-146, miR-15) between individuals who had prediabetes compared to normal glycemic status and between individuals who self-identified with Latino ancestry in the United States (US) and native Mexicans living in or near Leon, Mexico. Methods This was a cross-sectional study that included 45 Mexicans and 21 Latino participants from the US. Prediabetes was defined as fasting glucose 100–125 mg/dL or 2-h post-glucose challenge between 140 and 199 mg/dL. Expression levels of microRNAs from plasma were measured by qPCR. Linear and logistic regression models were used to assess relationships between individual microRNAs and glycemic status or geographic site. Results None of the three microRNAs was associated with risk for type 2 diabetes. MiR-146a and miR-15 were significantly lower in the study sample from Mexico compared to the US. There was a significant interaction between miR-146a and BMI associated with fasting blood glucose. Conclusions/interpretation This study did not replicate in Latinos prior observations from other racial groups of associations between miR-126, miR-146a, and miR-15 and risk for type 2 diabetes. Future studies should consider other microRNAs related to different biological pathways as possible biomarkers for type 2 diabetes in Latinos.

scholarly journals THE MUCOSAL MICRORNA PROFILE RELATES TO AGE AND SEVERITY OF DISEASE IN PATIENTS WITH ULCERATIVE COLITIS

2021 ◽  
Vol 27 (Supplement_1) ◽  
pp. S11-S11
Author(s):  
Mikkel Malham ◽  
Jaslin James ◽  
Christian Jakobsen ◽  
Estrid Høgdall ◽  
Kim Holmstroem ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Disease Severity ◽  
Mirna Expression ◽  
Pediatric Patients ◽  
Severe Disease ◽  
Adult Patients ◽  
Expression Levels ◽  
Microrna Profile ◽  
Severity Of The Disease

Abstract Despite significant evidence that the expression of several microRNA’s (miRNA) impacts on disease activity in patients with ulcerative colitis (UC), it remains unknown if the more severe disease phenotype seen in pediatric-onset UC can be explained by altered miRNA expression. In this study, we aimed to assess the relationship between miRNA expression, age, and disease severity in pediatric and adult patients with UC. Using RT-qPCR, we analyzed the expression of miR-21, miR-31, miR-126, miR-142, and miR-155 in paraffin-embedded rectum biopsies from 30 pediatric and 30 adult-onset UC patients, and found that lesions from adult patients had significantly higher expression levels of miR-21 compared to pediatric patients and that the expression levels of miR-31 (all patients) and miR-155 (pediatric patients only) correlated inversely with histological assessed disease severity. Using in situ hybridization followed by image analysis, the expression estimates of miR-21 and miR-126 were found to correlate with histological assessed disease severity. In conclusion, we found that the expression of miRNAs depends on the age of the patient and/or the severity of the disease, suggesting that miRNAs may contribute to the regulation of inflammation in UC and could be useful biomarkers in the surveillance of disease severity.

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