Modification of mesoporous structure of silver-doped bioactive glass with antibacterial properties for bone tissue applications

2021 ◽  
Author(s):  
Sara Mortazavi ◽  
Mansour Rahsepar ◽  
Saeid Hosseinzadeh
Keyword(s):  
Bioactive Glass ◽  
Bone Tissue ◽  
Antibacterial Properties ◽  
Mesoporous Structure

scholarly journals Manuka Honey and Zein Coatings Impart Bioactive Glass Bone Tissue Scaffolds Antibacterial Properties and Superior Mechanical Properties

2021 ◽  
Vol 7 ◽  
Author(s):  
Marcela Arango-Ospina ◽  
Kristina Lasch ◽  
Julius Weidinger ◽  
Aldo R. Boccaccini
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Bone Tissue ◽  
Wound Care ◽  
Antibacterial Properties ◽  
Manuka Honey ◽  
Corn Protein ◽  
Effective Combination ◽  
Colony Forming Units ◽  
Superior Mechanical Properties

The combination of traditional herbal medicine (phytotherapeutic agents) with bioactive glasses is a promising strategy to generate advanced scaffolds for bone tissue engineering (BTE). An old remedy used for wound care since ancient times is honey. The antioxidant, antimicrobial and antibacterial properties of Manuka honey, in particular, make it an attractive substance for application in BTE scaffolds to prevent infections and biofilm formation. In this study 45S5 bioactive glass-based scaffolds produced via the foam replica technique were coated with corn protein zein and Manuka honey with two purposes: to improve the mechanical properties of the brittle scaffolds and to impart antibacterial properties. The morphology and chemical composition of the coated scaffolds were characterized with scanning electron microscopy and Fourier transform infrared spectroscopy, respectively, demonstrating the presence of Manuka honey in the coating. The release of the honey was quantified via ultraviolet-visible spectrophotometry; moreover, the antibacterial activity against Staphylococcus aureus was evaluated via colony-forming units counting, reduction of Alamar blue and turbidity measurements. Our findings suggest the effective combination of Manuka honey and bioactive glass, adding one more system to the novel family of bioactive glass scaffolds functionalized with phytotherapeutic agents.

scholarly journals Evaluation of antibacterial properties of hydroxyapatite/bioactive glass and fluorapatite/bioactive glass nanocomposite foams as a cellular scaffold of bone tissue

2018 ◽  
Vol 10 (03) ◽  
pp. 265-270 ◽  
Author(s):  
Seyedali Seyedmajidi ◽  
Ramazan Rajabnia ◽  
Maryam Seyedmajidi
Keyword(s):  
Staphylococcus Aureus ◽  
Bioactive Glass ◽  
Bone Tissue ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Agar Medium ◽  
Antibacterial Properties ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Nanocomposite Foams

ABSTRACT AIMS AND OBJECTIVES: Infection is a serious problem for patients after implantation surgery, which is difficult to treat with antibiotic therapy. The present study was developed to evaluate and compare the antibacterial properties of hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/ BG) nanocomposite foams as a cellular scaffold for use in bone defects by two macrodilution and disk diffusion methods. MATERIALS AND METHODS: Staphylococcus aureus, Enterococcus faecalis, and Streptococcus mutans were cultured in brain heart infusion broth medium with nanocomposite powder for 5 days, and their bioactivity levels were evaluated by daily culturing on solid agar medium plates. To carry out the disk diffusion test, a disc form of nanocomposite foams was used on agar medium with 48 h incubation. Results: None of two nanocomposites even at their highest concentration (200 mg/mL) did not prevent the growth of two Staphylococcus aureus and Enterococcus faecalis microorganisms. However, HA/BG nanocomposite on the 3rd day at a concentration of 200 mg/mL and on 4th and 5th day at a concentration of 100 mg/mL and FA/BG nanocomposite on the 4th day at a concentration of 100 mg/mL and on the 5th day at a concentration of 50 mg/mL could be able to kill Streptococcus mutans microorganism. In the disc diffusion test, none of the nanocomposites could create a nongrowth zone. Both tested biomaterials showed increased antibacterial properties over time and concentration increase. Conclusions: HA/BG and FA/BG nanocomposites, due to their biocompatibility and antimicrobial properties, are good choices for implantation instead of damaged bone tissue in tissue engineering.

scholarly journals Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 34
Author(s):  
Shaher Bano ◽  
Memoona Akhtar ◽  
Muhammad Yasir ◽  
Muhammad Salman Maqbool ◽  
Akbar Niaz ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Composite Coatings ◽  
Antibacterial Properties ◽  
Bone Diseases ◽  
Osteogenic Potential ◽  
Diffusion Method ◽  
Charge Composition ◽  
In Vitro Bioactivity ◽  
Mesoporous Bioactive Glass

