Assessment of Chlorella vulgaris and indigenous microalgae biomass with treated wastewater as growth culture medium

2017 ◽  
Vol 244 ◽  
pp. 400-406 ◽  
Author(s):  
Luis C. Fernández-Linares ◽  
Claudia Guerrero Barajas ◽  
Enrique Durán Páramo ◽  
Jesús A. Badillo Corona
Keyword(s):  
Chlorella Vulgaris ◽  
Culture Medium ◽  
Treated Wastewater ◽  
Microalgae Biomass

Harmful cyanobacterial toxic blooms in waste stabilisation ponds

2000 ◽  
Vol 42 (10-11) ◽  
pp. 179-186 ◽  
Author(s):  
B. Oudra ◽  
M. El Andaloussi ◽  
S. Franca ◽  
P. Barros ◽  
R. Martins ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Health Hazard ◽  
Normal Growth ◽  
Treated Wastewater ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Diameter ◽  
Histopathological Study ◽  
Ecological Implication ◽  
Shellfish Poisoning ◽  
Mammalian Toxicity

A coccoid picocyanobacterium Synechocystis sp. (0.6-2 μm of cell diameter) was found to be dominant during summer period in the experimental wastewater stabilisation pond of Marrakesh. The taxonomy of this isolated strain was confirmed by electron microscope study. The general patterns of ultrastructure and the mode of cell division resemble Chroococcales. The cyanobacterium strain was axenic and cultured on both inorganic Z8 and BG13 media. Mammalian toxicity was confirmed by mice bioassay. The major sympton of poisoning was severe diarrhoea. Histopathological study shows a slight hepatotoxicosis associated with a pronounced change in the intestinal mucosa which shows swelling and destruction of villi epithelium and shedding of enterocytes into the lumen. Although slow, these kinds of poisoning are comparable to those induced by okadiac acid intraperitoneal mice injection (diarrhetic shellfish poisoning “DSP” toxins). By using the enzyme-linked immunosorbent assay (ELISA), the amount ofhepatotoxins “microcystins” was determined. The result shows that Synechocystis can produce a small amount of total microcystine [an average of 15 μg−1 dry weight corresponding to 20 ng(109cell)−1]. These findings lead us to consider Synechocystis as both a potent neurotoxin and hepatotoxin producer. Because of the confirmed cyanobacterium toxicity, an eventual ecological implication should be considered. However, a toxic chronic test experiment on Daphnia was simultaneously carried out. Juvenile D. magna (less than 24 hours old), were fed three concentrations (104, 106, 108 cells / ml) of Synechocystis. A group of organisms fed with Chlorella vulgaris (3. 105 cells/ml) and another group without food, were studied as control treatments. Only animals cultured with 104 cells/ml of cyanobacterium survived at 80% until the end of the test (21 days). Reproduction and normal growth occurred in control treatments fed with Chlorella vulgaris and the group fed with the lowest concentration of Synechocystis. One-way ANOVA statistical analyses show significant differences in Daphnia survival and growth, between treatments with and without Synechocystis and between treatments with and without food. In terms of this study, there is evidence that toxic picocyanobacteria blooms occurring in wastewater stabilization ponds of Marrakesh, could have harmful repercussions on zooplanktonic, bacteria and other algae communities. Consequently, this will constitute a possible hindrance for sewage self-purification process and system treatment performance. In addition, the reuse of such treated wastewater effluent for irrigation will constitute an additional, potent, health hazard for animals and human's.

Microalgae biomass and lipid production using primary treated wastewater

2017 ◽  
Vol 91 ◽  
pp. 228-234 ◽  
Author(s):  
Andriana F. Aravantinou ◽  
Eirini F. Barkonikou ◽  
Ioannis D. Manariotis
Keyword(s):  
Lipid Production ◽  
Treated Wastewater ◽  
Microalgae Biomass

scholarly journals Inexpensive and universal growth media for biomass production of microalgae

2019 ◽  
Vol 21 (1) ◽  
pp. 82-89 ◽  
Keyword(s):  
Chlorella Vulgaris ◽  
Algal Biomass ◽  
Large Scale ◽  
Sea Water ◽  
Production Costs ◽  
Growth Media ◽  
Growing Medium ◽  
Microalgae Biomass ◽  
Mixotrophic Conditions ◽  
Microalgae Growth

<p>New challenges for industrial microbiology and biotechnology of algae are to increase the efficiency of microalgae growth rates and decrease the cultivation costs. Algae could be cultivated in fresh water as well as in salty sea water or wastewater. Microalgae biomass can be used as a sorbent to remove microcontaminants (e.g. heavy metals, biogens) from wastewater. The obtained results showed that there is a possibility of application of a cheap and universal growing medium (Bf) despite common fertilizers: Bristol or BG-11 to cultivate Chlorella vulgaris and Scenedesmus armatus. The mixotrophic condition can be useful for cultivation of Chlorella vulgaris and Scenedesmus armatus. The highest concentrations of algal biomass for both species were determined after the application of the Bf medium, lower after the BG-11 medium and the lowest after the Br medium in autotrophic as well as mixotrophic conditions. The number of C. vulgaris cells in mixotrophic conditions was higher than S. armatus. The utilization of cheap growing media will lowering the production costs of algal biomass on a large scale.</p>

scholarly journals Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11217
Author(s):  
Chin Sze Yee ◽  
Victor Tosin Okomoda ◽  
Fakriah Hashim ◽  
Khor Waiho ◽  
Siti Rozaimah Sheikh Abdullah ◽  
...  
Keyword(s):  
Cell Density ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Axenic Culture ◽  
Cell Number ◽  
The Other ◽  
Biomass Growth ◽  
Microalgae Biomass ◽  
Insight Into

