scholarly journals Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass

2020 ◽  
Vol 10 (6) ◽  
pp. 2156
Author(s):  
Adriana Machado ◽  
Hugo Pereira ◽  
Margarida Costa ◽  
Tamára Santos ◽  
Bernardo Carvalho ◽  
...  
Keyword(s):  
Growth Performance ◽  
Chlorella Vulgaris ◽  
Culture Medium ◽  
Bubble Column ◽  
Organic Medium ◽  
Organic Substrates ◽  
Microalgal Biomass ◽  
Organic C ◽  
Certified Organic ◽  
Organic Culture

Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Certified organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing a certified organic culture medium for the production of autotrophic Chlorella vulgaris biomass. A preliminary assay in 2 L bubble column photobioreactors was performed in order to screen different commercial organic substrates (OS) at a normalized concentration of N (2 mmol L−1). The highest growth performance was obtained using EcoMix4 and Bioscape which showed similar biomass concentrations compared to the synthetic culture medium (control). In order to meet the nutrient needs of Chlorella, both OS underwent elemental analyses to assess their nutrient composition. The laboratory findings allowed the development of a final organic culture medium using a proportion of Bioscape/EcoMix4 (1:1.2, m/m). This organic culture medium was later validated outdoors in 125 L flat panel and 10 m3 tubular flow through photobioreactors. The results obtained revealed that the developed organic medium led to similar microalgal growth performance and biochemical composition of produced biomass, as compared to the traditional synthetic medium. Overall, the formulated organic medium was effective for the autotrophic production of organic C. vulgaris biomass.

scholarly journals Cultivation of Chlorella vulgaris in anaerobically digested gelatin industry wastewater

2020 ◽  
Author(s):  
G. C. Blanco ◽  
M. J. Stablein ◽  
G. Tommaso
Keyword(s):  
Chlorella Vulgaris ◽  
Culture Medium ◽  
High Efficiency ◽  
Bubble Column ◽  
Substrate Inhibition ◽  
Basal Medium ◽  
Biomass Productivity ◽  
Negative Control ◽  
Nitrogenous Compounds ◽  
Industry Wastewater

Abstract This work aimed to study the effect of using anaerobically digested gelatin industry wastewater as a culture medium for the cultivation of Chlorella vulgaris microalgae in bubble column photobioreactors (PBRs). Batch experiments were carried out to determine the growth kinetics by inoculating microalgae in wastewater prepared with different dilutions and supplemented with Bold's Basal Medium (BBM). From the values of the saturation constants (KS = 50.25 mgN-NH4+·L−1) and substrate inhibition (KI = 28.12 mgN-NH4+·L−1) obtained in the adjustment to the Andrews kinetic model (R2 = 0.817), the PBRs achieved specific maximum growth rates (μmax) of 0.343 d−1, biomass productivity of 0.141 g·L−1·d−1, lipid content of 12.45%, lipid productivity of 17.63 mg·L−1·d−1 and instantaneous ammoniacal nitrogen consumption rates of 20.06 and 14.22 mg·L−1·d−1. The addition of wastewater to the culture medium provided an increase in biomass productivity of 57.45% in relation to the negative control. The results obtained demonstrate the high efficiency of C. vulgaris in the removal of nitrogenous compounds and the potential of using anaerobically digested gelatin industry wastewater in the production of microalgae biomass.

scholarly journals Using ferric sulfate, sodium hydroxide, and chitosan to harvest marine microalgae Chlorella vulgaris and recycling the culture medium

2021 ◽  
Author(s):  
Jinling Cai ◽  
Yu Wang ◽  
Chenchen Feng ◽  
Xinzhi Zhang
Keyword(s):  
Sodium Hydroxide ◽  
Chlorella Vulgaris ◽  
Culture Medium ◽  
High Efficiency ◽  
Ferric Sulfate ◽  
Microalgal Biomass ◽  
Flocculation Efficiency ◽  
Marine Microalga ◽  
Dosage Requirement ◽  
Food And Feed

