Vitamin C, Vitamin E and oxidative stress: effect on endometrial cell gene exfpression and proliferation in vitro

CAD is recognized as a multifactorial disease that is influenced by environmental and genetic factors. This study aimed to evaluate the levels of lipid parameters, oxidative stress and antioxidant markers in subjects with CAD compared to their age & sex matched controls and to analyze the relationship between atherogenic Index and oxidative stress among them 62 clinically proved CAD patients and 62 healthy age and sex matched subjects without CAD were selected for this study. 5 ml of fasting venous blood was collected from all the subjects and investigations such as FPG, lipid profile, oxidative markers Malondialdehyde (MDA), F2 isoprostanes (F2iso) and antioxidants glutathione S-transferase (GST), superoxide dismutase (SOD), vitamin-C, vitamin-E were performed. This study showed that levels of lipid parameters total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and AI were significantly higher whereas high density lipoprotein cholesterol (HDL-c) were significantly low in CAD patients compared to normal controls. Oxidative stress markers MDA and F2 Isoprostanes level were significantly high, whereas enzymatic antioxidants GST and SOD and non-enzymatic antioxidants Vitamin-C and Vitamin-E levels were significantly low in CAD patients. Oxidative stress markers were found to significantly influence the AI. Results of this study showed that oxidative stress markers F2iso and MDA and antioxidants GST, VIT-C and VIT-E are found to influence the atherogenic index significantly.

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Vitamins E and C Alleviate the Germ Cell Loss and Oxidative Stress in Cryptorchidism When Administered Separately but Not When Combined in Rats

ISRN Pharmacology ◽

10.5402/2012/843569 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Ayobami Oladele Afolabi ◽

Olaolu Opeyemi Olotu ◽

Isiaka Abdullateef Alagbonsi

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Germ Cell ◽

Cell Count ◽

Cell Loss ◽

Vitamins E And C ◽

Testicular Weight ◽

Significant Difference ◽

And Oxidative Stress

The antioxidant effects of vitamins C and E on cryptorchidism-induced oxidative stress were investigated in male Sprague-Dawley rats. Forty rats (200–250 g) were randomly divided in a blinded fashion into five groups (). Group 1 was sham operated and treated with vehicle (corn-oil, 10 mL/kg). Groups 2, 3, 4, and 5 were rendered unilaterally cryptorchid and treated with vehicle (10 mL/kg), vitamin E solution (75 mg/kg), vitamin C solution (1.25 g/kg), and combination of vitamin E (75 mg/kg) and vitamin C (1.25 g/kg) solutions, respectively. Germ cell count, superoxide dismutase (SOD), total protein (TP), and testicular weight (TW) were lower, but malondialdhyde (MDA) was higher in the cryptorchid rats than the sham-operated rats. When administered separately, vitamins C and E increased germ cell count, SOD, TP, and TW but did not reduce MDA in the cryptorchid rats when compared to the vehicle-treated cryptorchid rats. However, there was no significant difference in these parameters between vehicle-treated and combined vitamins C- and E-treated rats. This suggests that vitamins E and C alleviate the germ cell loss and oxidative stress in cryptorchidism when administered separately but not when combined in rats.

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Vitamin E Supplementation and Markers of Oxidative Stress in Indian Acute Myocardial Infarction Patients

Asian Journal of Medical Sciences ◽

10.3126/ajms.v5i2.8430 ◽

2013 ◽

Vol 5 (2) ◽

pp. 46-53 ◽

Cited By ~ 1

Author(s):

Ijen Bhattacharya ◽

Rahul Saxena ◽

Raj Saxena ◽

Alok Milton Lal

Keyword(s):

