Performance and microbial protein expression during anaerobic treatment of alkali-decrement wastewater using a strengthened circulation anaerobic reactor

2019 ◽  
Vol 273 ◽  
pp. 40-48 ◽  
Author(s):  
Bo Yang ◽  
Qing Wang ◽  
Jinshao Ye ◽  
Hui Xu ◽  
Yanbiao Liu ◽  
...  
Keyword(s):  
Protein Expression ◽  
Anaerobic Treatment ◽  
Microbial Protein ◽  
Anaerobic Reactor

scholarly journals ANÁLISE DA EFICIÊNCIA DO TRATAMENTO ANAERÓBIO DA MANIPUEIRA

2021 ◽  
Vol 12 (4) ◽  
pp. 30-37
Author(s):  
Danielle Florindo Pereira ◽  
Debora Yukie Pereira Ishida ◽  
Júlio Kazuya Itimura Omori ◽  
Alexandre Teixeira de Souza
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Anaerobic Treatment ◽  
Domestic Sewage ◽  
Effluent Treatment ◽  
Total Volatile ◽  
Anaerobic Reactor ◽  
Total Solids ◽  
Volatile Solids

In the processes of industrialization of cassava flour (Minihot esculenta), there is the generation of an effluent called manipueira, which requires treatment before being released into the water body. The objective of this work was to evaluate the efficiency of effluent degradation by an anaerobic reactor, using as substrate, manipueira of a flour producing industry, and, as inoculum, two distinct sludges, one from an anaerobic reactor of ascending flow (RAFA) of domestic sewage treatment plant, and another from the activated sludge system of effluent treatment of a grain producing industry. The efficiency of the process was evaluated by the physicochemical parameters: COD, BOD, total solids (TS), total volatile solids (STV), fixed total solids (STF), pH, turbidity and cyanide. Throughout the treatment there was a significant removal of Cyanide in the effluent, that is, one of the major problems of the effluent can be solved effectively via anaerobic treatment.

Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

1998 ◽  
Vol 38 (12) ◽  
pp. 123-126 ◽  
Author(s):  
C. Burtscher ◽  
P. A. Fall ◽  
O. Christ ◽  
P. A. Wilderer ◽  
S. Wuertz
Keyword(s):  
Pathogenic Bacteria ◽  
Organic Waste ◽  
Waste Treatment ◽  
Anaerobic Treatment ◽  
Direct Extraction ◽  
Two Stage ◽  
Anaerobic Reactor ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Pcr Technology

The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.

Field experience with different systems for biomass accumulation in anaerobic reactor technology

1994 ◽  
Vol 30 (12) ◽  
pp. 181-191 ◽  
Author(s):  
D. Defour ◽  
D. Derycke ◽  
J. Liessens ◽  
P. Pipyn
Keyword(s):  
Industrial Effluents ◽  
Biomass Accumulation ◽  
Anaerobic Treatment ◽  
Uasb Reactor ◽  
Anaerobic Reactor ◽  
Reactor Technology ◽  
Accumulation System ◽  
Three Phase ◽  
Phase Separator ◽  
Hybrid Reactors

Thanks to undeniable advantages as recovery of energy and a low sludge production, anaerobic wastewater treatment has received recognition in the last decade. However due to the slow methanogenic growth, inreactor biomass accumulation is essential to maintain high loading rates. Indeed wash-out of biomass is one of the main problems encountered in the anaerobic treatment of industrial effluents. A broad scoped overview of the different reactor technologies is given each with its proper biomass accumulation system. All of them are founded on two basic mechanisms for biomass accumulation: separation and retention. Settling (Anaerobic Contact reactor), ultrafiltration and flotation (FlotametP) are the techniques used to separate externally the biomass from the effluent after which the biomass can be recirculated to the anaerobic reactor. Concentration of biomass can also be achieved by attachment to a mobile (Fluidized Bed reactor) or a static carrier with possible entrapment in its macroporous structure (Anaerobic Filter reactor). The UASB-reactor incorporates retention as well as separation. By stimulating granular growth, biomass is accumulated in the lower part of the reactor. A three phase separator at the top of the reactor contributes to the final clarification of the effluent. Hybrid reactors eventually combine different biomass accumulation mechanisms: granulation, external biomass separation (Upflow Anaerobic Contact reactor) and fixation (Upflow Anaerobic Contact Filter reactor).

