ADAPTIVE RESPONSE OF STOMACH TISSUES OF AFRICAN CATFISH TO MICROBIOTA WITH PROBIOTIC PROPERTIES

The article presents specific features of adaptive response of stomach tissues of African sharptooth catfish bred in artificial environment with application of “Sporothermin” probiotic. Probiotics influence metabolism of the host organism due to their participation in digestion processes, they also promote synthesis of proteins and increase the degree of absorption. The microorganisms that compose the probiotic contribute to better assimilation of incoming nutrients into the fish organism, they synthesize biologically active substances due to their fermentation activity. Histological slides were analyzed and documented applying a universal microscope Axio Imager.M2. Differences were found in structural features of the stomach of fish bred with application of “Sporothermin” probiotic and without it. Thus, fish that did not receive the probiotic had edema of the stomach inner muscular layer, epithelial layer detachment from the gastric mucosa and disorder of structural organization of the gastric fields. These changes were not observed in fish bred with application of “Sporothermine” probiotic. The conducted studies indicate the prospects of using the microbiota with probiotic properties when breeding Clarias gariepinus in an artificial environment. The spore forms of bacteria (Bacillus subtilis, Bacillus licheniformis), which are part of the probiotic “Sporothermin”, contribute to improvement of microbiocenosis of fish gastrointestinal tract, triggering a mechanism that stimulates a complex of adaptive reactions in stomach tissues of Clarias gariepinus. At the same time, conditionally pathogenic and pathogenic microbiota in the gastrointestinal tract is suppressed, ensuring effective digestion and increasing the degree of nutrient absorption. As a result, the productivity of the fish stock increases and the costs of breeding it in an artificial environment are reduced.

Download Full-text

Comparison of fatty acids, cholesterol, fat soluble vitamins and carotenoids content of skin and edible tissue of farmed African catfish (Clarias gariepinus, Burchell 1822)

Ovidius University Annals of Chemistry ◽

10.2478/auoc-2018-0006 ◽

2018 ◽

Vol 29 (1) ◽

pp. 41-47 ◽

Cited By ~ 1

Author(s):

Albena Merdzhanova ◽

Diana A. Dobreva ◽

Veselina Panayotova

Keyword(s):

Fatty Acids ◽

Lipid Extraction ◽

Clarias Gariepinus ◽

Dietary Lipids ◽

Biologically Active ◽

African Catfish ◽

Active Components ◽

Edible Tissue ◽

Fat Soluble Vitamins

Abstract African catfish (Clarias gariepinus) is new species for the Bulgarian market. It is a valuable source of biologically active components that play an important role in human diet, but there is lack of information for the quality of its dietary lipids. This study focuses on the assessment of skin and edible tissue lipid quality of farmed African catfish based on lipid content and detailed fatty acids, fat-soluble vitamins, cholesterol and carotenoids composition. Fatty acid composition was determined by gas chromatography with mass spectrometer (GC/MS) after lipid extraction. Vitamins A, D3 and E, beta-carotene, astaxanthin and cholesterol were analyzed simultaneously using high performance liquid chromatography (HPLC) with ultraviolet and fluorescence detectors. Lipids, cholesterol, astaxanthin and monounsaturated fatty acids (MUFA) were significantly higher in skin, whereas vitamin A and E, polyunsaturated fatty acids (PUFA) were higher in muscle tissue. Vitamin D3 showed comparable amounts in both tissues. Eicosapentaenoic acid (C20:5n3) and docosahexaenoic acid (C22:6n3) which are important indicators for fish lipids quality presented significantly high amounts. A portion of 100 g filet without skin contains approximately 600 mg. Results confirmed that African catfish meat - with or without the skin, can be valuable and preferable source of biologically active lipids.

Download Full-text

MICROARCHITECTONICS OF FEMALE SHARPTOOTH CATFISH GONADS GROWN IN AN ARTIFICIAL ENVIRONMENT

Vestnik of Ulyanovsk state agricultural academy ◽

10.18286/1816-4501-2020-4-154-160 ◽

2020 ◽

pp. 154-160

Author(s):

Е. V. Spirina ◽

◽

Е. М. Romanova ◽

V. N. Lyubomirova ◽

D. А. Kharitonov ◽

...

Keyword(s):

