Cultivation of Chlorella vulgaris on dairy waste using vision imaging for biomass growth monitoring

2021 ◽  
Vol 341 ◽  
pp. 125892
Author(s):  
Angela Paul Peter ◽  
Kit Wayne Chew ◽  
Apurav Krishna Koyande ◽  
Sia Yuk-Heng ◽  
Huong Yong Ting ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Growth Monitoring ◽  
Biomass Growth ◽  
Dairy Waste

scholarly journals Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source

2012 ◽  
Vol 118 ◽  
pp. 61-66 ◽  
Author(s):  
Ana P. Abreu ◽  
Bruno Fernandes ◽  
António A. Vicente ◽  
José Teixeira ◽  
Giuliano Dragone
Keyword(s):  
Carbon Source ◽  
Organic Carbon ◽  
Chlorella Vulgaris ◽  
Mixotrophic Cultivation ◽  
Dairy Waste ◽  
Organic Carbon Source

scholarly journals Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11217
Author(s):  
Chin Sze Yee ◽  
Victor Tosin Okomoda ◽  
Fakriah Hashim ◽  
Khor Waiho ◽  
Siti Rozaimah Sheikh Abdullah ◽  
...  
Keyword(s):  
Cell Density ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Axenic Culture ◽  
Cell Number ◽  
The Other ◽  
Biomass Growth ◽  
Microalgae Biomass ◽  
Insight Into

This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only Thalassiosira weissflogii, Chlamydomonas sp. and Chlorella vulgaris were propagated successfully in Conway medium. Hence, these species were selected for the experiment comparing microalgae axenic culture and co-culture with the floc-forming bacterium, Bacillus infantis. Results obtained showed that the co-culture had higher microalgae biomass compared to the axenic culture. A similar trend was also observed concerning the lipid content of the microalgae-bacterium co-cultures. The cell number of B. infantis co-cultured with T. weissflogii increased during the exponential stage until the sixth day, but the other microalgae species experienced a significant early reduction in cell density of the bacteria at the exponential stage. This study represents the first attempt at co-culturing microalgae with B. infantis, a floc-forming bacterium, and observed increased biomass growth and lipid accumulation compared to the axenic culture.

Survey on nitrogen removal and membrane filtration characteristics of Chlorella vulgaris Beij. on treating domestic type wastewaters

2013 ◽  
Vol 68 (3) ◽  
pp. 695-704 ◽  
Author(s):  
Yu-Hsuan Wang ◽  
Chuen-Mei Wu ◽  
Wan-Lin Wu ◽  
Ching-Ping Chu ◽  
Yu-Jen Chung ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Chlorella Vulgaris ◽  
Membrane Filtration ◽  
Domestic Wastewater ◽  
Nitrifying Bacteria ◽  
Biomass Growth ◽  
Filtration Cycle ◽  
The Mean ◽  
Cell Residence Time ◽  
Uf Membranes

The main objective of this study is to evaluate the nitrogen assimilation and filtration characteristics of Chlorella vulgaris Beij. when treating domestic wastewaters. Chlorella could assimilate organic nitrogen, ammonia and nitrate in wastewater, and the mean cell residence time (MCRT) to achieve the maximum biomass content in a bioreactor was different for each individual nitrogen source used. The experimental results showed that using nitrate as the only nitrogen source was the most favorable for biomass growth. With ammonia and nitrate coexisting in the aquatic phase, Chlorella possibly utilized ammonia first, and this was unfavorable to subsequent biomass growth. Nitrifying bacteria in wastewaters significantly affected Chlorella growth as they possibly competed with Chlorella in assimilating ammonia and nitrate in domestic wastewater. In a submerged ultrafiltration (UF) membrane module, with an initial concentration of 850 mg/L of Chlorella, the optimized flux was 0.02 m3/(m2·h), and the filtration cycle was 30 min. A ‘dual membrane bioreactor (MBR)’ configuration using UF membranes for Chlorella incubation was proposed. MBR1 provides an environment with long MCRT for efficient nitrification. The converted nitrate is assimilated by Chlorella in MBR2 to sustain its growth. UF permeate from MBR1 is bacteria-free and does not affect the growth of Chlorella in MBR2. MCRT of Chlorella growth is controlled by the UF membrane of MBR2, providing the flexibility to adjust variations of nitrogen composition in the wastewater.

scholarly journals Chlorella Vulgaris Surface-Mount Photobioreactor with Vision-Based Growth Signature Prediction Optimized by Electromagnetism-Like Mechanism

2020 ◽  
Vol 9 (4) ◽  
pp. 378-387
Keyword(s):  
Light Intensity ◽  
Water Temperature ◽  
Surface Area ◽  
Chlorella Vulgaris ◽  
Intelligent System ◽  
Energy Resource ◽  
Controlled Environment ◽  
Growth Monitoring ◽  
Surface Mount ◽  
Microalgae Growth