Abstract Microalgae are widely used in biofuels, medicine, food, and feed industries. However, harvesting microalgal biomass is a major difficulty that hinders their industrial application. In this study, three flocculants (ferric sulfate, sodium hydroxide, and chitosan) were used to harvest the marine microalga Chlorella vulgaris, and floc characteristics including flocculation efficiency, concentration factor, and flocs morphology were studied. The results showed that the tested flocculants can efficiently harvest C. vulgaris. The flocculation efficiencies of ferric sulfate (0.9 g/L), sodium hydroxide (0.6 g/L), and chitosan (30 mg/L) were 93.4% ± 0.8%, 96.5% ± 0.6%, and 98.8% ± 1.3% within 70, 100, and 12 min, respectively. The total carbohydrates, proteins, and lipids contents in C. vulgaris were not influenced by the test flocculants after harvesting. When compared with fresh f/2 medium, the recycled medium could also efficiently support C. vulgaris growth. Among the three flocculants tested, chitosan was ideal owing to its high efficiency, low dosage requirement, short harvesting time, and reutilization of culture medium.

scholarly journals Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Maria N. Metsoviti ◽  
George Papapolymerou ◽  
Ioannis T. Karapanagiotidis ◽  
Nikolaos Katsoulas
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Growth Rates ◽  
Solar Irradiance ◽  
White Led ◽  
Microalgal Biomass ◽  
Nm Range ◽  
Effect Of Light

In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

scholarly journals Study of Chlorella vulgaris sedimentation process

2018 ◽  
Vol 240 ◽  
pp. 05023 ◽  
Author(s):  
Agnieszka Patyna ◽  
Małgorzata Płaczek ◽  
Stanisław Witczak
Keyword(s):  
Chlorella Vulgaris ◽  
Dry Mass ◽  
Separation Process ◽  
Cultivation Condition ◽  
Microalgal Biomass ◽  
Algae Biomass ◽  
Algae Cultivation ◽  
Sedimentation Process ◽  
Microalgae Harvesting

The paper reports the results of Chlorella vulgaris sedimentation process including description of cultivation condition of microalgal biomass. The process of algae cultivation was carried out in photobioreactor comprising systems of carbon dioxide supply, mixing and artificial LED illumination. The growth of microalgae was determined alternatively in three ways by measuring the amount of dry mass over time, counting the cells and measurement of optical density by use of a spectrophotometer. Algae biomass with different concentration was subjected to the separation process by gravity. This led to the determination of the characteristic of sedimentation process for different concentrations and cell sizes. The experimental results indicate that sedimentation process offers a tool with a potential application for microalgae harvesting.

scholarly journals Flow behavior analysis of Chlorella Vulgaris microalgal biomass

Heliyon ◽  
2019 ◽  
Vol 5 (6) ◽  
pp. e01845
Author(s):  
Suresh Kumar Yatirajula ◽  
Anuj Shrivastava ◽  
Vinod Kumar Saxena ◽  
Jagadeeshwar Kodavaty
Keyword(s):  
Behavior Analysis ◽  
Chlorella Vulgaris ◽  
Flow Behavior ◽  
Microalgal Biomass

Comparison of three carbon determination methods on naturally occurring substrates and the implication for the quantification of 'soil carbon'

Soil Research ◽  
2011 ◽  
Vol 49 (1) ◽  
pp. 27 ◽  
Author(s):  
M. K. Conyers ◽  
G. J. Poile ◽  
A. A. Oates ◽  
D. Waters ◽  
K. Y. Chan
Keyword(s):  
Soil Carbon ◽  
Annual Variation ◽  
Organic Substrates ◽  
Test Procedures ◽  
Organic C ◽  
Soil C ◽  
Long Term Trends ◽  
Carbon Determination ◽  
Almost All