This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only Thalassiosira weissflogii, Chlamydomonas sp. and Chlorella vulgaris were propagated successfully in Conway medium. Hence, these species were selected for the experiment comparing microalgae axenic culture and co-culture with the floc-forming bacterium, Bacillus infantis. Results obtained showed that the co-culture had higher microalgae biomass compared to the axenic culture. A similar trend was also observed concerning the lipid content of the microalgae-bacterium co-cultures. The cell number of B. infantis co-cultured with T. weissflogii increased during the exponential stage until the sixth day, but the other microalgae species experienced a significant early reduction in cell density of the bacteria at the exponential stage. This study represents the first attempt at co-culturing microalgae with B. infantis, a floc-forming bacterium, and observed increased biomass growth and lipid accumulation compared to the axenic culture.

scholarly journals Cultivation of Chlorella vulgaris in Membrane-Treated Industrial Distillery Wastewater: Growth and Wastewater Treatment

2021 ◽  
Vol 9 ◽  
Author(s):  
Feng Li ◽  
David Kwame Amenorfenyo ◽  
Yulei Zhang ◽  
Ning Zhang ◽  
Changling Li ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Environmentally Friendly ◽  
Biomass Productivity ◽  
Industrial Effluents ◽  
Treatment Method ◽  
Pollution Potential ◽  
Microalgae Cultivation ◽  
Microalgae Biomass ◽  
Distillery Wastewater ◽  
Removal Efficiencies

The alcohol industry discharges large quantities of wastewater, which is hazardous and has a considerable pollution potential. Cultivating microalgae in wastewater is an alternative way of overcoming the current high cost of microalgae cultivation and an environmentally friendly treatment method for industrial effluents. The study analyzed the growth and biochemical composition of Chlorella vulgaris cultivated in membrane-treated distillery wastewater (MTDW) and nutrients removal efficiency. The results showed biomass productivity of 0.04 g L−1 d−1 for MTDW with the contents of content of protein, carbohydrate, and lipid at 49.6 ± 1.4%, 26.1 ± 0.6%, and 10.4 ± 1.8%, respectively. The removal efficiencies of TN, TP, and COD were 80, 94, and 72.24% in MTDW, respectively. In addition, removal efficiencies of 100, 85.37, and 42.86% for Ca2+, Mg2+, and Mo2− were achieved, respectively. The study added to our growing knowledge on the cultivation of Chlorella with wastewater, suggesting that it was feasible to cultivate Chlorella with MTDW and represented an economical and environmentally friendly strategy for microalgae biomass production and reuse of wastewater resources.

scholarly journals Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass

2020 ◽  
Vol 10 (6) ◽  
pp. 2156
Author(s):  
Adriana Machado ◽  
Hugo Pereira ◽  
Margarida Costa ◽  
Tamára Santos ◽  
Bernardo Carvalho ◽  
...  
Keyword(s):  
Growth Performance ◽  
Chlorella Vulgaris ◽  
Culture Medium ◽  
Bubble Column ◽  
Organic Medium ◽  
Organic Substrates ◽  
Microalgal Biomass ◽  
Organic C ◽  
Certified Organic ◽  
Organic Culture

Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Certified organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing a certified organic culture medium for the production of autotrophic Chlorella vulgaris biomass. A preliminary assay in 2 L bubble column photobioreactors was performed in order to screen different commercial organic substrates (OS) at a normalized concentration of N (2 mmol L−1). The highest growth performance was obtained using EcoMix4 and Bioscape which showed similar biomass concentrations compared to the synthetic culture medium (control). In order to meet the nutrient needs of Chlorella, both OS underwent elemental analyses to assess their nutrient composition. The laboratory findings allowed the development of a final organic culture medium using a proportion of Bioscape/EcoMix4 (1:1.2, m/m). This organic culture medium was later validated outdoors in 125 L flat panel and 10 m3 tubular flow through photobioreactors. The results obtained revealed that the developed organic medium led to similar microalgal growth performance and biochemical composition of produced biomass, as compared to the traditional synthetic medium. Overall, the formulated organic medium was effective for the autotrophic production of organic C. vulgaris biomass.

scholarly journals Membrane-Based Harvesting Processes for Microalgae and Their Valuable-Related Molecules: A Review

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 585
Author(s):  
Roberto Castro-Muñoz ◽  
Octavio García-Depraect
Keyword(s):  
Culture Medium ◽  
Forward Osmosis ◽  
Cost Effective ◽  
Research Community ◽  
Added Value ◽  
The Novel ◽  
The Third ◽  
Microalgae Biomass ◽  
Efficient Extraction ◽  
Membrane Technologies

The interest in microalgae production deals with its role as the third generation of feedstock to recover renewable energy. Today, there is a need to analyze the ultimate research and advances in recovering the microalgae biomass from the culture medium. Therefore, this review brings the current research developments (over the last three years) in the field of harvesting microalgae using membrane-based technologies (including microfiltration, ultrafiltration and forward osmosis). Initially, the principles of membrane technologies are given to outline the main parameters influencing their operation. The main strategies adopted by the research community for the harvesting of microalgae using membranes are subsequently addressed, paying particular attention to the novel achievements made for improving filtration performance and alleviating fouling. Moreover, this contribution also gives an overview of the advantages of applying membrane technologies for the efficient extraction of the high added-value compounds in microalgae cells, such as lipids, proteins and carbohydrates, which together with the production of renewable biofuels could boost the development of more sustainable and cost-effective microalgae biorefineries.

Sign in / Sign up

Export Citation Format

Share Document