Therapeutic potential of monoterpene α-thujone, the main compound of Thuja occidentalis L. essential oil, against malignant glioblastoma multiforme cells in vitro

Investigation of the Essential Oil of Ormenis africana from Morocco: Revision of Chemical Composition and Antibacterial Activity

Universities' Journal of Phytochemistry and Ayurvedic Heights ◽

10.51129/ujpah-june2021-30-1(5) ◽

2021 ◽

Vol 1 (30) ◽

pp. 34-38

Author(s):

El Hanbali F Barrero A.F

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oil Composition ◽

Proteus Vulgaris ◽

Oil Composition ◽

Gram Negative ◽

Main Compound ◽

Oxygenated Compounds ◽

Aerial Parts

Abstract- The essential oil composition from the aerial parts of Ormenis africana (Asteraceae), an endemic species from Morocco, has been investigated by GC/MS. A total of 31 compounds were identified, representing 77%. After fractionation by column chromatography, the main compound was isolated and its structure elucidated by NMR spectroscopy. The essential oil was dominated by oxygenated compounds with spathulenol (45.8%) followed by camphor (7.1%), -cadinol (5.9%) and -bisabolol (5.9%) as the main compounds. This oil can be classified as spathulenol-type according to its spathulenol content. In vitro the antibacterial activity of the whole essential oil against three Gram positive (Bacillus cereus, Enterococcus faecalis, Streptococcus C) bacteria and three Gram negative (Proteus vulgaris, Escherichia coli, Pseudomonas aeroginosa) bacteria, showed significant results. Keywords: Asteraceae, Ormenis africana, Essential oil, Spathulenol, Antibacterial activity.

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The Essential Oil of Artemisia argyi H.Lév. and Vaniot Attenuates NLRP3 Inflammasome Activation in THP-1 Cells

Frontiers in Pharmacology ◽

10.3389/fphar.2021.712907 ◽

2021 ◽

Vol 12 ◽

Author(s):

Pengxiao Chen ◽

Qi Bai ◽

Yanting Wu ◽

Qiongzhen Zeng ◽

Xiaowei Song ◽

...

Keyword(s):

Essential Oil ◽

Nlrp3 Inflammasome ◽

Therapeutic Potential ◽

Inflammasome Activation ◽

Caspase 1 ◽

Artemisia Argyi ◽

Nlrp3 Inflammasome Activation ◽

Long Time

Artemisia argyi H. Lév. and Vaniot is a traditional medical herb that has been used for a long time in China and other Asian counties. Essential oil is the main active fraction of Artemisia argyi H. Lév. and Vaniot, and its anti-inflammatory potential has been observed in vitro and in vivo. Here, we found that the essential oil of Artemisia argyi H. Lév. and Vaniot (EOAA) inhibited monosodium urate (MSU)- and nigericin-induced NLRP3 inflammasome activation. EOAA suppressed caspase-1 and IL-1β processing and pyroptosis. NF-κB p65 phosphorylation and translocation were also inhibited. In addition, EOAA suppressed nigericin-induced NLRP3 inflammasome activation without blocking ASC oligomerization, suggesting that it may inhibit NLRP3 inflammasome activation by preventing caspase-1 processing. Our study thus indicates that EOAA inhibits NLRP3 inflammasome activation and has therapeutic potential against NLRP3-driven diseases.

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Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil

Molecules ◽

10.3390/molecules24213880 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3880 ◽

Cited By ~ 2

Author(s):

Nidal Jaradat ◽

Nawaf Al-Maharik

Keyword(s):

Essential Oil ◽

Therapeutic Potential ◽

Chemical Constituents ◽

Metabolic Enzymes ◽

Positive Control ◽

Metabolic Enzyme ◽

Colon Cancer Cell Lines ◽

Ic50 Values ◽

Pharmacologically Active

The present study aimed to identify the chemical constituents and to assess the in-vitro, antimicrobial, anticancer, antioxidant, metabolic enzymes and cyclooxygenase (COX) inhibitory properties of essential oil (EO) of Stachys viticina Boiss. leaves. The S. viticina EO was isolated and identified using microwave-ultrasonic and GC-MS techniques, respectively. Fifty-two compounds were identified, of which endo-borneol was the major component, followed by eucalyptol and epizonarene. The EO was evaluated against a panel of in-vitro bioassays. The EO displayed antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Epidermophyton floccosum, with MIC values of 0.039, 0.078 and 0.78 mg/mL, respectively. The EO exhibited cytotoxicity against HeLa (cervical adenocarcinoma) and Colo-205 (colon) cancer cell lines with percentages of inhibition of 95% and 90%, for EO concentrations of 1.25 and 0.5 mg/mL, respectively. Furthermore, it showed metabolic enzyme (α-amylase, α-glucosidase, and lipase) inhibitory (IC50 = 45.22 ± 1.1, 63.09 ± 0.26, 501.18 ± 0.38 µg/mL, respectively) and antioxidant activity, with an IC50 value of 19.95 ± 2.08 µg/mL. Moreover, the S. viticina EO showed high cyclooxygenase inhibitory activity against COX-1 and COX-2 with IC50 values of 0.25 and 0.5 µg/mL, respectively, similar to those of the positive control (the NSAID etodolac). Outcomes amassed from this investigation illustrate that S. viticina EO represents a rich source of pharmacologically active molecules which can be further validated and explored clinically for its therapeutic potential and for the development and design of new natural therapeutic preparations.

