Ratiometric probe with optimized permeability for visualizing lysosomal acidification during autophagy

The use of carbon dots (CDs) with dual emission based on ratiometric fluorescence has been attracting attention in recent times for more accurate ion detection since they help avoid interference from background noise, probe concentration, and complexity. Herein, novel dual-emission nitrogen-doped CDs (NCDs) were prepared by a simple method for Cu2+ and ClO- detection. The NCDs showed excellent anti-interference ability and selectivity for different emissions. In addition, a good linear relationship was observed between the fluorescence intensity (FI) of the NCD solutions in different emissions with Cu2+ (0–90 μM) and ClO- (0–75 μM). The limits of both Cu2+ detection and ClO- were very low, at 17.7 and 11.6 nM, respectively. The NCDs developed herein also showed a good recovery rate in water for Cu2+ and ClO− detection. Hence, they are expected to have a more extensive application prospect in real samples.

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A mitochondrial-targeted ratiometric probe for detecting intracellular H2S with high photostability

Chinese Chemical Letters ◽

10.1016/j.cclet.2020.12.044 ◽

2020 ◽

Author(s):

Dandan Bu ◽

Yuying Wang ◽

Na Wu ◽

Wei Feng ◽

Donghui Wei ◽

...

Keyword(s):

Ratiometric Probe

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Presenilins: A novel link between intracellular calcium signaling and lysosomal function?

The Journal of Cell Biology ◽

10.1083/jcb.201206003 ◽

2012 ◽

Vol 198 (1) ◽

pp. 7-10 ◽

Cited By ~ 14

Author(s):

Ilya Bezprozvanny

Keyword(s):

Calcium Signaling ◽

Intracellular Calcium ◽

Transmembrane Proteins ◽

Catalytic Subunit ◽

Familial Alzheimer’S Disease ◽

Lysosomal Function ◽

Cell Biol ◽

Intracellular Calcium Signaling ◽

Lysosomal Acidification ◽

In Cells

Mutations in presenilins (PS), transmembrane proteins encoding the catalytic subunit of γ-secretase, result in familial Alzheimer’s disease (FAD). Several studies have identified lysosomal defects in cells lacking PS or expressing FAD-associated PS mutations, which have been previously attributed to a function for PS in lysosomal acidification. Now, in this issue, Coen et al. (2012. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201201076) provide a series of results that challenge this idea and propose instead that presenilins play a role in calcium-mediated lysosomal fusion.

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Inhibition of Glycogen Synthase Kinase-3 Ameliorates β-Amyloid Pathology and Restores Lysosomal Acidification and Mammalian Target of Rapamycin Activity in the Alzheimer Disease Mouse Model

Journal of Biological Chemistry ◽

10.1074/jbc.m112.409250 ◽

2012 ◽

Vol 288 (2) ◽

pp. 1295-1306 ◽

Cited By ~ 122

Author(s):

Limor Avrahami ◽

Dorit Farfara ◽

Maya Shaham-Kol ◽

Robert Vassar ◽

Dan Frenkel ◽

...

Keyword(s):

Alzheimer Disease ◽

Mouse Model ◽

Glycogen Synthase ◽

Mammalian Target Of Rapamycin ◽

Glycogen Synthase Kinase ◽

Target Of Rapamycin ◽

Glycogen Synthase Kinase 3 ◽

Amyloid Pathology ◽

Β Amyloid ◽

Lysosomal Acidification

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Host–guest-recognition-based polymer brush-functionalized mesoporous silica nanoparticles loaded with conjugated polymers: A facile FRET-based ratiometric probe for Hg 2+

Microporous and Mesoporous Materials ◽

10.1016/j.micromeso.2015.07.014 ◽

2015 ◽

Vol 218 ◽

pp. 137-143 ◽

Cited By ~ 14

Author(s):

Jie Sha ◽

Yajiao Song ◽

Bingxin Liu ◽

Changli Lü

Keyword(s):

Mesoporous Silica ◽

Conjugated Polymers ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Polymer Brush ◽

Functionalized Mesoporous Silica ◽

Ratiometric Probe

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Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

Dalton Transactions ◽

10.1039/c7dt02418e ◽

2017 ◽

Vol 46 (40) ◽

pp. 13957-13965 ◽

Cited By ~ 9

Author(s):

Shuoren Du ◽

Javier Hernández-Gil ◽

Hao Dong ◽

Xiaoyu Zheng ◽

Guangming Lyu ◽

...

Keyword(s):

Intracellular Ph ◽

Quantitative Imaging ◽

Living Cells ◽

Ph Sensitive ◽

Upconversion Nanoparticles ◽

Imaging Probe ◽

Ph Imaging ◽

Ratiometric Probe ◽

Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

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