Conjugated polyamines are involved in conformation stability of plasma membrane from maturing maize grain embryos under drought stress

2021 ◽  
pp. 104726
Author(s):  
Hongyang Du ◽  
Dongxiao Liu ◽  
Guting Liu ◽  
Huaipan Liu ◽  
Haili Sun ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Conjugated Polyamines ◽  
Maize Grain ◽  
Conformation Stability

scholarly journals Conjugated Polyamines in Root Plasma Membrane Enhanced the Tolerance of Plum Seedling to Osmotic Stress by Stabilizing Membrane Structure and Therefore Elevating H+-ATPase Activity

2022 ◽  
Vol 12 ◽  
Author(s):  
Hongyang Du ◽  
Benxue Chen ◽  
Qiang Li ◽  
Huaipan Liu ◽  
Ronald Kurtenbach
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Osmotic Stress ◽  
Atpase Activity ◽  
Membrane Structure ◽  
Membrane Damage ◽  
Cellular Level ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
Conjugated Polyamines

Polyamines are small positively charged molecules in plants and play important functions in many biological processes under various environmental stresses. One of the most confounding problems relating to polyamines (PAs) in stresses is the lack of understanding of the mechanisms underlying their function(s). Furthermore, a limited number of studies have addressed this issue at the sub-cellular level, especially in tree plants under drought stress. Therefore, in this research, by simulating natural drought stress with polyethylene glycol (PEG) osmotic stress, the relationship between the levels of conjugated polyamines and the activity of H+-ATPase in the plasma membrane was elucidated with the roots of two plum (Prunus salicina L.) cultivars, which were different in drought tolerance, as experimental materials. Furthermore, free PA levels and the activities of S-adenosylmethionine decarboxylase (SAMDC) and transglutaminase (TGase), which were closely associated with the levels of free and conjugated PAs, were also detected. Results showed that under osmotic stress, the increases of the levels of non-covalently conjugated (non-CC) spermidine (Spd) and spermine (Spm), covalently conjugated (CC) putrescine (Put) and Spd in the plasma membrane of drought-tolerant Ganli No. 5 were more significant than those of drought-sensitive Suli No. 3, indicating that these conjugated PAs might be involved in the tolerance of plum seedlings to stress. Furthermore, the conjugated PAs were closely correlated with plum seedling growth, water retention capacity, plasma membrane damage degree, and hydrogen (H+)-ATPase activity in the plasma membrane. To get more complementary pieces of evidence, we subjected plum seedlings to combined treatments of PEG and exogenous PA (Spd and Spm), and an inhibitor of SAMDC [methylglyoxal-bis (guanylhydrazone), (MGBG)] or TGase (o-phenanthroline). These results collectively suggested that non-CC Spd and Spm, CC Put and Spd in plasma membrane might function in enhancing the tolerance of plum seedlings to osmotic stress by stabilizing membrane structure and therefore elevating H+-ATPase activity.

scholarly journals Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ren Li ◽  
Jinfang Wang ◽  
Shuangtao Li ◽  
Lei Zhang ◽  
Chuandong Qi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Drought Stress Tolerance ◽  
Plasma Membrane Intrinsic Proteins

Molecular cloning of a plasma membrane aquaporin in Stipa purpurea , and exploration of its role in drought stress tolerance

Gene ◽  
2018 ◽  
Vol 665 ◽  
pp. 41-48 ◽  
Author(s):  
Qian Chen ◽  
Shihai Yang ◽  
Xiangxiang Kong ◽  
Chuntao Wang ◽  
Nan Xiang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Molecular Cloning ◽  
Drought Stress Tolerance ◽  
Stipa Purpurea

The plasma membrane NADPH oxidase OsRbohA plays a crucial role in developmental regulation and drought-stress response in rice

2015 ◽  
Vol 156 (4) ◽  
pp. 421-443 ◽  
Author(s):  
Xiang Wang ◽  
Mao-Mao Zhang ◽  
Ya-Jing Wang ◽  
Yin-Tao Gao ◽  
Ri Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Stress Response ◽  
Nadph Oxidase ◽  
Crucial Role ◽  
Developmental Regulation

Changes in H+-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress

2014 ◽  
Vol 75 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Hongyang Du ◽  
Xinguo Zhou ◽  
Qinghua Yang ◽  
Huaipan Liu ◽  
Ronald Kurtenbach
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Atpase Activity ◽  
Wheat Embryos ◽  
Short Time

scholarly journals Improving Maize Grain Yield under Drought Stress and Non-stress Environments in Sub-Saharan Africa using Marker-Assisted Recurrent Selection

Crop Science ◽  
2016 ◽  
Vol 56 (1) ◽  
pp. 344-353 ◽  
Author(s):  
Yoseph Beyene ◽  
Kassa Semagn ◽  
Jose Crossa ◽  
Stephen Mugo ◽  
Gary N. Atlin ◽  
...  
Keyword(s):  
Drought Stress ◽  
Grain Yield ◽  
Recurrent Selection ◽  
Sub Saharan Africa ◽  
Maize Grain Yield ◽  
Stress Environments ◽  
Maize Grain ◽  
Sub Saharan

scholarly journals A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress

2012 ◽  
Vol 45 (2) ◽  
pp. 104-114 ◽  
Author(s):  
J. Zhang ◽  
D. Li ◽  
D. Zou ◽  
F. Luo ◽  
X. Wang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Seedling Development ◽  
Gene Encoding

scholarly journals The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley

2019 ◽  
Vol 20 (17) ◽  
pp. 4111 ◽  
Author(s):  
Kangfeng Cai ◽  
Huaizhou Gao ◽  
Xiaojian Wu ◽  
Shuo Zhang ◽  
Zhigang Han ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transcript Level ◽  
Genotypic Difference ◽  
K Uptake ◽  
Significant Difference ◽  
Specific Manner ◽  
Barley Genotypes

In this work, the effect of drought on K+ uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+ uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+ contents and drought tolerance levels between the contrasting barley genotypes. The role of K+ transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+ uptake (HvHAK1, HvHAK5, HvKUP1, HvKUP2 and HvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+ transporters and channels is critical for root K+ uptake and root to shoot K+ translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+ efflux and K+ influx under drought stress, which was further confirmed by the significant up-regulation of HvHA1 and HvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+ transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+ transporters and channels in root epidermis and stele.

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