In vivo skin treatment using two portable plasma devices: Comparison of a direct and an indirect cold atmospheric plasma treatment

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

Download Full-text

The Application of the Cold Atmospheric Plasma-Activated Solutions in Cancer Treatment

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170731115233 ◽

2018 ◽

Vol 18 (6) ◽

pp. 769-775 ◽

Cited By ~ 13

Author(s):

Dayun Yan ◽

Jonathan H. Sherman ◽

Michael Keidar

Keyword(s):

Cancer Treatment ◽

Atmospheric Plasma ◽

Current Status ◽

Cold Atmospheric Plasma ◽

Anti Cancer ◽

Long Time ◽

Key Topics ◽

The Common

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

Download Full-text

Cold atmospheric plasma treatment for diaper dermatitis: A case report

Dermatologic Therapy ◽

10.1111/dth.14739 ◽

2021 ◽

Author(s):

Chenchen Zhang ◽

Jun Zhao ◽

Yamei Gao ◽

Jing Gao ◽

Yongmei Lv

Keyword(s):

Case Report ◽

Plasma Treatment ◽

Atmospheric Plasma ◽

Diaper Dermatitis ◽

Cold Atmospheric Plasma

Download Full-text

Cold atmospheric plasma treatment selectively targets head and neck squamous cell carcinoma cells

International Journal of Molecular Medicine ◽

10.3892/ijmm.2014.1849 ◽

2014 ◽

Vol 34 (4) ◽

pp. 941-946 ◽

Cited By ~ 86

Author(s):

RAFAEL GUERRERO-PRESTON ◽

TAKENORI OGAWA ◽

MAMORU UEMURA ◽

GARY SHUMULINSKY ◽

BLANCA L. VALLE ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Plasma Treatment ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Carcinoma Cells ◽

Neck Squamous Cell Carcinoma ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

Download Full-text

Wettability of SiOx Nanofibrous Mats and Their Modification Using Cold Atmospheric Plasma Treatment

Acta Physica Polonica A ◽

10.12693/aphyspola.140.181 ◽

2021 ◽

Vol 140 (2) ◽

pp. 181-185

Author(s):

M. Domonkos ◽

P. Tichá

Keyword(s):

Plasma Treatment ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

Download Full-text

Microbial Decontamination of Mango and Melon Surface Using a Cold Atmospheric Plasma Treatment

2007 IEEE 34th International Conference on Plasma Science (ICOPS) ◽

10.1109/ppps.2007.4345641 ◽

2007 ◽

Author(s):

Stefano Perni ◽

Gilbert Shama ◽

M. G. Kong

Keyword(s):

Plasma Treatment ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

Download Full-text

Cold atmospheric plasma treatment inhibits growth in colorectal cancer cells

Biological Chemistry ◽

10.1515/hsz-2018-0193 ◽

2018 ◽

Vol 400 (1) ◽

pp. 111-122 ◽

Cited By ~ 10

Author(s):

Christin Schneider ◽

Stephanie Arndt ◽

Julia L. Zimmermann ◽

Yangfang Li ◽

Sigrid Karrer ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Plasma Treatment ◽

Cancer Cells ◽

Ex Vivo ◽

Atmospheric Plasma ◽

Normal Colon ◽

Colon Tissue ◽

Cold Atmospheric Plasma ◽

Colorectal Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

Download Full-text

INNV-13. SYNERGISTIC EFFECT OF COLD ATMOSPHERIC PLASMA IN COMBINATION WITH TEMOZOLOMIDE TO TREAT GLIOBLASTOMA IN A NON-INVASIVE MOUSE XENOGRAFT MODEL

Neuro-Oncology ◽

10.1093/neuonc/noaa215.496 ◽

2020 ◽

Vol 22 (Supplement_2) ◽

pp. ii119-ii119

Author(s):

Manish Adhikari ◽

Vikas Soni ◽

Simonyan Hayk ◽

Colin Young ◽

Jonathan Sherman ◽

...

Keyword(s):

Combination Therapy ◽

Tumor Volume ◽

Bioluminescence Imaging ◽

Xenograft Model ◽

Atmospheric Plasma ◽

Minimal Effect ◽

Cold Atmospheric Plasma ◽

Mouse Xenograft Model ◽

In Vivo Bioluminescence Imaging

Abstract INTRODUCTION A primary limitation in anti-cancer therapy is the resistance of cancer cells to chemotherapeutic drugs. However, combination therapy may be an effective approach for reducing drug derived toxicity and evading drug resistance, resulting in improved clinical treatment of cancer. Our prior work demonstrated effective treatment of glioblastoma (GBM) with cold atmospheric plasma (CAP) technology with minimal effect to normal cells. Consequently, CAP may serve as a strong candidate for combination therapy with the classical antineoplastic alkylating agent Temozolomide (TMZ) to treat GBM. OBJECTIVES To determine the in vivo co-efficacy of CAP and TMZ to “sensitize” GBM. METHODS An in vivo study was performed using the CAP jet device (He-gas) to determine the effect of combined CAP–TMZ treatment. U87MG-luc glioblastoma cells were implanted intracranially in athymic nude NU(NCr)-Foxn1nu/immunodeficient mice. He-CAP (or control He alone) was non-invasively applied over the skin for 60sec to developed tumors on the first day of the treatment followed with 6.5 mg/kg TMZ or vehicle control treatment for 5 days for two weeks (n=5/group). In vivo bioluminescence imaging was used to monitor tumor volume on the 6th, 9th and 13th treatment day. RESULTS In vivo bioluminescence imaging revealed a marked 8.0±3.2 fold increase in tumor volume in control animals (He-vehicle). Treatment with He-TMZ (6.7±2.5 fold) or CAP-vehicle (4.8±1.7 fold) in isolation had minimal effect in preventing tumor growth. However, combined CAP-TMZ co-treatment virtually prevented increases in tumor volume over 2 weeks (1.8±0.2 fold). CONCLUSIONS Collectively, these findings indicate an effective synergistic treatment method for GBM combining CAP with TMZ. Future investigations look to incorporate radiation into the treatment regimen as well as primary GBM cell models.

Download Full-text