scholarly journals Ginkgolide B treatment regulated intestinal flora to improve high-fat diet induced atherosclerosis in ApoE−/− mice

2021 ◽  
Vol 134 ◽  
pp. 111100
Author(s):  
Zhiyang Lv ◽  
Xin Shan ◽  
Qingbo Tu ◽  
Jie Wang ◽  
Jing Chen ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Intestinal Flora ◽  
Ginkgolide B ◽  
High Fat

scholarly journals Saturated hydrogen improves lipid metabolism disorders and dysbacteriosis induced by a high-fat diet

2020 ◽  
Vol 245 (6) ◽  
pp. 512-521 ◽  
Author(s):  
Xiangjie Qiu ◽  
Qiaona Ye ◽  
Mengxing Sun ◽  
Lili Wang ◽  
Yurong Tan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
High Fat Diet ◽  
Metabolic Disorders ◽  
Metabolic Diseases ◽  
Isocitrate Lyase ◽  
Intestinal Flora ◽  
Glyoxylic Acid ◽  
Density Lipoprotein ◽  
High Fat ◽  
Acid Cycle

Studies have shown that metabolic diseases, such as obesity, are significantly associated with intestinal flora imbalance. The amplification of opportunistic pathogens induced by the glyoxylic acid cycle contributes to intestinal flora imbalance. Promising, though, is that saturated hydrogen can effectively improve the occurrence and development of metabolic diseases, such as obesity. However, the specific mechanism of how saturated hydrogen operates is still not very clear. In this study, after a high-fat diet, the level of total cholesterol, total glyceride, and low-density lipoprotein in the peripheral blood of mice increased, and that of high-density lipoprotein decreased. Intestinal fatty acid metabolism-related gene Apolipoprotein E (ApoE), fatty acid synthase (FAS), intestinal fatty acid-binding protein (I-FAPB), acetyl-CoA carboxylase 1 (ACC1), peroxisome proliferator-activated receptor γ (PPARγ), and stearoyl-CoA desaturase 1 (SCD1) increased significantly. Bacteroides, Bifidobacteria, and Lactobacillus counts in feces decreased considerably, while Enterobacter cloacae increased. The activity of isocitrate lyase in feces increased markedly. Treatment of mice with saturated hydrogen led to decreased total cholesterol, total glyceride, and low-density lipoprotein and increased high-density lipoprotein in the peripheral blood. FAS and I-FAPB gene expression in the small intestine decreased. Bacteroides, Bifidobacteria, and Lactobacillus in feces increased significantly, whereas Enterobacter cloacae decreased. The activity of isocitrate lyase also diminished remarkably. These results suggest that saturated hydrogen could improve intestinal structural integrity and lipid metabolism disorders by inhibiting the glyoxylic acid cycle of the intestinal flora. Impact statement Past studies have shown that hydrogen can improve metabolic disorders, but its mechanism of action remains unclear. It is well known that metabolic diseases, such as obesity, are significantly associated with changes in the intestinal flora. The glyoxylic acid cycle is an essential metabolic pathway in prokaryotes, lower eukaryotes, and plants and could be the portal for mechanisms related to metabolic disorders. Many opportunistic pathogenic bacteria can recycle fatty acids to synthesize sugars and other pathogenic substances using the glyoxylic acid cycle. So, the glyoxylic acid cycle may be involved in intestinal dysbacteriosis under high-fat diet. This study, therefore, seeks to provide the mechanism of how hydrogen improves metabolic diseases and a new basis for the use of hydrogen in the treatment of metabolic disorders.

scholarly journals Ginkgolide B ameliorates high-fat diet-induced hepatic steatosis and inflammation in mice

2020 ◽  
Vol 28 (13) ◽  
pp. 519-525 ◽  
Author(s):  
Si-Qin Long ◽  
Xiu-Feng Yu ◽  
Yu-Li Ge ◽  
Lu-Xiang Liu
Keyword(s):  
Hepatic Steatosis ◽  
High Fat Diet ◽  
Ginkgolide B ◽  
High Fat

Sesamin Modulation of Intestinal Microflora in Rats on High-Fat Diet

2020 ◽  
Vol 19 (3) ◽  
pp. 312-316
Author(s):  
Pan Liu ◽  
Yu Deng ◽  
Mengxi Wang ◽  
Lingyan Kong ◽  
Na Xu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Intestinal Microflora ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Short Chain Fatty Acids ◽  
Sequencing Analysis ◽  
Anaerobic Culture ◽  
High Fat ◽  
E Coli

