Lipid extraction methods from microalgal biomass harvested by two different paths: Screening studies toward biodiesel production

2013 ◽  
Vol 133 ◽  
pp. 378-388 ◽  
Author(s):  
Sergio D. Ríos ◽  
Joandiet Castañeda ◽  
Carles Torras ◽  
Xavier Farriol ◽  
Joan Salvadó
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Microalgal Biomass

scholarly journals Physical Methods of Microalgal Biomass Pretreatment

2014 ◽  
Vol 28 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Agata Piasecka ◽  
Izabela Krzemińska ◽  
Jerzy Tys
Keyword(s):  
Alternative Energy ◽  
Lipid Extraction ◽  
Physical Method ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Biomass Pretreatment ◽  
Microalgal Biomass ◽  
Alternative Energy Sources ◽  
Wet Biomass ◽  
Microwave Techniques

Abstract The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

scholarly journals Influence of Light Intensity and Photoperiod on the Photoautotrophic Growth and Lipid Content of the Microalgae Verrucodesmus verrucosus in a Photobioreactor

2021 ◽  
Vol 13 (12) ◽  
pp. 6606
Author(s):  
Laura Vélez-Landa ◽  
Héctor Ricardo Hernández-De León ◽  
Yolanda Del Carmen Pérez-Luna ◽  
Sabino Velázquez-Trujillo ◽  
Joel Moreira-Acosta ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Accumulation ◽  
Growth Parameters ◽  
Methyl Esters ◽  
Lipid Extraction ◽  
Growth Conditions ◽  
Biodiesel Production ◽  
Microalgal Biomass ◽  
Bg11 Medium ◽  
Gas Chromatography Mass Spectroscopy

Microalgal biomass has the capacity to accumulate relatively large quantities of triacylglycerides (TAG) for the conversion of methyl esters of fatty acids (FAME) which has made microalgae a desirable alternative for the production of biofuels. In the present work Verrucodesmus verrucosus was evaluated under autotrophic growth conditions as a suitable source of oil for biodiesel production. For this purpose BG11 media were evaluated in three different light:dark photoperiods (L:D; 16:08; 12:12; 24:0) and light intensities (1000, 2000 and 3000 Lux) in a photobioreactor with a capacity of three liters; the evaluation of the microalgal biomass was carried out through the cell count with the use of the Neubauer chamber followed by the evaluation of the kinetic growth parameters. So, the lipid accumulation was determined through the lipid extraction with a Soxhlet system. Finally, the fatty acid profile of the total pooled lipids was determined using gas chromatography-mass spectroscopy (GC-MS). The results demonstrate that the best conditions are a photoperiod of 12 light hours and 12 dark hours with BG11 medium in a 3 L tubular photobioreactor with 0.3% CO2, 25 °C and 2000 Lux, allowing a lipid accumulation of 50.42%. Palmitic acid is identified as the most abundant fatty acid at 44.90%.

scholarly journals Biodiesel production from microalgae by enzymatic transesterification

2015 ◽  
Author(s):  
◽  
Abhishek Guldhe
Keyword(s):  
Energy Consumption ◽  
Production Process ◽  
Lipid Extraction ◽  
International Standards ◽  
Biodiesel Production ◽  
Enzymatic Conversion ◽  
Microalgal Biomass ◽  
Whole Cell ◽  
Immobilized Lipases ◽  
Microalgal Lipids

Main focus of this study is to investigate the enzymatic-conversion of microalgal lipids to biodiesel. However, preceding steps before conversion such as drying of microalgal biomass and extraction of lipids were also studied. Downstream processing of microalgae has several challenges and there is very little literature available in this area. S. obliquus was grown in the pilot scale open pond cultivation system for biomass production. Different techniques were studied for biomass drying and extraction of lipids from harvested microalgal biomass. Effect of these drying and extraction techniques on lipid yield and quality was assessed. Energy consumption and economic evaluation was also studied. Enzymatic conversion of microalgal lipids by extracellular and whole cell lipase application was investigated. For both applications, free and immobilized lipases from different sources were screened and selected based on biodiesel conversion. Process parameters were optimized using chosen extracellular and whole cell lipases; also step-wise methanol addition was studied to improve the biodiesel conversion. Immobilized lipase was studied for its reuse. Final biodiesel was characterized for its fuel properties and compared with the specifications given by international standards. Enzymatic conversion of microalgal lipids was compared with the conventional homogeneous acid-catalyzed conversion. Enzymatic conversion and chemical conversion were techno-economically investigated based on process cost, energy consumption and processing steps. Freeze drying was the most efficient technique, however at large scale economical sun drying could also be selected as possible drying step. Microwave assisted lipid extraction performed better compared to sonication technique. Immobilized P. fluorescens lipase in extracellular application and A. niger lipase in whole cell application showed superior biodiesel conversion. The extracellular immobilized P. fluorescens lipase showed better biodiesel conversion and yields than the immobilized A. niger whole cell lipase. Both the enzyme catalysts showed lower biodiesel conversion compared to conventional chemical catalyst and higher processing cost. However, techno-economic analysis showed that, the reuse potential of immobilized lipases can significantly improve the economics. Fewer purification steps, less wastewater generation and minimal energy input are the benefits of enzymatic route of biodiesel conversion. Microalgae as a feedstock and lipase as a catalyst for conversion makes overall biodiesel production process environmentally-friendly. Data from this study has academic as well as industrial significance. Conclusions from this study form the basis for greener and sustainable scaling-up of microalgal biodiesel production process.

