Lactobacillus plantarum culture supernatants improve intestinal tissue exposed to deoxynivalenol

2017 ◽  
Vol 69 (8) ◽  
pp. 666-671 ◽  
Author(s):  
L.G. Maidana ◽  
J. Gerez ◽  
F. Pinho ◽  
S. Garcia ◽  
A.P.F.L. Bracarense
Keyword(s):  
Lactobacillus Plantarum ◽  
Intestinal Tissue ◽  
Culture Supernatants

Genomic diversity and immunomodulatory activity of Lactobacillus plantarum isolated from dairy products

2017 ◽  
Vol 8 (4) ◽  
pp. 597-604 ◽  
Author(s):  
M. Zago ◽  
E. Scaltriti ◽  
B. Bonvini ◽  
M.E. Fornasari ◽  
G. Penna ◽  
...  
Keyword(s):  
Immune Response ◽  
Lactobacillus Plantarum ◽  
Dairy Products ◽  
Genomic Diversity ◽  
Immunomodulatory Activity ◽  
Preclinical Models ◽  
Riboflavin Production ◽  
Commercial Use ◽  
Different Strains ◽  
Culture Supernatants

In this study, we aimed to investigate some functional characteristics and the immunomodulatory properties of three strains of Lactobacillus plantarum of dairy origin which, in a previous screening, showed to be candidate probiotics. Genome sequencing and comparative genomics, which confirmed the presence of genes involved in folate and riboflavin production and in the immune response of dendritic cells (DCs), prompted us to investigate the ability of the three strains to accumulate the two vitamins and their immunomodulation properties. The ability of the three strains to release antioxidant components in milk was also investigated. Small amounts of folate and riboflavin were produced by the three strains, while they showed a good antioxidant capacity in milk with FRAP method. The immune response experiments well correlated with the presence of candidate genes influencing in DCs cytokine response to L. plantarum. Specifically, the amounts of secreted cytokins by DCs after stimulation with cells of Lp790, Lp813 and Lp998 resulted pro-inflammatory whereas stimulation with culture supernatants (postbiotics) inhibited the release of interleukin (IL)-12p70 and increased the release of the anti-inflammatory IL-10 cytokine. This study adds further evidence on the importance of L. plantarum in human health. Understanding how probiotics (or postbiotics) work in preclinical models can allow a rational choice of the different strains for clinical and/or commercial use.

scholarly journals An agr-Like Two-Component Regulatory System in Lactobacillus plantarum Is Involved in Production of a Novel Cyclic Peptide and Regulation of Adherence

2005 ◽  
Vol 187 (15) ◽  
pp. 5224-5235 ◽  
Author(s):  
Mark H. J. Sturme ◽  
Jiro Nakayama ◽  
Douwe Molenaar ◽  
Yoshiko Murakami ◽  
Ryoko Kunugi ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Growth Phase ◽  
Time Course ◽  
Cyclic Peptide ◽  
Expression System ◽  
Peptide Sequence ◽  
Wild Type ◽  
Transcription Analysis ◽  
Genes Encoding ◽  
Culture Supernatants

ABSTRACT We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a response regulator-defective mutant (ΔlamA) in an adherence assay showed that lam regulates adherence of L. plantarum to a glass surface. Global transcription analysis of the wild-type and ΔlamA strains in early, mid-, and late log phase of growth was performed using a clone-based microarray. Remarkably, only a small set of genes showed significant differences in transcription profiles between the wild-type and lamA mutant strains. The microarray analysis confirmed that lamBDCA is autoregulatory and showed that lamA is involved in regulation of expression of genes encoding surface polysaccharides, cell membrane proteins, and sugar utilization proteins. The lamBD genes encoding the putative autoinducing peptide precursor (LamD) and its processing protein (LamB) were overexpressed using the nisin-controlled expression system, and culture supernatants were analyzed by liquid chromatography/mass spectrometry (LC/MS) to identify overproduced LamD-derived peptides. In this way, a cyclic thiolactone pentapeptide that possesses a ring structure similar to those of autoinducing peptides of the staphylococcal agr system was identified. The peptide was designated LamD558, and its sequence (CVGIW) matched the annotated precursor peptide sequence. Time course analysis of wild-type culture supernatants by LC/MS indicated that LamD558 production was increased markedly from mid-log to late log growth phase. This is the first example of an agr-like system in nonpathogenic bacteria that encodes a cyclic thiolactone autoinducing peptide and is involved in regulation of adherence.