The present study aimed to investigate the effects of sesamin on intestinal flora in rats on high-fat diet by in vitro animal fecal anaerobic culture system. Results showed that treatment with sesamin increased the abundance of Lactobacillus acidophilus and inhibited the growth of E. coli. High throughput sequencing analysis showed that treatment with sesamin increased the abundance of probiotics such as Lactobacillus and Flavonifractor, and decreased the abundance of harmful bacteria such as Bacteroides, Enterococcus, Clostridium_XlVa, and Parabacteroides. Moreover, treatment with sesamin increased the concentration of total short chain fatty acids (acetic acid and isovaleric acid). In conclusion, sesamin intervention improved the composition and abundance of intestinal microorganisms of high fat diet rats, which would be beneficial to intestinal health.

Luteolin cooperated with metformin hydrochloride alleviates lipid metabolism disorders and optimizes intestinal flora compositions of high-fat diet mice

2020 ◽  
Vol 11 (11) ◽  
pp. 10033-10046
Author(s):  
Xiaodong Ge ◽  
Chang'e Wang ◽  
Huiling Chen ◽  
Tingting Liu ◽  
Ligen Chen ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Signaling Pathways ◽  
High Fat Diet ◽  
Intestinal Flora ◽  
Metformin Hydrochloride ◽  
High Fat ◽  
Lipid Metabolism Disorders

LU·MH regulates lipid metabolism signaling pathways and intestinal flora to alleviate lipid metabolism disorders of high-fat diet mice.

scholarly journals Exploring the Mechanism of Action of Canmei Formula Against Colorectal Adenoma Through Multi-Omics Technique

2021 ◽  
Vol 9 ◽  
Author(s):  
Cui Guo ◽  
Xiaoqiang Liu ◽  
Yimin Xu ◽  
Xinyue Han ◽  
Runnan Xie ◽  
...  
Keyword(s):  
Treatment Group ◽  
Colorectal Adenoma ◽  
Mechanism Of Action ◽  
High Fat Diet ◽  
Intestinal Flora ◽  
The Other ◽  
High Fat ◽  
Blank Group ◽  
Inorganic Pyrophosphate ◽  
Prevention And Treatment

Background: Canmei formula (CMF) is a traditional Chinese medicine compound with definite effect on the prevention and treatment of colorectal adenoma (CRA). CMF can prevent the transformation of intestinal inflammation to cancer. This study explored the mechanism of action of CMF in anti-CRA using multi-omics techniques.Method: The mice were randomly divided into four groups: blank group (Control), high-fat diet (HFD) + AOM/DSS colorectal adenoma model (ADH) groups, Canmei formula treatment group (ADH-CMF) and sulfasalazine treatment group (Sul). Except for the blank group, ADH model was established in the other three groups by intraperitoneal injection with AOM reagent, and then mice were given 2.5% DSS in free drinking water and high-fat diet. The mice in the blank group and ADH groups were intragastrically perfused with normal saline, and the mice in the other two groups were treated with corresponding drugs for 20 weeks. During this period, the changes of physical signs of mice in each group were observed. The differentially expressed genes and proteins in the Control group, ADH group and ADH-CMF group were detected by RNA-seq transcriptome sequencing and Tandem Mass Tags (TMT) quantitative proteomics. After the combined analysis and verification, the key targets were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Moreover, the changes of intestinal flora in mice of the three groups were examined.Results: A total of 2,548 differential genes were obtained by transcriptomics analysis, and 45 differential proteins were obtained by proteomics analysis. The results of proteomics data and experimental verification showed that CMF mainly affected the Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase (LHPP) target. GO analysis showed that the targets of CMF were involved in the biological processes such as cellular process, metabolic process and biological regulation. KEGG analysis showed that those genes were involved in oxidative phosphorylation, cell senescence, and metabolic pathways. Studies have shown that LHPP overexpression impeded colorectal cancer cell growth and proliferation in vitro, and was associated with a change in PI3K/AKT activity. The results of 16S DNA high-throughput sequencing showed that CMF could effectively regulate the abundance of Bifidobacterium, Candidatus_Saccharimonas and Erysipelatoclostridium in the intestinal flora at the genus level.Conclusion: CMF regulates LHPP via the PI3K/AKT signaling pathway. CMF affects the abundance of specific intestinal flora and can regulate the disorder of intestinal flora to achieve the role of prevention and treatment of CRA.