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

scholarly journals Mesoporous Properties of Bioactive Glass Synthesized by Spray Pyrolysis with Various Polyethylene Glycol and Acid Additions

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 618
Author(s):  
Tzu-Yu Peng ◽  
Pei-Yun Tsai ◽  
May-Show Chen ◽  
Yuichi Mine ◽  
Shan-Hua Wu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Bioactive Glass ◽  
Spray Pyrolysis ◽  
Mesoporous Structure ◽  
High Specific Surface Area ◽  
Process Time ◽  
In Vitro Bioactivity ◽  
One Pot

Mesoporous bioactive glass (MBG) has a high specific surface area, promoting the reaction area, thereby improving the bioactivity; thus, MBG is currently gaining popularity in the biomaterial field. Spray pyrolysis (SP) is a one-pot process that has the advantages of shorter process time and better particle bioactivity, therefore, MBG was prepared by SP process with various polyethylene glycol (PEG, molecular weight ranged from 2000–12,000) and acid (HCl and CH3COOH) additions. In vitro bioactivity and mesoporous properties of the so-obtained MBG were investigated. The experimental results showed that all the MBG exhibited amorphous and mesoporous structure. Increasing the molecular weight of PEG can improve the mesoporous structure and bioactivity of MBG. Whereas optimized MBG was prepared with suitable concentration of PEG and CH3COOH. In the present work, MBG synthesized via spray pyrolysis by adding 5 g of PEG with a molecular weight of 12,000 and 50 mL of CH3COOH exhibited the best in vitro bioactivity and mesoporous structure.

scholarly journals Bioactıve Glass‐Polymer Nanocomposites for Bone Tıssue Regeneration Applicatıons: A Revıew

2019 ◽  
Vol 21 (8) ◽  
pp. 1900287 ◽  
Author(s):  
Melek Erol‐Taygun ◽  
Irem Unalan ◽  
Maizlinda Izwana Binti Idris ◽  
João F. Mano ◽  
Aldo R. Boccaccini
Keyword(s):  
Bioactive Glass ◽  
Bone Tissue ◽  
Polymer Nanocomposites ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration

scholarly journals Silicon Nitride, a Bioceramic for Bone Tissue Engineering: A Reinforced Cryogel System With Antibiofilm and Osteogenic Effects

2021 ◽  
Vol 9 ◽  
Author(s):  
Seunghun S. Lee ◽  
Leanid Laganenka ◽  
Xiaoyu Du ◽  
Wolf-Dietrich Hardt ◽  
Stephen J. Ferguson
Keyword(s):  
Tissue Engineering ◽  
Silicon Nitride ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Space Shuttle ◽  
Biological Effects ◽  
Antibacterial Properties ◽  
Industrial Applications ◽  
System Component ◽  
Mechanical Stiffness

Silicon nitride (SiN [Si3N4]) is a promising bioceramic for use in a wide variety of orthopedic applications. Over the past decades, it has been mainly used in industrial applications, such as space shuttle engines, but not in the medical field due to scarce data on the biological effects of SiN. More recently, it has been increasingly identified as an emerging material for dental and orthopedic implant applications. Although a few reports about the antibacterial properties and osteoconductivity of SiN have been published to date, there have been limited studies of SiN-based scaffolds for bone tissue engineering. Here, we developed a silicon nitride reinforced gelatin/chitosan cryogel system (SiN-GC) by loading silicon nitride microparticles into a gelatin/chitosan cryogel (GC), with the aim of producing a biomimetic scaffold with antibiofilm and osteogenic properties. In this scaffold system, the GC component provides a hydrophilic and macroporous environment for cells, while the SiN component not only provides antibacterial properties and osteoconductivity but also increases the mechanical stiffness of the scaffold. This provides enhanced mechanical support for the defect area and a better osteogenic environment. First, we analyzed the scaffold characteristics of SiN-GC with different SiN concentrations, followed by evaluation of its apatite-forming capacity in simulated body fluid and protein adsorption capacity. We further confirmed an antibiofilm effect of SiN-GC against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as well as enhanced cell proliferation, mineralization, and osteogenic gene upregulation for MC3T3-E1 pre-osteoblast cells. Finally, we developed a bioreactor to culture cell-laden scaffolds under cyclic compressive loading to mimic physiological conditions and were able to demonstrate improved mineralization and osteogenesis from SiN-GC. Overall, we confirmed the antibiofilm and osteogenic effect of a silicon nitride reinforced cryogel system, and the results indicate that silicon nitride as a biomaterial system component has a promising potential to be developed further for bone tissue engineering applications.

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