NATURAL PROLIFERATIVE PHASE FROZEN EMBRYO TRANSFERS (NPP-FET) – A NEW APPROACH THAT FACILITATES SCHEDULING WITHOUT HINDERING PREGNANCY OUTCOMES

OBJECTIVE: Nowadays, fresh embryo transfers and frozen embryo transfers are frequently employed in the treatment of in vitro fertilization. This study aims to compare the pregnancy outcomes in patients who underwent fresh embryo transfers and frozen embryo transfers. STUDY DESIGN: All patients who underwent fresh embryo transfers and frozen embryo transfers at the in vitro fertilization center, Ondokuz Mayis University between 2010 and 2017 were screened retrospectively and the pregnancy results were evaluated at one-year follow-up. The study included a total of 912 transfers, 679 of which were fresh embryo transfers and 233 were fresh embryo transfers, in 756 patients. Comparisons were made in terms of biochemical pregnancy, clinical pregnancy rate, ongoing pregnancy, and live birth rate.RESULTS: Ectopic pregnancy, biochemical pregnancy, and abortus in fresh embryo transfers were found to be significantly more than that in frozen embryo transfers (p=0.001). However, no statistically significant difference in terms of clinical or ongoing pregnancy rate or live birth rate was observed. Birth weight was significantly lower in fresh embryo transfers than in frozen embryo transfers (p=0.001, p= 0.031). Multiple pregnancies preeclampsia, preterm labor, and placental abruption did not show a statistically significant difference in fresh embryo transfers and frozen embryo transfers. Yet, gestational diabetes was significantly more in frozen embryo transfers (p=0.011).CONCLUSIONS: Early pregnancy complications in fresh embryo transfers are higher than that in frozen embryo transfers. In terms of neonatal results, higher birth weight and gestational diabetes are more prevalent in frozen embryo transfers. In this study, it has been shown that fresh embryo transfers are more often associated with negative pregnancy outcomes. frozen embryo transfers can be better for pregnancy results

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The O–C diagrams of minor planets − a new approach to modelling the rotation

International Astronomical Union Colloquium ◽

10.1017/s0252921100031407 ◽

1999 ◽

Vol 173 ◽

pp. 185-188

Author(s):

Gy. Szabó ◽

K. Sárneczky ◽

L.L. Kiss

Keyword(s):

Error Analysis ◽

Time Dependence ◽

Theoretical Work ◽

Minor Planet ◽

Minor Planets ◽

New Approach ◽

Preliminary Results ◽

Ccd Observations

AbstractA widely used tool in studying quasi-monoperiodic processes is the O–C diagram. This paper deals with the application of this diagram in minor planet studies. The main difference between our approach and the classical O–C diagram is that we transform the epoch (=time) dependence into the geocentric longitude domain. We outline a rotation modelling using this modified O–C and illustrate the abilities with detailed error analysis. The primary assumption, that the monotonity and the shape of this diagram is (almost) independent of the geometry of the asteroids is discussed and tested. The monotonity enables an unambiguous distinction between the prograde and retrograde rotation, thus the four-fold (or in some cases the two-fold) ambiguities can be avoided. This turned out to be the main advantage of the O–C examination. As an extension to the theoretical work, we present some preliminary results on 1727 Mette based on new CCD observations.

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Scanning and Transmission Electron Microscopy of Endomentrium in Women Wearing TCu-380-A IUD 's

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080754 ◽

1977 ◽

Vol 35 ◽

pp. 666-667

Author(s):

A. Gonzalez Angulo ◽

R. Berlioz ◽

R. Aznar

Keyword(s):

Copper Wire ◽

Uterine Cavity ◽

Morphological Evidence ◽

Secretory Phase ◽

Ultrastructural Studies ◽

Proliferative Phase ◽

Contraceptive Devices ◽

Transmission Electron ◽

Intrauterine Contraceptive ◽

Myelin Figure

Recent ultrastructural studies on endometrial tissues from women wearing copper, wire intrauterine devices have disclosed morphological evidence of impaired glycogen degradation and secretion resulting in interference with the viability of blastocysts. Reduced microapocrine secretion observed with the scanning electron microscope supports this (1). In addition, organelle modifications have been observed in the epithelial cells of these women. The changes are seen in biopsies taken in the proliferative phase of the cycle and consist of mitochondrial vacuolation and myelin figure formation. These modifications disappear in the secretory phase and therefore have been regarded as reversible (2).The aim of the present studies was to investigate surface epithelial changes as well as organelle modifications in relation to the site of contact with an IUD that releases greater amounts of copper. Endometrial tissue was obtained from the uterine cavity of four young women wearing TCu-380-A intrauterine contraceptive devices for 4-6 weeks.