scholarly journals Effects of Banana Resistant Starch on the Biochemical Indexes and Intestinal Flora of Obese Rats Induced by a High-Fat Diet and Their Correlation Analysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Jinfeng Fu ◽  
Yuting Wang ◽  
Simin Tan ◽  
Juan Wang
Keyword(s):  
Weight Loss ◽  
Resistant Starch ◽  
High Fat Diet ◽  
Low Density Lipoprotein ◽  
Intestinal Flora ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
High Fat ◽  
Obese Rats ◽  
Level 3

The effects of banana resistant starch (BRS) on obesity-related metabolic and intestinal flora were investigated in a high-fat diet-induced obesity model. After 6 weeks of intervention, the glucolipid metabolism index [blood glucose (GLU), total cholesterol (TC), triacylglycerol (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C)], hormone index [leptin (LEP), insulin (INS), ghrelin, adiponectin (ADP), and thyroxine (T4)], and 16S rRNA sequencing analyses were performed for each group to explore the regulating effect of intestinal flora and the mechanism of weight loss in obese rats. The results showed that (1) BRS intervention significantly reduced the levels of GLU, TG, TC, LDL-C, LEP, and INS (p < 0.01) and increased the contents of ghrelin (p < 0.05) and ADP (p < 0.01). (2) BRS could improve the diversity of intestinal flora and regulate the overall structure of intestinal microorganisms, mainly by upregulating the Bacteroides/Firmicutes ratio and the relative abundance of Cyanobacteria and downregulating the relative abundances of Deferribacteres and Tenericutes (at the phylum level). BRS could inhibit the proliferation of Turicibacter, Romboutsia, and Oligella and increase the abundances of Bacteroides, Ruminococcaceae, and Lachnospiraceae (at the genus level). (3) Some significant correlations were observed between the gut microbiota and biomarkers. Turicibacter, Romboutsia, and Oligella were positively correlated with GLU, TG, TC, LEP, and INS and negatively correlated with ghrelin and ADP. Bacteroides, Parabacteroides, and Akkermansia were negatively correlated with GLU, TG, and TC. Conclusion: BRS had promising effects on weight loss, which could be associated with the improvement in host metabolism by regulating intestinal flora.

scholarly journals Gegen Qinlian Decoction Attenuates High-Fat Diet-Induced Steatohepatitis in Rats via Gut Microbiota

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Yi Guo ◽  
Pang-hua Ding ◽  
Li-juan Liu ◽  
Lei Shi ◽  
Tang-you Mao ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Group Treatment ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
Intestinal Flora ◽  
Underlying Mechanism ◽  
Control Group ◽  
High Fat ◽  
Nonalcoholic Fatty Liver ◽  
Traditional Chinese Herbal Medicine

Gut microbiota play an important role in modulating energy contribution, metabolism, and inflammation, and disruption of the microbiome population is closely associated with chronic metabolic diseases, such as nonalcoholic fatty liver disease (NAFLD). Gegen Qinlian decoction (GGQLD), a well-known traditional Chinese herbal medicine (CHM), was previously found to regulate lipid metabolism and attenuate inflammation during NAFLD pathogenesis. However, the underlying mechanism of this process, as well as how the gut microbiome is involved, remains largely unknown. In this study, we investigated the effect of varying doses of GGQLD on the total amount and distribution of gut bacteria in rats fed a high-fat diet (HFD) for 8 weeks. Our analysis indicates that Oscillibacter and Ruminococcaceae_g_unclassified are the dominant families in the HFD group. Further, HFD-dependent differences at the phylum, class, and genus levels appear to lead to dysbiosis, characterized by an increase in the Firmicutes/Bacteroidetes ratio and a dramatic increase in the Oscillibacter genus compared to the control group. Treatment with GGQLD, especially the GGQLL dose, improved these HFD-induced changes in intestinal flora, leading to increased levels of Firmicutes, Clostridia, Lactobacillus, bacilli, and Erysipelotrichales that were similar to the controls. Taken together, our data highlight the efficacy of GGQLD in treating NAFLD and support its clinical use as a treatment for NAFLD/NASH patients.

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