Anaerobic Treatment of the Centrifuged Solid Fraction of Piggery Wastewater in an Inclined Plug Flow Reactor

1993 ◽  
Vol 28 (2) ◽  
pp. 107-114 ◽  
Author(s):  
J. Górecki ◽  
G. Bortone ◽  
A. Tilche
Keyword(s):  
Flow Reactor ◽  
Plug Flow ◽  
Anaerobic Treatment ◽  
Organic Matter Degradation ◽  
Piggery Wastewater ◽  
Feed Material ◽  
Anaerobic Reactor ◽  
Loading Rates ◽  
Low Phosphorus ◽  
Phosphorus Levels

This paper reports the experimental results of a 6 months run on a bench scale inclined tubular anaerobic reactor treating centrifuged piggery sludge at 12-18% TS. The particular reactor design was demonstrated to be appropriate for handling this very dense feed material. Reactor performances were very satisfactory: with increasing loading rates up to 10 g COD.1−1.d−1 (corresponding to 12 d HRT the organic matter degradation was always constant around 60%. Methane yield was calculated around 321 CH4/l raw feed. The effluent was well dewaterable and showed low phosphorus levels in the liquid phase. A curious phenomenon of granulation is also discussed.

Effects of calcium concentration on up-flow multistage anaerobic reactor performance in treating bagasse spraying wastewater

BioResources ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 4254-4269
Author(s):  
Jinghong Zhou ◽  
Xiaona Shang ◽  
Zhiwei Wang ◽  
Cancan Zhu ◽  
Shuangfei Wang
Keyword(s):  
Anaerobic Digestion ◽  
Loading Rate ◽  
Biogas Production ◽  
Scale Up ◽  
Anaerobic Treatment ◽  
Cod Removal ◽  
Microbial Community Analysis ◽  
Organic Loading Rate ◽  
Reactor Performance ◽  
Anaerobic Reactor

Bagasse spraying wastewater (BSW) is a source of organic pollutants during bagasse processing. In this study, the feasibility of anaerobic treatment of BSW under different calcium concentrations (60 to 2400 mg/L) was studied. The experiment was performed in a lab-scale up-flow multistage anaerobic reactor (UMAR) inoculated with granular sludge, and operated for 160 days at a constant organic loading rate of 6 kg COD/(m3·d). Treatment of BSW with 60 to 800 mg Ca2+/L resulted in 80.7 to 82.7% of COD removal, 161 to 232.7 mg COD/L of volatile fatty acid (VFA) yield, 0.56 to 0.79 m3/(kgCOD·d) of biogas production rate, and 2.4 to 2.66 m3/(m3·d) of volume loading rate (VLR). The pH remained within the optimal range for anaerobic digestion (adjust to pH = 6.8 to 7.0). The VFAs were composed of 77 to 85% acetic acid, 8.4 to 13.2% butyric acid, and 6.6 to 9.6% propionic acid. At higher influent calcium concentrations (> 800 mg/L), the hydrolysis process appeared to be inhibited, affecting the anaerobic digestion performance of the reactor. In particular, the COD removal efficiency decreased to 55.5%, and the VFA content in the effluent significantly increased due to the lower pH. Microbial community analysis showed that at the end of anaerobic digestion, the Syntrophobacter disappeared, and Clostridium and Anerolineaceae were the main genus and family, respectively. Overall, the results indicated that low calcium (< 300 mg/L) had a positive effect on the UMAR performance.

scholarly journals Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

2020 ◽  
Vol 8 (3) ◽  
pp. 379
Author(s):  
Sheng Pan ◽  
Meredith A. J. Hullar ◽  
Lisa A. Lai ◽  
Hong Peng ◽  
Damon H. May ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dietary Patterns ◽  
Gut Microbiome ◽  
Dietary Intervention ◽  
Glycemic Load ◽  
Human Microbiome ◽  
Human Microbiome Project ◽  
Microbial Metabolism ◽  
Microbial Protein ◽  
Bacterial Enzymes

Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR) <10%, were used to compare the Gene Ontology (GO) biological processes and bacterial enzymes between the two interventions. Using shotgun proteomics, more than 53,000 unique peptides were identified including microbial (89%) and human peptides (11%). Forty-eight bacterial enzymes were statistically different between the diets, including those implicated in SCFA production and degradation of fatty acids. Enzymes associated with degradation of human mucin were significantly enriched in the RG diet. These results illustrate that the metaproteomic approach is a valuable tool to study the microbial metabolism of diets that may influence host health.

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