Oxidative Stress ◽

Myocardial Infarction ◽

Uric Acid ◽

Vitamin E ◽

Vitamin C ◽

Plasma Vitamin ◽

Blood Samples ◽

Markers Of Oxidative Stress ◽

Vitamin E Supplementation

Background: Reactive oxygen species have been identified as mediators of cell injury in a variety of cardiovascular complications including Myocardial Infarction (MI). It is conceivable that vitamin E supplementation can be used therapeutically due to its role in ameliorating antioxidant status and free radicals scavenging activity. Aim: Therefore, the present study was undertaken to assess the markers of oxidative stress i.e. erythrocyte glutathione peroxidase (GSHPx) & malondialdehyde (MDA); plasma vitamin C, E, A and uric acid level in the blood samples of MI patients and to investigate the effect of in-vitro vitamin E supplementation in ameliorating the levels of these antioxidants in the blood sample of MI patients. Material & Method: 60 MI subjects (age group 30-60 years) were taken for the study and 60 healthy individuals served as controls. In-vitro vitamin E supplementation in the blood samples of MI subjects were performed and above mentioned parameters were estimated by using standard methods. Data was compared statistically by using student t-test. Result: Vitamin E supplementation brought about an improved antioxidants status with significantly raised vitamin C, E, A and GSHPx levels (p<0.05, p<0.001), and simultaneously depleted level of erythrocyte MDA (p<0.001) in blood samples of MI subjects. However, plasma uric acid levels remain unaltered (p<0.1). Conclusion: These findings further support the preventive and cardio protective role of vitamin E supplementation in reducing oxidative stress levels in the blood samples of MI patients. DOI: http://dx.doi.org/10.3126/ajms.v5i2.8430 Asian Journal of Medical Science, Volume-5(2) 2014: 46-53

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The effects of large doses of vitamin C and vitamin E on nerve injury, neurotrophic and oxidative stress in patients with acute craniocerebral injury

Journal of Acute Disease ◽

10.4103/2221-6189.233014 ◽

2018 ◽

Vol 7 (2) ◽

pp. 69

Author(s):

Cheng Zhang ◽

Jian-Ming Li ◽

Jun-Lin Hu ◽

Xia Zhou

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Nerve Injury ◽

Craniocerebral Injury ◽

And Oxidative Stress

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Effects of Dietary Supplementation with Vitamin C and Vitamin E and Their Combination on Growth Performance, Some Biochemical Parameters, and Oxidative Stress Induced by Copper Toxicity in Broilers

Biological Trace Element Research ◽

10.1007/s12011-014-9926-6 ◽

2014 ◽

Vol 158 (2) ◽

pp. 186-196 ◽

Cited By ~ 20

Author(s):

Miyase Cinar ◽

Ebru Yildirim ◽

A.Arzu Yigit ◽

Ilkay Yalcinkaya ◽

Ozkan Duru ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Growth Performance ◽

Biochemical Parameters ◽

Copper Toxicity ◽

Dietary Supplementation ◽

And Oxidative Stress

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Effects of exogenous vitamins A, C, and E and NADH supplementation on proliferation, cytokines release, and cell redox status of lymphocytes from healthy aged subjects

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2016-0201 ◽

2017 ◽

Vol 42 (6) ◽

pp. 579-587 ◽

Cited By ~ 13

Author(s):

Samia Bouamama ◽

Hafida Merzouk ◽

Amel Medjdoub ◽

Amel Merzouk-Saidi ◽

Sid Ahmed Merzouk

Keyword(s):

Oxidative Stress ◽

Cell Proliferation ◽

Vitamin E ◽

Vitamin C ◽

The Elderly ◽

Redox Status ◽

Age Related ◽

Immune Alterations ◽

Cell Redox Status

Aging is an inevitable biological event that is associated with immune alterations. These alterations are related to increased cellular oxidative stress and micronutrient deficiency. Antioxidant supplementation could improve these age-related abnormalities. The aim of this study was to determine in vitro effects of vitamin A, vitamin C, vitamin E, and nicotinamide adenine dinucleotide (NADH) on T cell proliferation, cytokine release, and cell redox status in the elderly compared with young adults. Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were cultured in vitro and stimulated with concanavalin A in the presence or absence of vitamins. Cell proliferation was determined by conducting MTT assays, and based on interleukin-2 and interleukin-4 secretions. Cell oxidant/antioxidant balance was assessed by assaying reduced glutathione (GSH), malondialdehyde, carbonyl protein levels, and catalase activity. The present study demonstrated that T-lymphocyte proliferation was decreased with aging and was associated with cytokine secretion alterations, GSH depletion, and intracellular oxidative stress. In the elderly, vitamin C, vitamin E, and NADH significantly improved lymphocyte proliferation and mitigated cellular oxidative stress, whereas vitamin A did not affect cell proliferation or cell redox status. In conclusion, vitamin C, vitamin E, and NADH supplementation improved T-lymphocytes response in the elderly, and could contribute to the prevention of age-related immune alterations. Consumption of food items containing these vitamins is recommended, and further investigation is necessary to evaluate the effect of vitamin supplementation in vivo.

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Antioxidant pretreatment enhances umbilical cord derived stem cells survival in response to thermal stress in vitro

Regenerative Medicine ◽

10.2217/rme-2019-0090 ◽

2020 ◽

Vol 15 (3) ◽

pp. 1441-1453

Author(s):

Ramla Ashfaq ◽

Azra Mehmood ◽

Amna Ramzan ◽

Intzar Hussain ◽

Moazzam Nazeer Tarar ◽

...

