Structural analysis of cysteine-free Nt.BspD6 nicking endonuclease and its functional features

2022 ◽  
pp. 140756
Author(s):  
Rimma I. Artyukh ◽  
Bulat F. Fatkhullin ◽  
Galina S. Kachalova ◽  
Valeriya N. Antipova ◽  
Tatyana A. Perevyazova ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Nicking Endonuclease ◽  
Functional Features

scholarly journals Текстуализация эпических текстов и формирование функциональных глав: текстологический анализ песни о воскрешении богатырей в монгольском «Гэсэре»

2020 ◽  
Vol 16 (4) ◽  
pp. 200-212
Author(s):  
Yu Lan ◽  
◽  
Tsagan B. Seleeva
Keyword(s):  
Comparative Analysis ◽  
Structural Analysis ◽  
Unique Function ◽  
Logical Function ◽  
Epic Tradition ◽  
Atypical Features ◽  
Heroic Epic ◽  
Functional Features

The Mongolian “Geser” is an epic cycle consisting of many songs, which originally consisted of one or more chapters. In the process of forming the cycle, some chapters acquire structural and functional features that differ from traditional Mongolian epics, and these atypical features characterize the uniqueness of the process of formation and functioning of some chapters of the epic. One of these texts with atypical features in the structure, theme and plot is the chapter on the resurrection of the heroes of Gesar. In this article, on the basis of structural analysis, a comparative analysis of this chapter with the text of the Mongolian historical chronicle “Altan Tobchi” is given. It is assumed that the formation of an atypical structure of this chapter is focused on its unique function, that is, there is a transformation of the traditional functions of both the narrative and lyrical parts of this chapter. The narrative part has the discursive function of connecting other independent chapters and the logical function of connecting “Gesar” with the Mongolian heroic epic tradition, while the lyrical part reveals the independence and heroism of the plot, thereby allowing the simple plot to become an independent heroic epic chapter.

scholarly journals COMPONENT-STRUCTURAL ANALYSIS OF LEADERSHIP POTENTIAL OF PRESCHOOLERS

2020 ◽  
Vol 11 (87) ◽  
Author(s):  
Olga Bessonova ◽  
◽  
Keyword(s):  
Structural Analysis ◽  
Personal Characteristics ◽  
Problem Situation ◽  
Creative Activity ◽  
Leadership Qualities ◽  
Leadership Potential ◽  
Activity Component ◽  
Communication Ability ◽  
Functional Features ◽  
The Individual

The article presents the results of a theoretical study of the problem of component-structural analysis of leadership potential in relation to preschool children, in particular, analyzed the approaches of scientists to understanding the concept of «leadership potential of preschoolers» and its structural components. We support the opinion of scientists in understanding this phenomenon as a set of psychological qualities that would meet the needs of the group and are most useful for solving the problem situation in which this group found itself. During the structural-component analysis of the concept of «leadership potential of the preschooler» the personal characteristics inherent in the leader (intellectual, volitional, general, ideological, value, organizational) and emotional-value attitude (ideals, values), his role in determining the purpose and orientation of group actions (ability to establish interpersonal interaction and communication, ability to adapt behavior), functional features of the leader's interaction with group members in real situations (motivation, creativity and reflection) and universal (experience, skills, leadership styles, motives, introspection, reflection) components. The individual component is transformed into a personal component, and the universal component - into social and creative-activity components. Based on the generalization of approaches to the components of leadership potential of the individual, in order to further substantiate the criterion apparatus we have developed a structure of leadership potential of the individual, according to which it contains three interrelated components: activities (indicators: personal leadership qualities, emotional and value attitude); the social component is explained by the desire to use leadership potential for the benefit of the group (indicators: communication, partnership, adaptive behavior); creative-activity component is considered as a technological block of leadership potential and is a manifestation of readiness for its practical use in specific situations of interaction (motivation to succeed, creative approach, ability to reflect).

Structural analysis of the surface-layer protein of spirillum serpens by high-resolution electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 738-739
Author(s):  
W. H. Wu ◽  
R. M. Glaeser
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Structural Analysis ◽  
High Resolution ◽  
Low Dose ◽  
High Resolution Electron Microscopy ◽  
Optical Diffraction ◽  
Surface Layer Protein ◽  
Morphological Unit ◽  
Resolution Electron

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.

RNA polymerase: Preparation and structural analysis of helical polymers

1984 ◽  
Vol 42 ◽  
pp. 152-153
Author(s):  
E. Loren Buhle ◽  
Pamela Rew ◽  
Ueli Aebi
Keyword(s):  
Structural Analysis ◽  
Rna Polymerase ◽  
Molecular Level ◽  
X Ray Diffraction ◽  
Helical Polymers ◽  
X Ray ◽  
E Coli ◽  
The Past ◽  
Ordered Arrays ◽  
Low Ionic Strength

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.

Interpreting HVEM of muscle-cell impulse networks

1992 ◽  
Vol 50 (1) ◽  
pp. 126-127
Author(s):  
Paul DeCosta ◽  
Kyugon Cho ◽  
Stephen Shemlon ◽  
Heesung Jun ◽  
Stanley M. Dunn
Keyword(s):  
Structural Analysis ◽  
Muscle Cell ◽  
Electron Micrographs ◽  
Relative Size ◽  
Automatic Classification ◽  
Nerve Fibers ◽  
Analysis Algorithm ◽  
Structural Networks

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).

Structural analysis of a binary metallic glass model. I. — The Pd80Si20 alloy

1985 ◽  
Vol 46 (2) ◽  
pp. 235-241 ◽  
Author(s):  
F. Lançon ◽  
L. Billard ◽  
J. Laugier ◽  
A. Chamberod
Keyword(s):  
Structural Analysis ◽  
Metallic Glass ◽  
Glass Model

SYNCHROTRON RADIATION AS A SOURCE FOR STRUCTURAL ANALYSIS IN BIOLOGY

1973 ◽  
Vol 34 (C8) ◽  
pp. C8-63-C8-63
Author(s):  
J. BARRINGTON LEIGH ◽  
G. ROSENBAUM
Keyword(s):  
Structural Analysis ◽  
Synchrotron Radiation
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