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Essential Oil of Cymbopogon Citratus Grown in Umuahia: A Viable Candidate for Anti-Inflammatory and Antioxidant Therapy

Acta Universitatis Cibiniensis Series E Food Technology ◽

10.2478/aucft-2021-0001 ◽

2021 ◽

Vol 25 (1) ◽

pp. 1-14

Author(s):

Uchechi Bliss Onyedikachi ◽

Favour Matthew Awah ◽

Charles Nnanna Chukwu ◽

Emmanuel Ejiofor

Keyword(s):

Essential Oil ◽

Vitamin C ◽

Therapeutic Potential ◽

Preliminary Evidence ◽

Gas Chromatography Mass Spectrometry ◽

Cymbopogon Citratus ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Anti Inflammatory

Abstract The essential oils of Cymbopogon citratus (EOCC) has found use in medicine, food and chemical industry. This study attempts to provide evidence of its suitability for antioxidant and anti-inflammatory therapy. Total phenol and total flavonoid of EOCC was 49.83±0.39mg GAE/g of extract and 352.82±3.45 µg QEC/g of extract respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of its essential oil (EOCC) showed 25 peaks with myrcenyl acetate (9.703%), caryophyllene (8.997%), citronella (6.383%) been the most abundant. The in vitro anti-inflammatory assay using human red blood cell (HRBC) membrane stabilization shows that at 200µg/mL, the percentage inhibition of EOCC was significantly higher compared to diclofenac both for heat-induced and hypotonic induced haemolysis. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed a comparable and dose-dependent increase from 50 to 400 μg/mL in relation to vitamin C. Half maximal inhibitory concentration (IC50) of EOCC (73.16±12.89 μg/mL and 656.01±0.01 μmol Fe (II)/L) was remarkably higher compared to that of vitamin C (69.09±4.52 μg/mL and 246.79±0.01 μmol Fe (II)/L) both for DPPH and FRAP assays respectively. In conclusion, results from this study establish preliminary evidence on the therapeutic potential of EOCC in managing inflammation and oxidative stress caused by free radicals.

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Herbicidal Activity of Thymbra capitata (L.) Cav. Essential Oil

Molecules ◽

10.3390/molecules25122832 ◽

2020 ◽

Vol 25 (12) ◽

pp. 2832 ◽

Cited By ~ 2

Author(s):

Mercedes Verdeguer ◽

Natalia Torres-Pagan ◽

Marta Muñoz ◽

Amira Jouini ◽

Susana García-Plasencia ◽

...

Keyword(s):

Essential Oil ◽

Chenopodium Album ◽

Amaranthus Retroflexus ◽

Herbicidal Activity ◽

In Vitro Assays ◽

Main Compound ◽

Setaria Verticillata ◽

Solanum Nigrum L ◽

Soil Seedbank

The bioherbicidal potential of Thymbra capitata (L.) Cav. essential oil (EO) and its main compound carvacrol was investigated. In in vitro assays, the EO blocked the germination and seedling growth of Erigeron canadensis L., Sonchus oleraceus (L.) L., and Chenopodium album L. at 0.125 µL/mL, of Setaria verticillata (L.) P.Beauv., Avena fatua L., and Solanum nigrum L. at 0.5 µL/mL, of Amaranthus retroflexus L. at 1 µL/mL and of Portulaca oleracea L., and Echinochloa crus-galli (L.) P.Beauv. at 2 µL/mL. Under greenhouse conditions, T. capitata EO was tested towards the emergent weeds from a soil seedbank in pre and post emergence, showing strong herbicidal potential in both assays at 4 µL/mL. In addition, T. capitata EO, applied by spraying, was tested against P. oleracea, A. fatua and E. crus-galli. The species showed different sensibility to the EO, being E. crus-galli the most resistant. Experiments were performed against A. fatua testing T. capitata EO and carvacrol applied by spraying or by irrigation. It was verified that the EO was more active at the same doses in monocotyledons applied by irrigation and in dicotyledons applied by spraying. Carvacrol effects on Arabidopsis root morphology were also studied.