scholarly journals Optimization of cell wall disruption and lipid extraction methods by combining different solvents from wet microalgae

2021 ◽  
Author(s):  
Daryush Arabian
Keyword(s):  
Cell Wall ◽  
Lipid Extraction ◽  
Dry Weight ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Cell Wall Disruption ◽  
Bead Mill ◽  
Wet Microalgae ◽  
Disruption Method ◽  
Chloroform Methanol

Microalgae have emerged as one of the most promising options for biodiesel production over the past few decades. Lipid extraction from microalgae for biodiesel production as a bottleneck of biodiesel production technology was the main purpose of this study. In this study different methods of the cell wall disruption were compared. Then, two methods of ultrasound and bead mill were used as methods of the cell wall disruption. The maximum lipid extracted by ultrasound was 17.10% and by bead mill was 15.16% (based on microalgae biomass dry weight). After the cell wall disruption of microalgae, for lipid extraction, chloroform-methanol solvent combination was used as a high extraction method and hexane-ethanol solvent combination was used as an environmentally friendly method. In this regard, the effect of solvent to biomass ratio, temperature and extraction time was investigated and the optimal results for chloroform-methanol solvent combination were 8 ml/g, 45°C and 60 minutes, respectively, and for hexane-ethanol combination were 6 ml/g, 35◦C and 73 minutes, respectively. Under these optimal conditions, the highest amount of extracted lipid from Chlorella vulgaris with a moisture content of 87.50%, and ultrasound as a cell wall disruption method were obtained 20.39% and 16.41% (based on microalgae dry weight) with a combination of chloroform-methanol solvents and hexane-ethanol respectively. Also the highest extraction rates of 17.63% and 13.85% were obtained for the combination of chloroform-methanol and hexane-ethanol solvents, respectively by bead milling as cell wall disruption method

Biodiesel Production of Amphora sp. and Navicula sp. by Different Cell Disruption and Lipid Extraction Methods

2015 ◽  
Vol 9 (6) ◽  
pp. 588-595
Author(s):  
Haifa Chtourou ◽  
Ines Dahmen ◽  
Fatma Karray ◽  
Sami Sayadi ◽  
Abdelhafidh Dhouib
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Biodiesel Production

scholarly journals Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1928
Author(s):  
Cristina Blanco-Llamero ◽  
Paz García-García ◽  
Francisco Javier Señoráns
Keyword(s):  
Lipid Extraction ◽  
Hydrolytic Enzymes ◽  
Extraction Methods ◽  
Polar Lipids ◽  
Probe Type ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Microalgal Biomass ◽  
Nannochloropsis Gaditana ◽  
Neutral And Polar Lipids

Microalgal biomass is a sustainable source of bioactive lipids with omega-3 fatty acids. The efficient extraction of neutral and polar lipids from microalgae requires alternative extraction methods, frequently combined with biomass pretreatment. In this work, a combined ultrasound and enzymatic process using commercial enzymes Viscozyme, Celluclast, and Alcalase was optimized as a pretreatment method for Nannochloropsis gaditana, where the Folch method was used for lipid extraction. Significant differences were observed among the used enzymatic pretreatments, combined with ultrasound bath or probe-type sonication. To further optimize this method, ranges of temperatures (35, 45, and 55 °C) and pH (4, 5, and 8) were tested, and enzymes were combined at the best conditions. Subsequently, simultaneous use of three hydrolytic enzymes rendered oil yields of nearly 29%, showing a synergic effect. To compare enzymatic pretreatments, neutral and polar lipids distribution of Nannochloropsis was determined by HPLC–ELSD. The highest polar lipids content was achieved employing ultrasound-assisted enzymatic pretreatment (55 °C and 6 h), whereas the highest glycolipid (44.54%) and PE (2.91%) contents were achieved using Viscozyme versus other enzymes. The method was applied to other microalgae showing the potential of the optimized process as a practical alternative to produce valuable lipids for nutraceutical applications.

Sign in / Sign up

Export Citation Format

Share Document