Determination of the nutritional and functional metabolites of Nanochloropsis gaditana produced in Algeria and evaluation of its antioxidant activity

2017 ◽  
Vol 7 (1) ◽  
pp. 13-20
Author(s):  
Asmaa Kherraf ◽  
Wafaa Tehami ◽  
Wahiba Boufeldja ◽  
Imène Yahla ◽  
Ghislaine Amira Dra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Tannic Acid ◽  
Reducing Power ◽  
Matter Content ◽  
Bioactive Substances ◽  
Fresh Matter ◽  
Nannochloropsis Gaditana ◽  
Methanolic Extracts ◽  
Gallic Acid Equivalent

The aim of this work was to valorize the microalgae Nannochloropsis gaditana grown in Algeria for the purpose of producing human and animal food supplements as well as bioactive substances. The microalga had a fresh matter content of 9.33±1.15%. The amount of ash (6.4 ± 0.23%) was high and reflected mineral richness. Total sugars represented 7.13±0.23%. Proteins and lipids represented 13.38±0.2% and 7±1%, respectively. The ethanolic (EENg) and methanolic (MENg) extracts obtained from 10 g of lyophilisates of N. gaditana represented respectively 24±1.65% and 14.2±0.7%. The evalu-ation of the polyphenols in these extracts showed interesting rates repre-senting 41.53±0.41 mg gallic acid equivalent (GAE)/g of EENg dry extract and 38.72±0.33 mg GAE/g for the MENg. The flavonoid contents were 19.82±1.65 and 18.61±1.45 mg quercetin equivalent (QE)/g respectively for the ethanol-ic and methanolic extracts of this microalgae. The condensed and hydrolyza-ble tannins represented respectively 0.35±0.04 and 0.63±0.09 mg of tannic acid/g of dry EENg. They also represented respectively 0.50±0.08 and 0.18±0.02 mg of tannic acid/g of dry MENg. The antioxidant activity generat-ed by the extracts EENg and MENg against 2,2-diphenyl-1-picrylhydrazil (DPPH ) and by the reducing power of iron (FRAP) seems appreciable com-pared to that of ascorbic acid. These results are in favor of the development of the microalgae sector constituting a futuristic vision to allow the development of the sector with a view to sustainability.

Determination of free phenolic acids and antioxidant activity of methanolic extracts obtained from fruits and leaves of Chenopodium album

2011 ◽  
Vol 126 (4) ◽  
pp. 1850-1855 ◽  
Author(s):  
Abdul Hafeez Laghari ◽  
Shahabuddin Memon ◽  
Aisha Nelofar ◽  
Khalid Mohammed Khan ◽  
Arfa Yasmin
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Chenopodium Album ◽  
Methanolic Extracts ◽  
Free Phenolic Acids

scholarly journals Antioxidant Activity, Total Phenols, Flavonoids and LCMS Profile of Chamaecrista hildebrandtii (Vatke) Lock and Clerodendrum rotundifolium (Oliv.)

2019 ◽  
pp. 1-11
Author(s):  
Rechab S. Odhiambo ◽  
Patrick G. Kareru ◽  
Erastus K. Mwangi ◽  
Daniel W. Onyango
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Tannic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Total Phenols ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Significant Difference ◽  
Different Types

Aims: The purpose of the study was to determine the antioxidant activity, quantify total phenols and total flavonoids and characterize the secondary metabolites present in methanolic extracts of Chamaecrista hildebrandtii and Clerodendrum rotundifolium using liquid chromatography coupled to mass spectrometry (LC-MS). Methodology: The total phenol and flavonoid contents were determined spectrophotometrically while the antioxidant activity was evaluated using the 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) free radical scavenging method. The secondary metabolites present in the methanolic leaves extracts were evaluated using LC-MS. Results: The extracts of C. hildebrandtii showed a significantly higher antioxidant activity (IC50 = 8.7 mg/mL) compared to C. rotundifolium (IC50= 28.5 mg/mL). Both methanolic extracts of C. hildebrandtii and C. rotundifolium had common and different types of flavonoids such as quercetin, rutin, (+)-catechin 3-O-gallate and luteolin 6-C-glucoside among others that could be responsible for the observed antioxidant activity. The total phenolic content of C. hildebrandtii (1.33±0.07 mg/g tannic acid equivalents) was significantly higher than that of C. rotundifolium (0.25±0.00 mg/g tannic acid equivalents). However, there was no statistically significant difference (p>0.05) in total flavonoid content of C. hildebrandtii (2.69±0.33 mg/g catechin equivalents) and C. rotundifolium (2.36±0.16 mg/g catechin equivalents). Conclusion: The results of the present study suggested that the good antioxidant activity exhibited by C. hildebrandtii may probably have been brought about by various secondary metabolites functioning in synergy.