Accounting for carbon (C) in soil will require a degree of precision sufficient to permit an assessment of any trend through time. Soil can contain many chemically and physically diverse forms of organic and inorganic carbon, some of which might not meet certain definitions of ‘soil carbon’. In an attempt to assess how measurements of these diverse forms of C might vary with analytical method, we measured the C concentration of 26 substrates by three methods commonly used for soil C (Walkley–Black, Heanes, and Leco). The Heanes and Leco methods were essentially equivalent in their capture of organic C, but the Leco method captured almost all of the inorganic C (carbonates, graphite). The Heanes and Walkley–Black methods did not measure carbonates but did measure 92% and 9%, respectively, of the C in graphite. All three of the common soil test procedures measured some proportion of the charcoal and of the other burnt materials. The proportion of common organic substrates (not the carbonates, graphite, or soil) that was C by weight ranged from ~10% to 90% based on the Heanes and Leco data. The proportion of the organic fraction of those same substrates, as measured by loss-on-ignition, that was C by weight ranged from 42% to 100%. The relationship between Walkley–Black C and total C (by Heanes and Leco) showed that Walkley–Black C was a variable proportion of total C for the 26 substrates. Finally, the well-known, apparent artefact in the Cr-acid methods was investigated: dichromate digestion should contain at least 7–10 mg C in the sample or over-recovery of C might be reported. Our observation that common soil C procedures readily measure C in plant roots and shoots, and in burnt stubble, means that there will likely be intra-annual variation in soil C, because avoidance of these fresh residues is difficult. Such apparent intra-annual variation in soil C will make the detection of long-term trends problematic.

Comparison of conventional and organic apple production systems during three years of conversion to organic management in coastal California

1998 ◽  
Vol 13 (4) ◽  
pp. 162-180 ◽  
Author(s):  
Sean L. Swezey ◽  
Matthew R. Werner ◽  
Marc Buchanan ◽  
Jan Allison
Keyword(s):  
Production System ◽  
Production Systems ◽  
Codling Moth ◽  
Organic Production ◽  
Leaf Damage ◽  
Organic Management ◽  
Organic C ◽  
Apple Production ◽  
Wood Bark ◽  
Certified Organic

AbstractConventional and organic semidwarf Granny Smith apple production systems were compared during three years of conversion to certified organic management. Because of differences in fruit load with hand thinning compared with chemical thinning, apple tonnage was higher in the organic production system (OPS) in 1989 and 1991. The organic system was higher than the conventional system in number and weight of fruit per tree, but smaller in average fruit size. Using grower-receivedfarmgate premiums of 38% (1990) and 33% (1991) for unsorted, certified organic apples, comparative cost accounting showed greater net return per hectare for the OPS. The OPS required higher material and labor inputs in all years.Greater terminal growth in the conventional production system (CPS) in 1991 was the only significant difference in growth indicators between systems. N was generally higher in leaf and new wood bark tissues in the CPS. P was generally higher in the leaf and new wood bark tissues in the OPS. No decline in yield was associated with increased weed biomass in the OPS. There was no difference in fruit damage caused by codling moth between production system treatments (codling moth granulosis virus and pheromone-based mating disruption vs. synthetic insecticide). In 1991, secondary lepidopterous pests (apple leafroller and orange tortrix) caused greater fruit scarring in the CPS. In all years, tentiform leafminers caused greater leaf damage in the CPS. Apple leafhopper density and leaf damage were greater in the OPS in 1990 and 1991.Soil nutrient levels showed few significant changes during conversion to organic management. Soil bulk density and water holding capacity were useful indicators of changes in soil physical characteristics. Potentially mineralizable nitrogen andmicrobial biomass-C were more sensitive indicators of system change than total N or organic C. Two soil biological ratios, the respiratory ratio and biomass-C/total organic-C, were similar in the two production systems. Earthworm biomass and abundance increased in the OPS in the third year. The introduction ofLumbricus terrestrisinto the OPS greatly increased litter incorporation rates.

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