Effects of non-esterified fatty acids on relative abundance of prostaglandin E2 and F2α synthesis-related mRNA transcripts and protein in endometrial cells of cattle in vitro

2020 ◽  
Vol 221 ◽  
pp. 106549
Author(s):  
Xueqiang Qin ◽  
Shuhua Yang ◽  
Yi Zhang ◽  
Lin Li ◽  
Peng Li ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Prostaglandin E2 ◽  
Relative Abundance ◽  
Endometrial Cells ◽  
Esterified Fatty Acids ◽  
Mrna Transcripts

Inhibition of binding of gonadal steroids to serum binding proteins by non-esterified fatty acids: the influence of chain length and degree of unsaturation

1989 ◽  
Vol 120 (2) ◽  
pp. 175-179 ◽  
Author(s):  
C. Street ◽  
R. J. S. Howell ◽  
L. Perry ◽  
S. Al-Othman ◽  
T. Chard
Keyword(s):  
Fatty Acids ◽  
Protein Binding ◽  
Unsaturated Fatty Acids ◽  
Late Pregnancy ◽  
Sex Hormone Binding Globulin ◽  
Partial Purification ◽  
Normal Female ◽  
Esterified Fatty Acids ◽  
Vitro Binding

Abstract. The effect of non-esterified fatty acids (NEFA) on the in vitro binding of testosterone, 5-alpha dihydrotestosterone and estradiol E2 to sex hormone binding globulin (SHBG) was examined using pooled normal female serum, and SHBG and albumin fractions obtained from the partial purification of late pregnancy serum. A range of saturated and unsaturated fatty acids were examined for their effect on steroid-protein binding. In normal female serum, NEFA added at physiological concentrations disrupted steroid-protein binding. The shorter chain (C8–C12) saturated acids and the poly-unsaturated acids proved to be more effective inhibitors than the longer chain saturated or mono-unsaturated acids. The greatest inhibition was obtained with E2 whereas the binding of dihydrotestosterone was least affected. With partially purified SHBG, the same concentrations of NEFA were less effective at inhibiting the binding of dihydrotestosterone and testosterone but elicited the same effect with E2. The binding of steroids to albumin appeared to be unaffected by these concentrations of NEFA.

scholarly journals Cocultured porcine granulosa cells respond to excess non-esterified fatty acids during in vitro maturation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Meihong Shi ◽  
Marc-André Sirard
Keyword(s):  
Fatty Acids ◽  
Granulosa Cells ◽  
Epithelial Mesenchymal Transition ◽  
Differentially Expressed ◽  
Algorithm Analysis ◽  
Inflammatory Factors ◽  
Luteal Cell ◽  
Mesenchymal Transition ◽  
Esterified Fatty Acids

Abstract Background Non-esterified fatty acids (NEFAs) are one of the main lipid components of follicular fluid at concentrations that depend on circulating levels. Elevated levels of NEFAs impair oocyte quality, development potential, and may subsequently influence the metabolism and reproductive fitness of offspring. Granulosa cells (GCs) are the follicular cells that are closely communicating with the oocyte. However, the responses of GCs exposed to high levels of NEFAs when cocultured with cumulus-oocyte complexes (COCs), and how they attenuate the negative effects of NEFAs on oocytes, are unclear. Results To better understand this protective effect, monolayers of porcine GCs were cocultured with COCs during in vitro maturation (IVM) in the presence of elevated levels of NEFAs. Genomic expression analysis was conducted to explore the responses of the GCs to the elevated levels of NEFAs. After limma algorithm analysis, 1,013 genes were differentially expressed between GCs cultured with and without elevated NEFAs. Among them, 438 genes were upregulated and 575 were downregulated. The differentially expressed genes were enriched in pathways related to metabolism, inflammation, and epithelial-mesenchymal transition. Conclusions The pathways and upstream regulators suggested that the cocultured GCs responded to the elevated NEFAs with (1) inhibition of the transition from granulosa to luteal cell, (2) interactions of metabolism change, anti-inflammation, mitochondrial function, and cell transition, (3) intercommunication with cocultured COCs of anti-inflammatory factors.

THE EFFECT OF VARIOUS SACCHARIDES ON THE RELEASE OF NON ESTERIFIED FATTY ACIDS FROM ADIPOSE TISSUE IN VITRO

1962 ◽  
Vol 40 (4) ◽  
pp. 455-458 ◽  
Author(s):  
W. F. Perry ◽  
R. J. Tjaden
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Incubation Medium ◽  
Epididymal Adipose Tissue ◽  
Tissue Cell ◽  
Glycerol Phosphate ◽  
Esterified Fatty Acids ◽  
Adipose Tissue Cell ◽  
Other Substances

Rat epididymal adipose tissue was incubated in a phosphate–albumin medium to ascertain the effect of various saccharides and other substances on the release of non-esterified fatty acids (NEFA) into the medium. It was found that incubation with glucose, mannose, fructose, and 2-deoxy glucose resulted in less release of NEFA from the tissue into the incubation medium. Incubation with galactose, sucrose, lactose, D-ribose, D-xylose, L-xylose, D-arabinose, L-arabinose, D-lyxose, sodium pyruvate, glycerol, and glycerol phosphate showed no differences from the control in release of NEFA into the incubation medium. These results are consistent with the theory that the NEFA-lowering action of glucose is due to esterification of free fatty acid within the adipose tissue cell by glycerol phosphate.