Industrial waste disrupts the natural production of microalgae cultures. Cultivation of microalgae in a controlled environment highly results to biomass with lower contamination necessary as high-valued economic product. In response to the emerging challenges of sustainable energy production, the integration of computational intelligence and biosystems engineering is considered as an open research area. In this study, Chlorella vulgaris microalgae were cultivated in BG-11 growth medium on three customized surface-mount light bioreactors that are equipped with digital camera for growth monitoring in terms of accumulated biomass surface area and color reflectance intensity via IoT. Feature-based machine learning models predicted microalgae growth area in terms of water temperature, pH level and turbidity, and light intensity. Microalgae cultures were exposed to combinations of white artificial light source of 2000 ± 1000 lux and water temperature of 27 ± 5°C using Peltier plate to discriminate biomass growth within a 30-day cultivation period. A total of nine environmental conditions were employed to clearly discriminate the impacts of environmental stressors to microalgae growth. Combined neighborhood component analysis and ReliefF was used to select high impact color features of C, Ye, M, H, and S with biomass area. Electromagnetism-like mechanism optimized-RBNN bested RNN and generalized processing regression with R2 of 0.985 and RMSE of 6.262. There is also considerable growth in biomass surface area for certain combinations of light intensity and water temperature (2125 ± 625 lux and 28.75 ± 3.25°C), and turbidity and water pH concentrations (3.85 ± 0.15 NTU and 8.025 ± 0.775). However, the photobioreactor with 27°C and 2000 lux exposure is considered having the exact optimum controlled environment condition in cultivating Chlorella vulgaris based on the generated growth in biomass surface area of 38.314%. This developed intelligent system is scalable for seamless microalgae production of any strands for renewable energy resource.

Effect of Synthetic Wastewater by Electrochemical Pretreatment on Chlorella vulgaris Growth and Nutrients Removal

2013 ◽  
Vol 666 ◽  
pp. 33-42 ◽  
Author(s):  
Meng Zi Wang ◽  
Zhi Wei Zhu ◽  
Wei Cao ◽  
Hong De Zhou ◽  
Yu Wu ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Biological Treatment ◽  
Dry Weight ◽  
Synthetic Wastewater ◽  
Biomass Growth ◽  
Nutrients Removal ◽  
Microalgae Cultivation ◽  
Electrochemical Processing ◽  
Electrochemical Pretreatment ◽  
Organic Wastewater

Electrochemical processing combined with the system of microalgaeChlorella vulgariswas used to treat the synthetic organic wastewater in this paper. The effect of wastewater concentration on the biomass growth and nutrients removal was investigated. Three levels of the wastewater concentrations were ranked as Low, Mid and High, respectively. After 2 h of electrolysis pretreatment and 10 d of microalgae cultivation, TOC, NH4-N, and TP concentrations in the group Low were reduced by 83.7%, 99.3% and 95.0%, respectively. TheChlorella vulgarisin the groups Mid and High without electrolysis pretreatment did not survive longer than 24 h, whereas it grown well in the wastewater pretreated by electrolysis. The dry weight (DW) ofChlorella vulgarisin the group Low with electrolysis pretreatment was increased from 0.048 g/l to 1.087 g/l by 10 d cultivation. Results indicate that electrolysis pretreatment for wastewater can provide appropriate conditions for the subsequent biological treatment and efficiently promote the biomass growth ofChlorella vulgaris.

Biomass growth monitoring using pressure drop in a cocurrent biofilter

1998 ◽  
Vol 60 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Marc Deront ◽  
Falilou M. Samb ◽  
Nevenka Adler ◽  
Paul Péringer
Keyword(s):  
Pressure Drop ◽  
Growth Monitoring ◽  
Biomass Growth

Feasible design for electricity generation from Chlorella vulgaris using convenient photosynthetic conditions

BIOCELL ◽  
2018 ◽  
Vol 42 (1) ◽  
pp. 7-11 ◽  
Author(s):  
M. Moustafa ◽  
T. Taha ◽  
M. Elnouby ◽  
M.A. Abu-Saied Aied ◽  
A. Shati ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Electricity Generation

Chlorella vulgaris Beijer. as biosorbent: biosorbtion of gold and physiological principles of regeneration

2000 ◽  
Vol 2 (3) ◽  
pp. 1-10
Author(s):  
T. G. Gruzina ◽  
L. G. Stepura ◽  
M. N. Balakina ◽  
Z. R. Ulberg
Keyword(s):  
Chlorella Vulgaris

Investigation of culture conditions by Response Surface Methodology and kinetic modeling for exopolysaccharide production by Klebsiella oxytoca ICCF 419 strain, using lactose as substrate

2020 ◽  
Vol 25 (6) ◽  
pp. 2033-2044
Author(s):  
CATERINA TOMULESCU ◽  
◽  
MIŞU MOSCOVICI ◽  
ROXANA MĂDĂLINA STOICA ◽  
GABRIEL BUJOR ALBU ◽  
...  
Keyword(s):  
Citric Acid ◽  
Klebsiella Oxytoca ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Biomass Growth ◽  
Initial Value ◽  
Substrate Consumption ◽  
Polysaccharide Production ◽  
Crude Polysaccharide ◽  
Central Composite

The objective of this work was to optimize the bioprocess parameters, using Klebsiella oxytoca ICCF 419 to obtain an exopolysaccharide based on lactose as substrate. A kinetic study was employed and Logistic and Gompertz models were applied to describe the polysaccharide production, in relation with biomass growth and substrate consumption. The RSM methodology based on Central Composite Rotatable Design was used to evaluate and optimize the effect of lactose, corn extract, KH2PO4 and citric acid concentrations as independent variables on the polysaccharide production, biomass growth and substrate consumption as the response functions. The interaction effects and optimal parameters were obtained using Design Expert Software (version 9.0.6.2). The significance of the variables and their interactions was tested by means of ANOVA analysis with a 99% confidence level. The optimum culture conditions were determined and the model prediction was compared with experimental results. At an initial value of 23.45 for the C/N in the fermentation medium, the strain produces 17.41 g/L of crude polysaccharide and 2.53 g/L dry biomass. The EPS production was significantly influenced by lactose, corn extract and KH2PO4, while the citric acid had no influence. The biomass growth was influenced by the corn extract, KH2PO4 and citric acid.

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