Keyword(s):

Oxidative Stress ◽

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Thermal Stress ◽

Vitamin E ◽

Vitamin C ◽

Heat Injury ◽

Injury Assessment

Aim: Pretreatment of stem cells with antioxidants accelerates their ability to counter oxidative stress and is associated with the overall therapeutic outcome of their transplantation. Material & methods: Wharton Jelly derived mesenchymal stem cells (WJMSCs) were cultured and pretreated with various doses of antioxidants; Vitamin C (Vit C), Vitamin E (Vit E), Vitamin D3 (Vit D3) and their Cocktail, followed by exposure to in vitro heat injury. Assessment of WJMSCs survival, paracrine release, in vitro wound healing and expression of angiogenic and survival markers was conducted. Results: The results displayed an enhanced survival of WJMSCs especially in the case of Cocktail priming. Conclusion: Our data suggest that antioxidant pretreatment of WJMSCs strengthens the endurance of the cells, within stress conditions.

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Effects of Vitamin E, C and D Supplementation on Inflammation and Oxidative Stress in Streptozotocin-Induced Diabetic Mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000156 ◽

2013 ◽

Vol 83 (3) ◽

pp. 168-175 ◽

Cited By ~ 19

Author(s):

Agnieszka Greń

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Vitamin C ◽

Diabetic Mice ◽

Concurrent Administration ◽

Diabetes Group ◽

C 50 ◽

And Oxidative Stress ◽

Preventive Role

Background: The main objective of the study was to analyze the potential ability of vitamins E, C, and D, used as nutritional supplements, in averting inflammation and oxidative stress in the course of diabetes mellitus. Methods: Male mice were divided into eight groups. Diabetes was induced (groups II, VI, VII, and VIII) by an intraperitoneal injection of streptozotocin (STZ). The third and sixth groups were given vitamin C (50 mg/kg) 3 times per week, the fourth and seventh groups were given vitamin E (300 mg/kg) 3 times per week, and the fifth and eight groups were given vitamin D (2000 IU/day). Interleukin-6 levels were measured in serum. Glutathione (GSH) levels and glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activity were measured in the liver tissue. Results: STZ resulted in a significant decrease in all tested enzymes and glutathione levels, and an increase in IL-6 level in comparison to control animals (p < 0.05). Mice treated with vitamins had a significantly (p < 0.05) higher content of enzymes and glutathione in liver than diabetic mice, however IL-6 concentration showed a significant decrease. Concurrent administration of STZ and vitamins caused a significant increase (compared to the diabetes group) in SOD, CAT, GPx, GSH content, and a decrease in IL-6 levels (p < 0.05). Conclusion: These results indicate the preventive role of vitamin C, E, and D against STZ-induced diabetic oxidative stress and inflammation. Hence these vitamins could be used as an adjuvant therapy for the prevention and/or management of diabetes.

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Impact of Polyphenolic-Food on Longevity: An Elixir of Life. An Overview

Antioxidants ◽

10.3390/antiox10040507 ◽

2021 ◽

Vol 10 (4) ◽

pp. 507

Author(s):

Rosaria Meccariello ◽

Stefania D’Angelo

Keyword(s):

Oxidative Stress ◽

Food Sources ◽

Preventive Action ◽

Nutritional Interventions ◽

Beneficial Effects ◽

Age Related ◽

Macromolecular Damage ◽

And Oxidative Stress

Aging and, particularly, the onset of age-related diseases are associated with tissue dysfunction and macromolecular damage, some of which can be attributed to accumulation of oxidative damage. Recently, growing interest has emerged on the beneficial effects of plant-based diets for the prevention of chronic diseases including obesity, diabetes, and cardiovascular disease. Several studies collectively suggests that the intake of polyphenols and their major food sources may exert beneficial effects on improving insulin resistance and related diabetes risk factors, such as inflammation and oxidative stress. They are the most abundant antioxidants in the diet, and their intake has been associated with a reduced aging in humans. Polyphenolic intake has been shown to be effective at ameliorating several age-related phenotypes, including oxidative stress, inflammation, impaired proteostasis, and cellular senescence, both in vitro and in vivo. In this paper, effects of these phytochemicals (either pure forms or polyphenolic-food) are reviewed and summarized according to affected cellular signaling pathways. Finally, the effectiveness of the anti-aging preventive action of nutritional interventions based on diets rich in polyphenolic food, such as the diets of the Blue zones, are discussed.

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