The effects of non-esterified fatty acids and β-hydroxybutyrate on the hepatic CYP2E1 in cows with clinical ketosis

2019 ◽  
Vol 86 (1) ◽  
pp. 68-72
Author(s):  
Zhicheng Peng ◽  
Xiaobing Li ◽  
Zhe Wang ◽  
Guowen Liu ◽  
Xinwei Li
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Dairy Cows ◽  
Blood Concentrations ◽  
Cytochrome P4502e1 ◽  
Esterified Fatty Acids ◽  
Cyp2e1 Expression ◽  
Expression And Activity

AbstractDairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis exhibited a low blood concentration of glucose but high concentrations of NEFA and BHB. Hepatic mRNA, protein expression, and activity of CYP2E1 were significantly higher in cows with clinical ketosis than in control cows. In vitro, both NEFA and BHB treatment markedly up-regulated the mRNA and protein expressions as well as activity of CYP2E1 in cow hepatocytes. Taken together, these results indicate that high levels of NEFA and BHB significantly up-regulate the expression and activity of hepatic CYP2E1, and may be influential in the induction of oxidative stress in cows with clinical ketosis.

scholarly journals Metabolism of endogenous sterol ester by the superovulated rat ovary in vitro

1970 ◽  
Vol 116 (1) ◽  
pp. 79-82 ◽  
Author(s):  
A. P. F. Flint ◽  
R. M. Denton
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Sterol ◽  
Sterol Ester ◽  
Esterified Fatty Acids ◽  
Mammalian Tissues ◽  
Endogenous Substrate ◽  
Rat Ovary

Sterol, glyceride and phospholipid were found to account for more than 90% (w/w) of the lipid extracted from whole superovulated rat ovaries. These lipids, together with non-esterified fatty acids, were assayed in slices of the tissue after incubation for various times. Whereas the concentrations of triglyceride, diglyceride and phospholipid did not change significantly during incubation, that of sterol ester markedly decreased and those of free sterol, monoglyceride and non-esterified fatty acid increased. Evidence is presented that in this tissue (in contrast with other mammalian tissues) the main endogenous substrate for respiration is fatty acid derived from sterol ester.

Effect of non-esterified fatty acids on bovine theca cell steroidogenesis and proliferation in vitro

2006 ◽  
Vol 92 (1-2) ◽  
pp. 51-63 ◽  
Author(s):  
T. Vanholder ◽  
J. Lmr Leroy ◽  
A. Van Soom ◽  
D. Maes ◽  
M. Coryn ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Theca Cell ◽  
Esterified Fatty Acids ◽  
Proliferation In Vitro

Non-esterified fatty acids activate the ROS–p38–p53/Nrf2 signaling pathway to induce bovine hepatocyte apoptosis in vitro

APOPTOSIS ◽  
2014 ◽  
Vol 19 (6) ◽  
pp. 984-997 ◽  
Author(s):  
Yuxiang Song ◽  
Xinwei Li ◽  
Yu Li ◽  
Na Li ◽  
Xiaoxia Shi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Signaling Pathway ◽  
Hepatocyte Apoptosis ◽  
Esterified Fatty Acids ◽  
Nrf2 Signaling Pathway

Non-esterified fatty acids impair endothelium-dependent vasodilation in rat mesenteric resistance vessels

2004 ◽  
Vol 107 (6) ◽  
pp. 625-629 ◽  
Author(s):  
Christopher A. R. SAINSBURY ◽  
Naveed SATTAR ◽  
John M. C. CONNELL ◽  
Chris HILLIER ◽  
John R. PETRIE
Keyword(s):  
Fatty Acids ◽  
Ascorbic Acid ◽  
Endothelial Dysfunction ◽  
Palmitic Acid ◽  
Resistance Arteries ◽  
Response Curves ◽  
Esterified Fatty Acids ◽  
Increased Risk ◽  
Significant Impairment

Elevated circulating levels of NEFAs (non-esterified fatty acids) are associated with states of insulin resistance and increased risk of vascular disease. Previous animal and human studies have demonstrated NEFA-induced endothelial dysfunction of large conduit arteries, reversible by the antioxidant ascorbic acid. We therefore investigated the effect of NEFAs on carbachol-induced endothelium-dependent vasodilation of rat resistance arteries in vitro using the technique of wire myography. In addition, we investigated the effect of co-incubation of NEFAs and ascorbic acid. Cumulative concentration–response curves to carbachol (endothelium-dependent vasodilation) and SNAP (S-nitroso-N-acetyl-DL-penicillamine; endothelium-independent vasodilation) were constructed. Those to carbachol were repeated following a 30 min incubation with either oleic acid (10−4 M) or palmitic acid (10−4 M), demonstrating significant impairment of endothelium-dependent vasodilation with both [P<0.05, comparison of pD2 values (the negative log concentration of agonist required to effect a 50% response)]. A cumulative concentration–response curve to carbachol was repeated following co-incubation with palmitic acid (10−4 M) and the antioxidant ascorbic acid (10−5 M), demonstrating an abolition of the previously observed endothelial dysfunction induced by palmitic acid. There was no impairment of vasodilation to SNAP following NEFA incubation. We conclude that NEFAs directly impair endothelial function in rat resistance arteries via an increase in oxidative stress at the vascular endothelium.

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