Bionanohybrid composed of metalloprotein/DNA/MoS2/peptides to control the intracellular redox states of living cells and its applicability as a cell-based biomemory device

2021 ◽  
pp. 113725
Author(s):  
Jinho Yoon ◽  
Minkyu Shin ◽  
Dongyeon Kim ◽  
Joungpyo Lim ◽  
Hyun-Woong Kim ◽  
...  
Keyword(s):  
Living Cells ◽  
Redox States ◽  
A Cell ◽  
Intracellular Redox

scholarly journals Methods and applications of label-free cell-based systems

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Joachim Wiest
Keyword(s):  
Environmental Monitoring ◽  
Morphological Changes ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Controlled System ◽  
Chip Technology ◽  
Cell Monitoring ◽  
A Cell ◽  
On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

scholarly journals A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qixin Chen ◽  
Hongbao Fang ◽  
Xintian Shao ◽  
Zhiqi Tian ◽  
Shanshan Geng ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Molecular Probe ◽  
Live Cells ◽  
Blue Fluorescence ◽  
Red Fluorescence ◽  
Dynamic Tracking ◽  
A Cell

AbstractMitochondria–lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red–blue fluorescence intensity indicates the progress of mitochondria–lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria–lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria–lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria–lysosome interactions in living cells.

scholarly journals Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 399 ◽  
Author(s):  
Shinako Bansai ◽  
Takashi Morikura ◽  
Hiroaki Onoe ◽  
Shogo Miyata
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscles ◽  
Malignant Tumors ◽  
Three Dimensional ◽  
Living Cells ◽  
Cyclic Stretch ◽  
Core Shell ◽  
Custom Made ◽  
A Cell

Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

scholarly journals Long-distance electron transport in individual, living cable bacteria

2018 ◽  
Vol 115 (22) ◽  
pp. 5786-5791 ◽  
Author(s):  
Jesper T. Bjerg ◽  
Henricus T. S. Boschker ◽  
Steffen Larsen ◽  
David Berry ◽  
Markus Schmid ◽  
...  
Keyword(s):  
Electron Transport ◽  
Laser Cutting ◽  
Direct Evidence ◽  
Living Cells ◽  
Long Distance ◽  
Bacteria Transport ◽  
Redox States ◽  
Photosynthetic Organisms ◽  
Electron Sink ◽  
Rapid Shift

Electron transport within living cells is essential for energy conservation in all respiring and photosynthetic organisms. While a few bacteria transport electrons over micrometer distances to their surroundings, filaments of cable bacteria are hypothesized to conduct electric currents over centimeter distances. We used resonance Raman microscopy to analyze cytochrome redox states in living cable bacteria. Cable-bacteria filaments were placed in microscope chambers with sulfide as electron source and oxygen as electron sink at opposite ends. Along individual filaments a gradient in cytochrome redox potential was detected, which immediately broke down upon removal of oxygen or laser cutting of the filaments. Without access to oxygen, a rapid shift toward more reduced cytochromes was observed, as electrons were no longer drained from the filament but accumulated in the cellular cytochromes. These results provide direct evidence for long-distance electron transport in living multicellular bacteria.

Considerations of the importance of redox state for reactive nitrogen species action

2019 ◽  
Vol 70 (17) ◽  
pp. 4323-4331 ◽  
Author(s):  
John T Hancock
Keyword(s):  
Reactive Nitrogen Species ◽  
Redox State ◽  
Physiological Responses ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Signalling Molecules ◽  
Redox Environment ◽  
A Cell ◽  
Redox Poise ◽  
Intracellular Redox

Abstract Nitric oxide (NO) and other reactive nitrogen species (RNS) are immensely important signalling molecules in plants, being involved in a range of physiological responses. However, the exact way in which NO fits into signal transduction pathways is not always easy to understand. Here, some of the issues that should be considered are discussed. This includes how NO may interact directly with other reactive signals, such as reactive oxygen and sulfur species, how NO metabolism is almost certainly compartmentalized, that threshold levels of RNS may need to be reached to have effects, and how the intracellular redox environment may impact on NO signalling. Until better tools are available to understand how NO is generated in cells, where it accumulates, and to what levels it reaches, it will be hard to get a full understanding of NO signalling. The interaction of RNS metabolism with the intracellular redox environment needs further investigation. A changing redox poise will impact on whether RNS species can thrive in or around cells. Such mechanisms will determine whether specific RNS can indeed control the responses needed by a cell.

scholarly journals Visualizing Gene Expression in Real-Time

2005 ◽  
Vol 13 (3) ◽  
pp. 3-7
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Real Time ◽  
Temporal Resolution ◽  
Protein Sequence ◽  
Living Cells ◽  
Entire Process ◽  
Gene Chips ◽  
A Cell ◽  
Dna Template

Gene expression has been visualized for a few decades, but in static forms such as blots and gene chips. Susan Janicki, Toshiro Tsukamoto, Sim one Salghetti, William Tansey, Ravi Sachidanandam, Kannanganattu Prasanth, Thomas Ried, Yaron Shav-Tal, Edouard Bertrand, Robert Singer, and David Spector have recently designed a cell line in which gene expression can be observed with stunningly accurate spatial and temporal resolution. Gene expression is a cascade of events beginning with transcription of RNA from the DNA template and ending with translation into a protein sequence. Janicki et al. were able to visualize the entire process at the levels of DNA, RNA, and proteins in living cells!

scholarly journals Simultaneous intracellular redox potential and pH measurements in live cells using SERS nanosensors

The Analyst ◽  
2015 ◽  
Vol 140 (7) ◽  
pp. 2330-2335 ◽  
Author(s):  
L. E. Jamieson ◽  
A. Jaworska ◽  
J. Jiang ◽  
M. Baranska ◽  
D. J. Harrison ◽  
...  
Keyword(s):  
Redox Potential ◽  
Single Cell ◽  
Live Cells ◽  
Ph Measurements ◽  
Cell Basis ◽  
Redox Responsive ◽  
Cell Measurement ◽  
A Cell ◽  
Intracellular Redox

In this paper we have presented the first example of multiplexing pH and redox responsive SERS nanosensors for intracellular live single cell measurement on a cell by cell basis.

The intracellular redox states of HeLa cells under conditions suitable for radiobiological studies

1972 ◽  
Vol 45 (529) ◽  
pp. 59-65
Author(s):  
B. Chance ◽  
V. Legallais ◽  
N. Graham ◽  
N. Oshino
Keyword(s):  
Hela Cells ◽  
Redox States ◽  
Intracellular Redox

scholarly journals Printed Life

2012 ◽  
Vol 134 (01) ◽  
pp. 44-47 ◽  
Author(s):  
Jean Thilmany
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Body Part ◽  
Living Cells ◽  
Layer By Layer ◽  
Collagen Scaffolds ◽  
Three Dimensional Printing ◽  
A Cell ◽  
Dimensional Object ◽  
Dimensional Printing

This article discusses the advancement in bioprinting technology that would enable three-dimensional printing of living organs for transplant. Today, artificial or replacement tissue is commonly grown on collagen scaffolds that contain biological starter cells. The goal here is the growing of a biocompatible piece of tissue to repair or replace a patient’s own damaged body part, such as bone, cartilage, blood vessels, or skin. In future, bioprinting technology will allow making living organs for transplant. The method is much the same as additive manufacturing, in which a printer deposits a material, layer by layer, until a three-dimensional object is made. For bioprinting, the material used is likely to be living cells taken directly from the patient’s body and infused into an ink or gel to keep them alive. After printing, the material is incubated in a cell culture that mimics human body conditions until it fuses or becomes otherwise usable for implant.

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