scholarly journals Histopathological and ultrastructural findings induced by heat-inactivated Lactobacillus plantarum and the culture supernatant on the intestinal mucosa of piglets: an ex vivo approach

2019 ◽  
Vol 71 (1) ◽  
pp. 11-20
Author(s):  
L.G. Maidana ◽  
J. Gerez ◽  
F. Pinho ◽  
S. Garcia ◽  
A.P. Bracarense
Keyword(s):  
Lactobacillus Plantarum ◽  
Intestinal Mucosa ◽  
Morphometric Analysis ◽  
Culture Medium ◽  
Culture Supernatant ◽  
Ex Vivo ◽  
Intestinal Morphology ◽  
Control Group ◽  
Culture Supernatants ◽  
Apical Necrosis

ABSTRACT In the present study, histological, morphometrical and ultrastructural analysis were performed to investigate intestinal mucosa changes in piglets jejunal explants exposed to two concentration of heat-inactivated Lactobacillus plantarum and their respective culture supernatants. Jejunal explants were incubated for 4 hours in DMEM culture medium with a) only culture medium (control group), b) heat-inactivated Lactobacillus plantarum strain1 - LP1 (1.1 x 108CFU/ml), c) heat-inactivated Lactobacillus plantarum strain2 - LP2 (2.0 x 109CFU/ml), d) heat-inactivated Lactobacillus plantarum strain1 culture supernatant (CS1), and e) heat-inactivated Lactobacillus plantarum strain2 culture supernatant (CS2). Explants exposed to heat-inactivated L. plantarum strain 1 and 2 showed multifocal to difuse villi atrophy, villi apical necrosis and enterocyte flattening. Morphological assessment revealed similar results with bacterial adhesion to mucus and intestinal epithelial cells and, morphometric analysis showed a decreased villi height compared to the control group. Alterations in explants treated with the culture supernatant of both strains include mild villi atrophy and mild enterocyte apical necrosis. Morphological assesment reveled numerous well delineated villi and, morphometric analysis showed a significant increase in villi height compared to the control group. In general, exposure to the culture supernatants improved the intestinal morphology.

The absorption characteristics of bifendate solid dispersion in rat intestinal tissue

2009 ◽  
Vol 00 (00) ◽  
pp. 090810023429058-9
Author(s):  
Juan Cheng ◽  
Zheng Hong Wu ◽  
Qineng Neng Ping ◽  
Bogang Wang ◽  
Junjun Lu
Keyword(s):  
Solid Dispersion ◽  
Intestinal Tissue ◽  
Absorption Characteristics

Differential Detection of Single-Chain and Two-Chain Urokinase-Type Plasminogen Activator by a New Immunoadsorbent-Amidolytic Assay (IAA)

1986 ◽  
Vol 56 (03) ◽  
pp. 407-410 ◽  
Author(s):  
Angelo Corti ◽  
Maria Luisa Nolli ◽  
Giovanni Cassani
Keyword(s):  
Plasminogen Activator ◽  
Carcinoma Cell ◽  
Differential Detection ◽  
Single Chain ◽  
Response Curves ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Before And After ◽  
Culture Supernatants ◽  
Human Epidermoid Carcinoma

SummaryA new immunoadsorbent-amidolytic assay (IAA) for the specific differential detection of two-chain urokinase-type plasminogen activator (tcu-PA) and its single-chain precursor (scu-PA) in cell culture supernatants has been developed. The assay combines the selectivity of immunoassays with the specificity of enzyme activity assays exploiting both the antigenic and enzymatic properties of the two proteins. tcu-PA and scu-PA are selectively immunoadsorbed on the wells of a microtiterplate coated with the monoclonal antibody 5B4 and tested for enzymatic activity before and after activation by plasmin treatment. Both proteins are determined with similar efficiency since overlapping dose-response curves were obtained in the range between 12.5-200 ng/ml. The assay has been used to determine tcu-PA and scu-PA in A431 human epidermoid carcinoma cell supernatants. The analytical recoveries for tcu-PA and scu-PA added to A431 cell supernatants were 95.2% and 96.9% respectively. The intra- and inter-assay variations (CV) were 5.5% and 9.0% for tcu-PA and 9.7% and 9.8% for scu-PA respectively.

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