Evaluation of the antioxidant activity of phenols and tannic acid determination with Mn3O4 nano-octahedrons as an oxidase mimic

2015 ◽  
Vol 7 (20) ◽  
pp. 8640-8646 ◽  
Author(s):  
Xiaodan Zhang ◽  
Yuming Huang
Keyword(s):  
Antioxidant Activity ◽  
Tannic Acid ◽  
Preliminary Assessment ◽  
Acid Determination

Mn3O4 nano-octahedrons as an effective oxidase mimic for the preliminary assessment of phenols' antioxidant activity and visual determination of tannic acid were demonstrated.

scholarly journals DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

2017 ◽  
Vol 10 (12) ◽  
pp. 274 ◽  
Author(s):  
Satya Lakshmi S
Keyword(s):  
Antioxidant Activity ◽  
Marine Algae ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Dpph Method ◽  
Anti Inflammatory

  Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

Study on Extraction of Natural Black Soya Bean Peptide and Antioxidant Activity

2013 ◽  
Vol 634-638 ◽  
pp. 1066-1070 ◽  
Author(s):  
Yan Fang Zhang ◽  
Chun Ling Xiao ◽  
Xin Jia ◽  
Qiong Jie Li ◽  
Yuan Yuan Xue ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Oxidation Stability ◽  
Reducing Power ◽  
Soya Bean ◽  
Black Beans ◽  
Liquid Material ◽  
Peptide Oxidation ◽  
Better Than

With black beans as raw materials, different factors on natural black beans peptide yield influence, and through the establishment of DPPH • system, OH • system, reducing power system measurement for the determination of different concentration of black soya bean peptide oxidation stability. The results showed that the liquid material ratio 17.5,leaching 42°C,extracting 55 min, black beans peptide extraction is 2.386 mg/g. Black beans peptide in various system all have certain oxidative stability, in the concentration of 0.04mg/ml to 74.05% clearance of DPPH• , in 0.06mg/ml concentration of OH•clearance rate is 80.48%, 0.08 mg/ml concentrations reduction force is 0.745, and in these three system natural black beans peptide oxidation stability are better than those of contrast VC is strong .

scholarly journals Content of anthocyanins of seed of Phaseolus vulgaris

2020 ◽  
pp. 7-12
Author(s):  
Rafael Manuel de Jesús Mex-Morales ◽  
Patricia Margarita Garma-Quen ◽  
María Magali Guillen-Morales ◽  
María Isabel Novelo-Pérez
Keyword(s):  
Phaseolus Vulgaris ◽  
Reducing Power ◽  
Total Content ◽  
Aqueous Extracts ◽  
Phaseolus Vulgaris L ◽  
Bioactive Substances ◽  
Length Width ◽  
Reducing Activity ◽  
The Common

The common bean (Phaseolus vulgaris L) is an important food in Mexico that provides both macronutrients and bioactive substances that are beneficial for the health of its consumers, in particular it provides polyphenolic compounds such as anthocyanins that make it a functional food; the objective of the present work was to evaluate the content of polyphenols and their ferric iron reducing activity of beans grown in the Municipality of Hopelchen, Campeche (Mexico) to characterize their agronomic and alimentary value. The morphometric parameters of length, width, thickness, weight and volume were measured; An aqueous maceration of the bean seeds was carried out in an acid medium (0.1 M HCl) and the content of polyphenols (Folin Ciocalteu Method), anthocyanins (visible spectrophotometry) and determination of the Reducing Iron Power (FRAP, by the method of TPTZ) of the aqueous extracts. It was found that the concentration of anthocyanins correlates with the total content of polyphenols and with the reducing activity of the ferric ion, the aqueous extracts obtained had a content of total phenols of 413 mg EAG/100g of seed, of anthocyanins of 42 mg MGE/100 g of seed and an iron reducing power of 10.26 mol of Fe2+/g of seed. The morphometric characteristics and the polyphenol content of the Hopelchén bean demonstrate that the harvested grain is of good quality and corresponds to the expected values for this food.

scholarly journals Flavonoids from aerial part of Algerian Ajuga iva (L.) schreb.: The HPLC-UV analysis and Antioxidant capacity

2021 ◽  
pp. 23-34
Author(s):  
Fadhela Boukada ◽  
Boumediene Meddah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Aerial Part ◽  
Reducing Power ◽  
Thiobarbituric Acid ◽  
Dry Extract ◽  
Antioxidant Agents ◽  
Gallic Acid Equivalent

The study deals with the evaluation of the antioxidant capacity of extracts from the aerial part of Algerian Ajuga iva. Extraction of flavonoids was carried out by 85% of methanol, then the crude extract was successively separated with ethyl acetate, butanol, and water. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl, reducing power, and thiobarbituric acid reacting substances assays. Extracts are subject to HPLC-UV analysis. The average total phenol contents of extracts vary between 3.87 ± 0.17 and 149.74 ± 3.94 (gallic acid equivalent per gram of dry extract). Furthermore, tested extracts exhibited a broad range of flavonoid contents varying from 1.54 ± 0.09 to 41.18 ± 1.03 (catechin equivalent per gram of dry extract). Butanol and ethyl acetate fractions displayed the highest antioxidant activity. A good correlation between the phenolic and flavonoid contents and the antioxidant activity was observed. Rutin, caffeic acid, quercetin, p-coumaric acid, luteolin, and cinnamic acid were present in the extracts. The plant could be a potential source of antioxidant agents.