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Antileishmanial Activity and Influence on Mitochondria of the Essential Oil from Tagetes lucida Cav. and Its Main Component

Scientia Pharmaceutica ◽

10.3390/scipharm88030031 ◽

2020 ◽

Vol 88 (3) ◽

pp. 31

Author(s):

Lianet Monzote ◽

Yamilet Gutiérrez ◽

Laura Machin ◽

Katrin Staniek ◽

Ramón Scull ◽

...

Keyword(s):

Oxygen Consumption ◽

Membrane Potential ◽

Essential Oil ◽

Antileishmanial Activity ◽

Gas Chromatography Mass Spectrometry ◽

Main Compound ◽

J774 Cells ◽

Cytotoxicity Effects ◽

Main Component

Current antileishmanial drugs are toxic, expensive, and resistance to them has emerged. Several studies have focused on natural products as alternatives. In the present work, the chemical composition, in vitro antileishmanial activity, cytotoxicity effects, and the influence on mitochondrial function of the essential oil from Tagetes lucida Cav. was determined, as well its main compound estragole. Forty-nine compounds were detected in the oil by gas chromatography-mass spectrometry (GC-MS), of which estragole was the main constituent (97%). The oil showed inhibition of the promastigotes of L. tarentolae and L. amazonensis (IC50 = 61.4 and 118.8 µg/mL, respectively), decreased oxygen consumption of L. tarentolae, disrupted mitochondrial membrane potential in L. amazonensis, inhibitory activity on the intracellular amastigote of L. amazonensis (IC50 = 14.2 ± 1.6 µg/mL), and cytotoxicity values ranging from 80.8 to 156 µg/mL against murine macrophages and J774 cells. Estragole displayed higher activity on promastigotes (IC50 = 28.5 and 25.5 µg/mL, respectively), amastigotes (IC50 = 1.4 ± 0.1 µg/mL), and cytotoxicity values ranging from 20.6 to 14.5 µg/mL, respectively, while on mitochondria, it caused a decrease of the membrane potential but did not inhibit oxygen consumption. The potential antileishmanial activity of the essential oil from T. lucida and estragole makes these compounds favorable candidates for exploration in further studies.

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Chemical composition and antibacterial activity of essential oil of Senecio graciliflorus

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1-s.2265 ◽

2019 ◽

Vol 9 (1-s) ◽

pp. 98-100 ◽

Cited By ~ 1

Author(s):

B.C. Joshi ◽

Vinod Kumar ◽

Bhuwan Chandra ◽

N.D. Kandpal

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Therapeutic Potential ◽

Diffusion Method ◽

Bacterial Strains ◽

Antimicrobial Drugs ◽

Bacterial Diseases ◽

Aerial Parts ◽

Plant Constituents

Objective: Among natural products, essential oils and their contituents represent a structurally diverse group of organic compounds with significant antibacterial activity. This study is oriented to assess the therapeutic potential of plant constituents as new antimicrobial drugs. Methods: Aerial parts of Senecio graciliflorus was steam distilled and analysed by GC and GC-MS for the volatile constituents. Essential oil was studied for their antibacterial activity against Gram-positive and Gram-negative bacteria using agar well diffusion method. Results: The results revealed that α-pinene and α-thujene are the major constituents of S. graciliflorus oil. In vitro studies showed significant antimicrobial activity against bacterial strains. Conclusion: This study demonstrated that the oil significantly inhibits the growth of baceria. S. graciliflorus would be another alternative for developing new pharmaceuticals for prevention bacterial diseases and provide a direction for the study of naturally derived drugs. Keywords: Asteraceae, Senecio graciliflorus, Essential oil, α-Pinene, Antibacterial activity.

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Activity of Tea Tree (Melaleuca alternifolia) Essential Oil against L3 Larvae ofAnisakis simplex

BioMed Research International ◽

10.1155/2014/549510 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 12

Author(s):

Carlota Gómez-Rincón ◽

Elisa Langa ◽

Paula Murillo ◽

Marta Sofía Valero ◽

César Berzosa ◽

...

Keyword(s):

Essential Oil ◽

Therapeutic Potential ◽

Anisakis Simplex ◽

Ache Inhibitor ◽

Tea Tree Oil ◽

Melaleuca Alternifolia ◽

Tea Tree ◽

Dependent Inhibition ◽

Ld50 Value

Nematicidal activity ofMelaleuca alternifoliaessential oil, commonly known as tea tree oil (TTO), was assayedin vitroagainst L3 larvae ofAnisakis simplex. The results showed a mortality of 100% for concentrations between 7 and 10 μL/mL after 48 h of incubation, obtaining an LD50 value of 4.53 μL/mL after 24 hours and 4.27 μL/mL after 48 hours. Concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100 μL/mL. This fact suggests that TTO may act as an AChE inhibitor. Terpinen-4-ol was discarded as main larvicide compound as it did not show larvicidal or anticholinesterase activity. The data obtained suggest that the essential oil ofMelaleuca alternifoliamay have a great therapeutic potential for the treatment of human anisakiasis.

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