Synthesis of (3 S ,4 S ,5 S )-trihydroxylpiperidine derivatives as enzyme stabilizers to improve therapeutic enzyme activity in Fabry patient cell lines

2018 ◽  
Vol 144 ◽  
pp. 626-634 ◽  
Author(s):  
Huang-Yi Li ◽  
Jay-Der Lee ◽  
Chiao-Wen Chen ◽  
Ying-Chieh Sun ◽  
Wei-Chieh Cheng
Keyword(s):  
Enzyme Activity ◽  
Cell Lines ◽  
Fabry Patient ◽  
Patient Cell

The pharmacological chaperone 1-deoxygalactonojirimycin increases α-galactosidase A levels in Fabry patient cell lines

2009 ◽  
Vol 32 (3) ◽  
pp. 424-440 ◽  
Author(s):  
E. R. Benjamin ◽  
J. J. Flanagan ◽  
A. Schilling ◽  
H. H. Chang ◽  
L. Agarwal ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fabry Patient ◽  
Pharmacological Chaperone ◽  
Patient Cell

Synthesis, cytotoxicity, antioxidant and antimicrobial activity of indole based novel small molecule

2020 ◽  
Vol 17 ◽  
Author(s):  
Aslıhan Kurt-Kızıldoğan ◽  
Çiğdem Otur ◽  
Can Yılmaz ◽  
Sevki Arslan ◽  
Dogukan Mutlu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Antimicrobial Properties ◽  
Cancer Cell Lines ◽  
Coupling Reactions ◽  
Human Liver Cancer ◽  
Glutathione S Transferases ◽  
Antioxidant And Antimicrobial Activity

Background:: Indoles probably represent one of the most important heterocyclic structures that have been attracting the interest of many scientists in drug discovery. Methods:: Pd-catalyst Sonogashira coupling reactions, MTT Assay, Antioxidant capacity test, Antimicrobial test, GST enzyme activity test. Results and Discussion:: 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties. It displayed significant induction in glutathione S-transferases (GST) enzyme activity in human liver cancer cell lines (HepG2), but cytotoxic effect on all tested cancer cell lines could not be observed. Conclusion:: All of these results showed that 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties without cytotoxic effect, which could make it a promising active component.

scholarly journals Glutathione and related enzyme activity in human lung cancer cell lines

1988 ◽  
Vol 58 (4) ◽  
pp. 437-440 ◽  
Author(s):  
J Carmichael ◽  
JB Mitchell ◽  
N Friedman ◽  
AF Gazdar ◽  
A Russo
Keyword(s):  
Lung Cancer ◽  
Enzyme Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Lung ◽  
Cancer Cell Lines ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Human Lung Cancer Cell ◽  
Related Enzyme

scholarly journals Two distinct genetic loci regulating class II gene expression are defective in human mutant and patient cell lines.

1988 ◽  
Vol 7 (7) ◽  
pp. 1965-1972 ◽  
Author(s):  
Z. Yang ◽  
R. S. Accolla ◽  
D. Pious ◽  
B. J. Zegers ◽  
J. L. Strominger
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Class Ii ◽  
Genetic Loci ◽  
Patient Cell

scholarly journals Role of Resveratrol in Transmitochondrial AMD RPE Cells

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 159
Author(s):  
Sonali Nashine ◽  
Anthony B. Nesburn ◽  
Baruch D. Kuppermann ◽  
Maria Cristina Kenney
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Viability ◽  
Macular Degeneration ◽  
Cell Lines ◽  
Blood Platelets ◽  
Reactive Oxygen ◽  
Age Related Macular Degeneration ◽  
Oxygen Species ◽  
Positive Effects ◽  
Patient Cell

Resveratrol is a phytoalexin, stilbenoid compound with antioxidant properties attributable to its bioactive trans-resveratrol content. This study characterized the effects of over-the-counter (OTC) resveratrol nutritional supplements and a HPLC-purified resveratrol formulation, in human transmitochondrial age-related macular degeneration (AMD) retinal pigment epithelial (RPE) patient cell lines. These cell lines, which were created by fusing blood platelets obtained from dry and wet AMD patients with mitochondria-deficient (Rho0) ARPE-19 cells, had identical nuclei (derived from ARPE-19 cells) but different mitochondria that were derived from AMD patients. After resveratrol treatment, the levels of cell viability and reactive oxygen species production were measured. Results demonstrated that treatment with different resveratrol formulations improved cell viability and decreased reactive oxygen species generation in each AMD patient cell line. Although further studies are required to establish the cytoprotective potential of resveratrol under different physiological conditions, this novel study established the positive effects of OTC resveratrol supplements in macular degeneration patient cybrid cell lines in vitro.

Regulation of PG Synthase by EGF and PDGF in Human Oral, Breast, Stomach, and Fibrosarcoma Cancer Cell Lines

1994 ◽  
Vol 73 (8) ◽  
pp. 1407-1415 ◽  
Author(s):  
C.Y. Yang ◽  
C.L. Meng
Keyword(s):  
Enzyme Activity ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Platelet Derived Growth Factor ◽  
Moderate Degree ◽  
Epidermal Growth

Prostaglandins may inhibit or promote tumor cell replication, depending on the cell system that is investigated. In our laboratory, we have established and characterized four different specific human cancer cell lines. The objectives of this study were to examine and compare the prostaglandin endoperoxide synthase (PG synthase, EC 1.14.99.1) activity of these cell lines by measuring the conversion of arachidonate to 3H-PGE2 and 3H-PGF2α. We found that the oral epidermal carcinoma cell line (OEC-M1) had a moderate degree of PG synthase activity. Enzyme activity could be partially blocked (statistically significant) by the addition of epidermal growth factor (EGF) at 20 ng/mL and almost completely inhibited by platelet-derived growth factor at (PDGF) 20 mU/mL. By contrast, we discovered that the human breast adenocarcinama cell line (BC-M1) did not contain significant PG synthase, and enzyme activity could be significantly activated by the addition of epidermal growth factor at 20 ng/mL and platelet-derived growth factor at 20 mU/mL. We also found that the human stomach adenocarcinoma cell line (SCM-1) had a significant amount of PG synthase activity, and these PG synthase activities were not activated or inhibited by EGF at 20 ng/mL or PDGF at 20 mU/mL. Furthermore, the human fibrosarcoma (FS-Ml) cell line also contained a moderate degree of PG synthase activity, which could be significantly inhibited by PDGF at 20 mU/mL but was not inhibited by EGF at 20 ng/mL. The results suggest that EGF and PDGF may be involved in the regulation of the PG synthase activities of human oral, breast, stomach, and fibrosarcoma cancer cells.

scholarly journals AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

2012 ◽  
Vol 27 (12) ◽  
pp. 855-860 ◽  
Author(s):  
Celestino Prospero de Souza Sobrinho ◽  
Alfredo Gragnani ◽  
Ivan Dunshee Abranches Oliveira Santos ◽  
Andrea Fernandes Oliveira ◽  
Monica Vanucci Nunes Lipay ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Enzyme Activity ◽  
Cell Lines ◽  
Telomerase Activity ◽  
Melanoma Cells ◽  
Control Group ◽  
No Inhibition

PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.

Sign in / Sign up

Export Citation Format

Share Document