scholarly journals Comparative Analysis of the Antioxidant Activity of Cassia fistula Extracts

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Irshad ◽  
Md. Zafaryab ◽  
Man Singh ◽  
M. Moshahid A. Rizvi
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Cassia Fistula ◽  
Antioxidant Power ◽  
Methanolic Extracts ◽  
Hexane Extracts ◽  
Phenolic And Flavonoid ◽  
Reducing Capacity

Antioxidant potential of various extracts of Cassia fistula was determined by the DPPH, FRAP, Fe3+ reducing power, and hydrogen peroxide scavenging assay. Methanolic extracts of Cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. The order of antioxidant activity in Cassia fistula extracts displayed from higher to lower level as methanolic extracts of pulp, methanolic extracts of seed, hexane extracts of pulp, and hexane extracts of seed. The antioxidant potential of Cassia fistula extracts significantly correlated () with the phenolic content of the methanolic extracts. Ascorbic acid taken as control showed highest antioxidant power in the present study.

scholarly journals ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC FUNGI FROM CALOTROPIS PROCERA FOR THEIR ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 10 (3) ◽  
pp. 254 ◽  
Author(s):  
Vipin Nagda ◽  
Archana Gajbhiye ◽  
Dinesh Kumar Chhatwani
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Aspergillus Species ◽  
Calotropis Procera ◽  
Penicillium Species ◽  
Methanolic Extracts ◽  
Isolation And Characterization ◽  
Radical Scavenging Assay

ABSTRACTObjective: To investigate the antioxidant activity of methanolic extract of endophytic fungi isolated from Calotropis procera plant.Methods: The endophytic fungi were isolated from explants of leaf, stem, and flower of C. procera on potato dextrose broth (PDB) medium. The fungalisolates were mass cultured in PDB. The methanolic extracts of these fungi were prepared and evaluated for the antioxidant activity by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and reducing power assay. The extracts were characterized for the presence of phenolics andflavonoids.Results: A total of 8 endophytic fungi were isolated from C. procera explants. Out of these 8 fungal isolates, the crude extracts of two fungi, i.e. CPIMR-2and CPIL-1 showed positive antioxidant activity. From the morphological characteristics, the isolates CPIMR-2 and CPIL-1 were identified as Penicilliumspp. and Aspergillus spp., respectively. Both the isolates were found to possess antioxidant potential with % inhibition value of 81.16% and 80.97%in the DPPH radical scavenging assay. The phytochemical screening of the methanolic extracts showed the presence of phenolics and flavonoids. Thetotal flavonoids content in Penicillium species and Aspergillus species were found to be 130.50 μg/mg and 94.91 μg/mg of extracts, respectively. Thetotal phenolic content was found to be 9.16 μg/mg and 12.13 μg/mg of extracts in Penicillium species and Aspergillus species, respectively.Conclusions: Endophtyic fungi were found to be present in medicinal value plant C. procera. The probable bioactive component for antioxidantactivity possessed by the fungi would be the flavonoids and phenolics. These metabolite produced by endophytic fungi from C. procera need to beexplored further as potential source of novel natural antioxidant compound.Keywords: Endophytes, Calotropis procera, Antioxidant, Radical scavenging, Reducing power, Flavonoids.

scholarly journals Influence of strawberry and raspberry consumption on the antioxidant status of human body

2006 ◽  
Vol 12 (3) ◽  
Author(s):  
A. Szenthe ◽  
É. Stefanovits-Bányai ◽  
A. Blazovics ◽  
A. Hegedűs ◽  
R. Engel ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Dry Matter ◽  
Reducing Power ◽  
Mineral Nutrients ◽  
Early Spring ◽  
Matter Content ◽  
Dry Matter Content

Several parameters influencing the antioxidant capacity were measured in our experiments to compare strawberry and raspberry cultivars. The analysed cultivars were: 'Honeoye', 'Elsanta', 'Onebor (strawberries) and 'Glen Ample', Fertődi zamatos', 'Mailing Expoit' (raspberries). Besides the determination of dry matter content the content of glucose, fructose, total phenol and anthocyanine, the reducing power, H-donating activity and total scavenger capacity were measured as well as the compound of mineral nutrients. Among the species and the cultivars, differences were obtained. These results call the attention to the features of cultivars and to count on the antioxidant-capacity describing values in case of consuming these fruits. In early spring and summer the consumption of strawberry and raspberry has a beneficial contribution to the antioxidant status of our body. In this respect these are more valuable than the year-long consumable apple. Accordingly, our measurements showed higher amount of sucrose, phenolic compounds and anthocyanine in raspberries, but strawberries contained more glucose and fructose and had stronger H-donating activity. It is worth to keep in mind that e.g. which cultivar contains more of certain compounds because of the strictly determined allowable dose in some diseases (e.g. diabetes).

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