Clarias Gariepinus ◽

Growth Period ◽

Control Group ◽

Histological Structure ◽

Bearing Plate ◽

Light Yellow ◽

Universal Microscope ◽

Artificial Environment ◽

African Sharptooth Catfish

The article presents the research results obtained by use of probiotic «Sporothermin» for the cultivation of females of African sharptooth catfish in an artificial environment. Sporothermin is a probiotic that is a mixture of Bacillus subtilis and Bacillus licheniformis, which provides an increase in the adaptive abilities of fish to the long-term action of various adverse factors, which is relevant when growing in an artificial environment. The histological structure of the gonads of female sharptooth catfish was analyzed using a research motorized universal microscope Axio Imager. M2 (Carl Zeiss, Germany). The results of our research have shown that in sharptooth catfish grown using the probiotic «Sporothermin», germ cells are observed, represented by oogonia and young oocytes of the protoplasmic growth period. Oocytes appear, that finished protoplasmic growth as well as oocytes that have completed trophoplasmic growth. At this stage, oocytes in the cytoplasm contain fat droplets that are part of the yolk grains, coloring from light yellow to orange with various shades. Female sharptooth catfish (Clarias gariepinus) of the control group, grown without the use of the probiotic «Sporothermin», have a violation of the synthesis of yolk granules, as well as edema of the follicular layer of the gonads, resorption of yolk grains and edema of the connective tissue egg-bearing plate, there is no striation of the radial layer, and deformation of the trophoplasmic oocyte is noted. In histological studies of the gonads of female sharptooth catfish, it was found that the use of the probiotic «Sporothermin» leads to a decrease in disorders in their microstructure, due to the strengthening of the membranes of the gonad cells.

Download Full-text

Bio-accumulation of selected metals in African sharptooth catfish Clarias gariepinus from the lower Olifants River, Mpumalanga, South Africa

Koedoe ◽

10.4102/koedoe.v40i1.265 ◽

1997 ◽

Vol 40 (1) ◽

Cited By ~ 7

Author(s):

H.H. Du Preez ◽

M. Van der Merwe ◽

J.H.J. Van Vuren

Keyword(s):

National Park ◽

Anthropogenic Activities ◽

Clarias Gariepinus ◽

Kruger National Park ◽

African Catfish ◽

Diffuse Sources ◽

Metal Levels ◽

Olifants River ◽

African Sharptooth Catfish ◽

Kidney Liver

The level of metal (Cr, Cu, Mn, Ni, Pb and Zn) bio- accumulation in tissues (muscle, gill, kidney, liver and gonads) and bile of the African catfish Clarias gariepinus, from the lower Olifants River was investigated. These metals were detected in all the tissues as well as in the bile, with the highest concentration found in either the gills, liver or gonadal tissue. The lowest concentration was usually detected in the muscle tissue. Although statistic comparisons revealed no significant differences between the localities, fish from the Selati River (Locality 1) generally had higher metal levels than fish from the localities along the Olifants River inside the Kruger National Park. The higher levels in the fish from the Selati River may be attributed to anthropogenic activities resulting in point and/or diffuse sources of metal pollution. These sources should be identified and reduced.

Download Full-text

Fishmeal hydrolysation and non-protein energy sources affect the kinetics of nutrient digestion in the gastrointestinal tract of African catfish (Clarias gariepinus)

Aquaculture ◽

10.1016/j.aquaculture.2021.737425 ◽

2021 ◽

pp. 737425

Author(s):

F.E. Elesho ◽

D.A.H. Sutter ◽

R. Frenken ◽

J.A.J. Verreth ◽

S. Kröckel ◽

...

Keyword(s):

Gastrointestinal Tract ◽

Clarias Gariepinus ◽

Energy Sources ◽

African Catfish ◽

Nutrient Digestion ◽

Protein Energy ◽

Kinetics Of

Download Full-text

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Download Full-text

PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

Download Full-text

On the diagnostics of Adonis L. species (Ranunculaceae) of the Russian flora

10.33920/med-06-2002-01 ◽

2020 ◽

pp. 3-14

Author(s):

Aleksandr Luferov

Keyword(s):

Raw Materials ◽

Natural Populations ◽

Structural Features ◽

Biologically Active ◽

Scientific Medicine ◽

Diagnostic Features ◽

Plant Raw Materials ◽

Similar Chemical ◽

Chemical Studies ◽

Alternative Sources

The article provides brief information about cardiotonic, sedative, cytostatic, diuretic, and antibacterial effects of biologically active compounds of Adonis L. (Ranunculaceae) species. Chemical studies allowed to identify the cardiac glycosides, or cardenolides: or cardenolides: adontoxin, adonitol, adonitoxigenin, acetyldigitoxin and others. In scientific medicine, it is currently allowed to use Adonis vernalis L. Other types of Adonis have a similar chemical composition and are offered as substitutes for this official species, for example, Adonis apennina L. Many Adonis species have limited natural resources, and in some regions are rare, requiring conservation of their natural populations. The search for alternative sources of medicinal plant raw materials, based on this, is relevant. The experimental part of our research was carried out using the morphological and geographical method with the involvement of information on ecology and phenology. For the first time summarizes the diagnostic features of Adonis flora of Russian flora. Previously unknown structural features (shape and size of anthers) were identified that characterize the subgenera Adonanthe and Adonis. Taxonomic study of the genus Adonis of the Russian flora allowed us to determine its species composition, clarify its systematic affiliation, and nomenclature synonyms. 9 species were identified. Of these, 6 are perennials belonging to the subgenus Adonanthe, section Consiligo, which includes 2 subsections: Amurenses (2 species) and Vernales, which is differentiated into 2 rows: Apenninae (2 species) and Vernales (2 species). Subgenus Adonis is represented by 2 sections: Adonis (1 species) and Lophocarpa with sections Aestivales (1 species) and Dentatae (1 species). For all the considered species and varieties, the main distribution areas are given. A key has been compiled to determine the wild Adonis species distributed in Russia.

Download Full-text