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A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073933 ◽

1973 ◽

Vol 31 ◽

pp. 698-699

Author(s):

V. Mizuhira ◽

Y. Futaesaku

Keyword(s):

Cross Section ◽

Tannic Acid ◽

Elastic Fiber ◽

The Other ◽

New Approach ◽

Tissue Block ◽

Active Reaction ◽

The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

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The Effects of Estradiol on Chronically Damaged Human Fallopian Tubes: A SEM Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119934 ◽

1985 ◽

Vol 43 ◽

pp. 648-649

Author(s):

A. Toledo ◽

G. Stoelk ◽

M. Yussman ◽

R.P. Apkarian

Keyword(s):

Menstrual Cycle ◽

Epithelial Cell ◽

Secretory Cell ◽

Estrogen Therapy ◽

Fallopian Tubes ◽

Cell Surfaces ◽

Proliferative Phase ◽

Cyclic Patterns ◽

Sem Study ◽

The U.S

Today it is estimated that one of every three women in the U.S. will have problems achieving pregnancy. 20-30% of these women will have some form of oviductal problems as the etiology of their infertility. Chronically damaged oviducts present problems with loss of both ciliary and microvillar epithelial cell surfaces. Estradiol is known to influence cyclic patterns in secretory cell microvilli and tubal ciliogenesis, The purpose of this study was to assess whether estrogen therapy could stimulate ciliogenesis in chronically damaged human fallopian tubes.Tissues from large hydrosalpinges were obtained from six women undergoing tuboplastic repair while in the early proliferative phase of fheir menstrual cycle. In each case the damaged tissue was rinsed in heparinized Ringers-lactate and quartered.

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Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120266 ◽

1985 ◽

Vol 43 ◽

pp. 714-715

Author(s):

K. Chien ◽

R. Van de Velde ◽

I.P. Shintaku ◽

A.F. Sassoon

Keyword(s):

Cell Monolayer ◽

Cell Types ◽

Surface Antigens ◽

Immunoperoxidase Method ◽

Reaction Step ◽

Hot Plate ◽

Air Drying ◽

New Approach ◽

Cell Monolayers ◽

Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

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TEM study of a biofilm on copper corrosion

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100163563 ◽

1996 ◽

Vol 54 ◽

pp. 220-221

Author(s):

W. A. Chiou ◽

N. Kohyama ◽

B. Little ◽

P. Wagner ◽

M. Meshii

Keyword(s):

Marine Bacterium ◽

Culture Medium ◽

Copper Alloys ◽

Pure Copper ◽

Localized Corrosion ◽

Natural Seawater ◽

Copper Corrosion ◽

New Approach ◽

The Past ◽

Copper Tolerant

The corrosion of copper and copper alloys in a marine environment is of great concern because of their widespread use in heat exchangers and steam condensers in which natural seawater is the coolant. It has become increasingly evident that microorganisms play an important role in the corrosion of a number of metals and alloys under a variety of environments. For the past 15 years the use of SEM has proven to be useful in studying biofilms and spatial relationships between bacteria and localized corrosion of metals. Little information, however, has been obtained using TEM capitalizing on its higher spacial resolution and the transmission observation of interfaces. The research presented herein is the first step of this new approach in studying the corrosion with biological influence in pure copper.Commercially produced copper (Cu, 99%) foils of approximately 120 μm thick exposed to a copper-tolerant marine bacterium, Oceanospirillum, and an abiotic culture medium were subsampled (1 cm × 1 cm) for this study along with unexposed control samples.

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