Fishmeal hydrolysation and non-protein energy sources affect the kinetics of nutrient digestion in the gastrointestinal tract of African catfish (Clarias gariepinus)

The article presents specific features of adaptive response of stomach tissues of African sharptooth catfish bred in artificial environment with application of “Sporothermin” probiotic. Probiotics influence metabolism of the host organism due to their participation in digestion processes, they also promote synthesis of proteins and increase the degree of absorption. The microorganisms that compose the probiotic contribute to better assimilation of incoming nutrients into the fish organism, they synthesize biologically active substances due to their fermentation activity. Histological slides were analyzed and documented applying a universal microscope Axio Imager.M2. Differences were found in structural features of the stomach of fish bred with application of “Sporothermin” probiotic and without it. Thus, fish that did not receive the probiotic had edema of the stomach inner muscular layer, epithelial layer detachment from the gastric mucosa and disorder of structural organization of the gastric fields. These changes were not observed in fish bred with application of “Sporothermine” probiotic. The conducted studies indicate the prospects of using the microbiota with probiotic properties when breeding Clarias gariepinus in an artificial environment. The spore forms of bacteria (Bacillus subtilis, Bacillus licheniformis), which are part of the probiotic “Sporothermin”, contribute to improvement of microbiocenosis of fish gastrointestinal tract, triggering a mechanism that stimulates a complex of adaptive reactions in stomach tissues of Clarias gariepinus. At the same time, conditionally pathogenic and pathogenic microbiota in the gastrointestinal tract is suppressed, ensuring effective digestion and increasing the degree of nutrient absorption. As a result, the productivity of the fish stock increases and the costs of breeding it in an artificial environment are reduced.

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EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

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